Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13620 and BBR_RS13625 are separated by 54 nucleotides BBR_RS13625 and BBR_RS13630 are separated by 187 nucleotides BBR_RS13630 and BBR_RS13635 are separated by 1 nucleotides
BBR_RS13620: BBR_RS13620 - elongation factor P, at 828,581 to 829,144
_RS13620
BBR_RS13625: BBR_RS13625 - transcription antitermination factor NusB, at 829,199 to 829,729
_RS13625
BBR_RS13630: BBR_RS13630 - carbamoyl-phosphate synthase small subunit, at 829,917 to 831,140
_RS13630
BBR_RS13635: BBR_RS13635 - carbamoyl-phosphate synthase large subunit, at 831,142 to 834,525
_RS13635
Position (kb) 829
830
831
832 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 829.156 kb on + strand at 829.189 kb on + strand at 829.582 kb on + strand, within BBR_RS13625 at 829.582 kb on + strand, within BBR_RS13625 at 829.646 kb on + strand, within BBR_RS13625 at 829.647 kb on + strand, within BBR_RS13625 at 829.651 kb on + strand, within BBR_RS13625 at 829.714 kb on + strand at 829.868 kb on - strand at 829.931 kb on + strand at 829.974 kb on + strand at 830.002 kb on + strand at 830.165 kb on + strand, within BBR_RS13630 at 830.180 kb on + strand, within BBR_RS13630 at 830.194 kb on + strand, within BBR_RS13630 at 830.334 kb on + strand, within BBR_RS13630 at 830.393 kb on + strand, within BBR_RS13630 at 830.393 kb on + strand, within BBR_RS13630 at 830.401 kb on - strand, within BBR_RS13630 at 830.448 kb on + strand, within BBR_RS13630 at 830.449 kb on + strand, within BBR_RS13630 at 830.449 kb on + strand, within BBR_RS13630 at 830.483 kb on + strand, within BBR_RS13630 at 830.491 kb on + strand, within BBR_RS13630 at 830.493 kb on + strand, within BBR_RS13630 at 830.687 kb on - strand, within BBR_RS13630 at 830.751 kb on + strand, within BBR_RS13630 at 830.871 kb on + strand, within BBR_RS13630 at 830.871 kb on + strand, within BBR_RS13630 at 830.879 kb on - strand, within BBR_RS13630 at 830.879 kb on - strand, within BBR_RS13630 at 831.006 kb on + strand, within BBR_RS13630 at 831.014 kb on - strand, within BBR_RS13630 at 831.016 kb on + strand, within BBR_RS13630 at 831.097 kb on - strand at 831.114 kb on + strand at 831.140 kb on - strand at 831.300 kb on + strand at 831.420 kb on + strand at 831.497 kb on + strand, within BBR_RS13635 at 831.532 kb on - strand, within BBR_RS13635 at 831.593 kb on + strand, within BBR_RS13635 at 831.593 kb on + strand, within BBR_RS13635 at 831.601 kb on - strand, within BBR_RS13635 at 831.601 kb on - strand, within BBR_RS13635 at 831.601 kb on - strand, within BBR_RS13635 at 831.601 kb on - strand, within BBR_RS13635 at 831.601 kb on - strand, within BBR_RS13635 at 831.899 kb on + strand, within BBR_RS13635 at 831.922 kb on + strand, within BBR_RS13635 at 832.008 kb on + strand, within BBR_RS13635 at 832.045 kb on - strand, within BBR_RS13635
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 829,156 + -0.2 829,189 + -0.8 829,582 + BBR_RS13625 0.72 -0.2 829,582 + BBR_RS13625 0.72 +1.6 829,646 + BBR_RS13625 0.84 -0.5 829,647 + BBR_RS13625 0.84 +0.3 829,651 + BBR_RS13625 0.85 -0.4 829,714 + +0.1 829,868 - +1.2 829,931 + +0.9 829,974 + +0.1 830,002 + -0.1 830,165 + BBR_RS13630 0.20 +0.0 830,180 + BBR_RS13630 0.21 +0.5 830,194 + BBR_RS13630 0.23 -1.0 830,334 + BBR_RS13630 0.34 +0.7 830,393 + BBR_RS13630 0.39 -2.1 830,393 + BBR_RS13630 0.39 +0.5 830,401 - BBR_RS13630 0.40 -1.0 830,448 + BBR_RS13630 0.43 +0.1 830,449 + BBR_RS13630 0.43 -0.3 830,449 + BBR_RS13630 0.43 -2.2 830,483 + BBR_RS13630 0.46 -0.7 830,491 + BBR_RS13630 0.47 -2.5 830,493 + BBR_RS13630 0.47 -2.9 830,687 - BBR_RS13630 0.63 +0.9 830,751 + BBR_RS13630 0.68 -2.1 830,871 + BBR_RS13630 0.78 +0.1 830,871 + BBR_RS13630 0.78 -0.3 830,879 - BBR_RS13630 0.79 -0.5 830,879 - BBR_RS13630 0.79 -0.9 831,006 + BBR_RS13630 0.89 +1.1 831,014 - BBR_RS13630 0.90 -0.7 831,016 + BBR_RS13630 0.90 -1.2 831,097 - +0.1 831,114 + -0.2 831,140 - +1.9 831,300 + -1.6 831,420 + -0.1 831,497 + BBR_RS13635 0.10 -1.8 831,532 - BBR_RS13635 0.12 -0.7 831,593 + BBR_RS13635 0.13 -0.1 831,593 + BBR_RS13635 0.13 -0.3 831,601 - BBR_RS13635 0.14 -0.3 831,601 - BBR_RS13635 0.14 -1.9 831,601 - BBR_RS13635 0.14 +0.3 831,601 - BBR_RS13635 0.14 +0.4 831,601 - BBR_RS13635 0.14 -0.4 831,899 + BBR_RS13635 0.22 +0.4 831,922 + BBR_RS13635 0.23 -0.9 832,008 + BBR_RS13635 0.26 +1.5 832,045 - BBR_RS13635 0.27 -1.0
Or see this region's nucleotide sequence