Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13900 and BBR_RS13905 are separated by 28 nucleotides BBR_RS13905 and BBR_RS13910 overlap by 4 nucleotides BBR_RS13910 and BBR_RS13915 are separated by 155 nucleotides
BBR_RS13900: BBR_RS13900 - acetylornithine transaminase, at 898,346 to 899,641
_RS13900
BBR_RS13905: BBR_RS13905 - ornithine carbamoyltransferase, at 899,670 to 900,635
_RS13905
BBR_RS13910: BBR_RS13910 - arginine repressor, at 900,632 to 901,144
_RS13910
BBR_RS13915: BBR_RS13915 - argininosuccinate synthase, at 901,300 to 902,538
_RS13915
Position (kb)
900
901
902 Strain fitness (log2 ratio)
-1
0
1
2
3 at 899.672 kb on + strand at 899.674 kb on + strand at 899.674 kb on + strand at 899.674 kb on + strand at 899.674 kb on + strand at 899.748 kb on + strand at 899.925 kb on + strand, within BBR_RS13905 at 899.930 kb on + strand, within BBR_RS13905 at 899.934 kb on + strand, within BBR_RS13905 at 899.934 kb on + strand, within BBR_RS13905 at 900.017 kb on + strand, within BBR_RS13905 at 900.073 kb on + strand, within BBR_RS13905 at 900.089 kb on + strand, within BBR_RS13905 at 900.145 kb on + strand, within BBR_RS13905 at 900.248 kb on + strand, within BBR_RS13905 at 900.298 kb on + strand, within BBR_RS13905 at 900.310 kb on + strand, within BBR_RS13905 at 900.310 kb on + strand, within BBR_RS13905 at 900.410 kb on + strand, within BBR_RS13905 at 900.418 kb on - strand, within BBR_RS13905 at 900.425 kb on + strand, within BBR_RS13905 at 900.425 kb on + strand, within BBR_RS13905 at 900.539 kb on + strand at 900.540 kb on + strand at 900.826 kb on + strand, within BBR_RS13910 at 900.932 kb on + strand, within BBR_RS13910 at 900.985 kb on + strand, within BBR_RS13910 at 901.159 kb on + strand at 901.167 kb on - strand at 901.171 kb on + strand at 901.179 kb on - strand at 901.190 kb on - strand at 901.205 kb on + strand at 901.213 kb on - strand at 901.316 kb on + strand at 901.326 kb on + strand at 901.329 kb on - strand at 901.339 kb on + strand at 901.339 kb on - strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.441 kb on - strand, within BBR_RS13915 at 901.442 kb on + strand, within BBR_RS13915 at 901.442 kb on + strand, within BBR_RS13915 at 901.442 kb on - strand, within BBR_RS13915 at 901.489 kb on + strand, within BBR_RS13915 at 901.539 kb on + strand, within BBR_RS13915 at 901.539 kb on + strand, within BBR_RS13915 at 901.567 kb on + strand, within BBR_RS13915 at 901.573 kb on - strand, within BBR_RS13915 at 901.606 kb on + strand, within BBR_RS13915 at 901.629 kb on - strand, within BBR_RS13915 at 901.676 kb on - strand, within BBR_RS13915 at 901.693 kb on + strand, within BBR_RS13915 at 901.693 kb on - strand, within BBR_RS13915 at 901.719 kb on - strand, within BBR_RS13915 at 901.728 kb on - strand, within BBR_RS13915 at 901.750 kb on + strand, within BBR_RS13915 at 901.774 kb on + strand, within BBR_RS13915 at 901.799 kb on + strand, within BBR_RS13915 at 901.809 kb on + strand, within BBR_RS13915 at 901.839 kb on + strand, within BBR_RS13915 at 901.851 kb on - strand, within BBR_RS13915 at 901.905 kb on + strand, within BBR_RS13915 at 