Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13730 and BBR_RS13735 are separated by 167 nucleotides BBR_RS13735 and BBR_RS13740 are separated by 91 nucleotides BBR_RS13740 and BBR_RS13745 are separated by 107 nucleotides BBR_RS13745 and BBR_RS13750 are separated by 143 nucleotides
BBR_RS13730: BBR_RS13730 - hypothetical protein, at 853,537 to 855,267
_RS13730
BBR_RS13735: BBR_RS13735 - Lon protease, at 855,435 to 856,358
_RS13735
BBR_RS13740: BBR_RS13740 - DUF3052 domain-containing protein, at 856,450 to 856,866
_RS13740
BBR_RS13745: BBR_RS13745 - tRNA-Val, at 856,974 to 857,047
_RS13745
BBR_RS13750: BBR_RS13750 - DivIVA domain-containing protein, at 857,191 to 858,789
_RS13750
Position (kb) 855
856
857 Strain fitness (log2 ratio) -2
-1
0
1
2 at 854.447 kb on + strand, within BBR_RS13730 at 854.447 kb on + strand, within BBR_RS13730 at 854.470 kb on - strand, within BBR_RS13730 at 854.532 kb on - strand, within BBR_RS13730 at 854.532 kb on - strand, within BBR_RS13730 at 854.532 kb on - strand, within BBR_RS13730 at 854.564 kb on + strand, within BBR_RS13730 at 854.569 kb on + strand, within BBR_RS13730 at 854.572 kb on - strand, within BBR_RS13730 at 854.572 kb on - strand, within BBR_RS13730 at 854.589 kb on + strand, within BBR_RS13730 at 854.615 kb on + strand, within BBR_RS13730 at 854.847 kb on - strand, within BBR_RS13730 at 854.914 kb on + strand, within BBR_RS13730 at 854.956 kb on + strand, within BBR_RS13730 at 854.959 kb on - strand, within BBR_RS13730 at 854.965 kb on + strand, within BBR_RS13730 at 854.984 kb on - strand, within BBR_RS13730 at 854.995 kb on + strand, within BBR_RS13730 at 854.995 kb on + strand, within BBR_RS13730 at 855.042 kb on - strand, within BBR_RS13730 at 855.043 kb on + strand, within BBR_RS13730 at 855.045 kb on + strand, within BBR_RS13730 at 855.051 kb on - strand, within BBR_RS13730 at 855.051 kb on - strand, within BBR_RS13730 at 855.051 kb on - strand, within BBR_RS13730 at 855.068 kb on + strand, within BBR_RS13730 at 855.079 kb on - strand, within BBR_RS13730 at 855.109 kb on - strand at 855.125 kb on + strand at 855.147 kb on - strand at 855.148 kb on - strand at 855.152 kb on - strand at 855.181 kb on - strand at 855.267 kb on - strand at 855.270 kb on + strand at 855.298 kb on - strand at 855.302 kb on + strand at 855.373 kb on + strand at 855.381 kb on - strand at 855.381 kb on - strand at 855.381 kb on - strand at 855.392 kb on - strand at 855.529 kb on + strand, within BBR_RS13735 at 856.326 kb on - strand at 856.462 kb on + strand at 856.506 kb on - strand, within BBR_RS13740 at 856.507 kb on + strand, within BBR_RS13740 at 856.518 kb on + strand, within BBR_RS13740 at 856.566 kb on + strand, within BBR_RS13740 at 856.594 kb on + strand, within BBR_RS13740 at 856.594 kb on + strand, within BBR_RS13740 at 856.659 kb on + strand, within BBR_RS13740 at 856.659 kb on + strand, within BBR_RS13740 at 856.687 kb on - strand, within BBR_RS13740 at 856.774 kb on + strand, within BBR_RS13740 at 856.811 kb on + strand, within BBR_RS13740 at 856.904 kb on + strand at 856.933 kb on + strand at 856.951 kb on - strand at 857.012 kb on - strand, within BBR_RS13745 at 857.017 kb on + strand, within BBR_RS13745 at 857.061 kb on + strand at 857.080 kb on + strand at 857.128 kb on + strand at 857.148 kb on + strand at 857.148 kb on + strand at 857.156 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 854,447 + BBR_RS13730 0.53 -0.5 854,447 + BBR_RS13730 0.53 +0.0 854,470 - BBR_RS13730 0.54 -0.5 854,532 - BBR_RS13730 0.57 +0.2 854,532 - BBR_RS13730 0.57 -0.2 854,532 - BBR_RS13730 0.57 +1.0 854,564 + BBR_RS13730 0.59 -0.7 854,569 + BBR_RS13730 0.60 +0.7 854,572 - BBR_RS13730 0.60 -0.5 854,572 - BBR_RS13730 0.60 +0.7 854,589 + BBR_RS13730 0.61 +1.9 854,615 + BBR_RS13730 0.62 +0.7 854,847 - BBR_RS13730 0.76 +0.5 854,914 + BBR_RS13730 0.80 +0.7 854,956 + BBR_RS13730 0.82 -1.5 854,959 - BBR_RS13730 0.82 -0.2 854,965 + BBR_RS13730 0.82 +1.7 854,984 - BBR_RS13730 0.84 -0.1 854,995 + BBR_RS13730 0.84 -1.0 854,995 + BBR_RS13730 0.84 -1.3 855,042 - BBR_RS13730 0.87 -1.1 855,043 + BBR_RS13730 0.87 -0.6 855,045 + BBR_RS13730 0.87 +1.1 855,051 - BBR_RS13730 0.87 -0.0 855,051 - BBR_RS13730 0.87 -0.8 855,051 - BBR_RS13730 0.87 -0.5 855,068 + BBR_RS13730 0.88 -0.2 855,079 - BBR_RS13730 0.89 +1.3 855,109 - -1.2 855,125 + -1.8 855,147 - +0.3 855,148 - +0.4 855,152 - -1.2 855,181 - -1.0 855,267 - -0.2 855,270 + +0.3 855,298 - +1.0 855,302 + +0.2 855,373 + -1.5 855,381 - +0.4 855,381 - -0.4 855,381 - +0.2 855,392 - -0.1 855,529 + BBR_RS13735 0.10 -0.7 856,326 - -1.7 856,462 + -1.0 856,506 - BBR_RS13740 0.13 +1.8 856,507 + BBR_RS13740 0.14 -1.3 856,518 + BBR_RS13740 0.16 -1.5 856,566 + BBR_RS13740 0.28 -0.8 856,594 + BBR_RS13740 0.35 -0.7 856,594 + BBR_RS13740 0.35 -0.6 856,659 + BBR_RS13740 0.50 +1.4 856,659 + BBR_RS13740 0.50 +0.9 856,687 - BBR_RS13740 0.57 +0.8 856,774 + BBR_RS13740 0.78 -0.1 856,811 + BBR_RS13740 0.87 -1.3 856,904 + +0.7 856,933 + -0.2 856,951 - +0.7 857,012 - BBR_RS13745 0.51 +2.7 857,017 + BBR_RS13745 0.58 +1.1 857,061 + +0.8 857,080 + -0.6 857,128 + -1.8 857,148 + +0.9 857,148 + +1.1 857,156 - +0.4
Or see this region's nucleotide sequence