Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13835 and BBR_RS13840 are separated by 123 nucleotides BBR_RS13840 and BBR_RS13845 are separated by 91 nucleotides BBR_RS13845 and BBR_RS13850 are separated by 570 nucleotides
BBR_RS13835: BBR_RS13835 - FAD-dependent oxidoreductase, at 882,198 to 883,691
_RS13835
BBR_RS13840: BBR_RS13840 - DUF3043 domain-containing protein, at 883,815 to 884,435
_RS13840
BBR_RS13845: BBR_RS13845 - dipeptidase, at 884,527 to 885,894
_RS13845
BBR_RS13850: BBR_RS13850 - ABC transporter permease, at 886,465 to 887,064
_RS13850
Position (kb) 884
885
886 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 883.535 kb on - strand, within BBR_RS13835 at 883.557 kb on + strand at 883.565 kb on - strand at 883.565 kb on - strand at 883.565 kb on - strand at 883.566 kb on + strand at 883.607 kb on - strand at 883.622 kb on - strand at 883.722 kb on + strand at 883.723 kb on + strand at 883.738 kb on - strand at 883.797 kb on + strand at 883.843 kb on + strand at 883.872 kb on - strand at 883.926 kb on - strand, within BBR_RS13840 at 883.959 kb on + strand, within BBR_RS13840 at 883.968 kb on - strand, within BBR_RS13840 at 883.968 kb on - strand, within BBR_RS13840 at 884.053 kb on - strand, within BBR_RS13840 at 884.055 kb on - strand, within BBR_RS13840 at 884.190 kb on - strand, within BBR_RS13840 at 884.200 kb on + strand, within BBR_RS13840 at 884.322 kb on - strand, within BBR_RS13840 at 884.423 kb on + strand at 884.431 kb on - strand at 884.475 kb on + strand at 884.475 kb on + strand at 884.475 kb on + strand at 884.509 kb on + strand at 884.550 kb on - strand at 884.758 kb on + strand, within BBR_RS13845 at 884.797 kb on + strand, within BBR_RS13845 at 884.802 kb on + strand, within BBR_RS13845 at 884.814 kb on + strand, within BBR_RS13845 at 884.815 kb on - strand, within BBR_RS13845 at 884.821 kb on + strand, within BBR_RS13845 at 884.898 kb on - strand, within BBR_RS13845 at 884.966 kb on + strand, within BBR_RS13845 at 884.987 kb on - strand, within BBR_RS13845 at 885.004 kb on + strand, within BBR_RS13845 at 885.031 kb on - strand, within BBR_RS13845 at 885.089 kb on - strand, within BBR_RS13845 at 885.109 kb on + strand, within BBR_RS13845 at 885.114 kb on - strand, within BBR_RS13845 at 885.128 kb on - strand, within BBR_RS13845 at 885.136 kb on - strand, within BBR_RS13845 at 885.146 kb on + strand, within BBR_RS13845 at 885.180 kb on + strand, within BBR_RS13845 at 885.193 kb on - strand, within BBR_RS13845 at 885.218 kb on + strand, within BBR_RS13845 at 885.237 kb on + strand, within BBR_RS13845 at 885.420 kb on + strand, within BBR_RS13845 at 885.454 kb on + strand, within BBR_RS13845 at 885.462 kb on - strand, within BBR_RS13845 at 885.472 kb on - strand, within BBR_RS13845 at 885.584 