Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14365 and BBR_RS14370 are separated by 27 nucleotides BBR_RS14370 and BBR_RS14375 are separated by 227 nucleotides
BBR_RS14365: BBR_RS14365 - hypothetical protein, at 996,344 to 997,516
_RS14365
BBR_RS14370: BBR_RS14370 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 997,544 to 998,878
_RS14370
BBR_RS14375: BBR_RS14375 - acetate kinase, at 999,106 to 1,000,335
_RS14375
Position (kb) 997
998
999 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 996.565 kb on + strand, within BBR_RS14365 at 996.578 kb on - strand, within BBR_RS14365 at 996.616 kb on + strand, within BBR_RS14365 at 996.616 kb on + strand, within BBR_RS14365 at 996.730 kb on - strand, within BBR_RS14365 at 996.742 kb on + strand, within BBR_RS14365 at 996.833 kb on + strand, within BBR_RS14365 at 996.848 kb on + strand, within BBR_RS14365 at 996.928 kb on + strand, within BBR_RS14365 at 996.932 kb on - strand, within BBR_RS14365 at 997.011 kb on + strand, within BBR_RS14365 at 997.021 kb on + strand, within BBR_RS14365 at 997.021 kb on + strand, within BBR_RS14365 at 997.029 kb on - strand, within BBR_RS14365 at 997.143 kb on - strand, within BBR_RS14365 at 997.150 kb on - strand, within BBR_RS14365 at 997.150 kb on - strand, within BBR_RS14365 at 997.293 kb on + strand, within BBR_RS14365 at 997.344 kb on - strand, within BBR_RS14365 at 997.346 kb on + strand, within BBR_RS14365 at 997.423 kb on + strand at 997.423 kb on + strand at 997.510 kb on + strand at 997.510 kb on + strand at 997.548 kb on - strand at 997.564 kb on + strand at 997.750 kb on + strand, within BBR_RS14370 at 997.777 kb on - strand, within BBR_RS14370 at 997.779 kb on + strand, within BBR_RS14370 at 997.793 kb on - strand, within BBR_RS14370 at 997.853 kb on + strand, within BBR_RS14370 at 997.861 kb on - strand, within BBR_RS14370 at 997.872 kb on + strand, within BBR_RS14370 at 997.911 kb on + strand, within BBR_RS14370 at 997.954 kb on - strand, within BBR_RS14370 at 997.964 kb on - strand, within BBR_RS14370 at 998.056 kb on + strand, within BBR_RS14370 at 998.150 kb on + strand, within BBR_RS14370 at 998.150 kb on + strand, within BBR_RS14370 at 998.186 kb on + strand, within BBR_RS14370 at 998.187 kb on - strand, within BBR_RS14370 at 998.348 kb on + strand, within BBR_RS14370 at 998.358 kb on + strand, within BBR_RS14370 at 998.363 kb on - strand, within BBR_RS14370 at 998.555 kb on + strand, within BBR_RS14370 at 998.712 kb on + strand, within BBR_RS14370 at 998.718 kb on - strand, within BBR_RS14370 at 998.873 kb on + strand at 998.878 kb on + strand at 998.907 kb on + strand at 998.931 kb on + strand at 998.960 kb on - strand at 998.969 kb on + strand at 998.979 kb on + strand at 998.979 kb on + strand at 999.026 kb on + strand at 999.071 kb on - strand at 999.119 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 996,565 + BBR_RS14365 0.19 +0.7 996,578 - BBR_RS14365 0.20 -0.8 996,616 + BBR_RS14365 0.23 -0.2 996,616 + BBR_RS14365 0.23 -1.3 996,730 - BBR_RS14365 0.33 +0.5 996,742 + BBR_RS14365 0.34 -0.2 996,833 + BBR_RS14365 0.42 +0.1 996,848 + BBR_RS14365 0.43 +0.3 996,928 + BBR_RS14365 0.50 -1.1 996,932 - BBR_RS14365 0.50 -0.0 997,011 + BBR_RS14365 0.57 +1.1 997,021 + BBR_RS14365 0.58 +0.2 997,021 + BBR_RS14365 0.58 +0.2 997,029 - BBR_RS14365 0.58 -1.1 997,143 - BBR_RS14365 0.68 +1.2 997,150 - BBR_RS14365 0.69 +0.4 997,150 - BBR_RS14365 0.69 +0.8 997,293 + BBR_RS14365 0.81 +1.5 997,344 - BBR_RS14365 0.85 -0.0 997,346 + BBR_RS14365 0.85 -0.2 997,423 + +0.2 997,423 + -0.2 997,510 + -0.4 997,510 + -2.2 997,548 - -0.1 997,564 + +0.5 997,750 + BBR_RS14370 0.15 -1.2 997,777 - BBR_RS14370 0.17 -1.1 997,779 + BBR_RS14370 0.18 +0.9 997,793 - BBR_RS14370 0.19 +1.5 997,853 + BBR_RS14370 0.23 -0.3 997,861 - BBR_RS14370 0.24 +1.5 997,872 + BBR_RS14370 0.25 +0.5 997,911 + BBR_RS14370 0.27 -0.7 997,954 - BBR_RS14370 0.31 -2.2 997,964 - BBR_RS14370 0.31 +0.9 998,056 + BBR_RS14370 0.38 -0.7 998,150 + BBR_RS14370 0.45 +0.9 998,150 + BBR_RS14370 0.45 -0.9 998,186 + BBR_RS14370 0.48 +0.9 998,187 - BBR_RS14370 0.48 +1.2 998,348 + BBR_RS14370 0.60 +1.1 998,358 + BBR_RS14370 0.61 -0.5 998,363 - BBR_RS14370 0.61 +1.5 998,555 + BBR_RS14370 0.76 +0.2 998,712 + BBR_RS14370 0.87 -0.3 998,718 - BBR_RS14370 0.88 +0.3 998,873 + -0.2 998,878 + -0.0 998,907 + +0.8 998,931 + -1.0 998,960 - +0.6 998,969 + +0.1 998,979 + +1.1 998,979 + -2.8 999,026 + +0.9 999,071 - +0.2 999,119 + -0.5
Or see this region's nucleotide sequence