Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13630 and BBR_RS13635 are separated by 1 nucleotides BBR_RS13635 and BBR_RS13640 overlap by 1 nucleotides BBR_RS13640 and BBR_RS13645 are separated by 36 nucleotides
BBR_RS13630: BBR_RS13630 - carbamoyl-phosphate synthase small subunit, at 829,917 to 831,140
_RS13630
BBR_RS13635: BBR_RS13635 - carbamoyl-phosphate synthase large subunit, at 831,142 to 834,525
_RS13635
BBR_RS13640: BBR_RS13640 - orotidine-5'-phosphate decarboxylase, at 834,525 to 835,445
_RS13640
BBR_RS13645: BBR_RS13645 - guanylate kinase, at 835,482 to 836,072
_RS13645
Position (kb) 831
832
833
834
835 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 830.165 kb on + strand, within BBR_RS13630 at 830.180 kb on + strand, within BBR_RS13630 at 830.194 kb on + strand, within BBR_RS13630 at 830.334 kb on + strand, within BBR_RS13630 at 830.393 kb on + strand, within BBR_RS13630 at 830.393 kb on + strand, within BBR_RS13630 at 830.401 kb on - strand, within BBR_RS13630 at 830.448 kb on + strand, within BBR_RS13630 at 830.449 kb on + strand, within BBR_RS13630 at 830.449 kb on + strand, within BBR_RS13630 at 830.483 kb on + strand, within BBR_RS13630 at 830.491 kb on + strand, within BBR_RS13630 at 830.493 kb on + strand, within BBR_RS13630 at 830.687 kb on - strand, within BBR_RS13630 at 830.751 kb on + strand, within BBR_RS13630 at 830.871 kb on + strand, within BBR_RS13630 at 830.871 kb on + strand, within BBR_RS13630 at 830.879 kb on - strand, within BBR_RS13630 at 830.879 kb on - strand, within BBR_RS13630 at 831.006 kb on + strand, within BBR_RS13630 at 831.014 kb on - strand, within BBR_RS13630 at 831.016 kb on + strand, within BBR_RS13630 at 831.097 kb on - strand at 831.114 kb on + strand at 831.140 kb on - strand at 831.300 kb on + strand at 831.420 kb on + strand at 831.497 kb on + strand, within BBR_RS13635 at 831.532 kb on - strand, within BBR_RS13635 at 831.593 kb on + strand, within BBR_RS13635 at 831.593 kb on + strand, within BBR_RS13635 at 831.601 kb on - strand, within BBR_RS13635 at 831.601 kb on - strand, within BBR_RS13635 at 831.601 kb on - strand, within BBR_RS13635 at 831.601 kb on - strand, within BBR_RS13635 at 831.601 kb on - strand, within BBR_RS13635 at 831.899 kb on + strand, within BBR_RS13635 at 831.922 kb on + strand, within BBR_RS13635 at 832.008 kb on + strand, within BBR_RS13635 at 832.045 kb on - strand, within BBR_RS13635 at 832.164 kb on + strand, within BBR_RS13635 at 832.255 kb on + strand, within BBR_RS13635 at 832.340 kb on + strand, within BBR_RS13635 at 832.348 kb on - strand, within BBR_RS13635 at 832.410 kb on + strand, within BBR_RS13635 at 832.447 kb on + strand, within BBR_RS13635 at 832.452 kb on + strand, within BBR_RS13635 at 832.555 kb on + strand, within BBR_RS13635 at 832.716 kb on + strand, within BBR_RS13635 at 832.734 kb on - strand, within BBR_RS13635 at 832.769 kb on + strand, within BBR_RS13635 at 832.863 kb on - strand, within