Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14115 and BBR_RS14120 overlap by 4 nucleotides BBR_RS14120 and BBR_RS14125 are separated by 150 nucleotides
BBR_RS14115: BBR_RS14115 - transcription-repair coupling factor, at 941,761 to 945,351
_RS14115
BBR_RS14120: BBR_RS14120 - aldo/keto reductase, at 945,348 to 946,259
_RS14120
BBR_RS14125: BBR_RS14125 - phosphopyruvate hydratase, at 946,410 to 947,708
_RS14125
Position (kb) 945
946
947 Strain fitness (log2 ratio) -1
0
1
2 at 944.354 kb on - strand, within BBR_RS14115 at 944.354 kb on - strand, within BBR_RS14115 at 944.416 kb on + strand, within BBR_RS14115 at 944.433 kb on + strand, within BBR_RS14115 at 944.488 kb on + strand, within BBR_RS14115 at 944.637 kb on - strand, within BBR_RS14115 at 944.652 kb on - strand, within BBR_RS14115 at 944.655 kb on + strand, within BBR_RS14115 at 944.701 kb on + strand, within BBR_RS14115 at 944.701 kb on + strand, within BBR_RS14115 at 944.755 kb on - strand, within BBR_RS14115 at 944.799 kb on + strand, within BBR_RS14115 at 944.821 kb on + strand, within BBR_RS14115 at 945.069 kb on + strand at 945.097 kb on - strand at 945.132 kb on + strand at 945.140 kb on - strand at 945.163 kb on - strand at 945.223 kb on + strand at 945.274 kb on + strand at 945.276 kb on - strand at 945.293 kb on + strand at 945.305 kb on + strand at 945.364 kb on + strand at 945.456 kb on + strand, within BBR_RS14120 at 945.464 kb on - strand, within BBR_RS14120 at 945.472 kb on - strand, within BBR_RS14120 at 945.474 kb on + strand, within BBR_RS14120 at 945.474 kb on + strand, within BBR_RS14120 at 945.479 kb on + strand, within BBR_RS14120 at 945.546 kb on + strand, within BBR_RS14120 at 945.614 kb on + strand, within BBR_RS14120 at 945.632 kb on + strand, within BBR_RS14120 at 945.657 kb on + strand, within BBR_RS14120 at 945.657 kb on + strand, within BBR_RS14120 at 945.665 kb on - strand, within BBR_RS14120 at 945.737 kb on + strand, within BBR_RS14120 at 945.782 kb on - strand, within BBR_RS14120 at 945.797 kb on - strand, within BBR_RS14120 at 945.797 kb on - strand, within BBR_RS14120 at 945.812 kb on - strand, within BBR_RS14120 at 945.840 kb on + strand, within BBR_RS14120 at 945.845 kb on + strand, within BBR_RS14120 at 945.845 kb on + strand, within BBR_RS14120 at 945.853 kb on - strand, within BBR_RS14120 at 945.943 kb on + strand, within BBR_RS14120 at 945.953 kb on + strand, within BBR_RS14120 at 945.979 kb on + strand, within BBR_RS14120 at 945.979 kb on + strand, within BBR_RS14120 at 945.979 kb on + strand, within BBR_RS14120 at 945.987 kb on - strand, within BBR_RS14120 at 945.989 kb on + strand, within BBR_RS14120 at 946.101 kb on - strand, within BBR_RS14120 at 946.101 kb on - strand, within BBR_RS14120 at 946.101 kb on - strand, within BBR_RS14120 at 946.125 kb on - strand, within BBR_RS14120 at 946.131 kb on - strand, within BBR_RS14120 at 946.268 kb on + strand at 946.337 kb on + strand at 946.345 kb on - strand at 946.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 944,354 - BBR_RS14115 0.72 -0.1 944,354 - BBR_RS14115 0.72 +0.3 944,416 + BBR_RS14115 0.74 +0.2 944,433 + BBR_RS14115 0.74 -0.7 944,488 + BBR_RS14115 0.76 +1.0 944,637 - BBR_RS14115 0.80 -0.4 944,652 - BBR_RS14115 0.81 +0.6 944,655 + BBR_RS14115 0.81 +0.7 944,701 + BBR_RS14115 0.82 -1.4 944,701 + BBR_RS14115 0.82 +0.4 944,755 - BBR_RS14115 0.83 +0.6 944,799 + BBR_RS14115 0.85 -0.8 944,821 + BBR_RS14115 0.85 +0.7 945,069 + -0.5 945,097 - -0.6 945,132 + -0.1 945,140 - +0.9 945,163 - -0.6 945,223 + +0.2 945,274 + -0.1 945,276 - -0.1 945,293 + -1.4 945,305 + +0.2 945,364 + -0.2 945,456 + BBR_RS14120 0.12 -0.1 945,464 - BBR_RS14120 0.13 -0.3 945,472 - BBR_RS14120 0.14 +0.5 945,474 + BBR_RS14120 0.14 -0.2 945,474 + BBR_RS14120 0.14 +0.5 945,479 + BBR_RS14120 0.14 -0.2 945,546 + BBR_RS14120 0.22 -1.2 945,614 + BBR_RS14120 0.29 -0.0 945,632 + BBR_RS14120 0.31 +0.9 945,657 + BBR_RS14120 0.34 +0.1 945,657 + BBR_RS14120 0.34 -0.0 945,665 - BBR_RS14120 0.35 -0.1 945,737 + BBR_RS14120 0.43 +0.6 945,782 - BBR_RS14120 0.48 +1.2 945,797 - BBR_RS14120 0.49 +1.2 945,797 - BBR_RS14120 0.49 -1.0 945,812 - BBR_RS14120 0.51 -1.4 945,840 + BBR_RS14120 0.54 -0.4 945,845 + BBR_RS14120 0.54 +0.3 945,845 + BBR_RS14120 0.54 -0.7 945,853 - BBR_RS14120 0.55 +0.8 945,943 + BBR_RS14120 0.65 +0.6 945,953 + BBR_RS14120 0.66 +0.6 945,979 + BBR_RS14120 0.69 -0.4 945,979 + BBR_RS14120 0.69 -0.0 945,979 + BBR_RS14120 0.69 -0.3 945,987 - BBR_RS14120 0.70 +1.8 945,989 + BBR_RS14120 0.70 -1.1 946,101 - BBR_RS14120 0.83 -0.6 946,101 - BBR_RS14120 0.83 +0.4 946,101 - BBR_RS14120 0.83 +0.5 946,125 - BBR_RS14120 0.85 -0.2 946,131 - BBR_RS14120 0.86 +0.3 946,268 + +0.1 946,337 + -0.1 946,345 - +0.2 946,346 - +0.4
Or see this region's nucleotide sequence