Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14260 and BBR_RS14265 are separated by 157 nucleotides BBR_RS14265 and BBR_RS14270 are separated by 104 nucleotides
BBR_RS14260: BBR_RS14260 - ABC transporter ATP-binding protein, at 973,574 to 974,446
_RS14260
BBR_RS14265: BBR_RS14265 - Solute-binding protein of ABC transporter system for metals, at 974,604 to 975,776
_RS14265
BBR_RS14270: BBR_RS14270 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 975,881 to 976,759
_RS14270
Position (kb) 974
975
976 Strain fitness (log2 ratio) -2
-1
0
1
2 at 973.727 kb on - strand, within BBR_RS14260 at 973.761 kb on - strand, within BBR_RS14260 at 974.456 kb on - strand at 974.523 kb on - strand at 974.562 kb on + strand at 974.584 kb on - strand at 974.608 kb on - strand at 974.628 kb on - strand at 974.646 kb on - strand at 974.648 kb on + strand at 974.659 kb on + strand at 974.662 kb on - strand at 974.662 kb on - strand at 974.682 kb on - strand at 974.738 kb on - strand, within BBR_RS14265 at 974.776 kb on - strand, within BBR_RS14265 at 974.834 kb on + strand, within BBR_RS14265 at 974.841 kb on + strand, within BBR_RS14265 at 974.860 kb on - strand, within BBR_RS14265 at 974.893 kb on - strand, within BBR_RS14265 at 974.903 kb on + strand, within BBR_RS14265 at 974.915 kb on - strand, within BBR_RS14265 at 974.926 kb on + strand, within BBR_RS14265 at 975.051 kb on + strand, within BBR_RS14265 at 975.102 kb on - strand, within BBR_RS14265 at 975.102 kb on - strand, within BBR_RS14265 at 975.113 kb on - strand, within BBR_RS14265 at 975.151 kb on + strand, within BBR_RS14265 at 975.159 kb on + strand, within BBR_RS14265 at 975.191 kb on - strand, within BBR_RS14265 at 975.288 kb on + strand, within BBR_RS14265 at 975.295 kb on + strand, within BBR_RS14265 at 975.296 kb on - strand, within BBR_RS14265 at 975.324 kb on + strand, within BBR_RS14265 at 975.469 kb on + strand, within BBR_RS14265 at 975.486 kb on + strand, within BBR_RS14265 at 975.489 kb on - strand, within BBR_RS14265 at 975.489 kb on - strand, within BBR_RS14265 at 975.516 kb on - strand, within BBR_RS14265 at 975.611 kb on + strand, within BBR_RS14265 at 975.674 kb on - strand at 975.716 kb on + strand at 975.818 kb on + strand at 975.860 kb on + strand at 975.860 kb on + strand at 975.875 kb on + strand at 975.875 kb on + strand at 975.875 kb on + strand at 976.749 kb on + strand at 976.757 kb on - strand at 976.766 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 973,727 - BBR_RS14260 0.18 -0.6 973,761 - BBR_RS14260 0.21 -0.7 974,456 - -0.6 974,523 - -0.7 974,562 + +0.6 974,584 - +0.0 974,608 - -0.4 974,628 - -0.0 974,646 - +0.0 974,648 + -0.2 974,659 + +0.1 974,662 - -0.7 974,662 - -0.7 974,682 - -0.3 974,738 - BBR_RS14265 0.11 -0.4 974,776 - BBR_RS14265 0.15 -0.8 974,834 + BBR_RS14265 0.20 -0.1 974,841 + BBR_RS14265 0.20 -1.5 974,860 - BBR_RS14265 0.22 +0.1 974,893 - BBR_RS14265 0.25 +2.5 974,903 + BBR_RS14265 0.25 -0.3 974,915 - BBR_RS14265 0.27 +0.5 974,926 + BBR_RS14265 0.27 -0.5 975,051 + BBR_RS14265 0.38 -0.3 975,102 - BBR_RS14265 0.42 +0.9 975,102 - BBR_RS14265 0.42 +1.5 975,113 - BBR_RS14265 0.43 -0.3 975,151 + BBR_RS14265 0.47 -0.5 975,159 + BBR_RS14265 0.47 -0.9 975,191 - BBR_RS14265 0.50 -0.5 975,288 + BBR_RS14265 0.58 -1.2 975,295 + BBR_RS14265 0.59 -1.4 975,296 - BBR_RS14265 0.59 +0.5 975,324 + BBR_RS14265 0.61 -1.2 975,469 + BBR_RS14265 0.74 -1.9 975,486 + BBR_RS14265 0.75 +0.2 975,489 - BBR_RS14265 0.75 -0.9 975,489 - BBR_RS14265 0.75 -0.3 975,516 - BBR_RS14265 0.78 +2.5 975,611 + BBR_RS14265 0.86 +0.5 975,674 - +2.5 975,716 + -0.3 975,818 + -0.8 975,860 + -0.9 975,860 + -0.1 975,875 + +0.3 975,875 + -0.7 975,875 + +0.1 976,749 + -0.5 976,757 - -0.7 976,766 + -0.2
Or see this region's nucleotide sequence