Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13445 and BBR_RS13450 are separated by 207 nucleotides BBR_RS13450 and BBR_RS13455 are separated by 81 nucleotides
BBR_RS13445: BBR_RS13445 - phosphoribosylformylglycinamidine synthase, at 786,763 to 790,497
_RS13445
BBR_RS13450: BBR_RS13450 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 790,705 to 791,457
_RS13450
BBR_RS13455: BBR_RS13455 - formate-dependent phosphoribosylglycinamide formyltransferase, at 791,539 to 792,840
_RS13455
Position (kb) 790
791
792 Strain fitness (log2 ratio) -2
-1
0
1 at 789.707 kb on - strand, within BBR_RS13445 at 789.744 kb on + strand, within BBR_RS13445 at 789.799 kb on + strand, within BBR_RS13445 at 789.799 kb on + strand, within BBR_RS13445 at 789.807 kb on - strand, within BBR_RS13445 at 789.807 kb on - strand, within BBR_RS13445 at 789.807 kb on - strand, within BBR_RS13445 at 789.839 kb on + strand, within BBR_RS13445 at 789.842 kb on - strand, within BBR_RS13445 at 789.842 kb on - strand, within BBR_RS13445 at 789.842 kb on - strand, within BBR_RS13445 at 789.847 kb on - strand, within BBR_RS13445 at 789.940 kb on - strand, within BBR_RS13445 at 789.995 kb on - strand, within BBR_RS13445 at 790.004 kb on + strand, within BBR_RS13445 at 790.011 kb on - strand, within BBR_RS13445 at 790.060 kb on - strand, within BBR_RS13445 at 790.072 kb on + strand, within BBR_RS13445 at 790.073 kb on + strand, within BBR_RS13445 at 790.149 kb on + strand at 790.258 kb on + strand at 790.323 kb on + strand at 790.350 kb on + strand at 790.355 kb on - strand at 790.360 kb on + strand at 790.361 kb on + strand at 790.410 kb on + strand at 790.616 kb on + strand at 790.662 kb on - strand at 790.673 kb on + strand at 790.688 kb on + strand at 790.724 kb on + strand at 790.730 kb on + strand at 790.786 kb on + strand, within BBR_RS13450 at 791.041 kb on + strand, within BBR_RS13450 at 791.041 kb on + strand, within BBR_RS13450 at 791.098 kb on + strand, within BBR_RS13450 at 791.341 kb on + strand, within BBR_RS13450 at 791.408 kb on + strand at 791.472 kb on - strand at 791.490 kb on + strand at 791.520 kb on - strand at 791.523 kb on + strand at 791.526 kb on - strand at 791.598 kb on - strand at 791.630 kb on + strand at 791.754 kb on - strand, within BBR_RS13455 at 791.791 kb on + strand, within BBR_RS13455 at 791.854 kb on + strand, within BBR_RS13455 at 791.916 kb on - strand, within BBR_RS13455 at 791.940 kb on + strand, within BBR_RS13455 at 792.175 kb on + strand, within BBR_RS13455 at 792.241 kb on - strand, within BBR_RS13455 at 792.304 kb on + strand, within BBR_RS13455
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 789,707 - BBR_RS13445 0.79 +0.5 789,744 + BBR_RS13445 0.80 -0.4 789,799 + BBR_RS13445 0.81 -0.6 789,799 + BBR_RS13445 0.81 -1.3 789,807 - BBR_RS13445 0.81 +1.1 789,807 - BBR_RS13445 0.81 +0.4 789,807 - BBR_RS13445 0.81 +0.0 789,839 + BBR_RS13445 0.82 +1.2 789,842 - BBR_RS13445 0.82 +0.4 789,842 - BBR_RS13445 0.82 -0.2 789,842 - BBR_RS13445 0.82 -0.1 789,847 - BBR_RS13445 0.83 +0.4 789,940 - BBR_RS13445 0.85 -0.8 789,995 - BBR_RS13445 0.87 +0.9 790,004 + BBR_RS13445 0.87 +0.1 790,011 - BBR_RS13445 0.87 -0.3 790,060 - BBR_RS13445 0.88 -0.2 790,072 + BBR_RS13445 0.89 -0.1 790,073 + BBR_RS13445 0.89 -2.0 790,149 + -2.2 790,258 + -0.9 790,323 + +0.7 790,350 + +0.6 790,355 - -1.8 790,360 + +1.6 790,361 + +0.8 790,410 + +0.3 790,616 + -1.1 790,662 - +0.1 790,673 + -0.0 790,688 + -1.2 790,724 + -0.4 790,730 + +0.4 790,786 + BBR_RS13450 0.11 +1.2 791,041 + BBR_RS13450 0.45 -1.3 791,041 + BBR_RS13450 0.45 +0.1 791,098 + BBR_RS13450 0.52 -0.9 791,341 + BBR_RS13450 0.84 -0.1 791,408 + +0.7 791,472 - -0.9 791,490 + +0.4 791,520 - -0.6 791,523 + +0.2 791,526 - +0.3 791,598 - -1.1 791,630 + -1.7 791,754 - BBR_RS13455 0.17 +0.9 791,791 + BBR_RS13455 0.19 +1.2 791,854 + BBR_RS13455 0.24 +0.2 791,916 - BBR_RS13455 0.29 -0.3 791,940 + BBR_RS13455 0.31 +0.9 792,175 + BBR_RS13455 0.49 +0.3 792,241 - BBR_RS13455 0.54 -1.6 792,304 + BBR_RS13455 0.59 -0.0
Or see this region's nucleotide sequence