BBR_RS13635 at 832.863 kb on - strand, within BBR_RS13635 at 832.863 kb on - strand, within BBR_RS13635 at 832.941 kb on + strand, within BBR_RS13635 at 832.941 kb on + strand, within BBR_RS13635 at 832.970 kb on + strand, within BBR_RS13635 at 833.133 kb on + strand, within BBR_RS13635 at 833.150 kb on + strand, within BBR_RS13635 at 833.218 kb on + strand, within BBR_RS13635 at 833.230 kb on + strand, within BBR_RS13635 at 833.230 kb on + strand, within BBR_RS13635 at 833.233 kb on - strand, within BBR_RS13635 at 833.379 kb on + strand, within BBR_RS13635 at 833.488 kb on + strand, within BBR_RS13635 at 833.512 kb on - strand, within BBR_RS13635 at 833.552 kb on + strand, within BBR_RS13635 at 833.597 kb on - strand, within BBR_RS13635 at 833.610 kb on + strand, within BBR_RS13635 at 833.649 kb on + strand, within BBR_RS13635 at 833.656 kb on + strand, within BBR_RS13635 at 833.717 kb on + strand, within BBR_RS13635 at 833.717 kb on + strand, within BBR_RS13635 at 833.832 kb on + strand, within BBR_RS13635 at 833.833 kb on + strand, within BBR_RS13635 at 833.892 kb on + strand, within BBR_RS13635 at 833.937 kb on + strand, within BBR_RS13635 at 833.942 kb on + strand, within BBR_RS13635 at 833.974 kb on + strand, within BBR_RS13635 at 833.975 kb on - strand, within BBR_RS13635 at 834.110 kb on + strand, within BBR_RS13635 at 834.125 kb on + strand, within BBR_RS13635 at 834.219 kb on - strand at 834.450 kb on + strand at 834.519 kb on + strand at 834.657 kb on + strand, within BBR_RS13640 at 834.679 kb on + strand, within BBR_RS13640 at 834.679 kb on + strand, within BBR_RS13640 at 834.679 kb on + strand, within BBR_RS13640 at 834.786 kb on + strand, within BBR_RS13640 at 834.800 kb on + strand, within BBR_RS13640 at 834.800 kb on + strand, within BBR_RS13640 at 834.800 kb on + strand, within BBR_RS13640 at 834.813 kb on + strand, within BBR_RS13640 at 834.815 kb on + strand, within BBR_RS13640 at 834.822 kb on + strand, within BBR_RS13640 at 834.834 kb on + strand, within BBR_RS13640 at 834.893 kb on + strand, within BBR_RS13640 at 834.893 kb on + strand, within BBR_RS13640 at 835.021 kb on + strand, within BBR_RS13640 at 835.036 kb on + strand, within BBR_RS13640 at 835.121 kb on + strand, within BBR_RS13640 at 835.141 kb on + strand, within BBR_RS13640 at 835.156 kb on + strand, within BBR_RS13640 at 835.174 kb on + strand, within BBR_RS13640 at 835.200 kb on + strand, within BBR_RS13640 at 835.281 kb on + strand, within BBR_RS13640 at 835.448 kb on - strand at 835.493 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 830,165 + BBR_RS13630 0.20 +0.0 830,180 + BBR_RS13630 0.21 +0.5 830,194 + BBR_RS13630 0.23 -1.0 830,334 + BBR_RS13630 0.34 +0.7 830,393 + BBR_RS13630 0.39 -2.1 830,393 + BBR_RS13630 0.39 +0.5 830,401 - BBR_RS13630 0.40 -1.0 830,448 + BBR_RS13630 0.43 +0.1 830,449 + BBR_RS13630 0.43 -0.3 830,449 + BBR_RS13630 0.43 -2.2 830,483 + BBR_RS13630 0.46 -0.7 830,491 + BBR_RS13630 0.47 -2.5 830,493 + BBR_RS13630 0.47 -2.9 830,687 - BBR_RS13630 0.63 +0.9 830,751 + BBR_RS13630 0.68 -2.1 830,871 + BBR_RS13630 0.78 +0.1 830,871 + BBR_RS13630 0.78 -0.3 830,879 - BBR_RS13630 0.79 -0.5 830,879 - BBR_RS13630 0.79 -0.9 831,006 + BBR_RS13630 0.89 +1.1 831,014 - BBR_RS13630 0.90 -0.7 831,016 + BBR_RS13630 0.90 -1.2 831,097 - +0.1 831,114 + -0.2 831,140 - +1.9 831,300 + -1.6 831,420 + -0.1 831,497 + BBR_RS13635 0.10 -1.8 831,532 - BBR_RS13635 0.12 -0.7 831,593 + BBR_RS13635 0.13 -0.1 831,593 + BBR_RS13635 0.13 -0.3 831,601 - BBR_RS13635 0.14 -0.3 831,601 - BBR_RS13635 0.14 -1.9 831,601 - BBR_RS13635 0.14 +0.3 831,601 - BBR_RS13635 0.14 +0.4 831,601 - BBR_RS13635 0.14 -0.4 831,899 + BBR_RS13635 0.22 +0.4 831,922 + BBR_RS13635 0.23 -0.9 832,008 + BBR_RS13635 0.26 +1.5 832,045 - BBR_RS13635 0.27 -1.0 832,164 + BBR_RS13635 0.30 -0.0 832,255 + BBR_RS13635 0.33 -1.8 832,340 + BBR_RS13635 0.35 +0.5 832,348 - BBR_RS13635 0.36 +0.1 832,410 + BBR_RS13635 0.37 -0.7 832,447 + BBR_RS13635 0.39 -2.6 832,452 + BBR_RS13635 0.39 -0.6 832,555 + BBR_RS13635 0.42 +1.5 832,716 + BBR_RS13635 0.47 -2.0 832,734 - BBR_RS13635 0.47 -1.1 832,769 + BBR_RS13635 0.48 -1.4 832,863 - BBR_RS13635 0.51 +1.3 832,863 - BBR_RS13635 0.51 -2.1 832,863 - BBR_RS13635 0.51 +0.5 832,941 + BBR_RS13635 0.53 -1.6 832,941 + BBR_RS13635 0.53 -0.9 832,970 + BBR_RS13635 0.54 +0.1 833,133 + BBR_RS13635 0.59 -1.0 833,150 + BBR_RS13635 0.59 -1.6 833,218 + BBR_RS13635 0.61 -0.3 833,230 + BBR_RS13635 0.62 -1.3 833,230 + BBR_RS13635 0.62 -2.1 833,233 - BBR_RS13635 0.62 +1.0 833,379 + BBR_RS13635 0.66 +0.8 833,488 + BBR_RS13635 0.69 -2.4 833,512 - BBR_RS13635 0.70 -0.9 833,552 + BBR_RS13635 0.71 -1.1 833,597 - BBR_RS13635 0.73 -0.2 833,610 + BBR_RS13635 0.73 +0.1 833,649 + BBR_RS13635 0.74 -0.3 833,656 + BBR_RS13635 0.74 -1.4 833,717 + BBR_RS13635 0.76 -0.4 833,717 + BBR_RS13635 0.76 -1.3 833,832 + BBR_RS13635 0.79 -2.0 833,833 + BBR_RS13635 0.80 -0.5 833,892 + BBR_RS13635 0.81 -1.0 833,937 + BBR_RS13635 0.83 -1.1 833,942 + BBR_RS13635 0.83 -1.0 833,974 + BBR_RS13635 0.84 +0.1 833,975 - BBR_RS13635 0.84 -0.6 834,110 + BBR_RS13635 0.88 -0.4 834,125 + BBR_RS13635 0.88 -1.6 834,219 - +0.9 834,450 + +0.4 834,519 + -0.7 834,657 + BBR_RS13640 0.14 -3.1 834,679 + BBR_RS13640 0.17 -2.7 834,679 + BBR_RS13640 0.17 -3.2 834,679 + BBR_RS13640 0.17 -2.4 834,786 + BBR_RS13640 0.28 -2.9 834,800 + BBR_RS13640 0.30 -1.4 834,800 + BBR_RS13640 0.30 -1.1 834,800 + BBR_RS13640 0.30 -1.0 834,813 + BBR_RS13640 0.31 -3.1 834,815 + BBR_RS13640 0.31 -1.8 834,822 + BBR_RS13640 0.32 -2.9 834,834 + BBR_RS13640 0.34 -2.7 834,893 + BBR_RS13640 0.40 -0.7 834,893 + BBR_RS13640 0.40 +1.3 835,021 + BBR_RS13640 0.54 -0.6 835,036 + BBR_RS13640 0.55 -1.6 835,121 + BBR_RS13640 0.65 +0.7 835,141 + BBR_RS13640 0.67 -0.8 835,156 + BBR_RS13640 0.69 -0.7 835,174 + BBR_RS13640 0.70 -1.2 835,200 + BBR_RS13640 0.73 -3.2 835,281 + BBR_RS13640 0.82 +0.1 835,448 - +3.7 835,493 + -0.0
Or see this region's nucleotide sequence