Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13970 and BBR_RS13975 are separated by 144 nucleotides BBR_RS13975 and BBR_RS13980 are separated by 197 nucleotides BBR_RS13980 and BBR_RS13985 are separated by 9 nucleotides BBR_RS13985 and BBR_RS13990 are separated by 20 nucleotides
BBR_RS13970: BBR_RS13970 - thiazole biosynthesis adenylyltransferase ThiF, at 911,095 to 911,904
_RS13970
BBR_RS13975: BBR_RS13975 - rhodanese-like domain-containing protein, at 912,049 to 912,441
_RS13975
BBR_RS13980: BBR_RS13980 - HD domain-containing protein, at 912,639 to 913,208
_RS13980
BBR_RS13985: BBR_RS13985 - membrane protein, at 913,218 to 913,985
_RS13985
BBR_RS13990: BBR_RS13990 - DUF975 domain-containing protein, at 914,006 to 915,826
_RS13990
Position (kb)
912
913
914 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 911.685 kb on - strand, within BBR_RS13970 at 911.699 kb on + strand, within BBR_RS13970 at 911.701 kb on + strand, within BBR_RS13970 at 911.707 kb on - strand, within BBR_RS13970 at 911.731 kb on - strand, within BBR_RS13970 at 911.764 kb on - strand, within BBR_RS13970 at 911.776 kb on - strand, within BBR_RS13970 at 911.776 kb on - strand, within BBR_RS13970 at 911.836 kb on - strand at 911.840 kb on - strand at 911.841 kb on - strand at 911.842 kb on - strand at 911.845 kb on - strand at 911.899 kb on + strand at 911.899 kb on + strand at 911.907 kb on - strand at 911.907 kb on - strand at 911.917 kb on + strand at 911.928 kb on - strand at 911.941 kb on + strand at 911.941 kb on + strand at 911.954 kb on - strand at 912.009 kb on + strand at 912.011 kb on + strand at 912.011 kb on + strand at 912.011 kb on + strand at 912.029 kb on - strand at 912.029 kb on - strand at 912.029 kb on - strand at 912.039 kb on - strand at 912.039 kb on - strand at 912.058 kb on + strand at 912.090 kb on + strand, within BBR_RS13975 at 912.104 kb on - strand, within BBR_RS13975 at 912.149 kb on + strand, within BBR_RS13975 at 912.157 kb on - strand, within BBR_RS13975 at 912.199 kb on + strand, within BBR_RS13975 at 912.199 kb on + strand, within BBR_RS13975 at 912.246 kb on - strand, within BBR_RS13975 at 912.251 kb on - strand, within BBR_RS13975 at 912.271 kb on + strand, within BBR_RS13975 at 912.276 kb on + strand, within BBR_RS13975 at 912.279 kb on - strand, within BBR_RS13975 at 912.310 kb on - strand, within BBR_RS13975 at 912.359 kb on - strand, within BBR_RS13975 at 912.359 kb on - strand, within BBR_RS13975 at 912.359 kb on - strand, within BBR_RS13975 at 912.362 kb on - strand, within BBR_RS13975 at 912.363 kb on + strand, within BBR_RS13975 at 912.371 kb on + strand, within BBR_RS13975 at 912.372 kb on - strand, within BBR_RS13975 at 912.430 kb on + strand at 912.430 kb on + strand at 912.435 kb on + strand at 912.443 kb on - strand at 912.457 kb on - strand at 912.488 kb on + strand at 912.488 kb on + strand at 912.488 kb on + strand at 912.493 kb on + strand at 912.517 kb on + strand at 912.555 kb on + strand at 912.560 kb on + strand at 912.563 kb on + strand at 912.570 kb on - strand at 912.637 kb on + strand at 912.691 kb on + strand at 912.699 kb on - strand, within BBR_RS13980 at 912.808 kb on + strand, within BBR_RS13980 at 912.897 kb on + strand, within BBR_RS13980 at 912.897 kb on + strand, within BBR_RS13980 at 912.915 kb on - strand, within BBR_RS13980 at 912.940 kb on + strand, within BBR_RS13980 at 912.966 kb on + strand, within BBR_RS13980 at 913.060 kb on + strand, within BBR_RS13980 at 913.087 kb on - strand, within BBR_RS13980 at 913.161 kb on + strand at 913.179 kb on - strand at 913.184 kb on + strand at 913.262 kb on - strand at 913.334 kb on + strand, within BBR_RS13985 at 913.377 kb on - strand, within