Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14205 and BBR_RS14210 overlap by 4 nucleotides BBR_RS14210 and BBR_RS14215 are separated by 21 nucleotides BBR_RS14215 and BBR_RS14220 are separated by 151 nucleotides
BBR_RS14205: BBR_RS14205 - hypothetical protein, at 960,443 to 963,535
_RS14205
BBR_RS14210: BBR_RS14210 - hypothetical protein, at 963,532 to 965,073
_RS14210
BBR_RS14215: BBR_RS14215 - peptidase, at 965,095 to 965,457
_RS14215
BBR_RS14220: BBR_RS14220 - phosphomannomutase, at 965,609 to 966,412
_RS14220
Position (kb) 963
964
965
966 Strain fitness (log2 ratio) -2
-1
0
1
2 at 962.742 kb on - strand, within BBR_RS14205 at 962.794 kb on + strand, within BBR_RS14205 at 962.802 kb on - strand, within BBR_RS14205 at 962.827 kb on + strand, within BBR_RS14205 at 962.830 kb on + strand, within BBR_RS14205 at 962.836 kb on + strand, within BBR_RS14205 at 962.946 kb on + strand, within BBR_RS14205 at 963.245 kb on + strand at 963.275 kb on - strand at 963.383 kb on + strand at 963.391 kb on + strand at 963.391 kb on + strand at 963.392 kb on + strand at 963.399 kb on - strand at 963.414 kb on + strand at 963.422 kb on - strand at 963.456 kb on - strand at 963.483 kb on - strand at 963.495 kb on + strand at 963.516 kb on + strand at 963.555 kb on + strand at 963.709 kb on - strand, within BBR_RS14210 at 963.993 kb on - strand, within BBR_RS14210 at 964.075 kb on + strand, within BBR_RS14210 at 964.448 kb on - strand, within BBR_RS14210 at 964.526 kb on - strand, within BBR_RS14210 at 965.078 kb on - strand at 965.129 kb on - strand at 965.220 kb on - strand, within BBR_RS14215 at 965.232 kb on + strand, within BBR_RS14215 at 965.235 kb on - strand, within BBR_RS14215 at 965.240 kb on - strand, within BBR_RS14215 at 965.358 kb on + strand, within BBR_RS14215 at 965.358 kb on + strand, within BBR_RS14215 at 965.361 kb on - strand, within BBR_RS14215 at 965.454 kb on + strand at 965.471 kb on + strand at 965.472 kb on - strand at 965.479 kb on - strand at 965.503 kb on + strand at 965.570 kb on - strand at 965.589 kb on - strand at 965.620 kb on + strand at 965.673 kb on + strand at 965.678 kb on + strand at 965.681 kb on - strand at 965.700 kb on + strand, within BBR_RS14220 at 965.700 kb on + strand, within BBR_RS14220 at 965.703 kb on - strand, within BBR_RS14220 at 965.708 kb on - strand, within BBR_RS14220 at 965.710 kb on + strand, within BBR_RS14220 at 965.786 kb on + strand, within BBR_RS14220 at 965.810 kb on - strand, within BBR_RS14220 at 965.834 kb on - strand, within BBR_RS14220 at 965.862 kb on - strand, within BBR_RS14220 at 965.865 kb on + strand, within BBR_RS14220 at 965.883 kb on + strand, within BBR_RS14220 at 965.891 kb on + strand, within BBR_RS14220 at 965.897 kb on - strand, within BBR_RS14220 at 965.919 kb on - strand, within BBR_RS14220 at 965.961 kb on + strand, within BBR_RS14220 at 965.984 kb on - strand, within BBR_RS14220
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 962,742 - BBR_RS14205 0.74 +1.0 962,794 + BBR_RS14205 0.76 -0.6 962,802 - BBR_RS14205 0.76 +2.6 962,827 + BBR_RS14205 0.77 -2.4 962,830 + BBR_RS14205 0.77 -2.0 962,836 + BBR_RS14205 0.77 +0.2 962,946 + BBR_RS14205 0.81 +2.6 963,245 + +0.2 963,275 - +1.0 963,383 + -0.7 963,391 + -1.8 963,391 + +0.1 963,392 + -1.0 963,399 - -1.5 963,414 + -0.1 963,422 - +0.3 963,456 - +0.6 963,483 - +0.3 963,495 + +0.3 963,516 + +0.4 963,555 + +0.6 963,709 - BBR_RS14210 0.11 +2.6 963,993 - BBR_RS14210 0.30 +2.6 964,075 + BBR_RS14210 0.35 +0.2 964,448 - BBR_RS14210 0.59 +1.6 964,526 - BBR_RS14210 0.64 +2.6 965,078 - -0.0 965,129 - -1.2 965,220 - BBR_RS14215 0.34 -1.0 965,232 + BBR_RS14215 0.38 -0.8 965,235 - BBR_RS14215 0.39 -0.0 965,240 - BBR_RS14215 0.40 -0.6 965,358 + BBR_RS14215 0.72 -1.5 965,358 + BBR_RS14215 0.72 -1.0 965,361 - BBR_RS14215 0.73 -0.8 965,454 + +0.3 965,471 + -1.3 965,472 - -1.9 965,479 - +0.2 965,503 + +0.1 965,570 - -0.0 965,589 - -1.9 965,620 + +0.9 965,673 + -0.3 965,678 + +0.2 965,681 - +0.7 965,700 + BBR_RS14220 0.11 +0.4 965,700 + BBR_RS14220 0.11 -1.4 965,703 - BBR_RS14220 0.12 +2.4 965,708 - BBR_RS14220 0.12 +1.0 965,710 + BBR_RS14220 0.13 +0.3 965,786 + BBR_RS14220 0.22 -0.0 965,810 - BBR_RS14220 0.25 +0.1 965,834 - BBR_RS14220 0.28 -1.2 965,862 - BBR_RS14220 0.31 +0.3 965,865 + BBR_RS14220 0.32 -0.0 965,883 + BBR_RS14220 0.34 +0.4 965,891 + BBR_RS14220 0.35 +0.6 965,897 - BBR_RS14220 0.36 -0.1 965,919 - BBR_RS14220 0.39 -1.7 965,961 + BBR_RS14220 0.44 -0.1 965,984 - BBR_RS14220 0.47 -0.6
Or see this region's nucleotide sequence