Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13635 and BBR_RS13640 overlap by 1 nucleotides BBR_RS13640 and BBR_RS13645 are separated by 36 nucleotides BBR_RS13645 and BBR_RS13650 are separated by 33 nucleotides
BBR_RS13635: BBR_RS13635 - carbamoyl-phosphate synthase large subunit, at 831,142 to 834,525
_RS13635
BBR_RS13640: BBR_RS13640 - orotidine-5'-phosphate decarboxylase, at 834,525 to 835,445
_RS13640
BBR_RS13645: BBR_RS13645 - guanylate kinase, at 835,482 to 836,072
_RS13645
BBR_RS13650: BBR_RS13650 - 3'-5' exonuclease, at 836,106 to 836,813
_RS13650
Position (kb) 834
835
836 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 833.552 kb on + strand, within BBR_RS13635 at 833.597 kb on - strand, within BBR_RS13635 at 833.610 kb on + strand, within BBR_RS13635 at 833.649 kb on + strand, within BBR_RS13635 at 833.656 kb on + strand, within BBR_RS13635 at 833.717 kb on + strand, within BBR_RS13635 at 833.717 kb on + strand, within BBR_RS13635 at 833.832 kb on + strand, within BBR_RS13635 at 833.833 kb on + strand, within BBR_RS13635 at 833.892 kb on + strand, within BBR_RS13635 at 833.937 kb on + strand, within BBR_RS13635 at 833.942 kb on + strand, within BBR_RS13635 at 833.974 kb on + strand, within BBR_RS13635 at 833.975 kb on - strand, within BBR_RS13635 at 834.110 kb on + strand, within BBR_RS13635 at 834.125 kb on + strand, within BBR_RS13635 at 834.219 kb on - strand at 834.450 kb on + strand at 834.519 kb on + strand at 834.657 kb on + strand, within BBR_RS13640 at 834.679 kb on + strand, within BBR_RS13640 at 834.679 kb on + strand, within BBR_RS13640 at 834.679 kb on + strand, within BBR_RS13640 at 834.786 kb on + strand, within BBR_RS13640 at 834.800 kb on + strand, within BBR_RS13640 at 834.800 kb on + strand, within BBR_RS13640 at 834.800 kb on + strand, within BBR_RS13640 at 834.813 kb on + strand, within BBR_RS13640 at 834.815 kb on + strand, within BBR_RS13640 at 834.822 kb on + strand, within BBR_RS13640 at 834.834 kb on + strand, within BBR_RS13640 at 834.893 kb on + strand, within BBR_RS13640 at 834.893 kb on + strand, within BBR_RS13640 at 835.021 kb on + strand, within BBR_RS13640 at 835.036 kb on + strand, within BBR_RS13640 at 835.121 kb on + strand, within BBR_RS13640 at 835.141 kb on + strand, within BBR_RS13640 at 835.156 kb on + strand, within BBR_RS13640 at 835.174 kb on + strand, within BBR_RS13640 at 835.200 kb on + strand, within BBR_RS13640 at 835.281 kb on + strand, within BBR_RS13640 at 835.448 kb on - strand at 835.493 kb on + strand at 836.057 kb on + strand at 836.109 kb on + strand at 836.117 kb on - strand at 836.130 kb on + strand at 836.148 kb on + strand at 836.148 kb on + strand at 836.156 kb on - strand at 836.156 kb on - strand at 836.165 kb on + strand at 836.165 kb on + strand at 836.243 kb on - strand, within BBR_RS13650 at 836.270 kb on - strand, within BBR_RS13650 at 836.272 kb on - strand, within BBR_RS13650 at 836.276 kb on + strand, within BBR_RS13650 at 836.324 kb on + strand, within BBR_RS13650 at 836.324 kb on + strand, within BBR_RS13650 at 836.339 kb on - strand, within BBR_RS13650 at 836.409 kb on - strand, within BBR_RS13650
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 833,552 + BBR_RS13635 0.71 -1.1 833,597 - BBR_RS13635 0.73 -0.2 833,610 + BBR_RS13635 0.73 +0.1 833,649 + BBR_RS13635 0.74 -0.3 833,656 + BBR_RS13635 0.74 -1.4 833,717 + BBR_RS13635 0.76 -0.4 833,717 + BBR_RS13635 0.76 -1.3 833,832 + BBR_RS13635 0.79 -2.0 833,833 + BBR_RS13635 0.80 -0.5 833,892 + BBR_RS13635 0.81 -1.0 833,937 + BBR_RS13635 0.83 -1.1 833,942 + BBR_RS13635 0.83 -1.0 833,974 + BBR_RS13635 0.84 +0.1 833,975 - BBR_RS13635 0.84 -0.6 834,110 + BBR_RS13635 0.88 -0.4 834,125 + BBR_RS13635 0.88 -1.6 834,219 - +0.9 834,450 + +0.4 834,519 + -0.7 834,657 + BBR_RS13640 0.14 -3.1 834,679 + BBR_RS13640 0.17 -2.7 834,679 + BBR_RS13640 0.17 -3.2 834,679 + BBR_RS13640 0.17 -2.4 834,786 + BBR_RS13640 0.28 -2.9 834,800 + BBR_RS13640 0.30 -1.4 834,800 + BBR_RS13640 0.30 -1.1 834,800 + BBR_RS13640 0.30 -1.0 834,813 + BBR_RS13640 0.31 -3.1 834,815 + BBR_RS13640 0.31 -1.8 834,822 + BBR_RS13640 0.32 -2.9 834,834 + BBR_RS13640 0.34 -2.7 834,893 + BBR_RS13640 0.40 -0.7 834,893 + BBR_RS13640 0.40 +1.3 835,021 + BBR_RS13640 0.54 -0.6 835,036 + BBR_RS13640 0.55 -1.6 835,121 + BBR_RS13640 0.65 +0.7 835,141 + BBR_RS13640 0.67 -0.8 835,156 + BBR_RS13640 0.69 -0.7 835,174 + BBR_RS13640 0.70 -1.2 835,200 + BBR_RS13640 0.73 -3.2 835,281 + BBR_RS13640 0.82 +0.1 835,448 - +3.7 835,493 + -0.0 836,057 + +0.4 836,109 + +0.2 836,117 - -0.5 836,130 + +1.1 836,148 + +0.7 836,148 + -1.0 836,156 - +0.7 836,156 - -0.7 836,165 + -0.3 836,165 + -0.2 836,243 - BBR_RS13650 0.19 +0.0 836,270 - BBR_RS13650 0.23 +1.2 836,272 - BBR_RS13650 0.23 -0.0 836,276 + BBR_RS13650 0.24 +0.2 836,324 + BBR_RS13650 0.31 -0.9 836,324 + BBR_RS13650 0.31 -1.0 836,339 - BBR_RS13650 0.33 +1.0 836,409 - BBR_RS13650 0.43 +0.0
Or see this region's nucleotide sequence