BBR_RS13985 at 913.444 kb on - strand, within BBR_RS13985 at 913.487 kb on + strand, within BBR_RS13985 at 913.538 kb on - strand, within BBR_RS13985 at 913.628 kb on + strand, within BBR_RS13985 at 913.755 kb on + strand, within BBR_RS13985 at 913.755 kb on + strand, within BBR_RS13985 at 913.768 kb on - strand, within BBR_RS13985 at 913.774 kb on - strand, within BBR_RS13985 at 913.774 kb on - strand, within BBR_RS13985 at 913.783 kb on + strand, within BBR_RS13985 at 913.864 kb on - strand, within BBR_RS13985 at 913.864 kb on - strand, within BBR_RS13985 at 913.865 kb on + strand, within BBR_RS13985 at 913.894 kb on + strand, within BBR_RS13985 at 913.901 kb on + strand, within BBR_RS13985 at 913.909 kb on - strand at 914.024 kb on + strand at 914.065 kb on - strand at 914.092 kb on - strand at 914.113 kb on - strand at 914.117 kb on + strand at 914.125 kb on - strand at 914.141 kb on - strand at 914.182 kb on + strand at 914.186 kb on - strand at 914.191 kb on - strand, within BBR_RS13990 at 914.200 kb on - strand, within BBR_RS13990
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 911,685 - BBR_RS13970 0.73 +0.3 911,699 + BBR_RS13970 0.75 -0.4 911,701 + BBR_RS13970 0.75 -0.7 911,707 - BBR_RS13970 0.76 -1.6 911,731 - BBR_RS13970 0.79 -0.3 911,764 - BBR_RS13970 0.83 -0.0 911,776 - BBR_RS13970 0.84 -0.1 911,776 - BBR_RS13970 0.84 +0.9 911,836 - +0.8 911,840 - +0.7 911,841 - +1.1 911,842 - -0.0 911,845 - -0.1 911,899 + -0.6 911,899 + -0.0 911,907 - -2.3 911,907 - +1.6 911,917 + -0.5 911,928 - +2.5 911,941 + +0.5 911,941 + -0.5 911,954 - -0.3 912,009 + +0.1 912,011 + +0.9 912,011 + +0.2 912,011 + +0.4 912,029 - +0.2 912,029 - +0.4 912,029 - +0.5 912,039 - +0.2 912,039 - -0.3 912,058 + +0.2 912,090 + BBR_RS13975 0.10 +0.4 912,104 - BBR_RS13975 0.14 -0.1 912,149 + BBR_RS13975 0.25 +1.4 912,157 - BBR_RS13975 0.27 +0.7 912,199 + BBR_RS13975 0.38 -0.9 912,199 + BBR_RS13975 0.38 -0.1 912,246 - BBR_RS13975 0.50 -0.1 912,251 - BBR_RS13975 0.51 +0.9 912,271 + BBR_RS13975 0.56 -0.4 912,276 + BBR_RS13975 0.58 -0.1 912,279 - BBR_RS13975 0.59 -0.5 912,310 - BBR_RS13975 0.66 -0.2 912,359 - BBR_RS13975 0.79 -0.2 912,359 - BBR_RS13975 0.79 -0.1 912,359 - BBR_RS13975 0.79 -0.1 912,362 - BBR_RS13975 0.80 -1.2 912,363 + BBR_RS13975 0.80 -1.0 912,371 + BBR_RS13975 0.82 +0.6 912,372 - BBR_RS13975 0.82 -0.5 912,430 + -0.1 912,430 + +0.4 912,435 + -0.8 912,443 - +0.3 912,457 - -0.8 912,488 + +1.1 912,488 + +0.1 912,488 + +0.0 912,493 + +0.3 912,517 + -0.1 912,555 + +0.7 912,560 + +0.2 912,563 + +0.1 912,570 - +0.7 912,637 + +0.5 912,691 + -0.6 912,699 - BBR_RS13980 0.11 -0.2 912,808 + BBR_RS13980 0.30 -0.5 912,897 + BBR_RS13980 0.45 -0.5 912,897 + BBR_RS13980 0.45 -1.2 912,915 - BBR_RS13980 0.48 +0.2 912,940 + BBR_RS13980 0.53 -0.7 912,966 + BBR_RS13980 0.57 -1.2 913,060 + BBR_RS13980 0.74 -0.8 913,087 - BBR_RS13980 0.79 -0.3 913,161 + -1.1 913,179 - +0.4 913,184 + -0.6 913,262 - +1.3 913,334 + BBR_RS13985 0.15 -0.1 913,377 - BBR_RS13985 0.21 -0.8 913,444 - BBR_RS13985 0.29 +0.1 913,487 + BBR_RS13985 0.35 -0.2 913,538 - BBR_RS13985 0.42 +1.6 913,628 + BBR_RS13985 0.53 -3.4 913,755 + BBR_RS13985 0.70 -0.0 913,755 + BBR_RS13985 0.70 -0.4 913,768 - BBR_RS13985 0.72 -0.7 913,774 - BBR_RS13985 0.72 -0.9 913,774 - BBR_RS13985 0.72 -0.2 913,783 + BBR_RS13985 0.74 +0.5 913,864 - BBR_RS13985 0.84 +0.3 913,864 - BBR_RS13985 0.84 -0.1 913,865 + BBR_RS13985 0.84 -0.6 913,894 + BBR_RS13985 0.88 +0.2 913,901 + BBR_RS13985 0.89 -1.1 913,909 - +0.6 914,024 + +0.2 914,065 - +0.6 914,092 - -0.7 914,113 - +0.4 914,117 + +0.4 914,125 - +2.6 914,141 - -0.8 914,182 + -0.8 914,186 - -0.4 914,191 - BBR_RS13990 0.10 -0.4 914,200 - BBR_RS13990 0.11 -0.6
Or see this region's nucleotide sequence