Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS13435

Experiment: Recovery broth with Tiamulin 2e-04 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS13425 and BBR_RS13430 are separated by 83 nucleotidesBBR_RS13430 and BBR_RS13435 overlap by 4 nucleotidesBBR_RS13435 and BBR_RS13440 overlap by 28 nucleotides BBR_RS13425: BBR_RS13425 - hypothetical protein, at 782,393 to 783,205 _RS13425 BBR_RS13430: BBR_RS13430 - hypothetical protein, at 783,289 to 783,948 _RS13430 BBR_RS13435: BBR_RS13435 - hypothetical protein, at 783,945 to 785,315 _RS13435 BBR_RS13440: BBR_RS13440 - alpha/beta hydrolase, at 785,288 to 786,625 _RS13440 Position (kb) 783 784 785 786Strain fitness (log2 ratio) -2 -1 0 1at 782.978 kb on + strand, within BBR_RS13425at 782.989 kb on - strand, within BBR_RS13425at 783.079 kb on - strand, within BBR_RS13425at 783.089 kb on + strand, within BBR_RS13425at 783.094 kb on + strand, within BBR_RS13425at 783.106 kb on + strand, within BBR_RS13425at 783.166 kb on + strandat 783.174 kb on - strandat 783.180 kb on - strandat 783.180 kb on - strandat 783.187 kb on + strandat 783.199 kb on + strandat 783.199 kb on + strandat 783.258 kb on - strandat 783.385 kb on - strand, within BBR_RS13430at 783.391 kb on - strand, within BBR_RS13430at 783.428 kb on - strand, within BBR_RS13430at 783.434 kb on + strand, within BBR_RS13430at 783.444 kb on - strand, within BBR_RS13430at 783.488 kb on + strand, within BBR_RS13430at 783.488 kb on + strand, within BBR_RS13430at 783.497 kb on - strand, within BBR_RS13430at 783.513 kb on + strand, within BBR_RS13430at 783.530 kb on + strand, within BBR_RS13430at 783.533 kb on - strand, within BBR_RS13430at 783.544 kb on + strand, within BBR_RS13430at 783.544 kb on + strand, within BBR_RS13430at 783.580 kb on - strand, within BBR_RS13430at 783.593 kb on + strand, within BBR_RS13430at 783.595 kb on + strand, within BBR_RS13430at 783.598 kb on + strand, within BBR_RS13430at 783.601 kb on - strand, within BBR_RS13430at 783.601 kb on - strand, within BBR_RS13430at 783.605 kb on + strand, within BBR_RS13430at 783.700 kb on - strand, within BBR_RS13430at 783.700 kb on - strand, within BBR_RS13430at 783.751 kb on + strand, within BBR_RS13430at 783.840 kb on - strand, within BBR_RS13430at 783.857 kb on + strand, within BBR_RS13430at 783.900 kb on + strandat 783.900 kb on + strandat 783.942 kb on + strandat 783.942 kb on + strandat 783.960 kb on + strandat 784.007 kb on + strandat 784.009 kb on - strandat 784.090 kb on + strand, within BBR_RS13435at 784.174 kb on - strand, within BBR_RS13435at 784.187 kb on + strand, within BBR_RS13435at 784.195 kb on - strand, within BBR_RS13435at 784.243 kb on + strand, within BBR_RS13435at 784.243 kb on + strand, within BBR_RS13435at 784.248 kb on + strand, within BBR_RS13435at 784.253 kb on + strand, within BBR_RS13435at 784.256 kb on - strand, within BBR_RS13435at 784.256 kb on - strand, within BBR_RS13435at 784.256 kb on - strand, within BBR_RS13435at 784.263 kb on - strand, within BBR_RS13435at 784.310 kb on - strand, within