Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13825 and BBR_RS13830 are separated by 303 nucleotides BBR_RS13830 and BBR_RS13835 are separated by 64 nucleotides
BBR_RS13825: BBR_RS13825 - type I glutamate--ammonia ligase, at 879,611 to 881,047
_RS13825
BBR_RS13830: BBR_RS13830 - DUF4191 domain-containing protein, at 881,351 to 882,133
_RS13830
BBR_RS13835: BBR_RS13835 - FAD-dependent oxidoreductase, at 882,198 to 883,691
_RS13835
Position (kb) 881
882
883 Strain fitness (log2 ratio) -2
-1
0
1
2 at 881.078 kb on - strand at 881.220 kb on - strand at 881.220 kb on - strand at 881.245 kb on - strand at 881.277 kb on - strand at 881.284 kb on + strand at 881.401 kb on - strand at 881.435 kb on - strand, within BBR_RS13830 at 882.113 kb on - strand at 882.256 kb on - strand at 882.279 kb on - strand at 882.279 kb on - strand at 882.380 kb on + strand, within BBR_RS13835 at 882.388 kb on + strand, within BBR_RS13835 at 882.388 kb on - strand, within BBR_RS13835 at 882.460 kb on - strand, within BBR_RS13835 at 882.461 kb on - strand, within BBR_RS13835 at 882.549 kb on + strand, within BBR_RS13835 at 882.549 kb on + strand, within BBR_RS13835 at 882.561 kb on + strand, within BBR_RS13835 at 882.575 kb on + strand, within BBR_RS13835 at 882.642 kb on - strand, within BBR_RS13835 at 882.662 kb on + strand, within BBR_RS13835 at 882.667 kb on - strand, within BBR_RS13835 at 882.673 kb on - strand, within BBR_RS13835 at 882.673 kb on - strand, within BBR_RS13835 at 882.766 kb on + strand, within BBR_RS13835 at 882.769 kb on + strand, within BBR_RS13835 at 882.769 kb on + strand, within BBR_RS13835 at 882.774 kb on - strand, within BBR_RS13835 at 882.840 kb on + strand, within BBR_RS13835 at 882.840 kb on + strand, within BBR_RS13835 at 882.848 kb on - strand, within BBR_RS13835 at 882.848 kb on - strand, within BBR_RS13835 at 882.868 kb on + strand, within BBR_RS13835 at 882.899 kb on - strand, within BBR_RS13835 at 882.899 kb on - strand, within BBR_RS13835 at 882.951 kb on + strand, within BBR_RS13835 at 882.985 kb on - strand, within BBR_RS13835 at 883.014 kb on + strand, within BBR_RS13835 at 883.088 kb on - strand, within BBR_RS13835
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 881,078 - -0.7 881,220 - -0.2 881,220 - +0.6 881,245 - -0.2 881,277 - +0.5 881,284 + +0.3 881,401 - -1.0 881,435 - BBR_RS13830 0.11 -0.3 882,113 - -0.4 882,256 - +1.4 882,279 - -0.0 882,279 - -0.3 882,380 + BBR_RS13835 0.12 -1.4 882,388 + BBR_RS13835 0.13 -0.9 882,388 - BBR_RS13835 0.13 -0.1 882,460 - BBR_RS13835 0.18 -0.6 882,461 - BBR_RS13835 0.18 -0.3 882,549 + BBR_RS13835 0.23 -0.1 882,549 + BBR_RS13835 0.23 -0.9 882,561 + BBR_RS13835 0.24 +0.6 882,575 + BBR_RS13835 0.25 +0.9 882,642 - BBR_RS13835 0.30 -0.6 882,662 + BBR_RS13835 0.31 +1.1 882,667 - BBR_RS13835 0.31 +0.2 882,673 - BBR_RS13835 0.32 -2.5 882,673 - BBR_RS13835 0.32 -1.1 882,766 + BBR_RS13835 0.38 -0.6 882,769 + BBR_RS13835 0.38 +0.7 882,769 + BBR_RS13835 0.38 +0.6 882,774 - BBR_RS13835 0.39 +2.1 882,840 + BBR_RS13835 0.43 +1.1 882,840 + BBR_RS13835 0.43 -2.3 882,848 - BBR_RS13835 0.44 +1.2 882,848 - BBR_RS13835 0.44 -0.6 882,868 + BBR_RS13835 0.45 -2.4 882,899 - BBR_RS13835 0.47 +0.7 882,899 - BBR_RS13835 0.47 -0.4 882,951 + BBR_RS13835 0.50 +1.1 882,985 - BBR_RS13835 0.53 -1.1 883,014 + BBR_RS13835 0.55 -2.4 883,088 - BBR_RS13835 0.60 +0.0
Or see this region's nucleotide sequence