Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14210 and BBR_RS14215 are separated by 21 nucleotides BBR_RS14215 and BBR_RS14220 are separated by 151 nucleotides
BBR_RS14210: BBR_RS14210 - hypothetical protein, at 963,532 to 965,073
_RS14210
BBR_RS14215: BBR_RS14215 - peptidase, at 965,095 to 965,457
_RS14215
BBR_RS14220: BBR_RS14220 - phosphomannomutase, at 965,609 to 966,412
_RS14220
Position (kb) 965
966 Strain fitness (log2 ratio) -2
-1
0
1
2 at 964.448 kb on - strand, within BBR_RS14210 at 964.526 kb on - strand, within BBR_RS14210 at 965.078 kb on - strand at 965.129 kb on - strand at 965.220 kb on - strand, within BBR_RS14215 at 965.232 kb on + strand, within BBR_RS14215 at 965.235 kb on - strand, within BBR_RS14215 at 965.240 kb on - strand, within BBR_RS14215 at 965.358 kb on + strand, within BBR_RS14215 at 965.358 kb on + strand, within BBR_RS14215 at 965.361 kb on - strand, within BBR_RS14215 at 965.454 kb on + strand at 965.471 kb on + strand at 965.472 kb on - strand at 965.479 kb on - strand at 965.503 kb on + strand at 965.570 kb on - strand at 965.589 kb on - strand at 965.620 kb on + strand at 965.673 kb on + strand at 965.678 kb on + strand at 965.681 kb on - strand at 965.700 kb on + strand, within BBR_RS14220 at 965.700 kb on + strand, within BBR_RS14220 at 965.703 kb on - strand, within BBR_RS14220 at 965.708 kb on - strand, within BBR_RS14220 at 965.710 kb on + strand, within BBR_RS14220 at 965.786 kb on + strand, within BBR_RS14220 at 965.810 kb on - strand, within BBR_RS14220 at 965.834 kb on - strand, within BBR_RS14220 at 965.862 kb on - strand, within BBR_RS14220 at 965.865 kb on + strand, within BBR_RS14220 at 965.883 kb on + strand, within BBR_RS14220 at 965.891 kb on + strand, within BBR_RS14220 at 965.897 kb on - strand, within BBR_RS14220 at 965.919 kb on - strand, within BBR_RS14220 at 965.961 kb on + strand, within BBR_RS14220 at 965.984 kb on - strand, within BBR_RS14220 at 966.096 kb on - strand, within BBR_RS14220 at 966.113 kb on - strand, within BBR_RS14220 at 966.215 kb on + strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.222 kb on - strand, within BBR_RS14220 at 966.234 kb on - strand, within BBR_RS14220 at 966.281 kb on + strand, within BBR_RS14220 at 966.299 kb on - strand, within BBR_RS14220 at 966.367 kb on + strand at 966.367 kb on + strand at 966.386 kb on - strand at 966.391 kb on + strand at 966.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 964,448 - BBR_RS14210 0.59 +1.6 964,526 - BBR_RS14210 0.64 +2.6 965,078 - -0.0 965,129 - -1.2 965,220 - BBR_RS14215 0.34 -1.0 965,232 + BBR_RS14215 0.38 -0.8 965,235 - BBR_RS14215 0.39 -0.0 965,240 - BBR_RS14215 0.40 -0.6 965,358 + BBR_RS14215 0.72 -1.5 965,358 + BBR_RS14215 0.72 -1.0 965,361 - BBR_RS14215 0.73 -0.8 965,454 + +0.3 965,471 + -1.3 965,472 - -1.9 965,479 - +0.2 965,503 + +0.1 965,570 - -0.0 965,589 - -1.9 965,620 + +0.9 965,673 + -0.3 965,678 + +0.2 965,681 - +0.7 965,700 + BBR_RS14220 0.11 +0.4 965,700 + BBR_RS14220 0.11 -1.4 965,703 - BBR_RS14220 0.12 +2.4 965,708 - BBR_RS14220 0.12 +1.0 965,710 + BBR_RS14220 0.13 +0.3 965,786 + BBR_RS14220 0.22 -0.0 965,810 - BBR_RS14220 0.25 +0.1 965,834 - BBR_RS14220 0.28 -1.2 965,862 - BBR_RS14220 0.31 +0.3 965,865 + BBR_RS14220 0.32 -0.0 965,883 + BBR_RS14220 0.34 +0.4 965,891 + BBR_RS14220 0.35 +0.6 965,897 - BBR_RS14220 0.36 -0.1 965,919 - BBR_RS14220 0.39 -1.7 965,961 + BBR_RS14220 0.44 -0.1 965,984 - BBR_RS14220 0.47 -0.6 966,096 - BBR_RS14220 0.61 -0.6 966,113 - BBR_RS14220 0.63 +0.4 966,215 + BBR_RS14220 0.75 +0.9 966,222 - BBR_RS14220 0.76 -0.5 966,222 - BBR_RS14220 0.76 +0.8 966,222 - BBR_RS14220 0.76 +0.8 966,234 - BBR_RS14220 0.78 +0.5 966,281 + BBR_RS14220 0.84 -0.8 966,299 - BBR_RS14220 0.86 +0.6 966,367 + -0.5 966,367 + +0.7 966,386 - -0.5 966,391 + +0.5 966,409 - -0.3
Or see this region's nucleotide sequence