BBR_RS13435at 784.355 kb on + strand, within BBR_RS13435at 784.364 kb on - strand, within BBR_RS13435at 784.378 kb on - strand, within BBR_RS13435at 784.422 kb on - strand, within BBR_RS13435at 784.427 kb on - strand, within BBR_RS13435at 784.431 kb on + strand, within BBR_RS13435at 784.494 kb on + strand, within BBR_RS13435at 784.502 kb on - strand, within BBR_RS13435at 784.502 kb on - strand, within BBR_RS13435at 784.502 kb on - strand, within BBR_RS13435at 784.632 kb on + strand, within BBR_RS13435at 784.658 kb on + strand, within BBR_RS13435at 784.658 kb on + strand, within BBR_RS13435at 784.664 kb on - strand, within BBR_RS13435at 784.673 kb on + strand, within BBR_RS13435at 784.698 kb on + strand, within BBR_RS13435at 784.698 kb on + strand, within BBR_RS13435at 784.706 kb on - strand, within BBR_RS13435at 784.708 kb on + strand, within BBR_RS13435at 784.845 kb on + strand, within BBR_RS13435at 784.870 kb on - strand, within BBR_RS13435at 784.872 kb on + strand, within BBR_RS13435at 784.906 kb on - strand, within BBR_RS13435at 784.915 kb on + strand, within BBR_RS13435at 784.915 kb on + strand, within BBR_RS13435at 784.923 kb on + strand, within BBR_RS13435at 784.929 kb on - strand, within BBR_RS13435at 784.933 kb on + strand, within BBR_RS13435at 784.941 kb on - strand, within BBR_RS13435at 784.941 kb on - strand, within BBR_RS13435at 784.941 kb on - strand, within BBR_RS13435at 784.943 kb on + strand, within BBR_RS13435at 785.110 kb on - strand, within BBR_RS13435at 785.110 kb on - strand, within BBR_RS13435at 785.116 kb on - strand, within BBR_RS13435at 785.193 kb on + strandat 785.201 kb on - strandat 785.257 kb on + strandat 785.266 kb on + strandat 785.266 kb on + strandat 785.266 kb on + strandat 785.274 kb on - strandat 785.279 kb on - strandat 785.300 kb on + strandat 785.300 kb on + strandat 785.300 kb on + strandat 785.334 kb on + strandat 785.334 kb on + strandat 785.417 kb on + strandat 785.417 kb on + strandat 785.481 kb on - strand, within BBR_RS13440at 785.493 kb on - strand, within BBR_RS13440at 785.539 kb on - strand, within BBR_RS13440at 785.546 kb on + strand, within BBR_RS13440at 785.546 kb on + strand, within BBR_RS13440at 785.554 kb on - strand, within BBR_RS13440at 785.556 kb on + strand, within BBR_RS13440at 785.560 kb on + strand, within BBR_RS13440at 785.564 kb on - strand, within BBR_RS13440at 785.568 kb on - strand, within BBR_RS13440at 785.568 kb on - strand, within BBR_RS13440at 785.568 kb on - strand, within BBR_RS13440at 785.583 kb on - strand, within BBR_RS13440at 785.587 kb on + strand, within BBR_RS13440at 785.587 kb on + strand, within BBR_RS13440at 785.587 kb on + strand, within BBR_RS13440at 785.587 kb on + strand, within BBR_RS13440at 785.595 kb on - strand, within BBR_RS13440at 785.595 kb on - strand, within BBR_RS13440at 785.650 kb on + strand, within BBR_RS13440at 785.653 kb on - strand, within BBR_RS13440at 785.667 kb on - strand, within BBR_RS13440at 785.669 kb on + strand, within BBR_RS13440at 785.672 kb on - strand, within BBR_RS13440at 785.672 kb on - strand, within BBR_RS13440at 785.672 kb on - strand, within BBR_RS13440at 785.677 kb on - strand, within BBR_RS13440at 785.677 kb on - strand, within BBR_RS13440at 785.712 kb on + strand, within BBR_RS13440at 785.712 kb on + strand, within BBR_RS13440at 785.726 kb on + strand, within BBR_RS13440at 785.733 kb on + strand, within BBR_RS13440at 785.734 kb on + strand, within BBR_RS13440at 785.734 kb on + strand, within BBR_RS13440at 785.747 kb on + strand, within BBR_RS13440at 785.769 kb on + strand, within BBR_RS13440at 785.883 kb on + strand, within BBR_RS13440at 785.934 kb on - strand, within BBR_RS13440at 786.057 kb on - strand, within BBR_RS13440at 786.064 kb on + strand, within BBR_RS13440at 786.069 kb on + strand, within BBR_RS13440at 786.101 kb on + strand, within BBR_RS13440at 786.109 kb on - strand, within BBR_RS13440at 786.177 kb on + strand, within BBR_RS13440at 786.208 kb on + strand, within BBR_RS13440at 786.216 kb on - strand, within BBR_RS13440at 786.216 kb on - strand, within BBR_RS13440at 786.221 kb on - strand, within BBR_RS13440at 786.221 kb on - strand, within BBR_RS13440at 786.230 kb on - strand, within BBR_RS13440at 786.308 kb on + strand, within BBR_RS13440at 786.314 kb on + strand, within BBR_RS13440

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Per-strain Table

Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM
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782,978 + BBR_RS13425 0.72 +0.6
782,989 - BBR_RS13425 0.73 +1.4
783,079 - BBR_RS13425 0.84 +0.1
783,089 + BBR_RS13425 0.86 -0.4
783,094 + BBR_RS13425 0.86 -0.6
783,106 + BBR_RS13425 0.88 +0.2
783,166 + +0.6
783,174 - -0.0
783,180 - +0.7
783,180 - +1.2
783,187 + +0.4
783,199 + -0.8
783,199 + +0.2
783,258 - -1.2
783,385 - BBR_RS13430 0.15 -0.3
783,391 - BBR_RS13430 0.15 -1.4
783,428 - BBR_RS13430 0.21 -1.5
783,434 + BBR_RS13430 0.22 -2.3
783,444 - BBR_RS13430 0.23 -0.1
783,488 + BBR_RS13430 0.30 +0.2
783,488 + BBR_RS13430 0.30 +1.2
783,497 - BBR_RS13430 0.32 -1.3
783,513 + BBR_RS13430 0.34 +0.4
783,530 + BBR_RS13430 0.37 -0.1
783,533 - BBR_RS13430 0.37 +0.8
783,544 + BBR_RS13430 0.39 +0.1
783,544 + BBR_RS13430 0.39 -1.2
783,580 - BBR_RS13430 0.44 +0.3
783,593 + BBR_RS13430 0.46 +0.4
783,595 + BBR_RS13430 0.46 -0.4
783,598 + BBR_RS13430 0.47 -1.2
783,601 - BBR_RS13430 0.47 -0.4
783,601 - BBR_RS13430 0.47 +0.9
783,605 + BBR_RS13430 0.48 -0.3
783,700 - BBR_RS13430 0.62 +0.1
783,700 - BBR_RS13430 0.62 -1.0
783,751 + BBR_RS13430 0.70 -2.3
783,840 - BBR_RS13430 0.83 +0.0
783,857 + BBR_RS13430 0.86 -0.5
783,900 + -0.3
783,900 + -0.5
783,942 + -0.7
783,942 + +0.7
783,960 + -0.1
784,007 + -0.2
784,009 - -0.6
784,090 + BBR_RS13435 0.11 +0.5
784,174 - BBR_RS13435 0.17 -0.6
784,187 + BBR_RS13435 0.18 -1.1
784,195 - BBR_RS13435 0.18 +0.5
784,243 + BBR_RS13435 0.22 -2.0
784,243 + BBR_RS13435 0.22 -0.5
784,248 + BBR_RS13435 0.22 -1.8
784,253 + BBR_RS13435 0.22 -0.0
784,256 - BBR_RS13435 0.23 -0.0
784,256 - BBR_RS13435 0.23 +0.0
784,256 - BBR_RS13435 0.23 -0.7
784,263 - BBR_RS13435 0.23 +0.3
784,310 - BBR_RS13435 0.27 -1.3
