Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14035 and BBR_RS14040 are separated by 123 nucleotides BBR_RS14040 and BBR_RS14045 are separated by 289 nucleotides BBR_RS14045 and BBR_RS14050 are separated by 21 nucleotides BBR_RS14050 and BBR_RS14055 are separated by 6 nucleotides
BBR_RS14035: BBR_RS14035 - HAD family phosphatase, at 925,877 to 926,524
_RS14035
BBR_RS14040: BBR_RS14040 - GNAT family N-acetyltransferase, at 926,648 to 927,123
_RS14040
BBR_RS14045: BBR_RS14045 - GntR family transcriptional regulator, at 927,413 to 927,787
_RS14045
BBR_RS14050: BBR_RS14050 - ABC transporter ATP-binding protein, at 927,809 to 928,729
_RS14050
BBR_RS14055: BBR_RS14055 - ABC-2 transporter permease, at 928,736 to 929,449
_RS14055
Position (kb) 927
928 Strain fitness (log2 ratio) -2
-1
0
1
2 at 926.419 kb on + strand, within BBR_RS14035 at 926.420 kb on - strand, within BBR_RS14035 at 926.420 kb on - strand, within BBR_RS14035 at 926.424 kb on + strand, within BBR_RS14035 at 926.427 kb on - strand, within BBR_RS14035 at 926.455 kb on - strand, within BBR_RS14035 at 926.508 kb on + strand at 926.532 kb on + strand at 926.541 kb on + strand at 926.619 kb on + strand at 926.639 kb on + strand at 926.642 kb on - strand at 926.664 kb on - strand at 926.731 kb on + strand, within BBR_RS14040 at 926.814 kb on - strand, within BBR_RS14040 at 926.814 kb on - strand, within BBR_RS14040 at 926.852 kb on + strand, within BBR_RS14040 at 926.977 kb on + strand, within BBR_RS14040 at 926.987 kb on + strand, within BBR_RS14040 at 926.987 kb on + strand, within BBR_RS14040 at 926.992 kb on + strand, within BBR_RS14040 at 926.995 kb on - strand, within BBR_RS14040 at 927.019 kb on + strand, within BBR_RS14040 at 927.031 kb on + strand, within BBR_RS14040 at 927.058 kb on + strand, within BBR_RS14040 at 927.058 kb on + strand, within BBR_RS14040 at 927.192 kb on + strand at 927.197 kb on + strand at 927.223 kb on - strand at 927.229 kb on + strand at 927.237 kb on - strand at 927.237 kb on - strand at 927.412 kb on + strand at 927.437 kb on - strand at 927.438 kb on + strand at 927.487 kb on + strand, within BBR_RS14045 at 927.555 kb on + strand, within BBR_RS14045 at 927.555 kb on + strand, within BBR_RS14045 at 927.608 kb on + strand, within BBR_RS14045 at 927.673 kb on + strand, within BBR_RS14045 at 927.673 kb on + strand, within BBR_RS14045 at 927.746 kb on + strand, within BBR_RS14045 at 927.746 kb on + strand, within BBR_RS14045 at 927.747 kb on + strand, within BBR_RS14045 at 927.747 kb on + strand, within BBR_RS14045 at 927.747 kb on + strand, within BBR_RS14045 at 927.781 kb on + strand at 927.858 kb on + strand at 927.868 kb on + strand at 927.899 kb on + strand at 927.909 kb on + strand, within BBR_RS14050 at 927.924 kb on - strand, within BBR_RS14050 at 927.957 kb on + strand, within BBR_RS14050 at 927.967 kb on + strand, within BBR_RS14050 at 927.967 kb on + strand, within BBR_RS14050 at 927.984 kb on + strand, within BBR_RS14050 at 928.033 kb on - strand, within BBR_RS14050 at 928.082 kb on - strand, within BBR_RS14050 at 928.102 kb on - strand, within BBR_RS14050 at 928.109 kb on + strand, within BBR_RS14050 at 928.131 kb on - strand, within BBR_RS14050 at 928.146 kb on - strand, within BBR_RS14050 at 928.164 kb on + strand, within BBR_RS14050 at 928.185 kb on + strand, within BBR_RS14050 at 928.185 kb on + strand, within BBR_RS14050 