Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13450 and BBR_RS13455 are separated by 81 nucleotides BBR_RS13455 and BBR_RS13460 are separated by 499 nucleotides
BBR_RS13450: BBR_RS13450 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 790,705 to 791,457
_RS13450
BBR_RS13455: BBR_RS13455 - formate-dependent phosphoribosylglycinamide formyltransferase, at 791,539 to 792,840
_RS13455
BBR_RS13460: BBR_RS13460 - aminotransferase, at 793,340 to 794,590
_RS13460
Position (kb) 791
792
793 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 790.616 kb on + strand at 790.662 kb on - strand at 790.673 kb on + strand at 790.688 kb on + strand at 790.724 kb on + strand at 790.730 kb on + strand at 790.786 kb on + strand, within BBR_RS13450 at 791.041 kb on + strand, within BBR_RS13450 at 791.041 kb on + strand, within BBR_RS13450 at 791.098 kb on + strand, within BBR_RS13450 at 791.341 kb on + strand, within BBR_RS13450 at 791.408 kb on + strand at 791.472 kb on - strand at 791.490 kb on + strand at 791.520 kb on - strand at 791.523 kb on + strand at 791.526 kb on - strand at 791.598 kb on - strand at 791.630 kb on + strand at 791.754 kb on - strand, within BBR_RS13455 at 791.791 kb on + strand, within BBR_RS13455 at 791.854 kb on + strand, within BBR_RS13455 at 791.916 kb on - strand, within BBR_RS13455 at 791.940 kb on + strand, within BBR_RS13455 at 792.175 kb on + strand, within BBR_RS13455 at 792.241 kb on - strand, within BBR_RS13455 at 792.304 kb on + strand, within BBR_RS13455 at 792.495 kb on + strand, within BBR_RS13455 at 792.534 kb on - strand, within BBR_RS13455 at 792.549 kb on + strand, within BBR_RS13455 at 792.562 kb on - strand, within BBR_RS13455 at 792.750 kb on - strand at 792.759 kb on + strand at 792.828 kb on - strand at 792.837 kb on - strand at 792.853 kb on - strand at 792.865 kb on + strand at 792.865 kb on + strand at 792.865 kb on + strand at 792.865 kb on + strand at 792.865 kb on + strand at 792.865 kb on + strand at 792.873 kb on - strand at 792.873 kb on - strand at 792.887 kb on + strand at 792.929 kb on - strand at 792.929 kb on - strand at 792.931 kb on - strand at 792.999 kb on + strand at 792.999 kb on + strand at 792.999 kb on + strand at 792.999 kb on + strand at 792.999 kb on + strand at 792.999 kb on + strand at 793.006 kb on - strand at 793.007 kb on - strand at 793.007 kb on - strand at 793.007 kb on - strand at 793.022 kb on - strand at 793.048 kb on + strand at 793.050 kb on + strand at 793.050 kb on + strand at 793.096 kb on + strand at 793.106 kb on + strand at 793.114 kb on - strand at 793.114 kb on - strand at 793.116 kb on + strand at 793.121 kb on + strand at 793.124 kb on - strand at 793.129 kb on + strand at 793.129 kb on + strand at 793.131 kb on + strand at 793.158 kb on + strand at 793.262 kb on + strand at 793.262 kb on + strand at 793.270 kb on - strand at 793.274 kb on + strand at 793.312 kb on + strand at 793.325 kb on - strand at 793.325 kb on - strand at 793.325 kb on - strand at 793.325 kb on - strand at 793.352 kb on + strand at 793.369 kb on + strand at 793.418 kb on - strand at 793.488 kb on + strand, within BBR_RS13460 at 793.597 kb on - strand, within BBR_RS13460 at 793.598 kb on + strand, within BBR_RS13460 at 793.599 kb on - strand, within BBR_RS13460 at 793.614 kb on + strand, within BBR_RS13460 at 793.616 kb on - strand, within BBR_RS13460 at 793.761 kb on - strand, within BBR_RS13460 at 793.821 kb on + strand, within BBR_RS13460 at 793.829 kb on - strand, within BBR_RS13460 at 793.835 kb on + strand, within BBR_RS13460
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 790,616 + -1.1 790,662 - +0.1 790,673 + -0.0 790,688 + -1.2 790,724 + -0.4 790,730 + +0.4 790,786 + BBR_RS13450 0.11 +1.2 791,041 + BBR_RS13450 0.45 -1.3 791,041 + BBR_RS13450 0.45 +0.1 791,098 + BBR_RS13450 0.52 -0.9 791,341 + BBR_RS13450 0.84 -0.1 791,408 + +0.7 791,472 - -0.9 791,490 + +0.4 791,520 - -0.6 791,523 + +0.2 791,526 - +0.3 791,598 - -1.1 791,630 + -1.7 791,754 - BBR_RS13455 0.17 +0.9 791,791 + BBR_RS13455 0.19 +1.2 791,854 + BBR_RS13455 0.24 +0.2 791,916 - BBR_RS13455 0.29 -0.3 791,940 + BBR_RS13455 0.31 +0.9 792,175 + BBR_RS13455 0.49 +0.3 792,241 - BBR_RS13455 0.54 -1.6 792,304 + BBR_RS13455 0.59 -0.0 792,495 + BBR_RS13455 0.73 +1.2 792,534 - BBR_RS13455 0.76 +0.2 792,549 + BBR_RS13455 0.78 +0.2 792,562 - BBR_RS13455 0.79 +0.6 792,750 - -0.3 792,759 + +1.7 792,828 - +0.2 792,837 - -1.7 792,853 - +0.4 792,865 + -0.1 792,865 + -0.1 792,865 + +0.1 792,865 + +1.2 792,865 + +0.1 792,865 + +0.1 792,873 - -0.2 792,873 - -0.6 792,887 + +0.7 792,929 - -0.2 792,929 - -0.8 792,931 - +0.2 792,999 + +0.7 792,999 + +0.5 792,999 + +0.6 792,999 + -2.8 792,999 + -0.5 792,999 + +1.4 793,006 - +2.1 793,007 - +0.6 793,007 - -0.1 793,007 - +0.0 793,022 - -2.8 793,048 + -0.2 793,050 + +0.7 793,050 + -1.6 793,096 + -0.2 793,106 + +0.0 793,114 - -0.2 793,114 - -1.4 793,116 + +0.0 793,121 + -0.3 793,124 - -0.6 793,129 + +1.9 793,129 + -0.5 793,131 + -1.5 793,158 + +0.4 793,262 + -0.4 793,262 + -0.3 793,270 - -0.3 793,274 + +1.6 793,312 + +0.5 793,325 - +1.4 793,325 - +0.2 793,325 - +0.3 793,325 - +1.3 793,352 + -0.5 793,369 + -0.9 793,418 - +0.5 793,488 + BBR_RS13460 0.12 +1.0 793,597 - BBR_RS13460 0.21 -1.0 793,598 + BBR_RS13460 0.21 -0.4 793,599 - BBR_RS13460 0.21 +2.2 793,614 + BBR_RS13460 0.22 -0.7 793,616 - BBR_RS13460 0.22 -0.1 793,761 - BBR_RS13460 0.34 +0.8 793,821 + BBR_RS13460 0.38 -0.3 793,829 - BBR_RS13460 0.39 +0.0 793,835 + BBR_RS13460 0.40 +1.1
Or see this region's nucleotide sequence