784,355 + BBR_RS13435 0.30 -0.3
784,364 - BBR_RS13435 0.31 -1.2
784,378 - BBR_RS13435 0.32 +1.1
784,422 - BBR_RS13435 0.35 +0.2
784,427 - BBR_RS13435 0.35 -0.2
784,431 + BBR_RS13435 0.35 -0.9
784,494 + BBR_RS13435 0.40 +0.2
784,502 - BBR_RS13435 0.41 -0.3
784,502 - BBR_RS13435 0.41 -0.0
784,502 - BBR_RS13435 0.41 +0.9
784,632 + BBR_RS13435 0.50 -0.8
784,658 + BBR_RS13435 0.52 +0.1
784,658 + BBR_RS13435 0.52 -0.6
784,664 - BBR_RS13435 0.52 -0.6
784,673 + BBR_RS13435 0.53 -0.4
784,698 + BBR_RS13435 0.55 +0.3
784,698 + BBR_RS13435 0.55 -0.2
784,706 - BBR_RS13435 0.56 -1.5
784,708 + BBR_RS13435 0.56 -0.4
784,845 + BBR_RS13435 0.66 +0.1
784,870 - BBR_RS13435 0.67 -1.1
784,872 + BBR_RS13435 0.68 -0.1
784,906 - BBR_RS13435 0.70 -0.7
784,915 + BBR_RS13435 0.71 -2.5
784,915 + BBR_RS13435 0.71 -1.7
784,923 + BBR_RS13435 0.71 -0.7
784,929 - BBR_RS13435 0.72 +0.3
784,933 + BBR_RS13435 0.72 -0.4
784,941 - BBR_RS13435 0.73 -1.8
784,941 - BBR_RS13435 0.73 -0.8
784,941 - BBR_RS13435 0.73 -2.0
784,943 + BBR_RS13435 0.73 +0.3
785,110 - BBR_RS13435 0.85 -0.8
785,110 - BBR_RS13435 0.85 -1.1
785,116 - BBR_RS13435 0.85 -0.8
785,193 + -0.6
785,201 - -1.1
785,257 + -1.1
785,266 + +0.1
785,266 + -0.1
785,266 + -0.5
785,274 - -1.5
785,279 - -1.0
785,300 + +0.6
785,300 + -1.8
785,300 + +0.3
785,334 + -1.6
785,334 + -0.8
785,417 + -0.0
785,417 + -0.1
785,481 - BBR_RS13440 0.14 +0.4
785,493 - BBR_RS13440 0.15 -1.6
785,539 - BBR_RS13440 0.19 +0.5
785,546 + BBR_RS13440 0.19 -0.6
785,546 + BBR_RS13440 0.19 -1.5
785,554 - BBR_RS13440 0.20 -0.8
785,556 + BBR_RS13440 0.20 -0.0
785,560 + BBR_RS13440 0.20 -0.7
785,564 - BBR_RS13440 0.21 -0.4
785,568 - BBR_RS13440 0.21 -0.2
785,568 - BBR_RS13440 0.21 +0.1
785,568 - BBR_RS13440 0.21 +0.1
785,583 - BBR_RS13440 0.22 +0.8
785,587 + BBR_RS13440 0.22 -0.1
785,587 + BBR_RS13440 0.22 +0.1
785,587 + BBR_RS13440 0.22 +0.0
785,587 + BBR_RS13440 0.22 -0.6
785,595 - BBR_RS13440 0.23 -0.3
785,595 - BBR_RS13440 0.23 +0.1
785,650 + BBR_RS13440 0.27 -1.4
785,653 - BBR_RS13440 0.27 -0.1
785,667 - BBR_RS13440 0.28 -0.7
785,669 + BBR_RS13440 0.28 -1.0
785,672 - BBR_RS13440 0.29 -0.3
785,672 - BBR_RS13440 0.29 -0.8
785,672 - BBR_RS13440 0.29 -1.4
785,677 - BBR_RS13440 0.29 -0.3
785,677 - BBR_RS13440 0.29 -0.8
785,712 + BBR_RS13440 0.32 +0.1
785,712 + BBR_RS13440 0.32 +0.7
785,726 + BBR_RS13440 0.33 -1.0
785,733 + BBR_RS13440 0.33 -0.7
785,734 + BBR_RS13440 0.33 -0.5
785,734 + BBR_RS13440 0.33 -0.0
785,747 + BBR_RS13440 0.34 +0.2
785,769 + BBR_RS13440 0.36 -1.3
785,883 + BBR_RS13440 0.44 -0.2
785,934 - BBR_RS13440 0.48 -0.3
786,057 - BBR_RS13440 0.57 -0.4
786,064 + BBR_RS13440 0.58 -0.7
786,069 + BBR_RS13440 0.58 -1.0
786,101 + BBR_RS13440 0.61 +0.6
786,109 - BBR_RS13440 0.61 -0.7
786,177 + BBR_RS13440 0.66 +0.5
786,208 + BBR_RS13440 0.69 -0.5
786,216 - BBR_RS13440 0.69 -0.4
786,216 - BBR_RS13440 0.69 -0.2
786,221 - BBR_RS13440 0.70 -0.4
786,221 - BBR_RS13440 0.70 -0.7
786,230 - BBR_RS13440 0.70 -0.6
786,308 + BBR_RS13440 0.76 -1.2
786,314 + BBR_RS13440 0.77 +0.2

Or see this region's nucleotide sequence