kb on + strand, within BBR_RS13845 at 885.586 kb on + strand, within BBR_RS13845 at 885.598 kb on + strand, within BBR_RS13845 at 885.621 kb on + strand, within BBR_RS13845 at 885.623 kb on + strand, within BBR_RS13845 at 885.629 kb on - strand, within BBR_RS13845 at 885.677 kb on - strand, within BBR_RS13845 at 885.689 kb on + strand, within BBR_RS13845 at 885.697 kb on - strand, within BBR_RS13845 at 885.698 kb on + strand, within BBR_RS13845 at 885.709 kb on + strand, within BBR_RS13845 at 885.719 kb on + strand, within BBR_RS13845 at 885.729 kb on + strand, within BBR_RS13845 at 885.734 kb on + strand, within BBR_RS13845 at 885.742 kb on - strand, within BBR_RS13845 at 885.771 kb on - strand at 885.810 kb on - strand at 885.812 kb on + strand at 885.820 kb on - strand at 885.820 kb on - strand at 885.820 kb on - strand at 885.820 kb on - strand at 885.820 kb on - strand at 885.904 kb on + strand at 885.904 kb on + strand at 885.904 kb on + strand at 885.904 kb on + strand at 885.904 kb on + strand at 885.910 kb on + strand at 885.912 kb on - strand at 885.912 kb on - strand at 885.912 kb on - strand at 885.912 kb on - strand at 885.912 kb on - strand at 885.912 kb on - strand at 885.912 kb on - strand at 885.912 kb on - strand at 885.912 kb on - strand at 885.912 kb on - strand at 885.957 kb on - strand at 886.011 kb on + strand at 886.036 kb on + strand at 886.036 kb on + strand at 886.050 kb on + strand at 886.090 kb on + strand at 886.123 kb on + strand at 886.275 kb on - strand at 886.275 kb on - strand at 886.283 kb on - strand at 886.290 kb on - strand at 886.298 kb on - strand at 886.332 kb on - strand at 886.342 kb on + strand at 886.359 kb on - strand at 886.359 kb on - strand at 886.406 kb on + strand at 886.427 kb on + strand at 886.427 kb on + strand at 886.461 kb on + strand at 886.561 kb on + strand, within BBR_RS13850 at 886.572 kb on + strand, within BBR_RS13850 at 886.573 kb on + strand, within BBR_RS13850 at 886.590 kb on + strand, within BBR_RS13850 at 886.688 kb on + strand, within BBR_RS13850 at 886.691 kb on - strand, within BBR_RS13850 at 886.722 kb on + strand, within BBR_RS13850 at 886.751 kb on - strand, within BBR_RS13850 at 886.798 kb on + strand, within BBR_RS13850 at 886.844 kb on + strand, within BBR_RS13850 at 886.844 kb on + strand, within BBR_RS13850 at 886.844 kb on + strand, within BBR_RS13850 at 886.885 kb on + strand, within BBR_RS13850 at 886.890 kb on + strand, within BBR_RS13850