901.905 kb on + strand, within BBR_RS13915 at 901.911 kb on + strand, within BBR_RS13915 at 901.919 kb on - strand, within BBR_RS13915 at 901.940 kb on - strand, within BBR_RS13915 at 901.998 kb on - strand, within BBR_RS13915 at 902.019 kb on - strand, within BBR_RS13915 at 902.021 kb on + strand, within BBR_RS13915 at 902.094 kb on - strand, within BBR_RS13915 at 902.105 kb on + strand, within BBR_RS13915 at 902.115 kb on + strand, within BBR_RS13915 at 902.122 kb on + strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.132 kb on + strand, within BBR_RS13915 at 902.137 kb on + strand, within BBR_RS13915 at 902.142 kb on + strand, within BBR_RS13915 at 902.142 kb on + strand, within BBR_RS13915
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 899,672 + +0.7 899,674 + -0.7 899,674 + +0.4 899,674 + -0.6 899,674 + +0.3 899,748 + -0.8 899,925 + BBR_RS13905 0.26 +1.7 899,930 + BBR_RS13905 0.27 +0.5 899,934 + BBR_RS13905 0.27 -0.5 899,934 + BBR_RS13905 0.27 -1.3 900,017 + BBR_RS13905 0.36 +0.3 900,073 + BBR_RS13905 0.42 +1.0 900,089 + BBR_RS13905 0.43 +0.3 900,145 + BBR_RS13905 0.49 -0.8 900,248 + BBR_RS13905 0.60 +0.2 900,298 + BBR_RS13905 0.65 +1.0 900,310 + BBR_RS13905 0.66 -0.5 900,310 + BBR_RS13905 0.66 -0.2 900,410 + BBR_RS13905 0.77 -0.1 900,418 - BBR_RS13905 0.77 +3.2 900,425 + BBR_RS13905 0.78 -0.4 900,425 + BBR_RS13905 0.78 +0.5 900,539 + -0.0 900,540 + -1.2 900,826 + BBR_RS13910 0.38 -0.5 900,932 + BBR_RS13910 0.58 +1.1 900,985 + BBR_RS13910 0.69 -0.3 901,159 + +0.4 901,167 - -0.1 901,171 + +0.1 901,179 - +1.2 901,190 - -1.3 901,205 + +0.7 901,213 - +0.5 901,316 + -0.3 901,326 + +0.2 901,329 - -0.7 901,339 + +0.2 901,339 - -0.7 901,417 + +1.6 901,417 + +0.5 901,417 + -0.3 901,417 + -0.1 901,417 + -1.6 901,441 - BBR_RS13915 0.11 +1.5 901,442 + BBR_RS13915 0.11 -0.6 901,442 + BBR_RS13915 0.11 +0.7 901,442 - BBR_RS13915 0.11 +1.2 901,489 + BBR_RS13915 0.15 -0.5 901,539 + BBR_RS13915 0.19 +1.8 901,539 + BBR_RS13915 0.19 +0.4 901,567 + BBR_RS13915 0.22 +0.5 901,573 - BBR_RS13915 0.22 -0.4 901,606 + BBR_RS13915 0.25 +0.1 901,629 - BBR_RS13915 0.27 +0.2 901,676 - BBR_RS13915 0.30 +1.5 901,693 + BBR_RS13915 0.32 +0.2 901,693 - BBR_RS13915 0.32 +0.4 901,719 - BBR_RS13915 0.34 +0.4 901,728 - BBR_RS13915 0.35 -0.8 901,750 + BBR_RS13915 0.36 +0.7 901,774 + BBR_RS13915 0.38 -0.0 901,799 + BBR_RS13915 0.40 -0.2 901,809 + BBR_RS13915 0.41 -0.4 901,839 + BBR_RS13915 0.44 +1.6 901,851 - BBR_RS13915 0.44 +0.9 901,905 + BBR_RS13915 0.49 -1.2 901,905 + BBR_RS13915 0.49 +0.6 901,911 + BBR_RS13915 0.49 -1.6 901,919 - BBR_RS13915 0.50 +0.6 901,940 - BBR_RS13915 0.52 +0.7 901,998 - BBR_RS13915 0.56 +0.9 902,019 - BBR_RS13915 0.58 -0.2 902,021 + BBR_RS13915 0.58 +0.0 902,094 - BBR_RS13915 0.64 -0.9 902,105 + BBR_RS13915 0.65 -1.1 902,115 + BBR_RS13915 0.66 +0.7 902,122 + BBR_RS13915 0.66 +0.1 902,123 - BBR_RS13915 0.66 +1.3 902,123 - BBR_RS13915 0.66 +0.3 902,123 - BBR_RS13915 0.66 -0.5 902,123 - BBR_RS13915 0.66 +0.3 902,132 + BBR_RS13915 0.67 -0.3 902,137 + BBR_RS13915 0.68 -0.4 902,142 + BBR_RS13915 0.68 +0.6 902,142 + BBR_RS13915 0.68 +0.3
Or see this region's nucleotide sequence