at 928.185 kb on + strand, within BBR_RS14050 at 928.193 kb on - strand, within BBR_RS14050 at 928.298 kb on + strand, within BBR_RS14050 at 928.328 kb on - strand, within BBR_RS14050 at 928.376 kb on - strand, within BBR_RS14050 at 928.386 kb on + strand, within BBR_RS14050 at 928.386 kb on + strand, within BBR_RS14050 at 928.396 kb on + strand, within BBR_RS14050 at 928.396 kb on + strand, within BBR_RS14050 at 928.404 kb on - strand, within BBR_RS14050 at 928.448 kb on - strand, within BBR_RS14050 at 928.468 kb on + strand, within BBR_RS14050 at 928.468 kb on + strand, within BBR_RS14050 at 928.476 kb on - strand, within BBR_RS14050 at 928.513 kb on + strand, within BBR_RS14050 at 928.566 kb on + strand, within BBR_RS14050 at 928.569 kb on + strand, within BBR_RS14050 at 928.605 kb on + strand, within BBR_RS14050 at 928.614 kb on + strand, within BBR_RS14050 at 928.665 kb on + strand at 928.753 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 926,419 + BBR_RS14035 0.84 -0.6 926,420 - BBR_RS14035 0.84 +1.8 926,420 - BBR_RS14035 0.84 -2.3 926,424 + BBR_RS14035 0.84 +0.1 926,427 - BBR_RS14035 0.85 -1.0 926,455 - BBR_RS14035 0.89 +0.5 926,508 + +0.3 926,532 + +0.5 926,541 + +0.7 926,619 + -0.2 926,639 + +1.0 926,642 - +0.8 926,664 - +0.2 926,731 + BBR_RS14040 0.17 +1.0 926,814 - BBR_RS14040 0.35 -0.2 926,814 - BBR_RS14040 0.35 +0.9 926,852 + BBR_RS14040 0.43 -1.4 926,977 + BBR_RS14040 0.69 -0.6 926,987 + BBR_RS14040 0.71 -0.9 926,987 + BBR_RS14040 0.71 -0.0 926,992 + BBR_RS14040 0.72 -0.2 926,995 - BBR_RS14040 0.73 +0.0 927,019 + BBR_RS14040 0.78 -0.4 927,031 + BBR_RS14040 0.80 +0.4 927,058 + BBR_RS14040 0.86 -0.2 927,058 + BBR_RS14040 0.86 +0.6 927,192 + -1.4 927,197 + +0.2 927,223 - +0.0 927,229 + +0.2 927,237 - +0.3 927,237 - -1.9 927,412 + -1.1 927,437 - +1.0 927,438 + -1.5 927,487 + BBR_RS14045 0.20 -1.0 927,555 + BBR_RS14045 0.38 -1.0 927,555 + BBR_RS14045 0.38 -0.6 927,608 + BBR_RS14045 0.52 -1.3 927,673 + BBR_RS14045 0.69 -0.2 927,673 + BBR_RS14045 0.69 -0.5 927,746 + BBR_RS14045 0.89 -0.2 927,746 + BBR_RS14045 0.89 -0.8 927,747 + BBR_RS14045 0.89 -1.3 927,747 + BBR_RS14045 0.89 -0.7 927,747 + BBR_RS14045 0.89 -1.5 927,781 + +2.0 927,858 + -0.2 927,868 + +0.1 927,899 + -1.0 927,909 + BBR_RS14050 0.11 +0.1 927,924 - BBR_RS14050 0.12 -0.1 927,957 + BBR_RS14050 0.16 +0.1 927,967 + BBR_RS14050 0.17 +1.0 927,967 + BBR_RS14050 0.17 +0.4 927,984 + BBR_RS14050 0.19 +0.2 928,033 - BBR_RS14050 0.24 +0.6 928,082 - BBR_RS14050 0.30 +0.1 928,102 - BBR_RS14050 0.32 +1.3 928,109 + BBR_RS14050 0.33 +1.2 928,131 - BBR_RS14050 0.35 -1.7 928,146 - BBR_RS14050 0.37 -0.7 928,164 + BBR_RS14050 0.39 +0.5 928,185 + BBR_RS14050 0.41 -0.3 928,185 + BBR_RS14050 0.41 +1.6 928,185 + BBR_RS14050 0.41 -0.8 928,193 - BBR_RS14050 0.42 +0.9 928,298 + BBR_RS14050 0.53 -1.4 928,328 - BBR_RS14050 0.56 -1.1 928,376 - BBR_RS14050 0.62 -0.3 928,386 + BBR_RS14050 0.63 -1.3 928,386 + BBR_RS14050 0.63 +0.4 928,396 + BBR_RS14050 0.64 +1.3 928,396 + BBR_RS14050 0.64 +0.3 928,404 - BBR_RS14050 0.65 -0.2 928,448 - BBR_RS14050 0.69 -2.0 928,468 + BBR_RS14050 0.72 -0.2 928,468 + BBR_RS14050 0.72 -0.0 928,476 - BBR_RS14050 0.72 -0.1 928,513 + BBR_RS14050 0.76 +0.9 928,566 + BBR_RS14050 0.82 -0.5 928,569 + BBR_RS14050 0.83 +0.8 928,605 + BBR_RS14050 0.86 +0.2 928,614 + BBR_RS14050 0.87 -0.5 928,665 + -0.8 928,753 - -0.3
Or see this region's nucleotide sequence