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 883,535 - BBR_RS13835 0.89 +0.2 883,557 + +0.0 883,565 - -2.5 883,565 - -2.2 883,565 - -0.5 883,566 + -0.3 883,607 - -1.0 883,622 - +0.9 883,722 + +1.7 883,723 + -0.0 883,738 - -0.1 883,797 + +0.5 883,843 + -0.0 883,872 - +0.2 883,926 - BBR_RS13840 0.18 -0.2 883,959 + BBR_RS13840 0.23 -0.8 883,968 - BBR_RS13840 0.25 +0.1 883,968 - BBR_RS13840 0.25 +0.4 884,053 - BBR_RS13840 0.38 -0.0 884,055 - BBR_RS13840 0.39 -0.6 884,190 - BBR_RS13840 0.60 -0.8 884,200 + BBR_RS13840 0.62 -0.4 884,322 - BBR_RS13840 0.82 +0.1 884,423 + -1.5 884,431 - +0.6 884,475 + +0.7 884,475 + -0.6 884,475 + -0.0 884,509 + +0.3 884,550 - -0.3 884,758 + BBR_RS13845 0.17 -1.2 884,797 + BBR_RS13845 0.20 -1.1 884,802 + BBR_RS13845 0.20 -2.8 884,814 + BBR_RS13845 0.21 -0.2 884,815 - BBR_RS13845 0.21 -1.1 884,821 + BBR_RS13845 0.21 -0.2 884,898 - BBR_RS13845 0.27 -0.5 884,966 + BBR_RS13845 0.32 -1.6 884,987 - BBR_RS13845 0.34 -0.3 885,004 + BBR_RS13845 0.35 +0.8 885,031 - BBR_RS13845 0.37 +0.1 885,089 - BBR_RS13845 0.41 -1.2 885,109 + BBR_RS13845 0.43 +1.7 885,114 - BBR_RS13845 0.43 +1.8 885,128 - BBR_RS13845 0.44 +0.0 885,136 - BBR_RS13845 0.45 -0.1 885,146 + BBR_RS13845 0.45 -1.4 885,180 + BBR_RS13845 0.48 -1.1 885,193 - BBR_RS13845 0.49 -0.1 885,218 + BBR_RS13845 0.51 +0.4 885,237 + BBR_RS13845 0.52 -0.5 885,420 + BBR_RS13845 0.65 -0.5 885,454 + BBR_RS13845 0.68 -2.2 885,462 - BBR_RS13845 0.68 +0.6 885,472 - BBR_RS13845 0.69 -0.8 885,584 + BBR_RS13845 0.77 +0.3 885,586 + BBR_RS13845 0.77 -1.6 885,598 + BBR_RS13845 0.78 -0.6 885,621 + BBR_RS13845 0.80 -2.9 885,623 + BBR_RS13845 0.80 -1.8 885,629 - BBR_RS13845 0.81 +1.1 885,677 - BBR_RS13845 0.84 -0.7 885,689 + BBR_RS13845 0.85 -0.9 885,697 - BBR_RS13845 0.86 +0.6 885,698 + BBR_RS13845 0.86 -0.4 885,709 + BBR_RS13845 0.86 -0.3 885,719 + BBR_RS13845 0.87 -0.7 885,729 + BBR_RS13845 0.88 -0.4 885,734 + BBR_RS13845 0.88 -0.8 885,742 - BBR_RS13845 0.89 -0.3 885,771 - +0.1 885,810 - +0.3 885,812 + -0.5 885,820 - -0.4 885,820 - -0.5 885,820 - -0.1 885,820 - -0.3 885,820 - -1.2 885,904 + -1.9 885,904 + +0.2 885,904 + -0.6 885,904 + -0.2 885,904 + +0.6 885,910 + +0.4 885,912 - -0.5 885,912 - +0.0 885,912 - +1.5 885,912 - -0.1 885,912 - -0.2 885,912 - -1.7 885,912 - +0.3 885,912 - -0.6 885,912 - -0.2 885,912 - +0.2 885,957 - +0.6 886,011 + -0.3 886,036 + +0.3 886,036 + -0.1 886,050 + +0.6 886,090 + -0.6 886,123 + -0.0 886,275 - +0.6 886,275 - +0.3 886,283 - -0.1 886,290 - -0.1 886,298 - -0.1 886,332 - +0.0 886,342 + -0.8 886,359 - +0.6 886,359 - +0.6 886,406 + -0.5 886,427 + +0.6 886,427 + -0.4 886,461 + +0.4 886,561 + BBR_RS13850 0.16 -0.5 886,572 + BBR_RS13850 0.18 +0.3 886,573 + BBR_RS13850 0.18 -0.2 886,590 + BBR_RS13850 0.21 -0.9 886,688 + BBR_RS13850 0.37 +0.3 886,691 - BBR_RS13850 0.38 +0.3 886,722 + BBR_RS13850 0.43 -0.8 886,751 - BBR_RS13850 0.48 +0.6 886,798 + BBR_RS13850 0.56 -0.0 886,844 + BBR_RS13850 0.63 -0.3 886,844 + BBR_RS13850 0.63 +1.2 886,844 + BBR_RS13850 0.63 -0.2 886,885 + BBR_RS13850 0.70 +0.4 886,890 + BBR_RS13850 0.71 -1.1
Or see this region's nucleotide sequence