Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13370 and BBR_RS13375 are separated by 26 nucleotides BBR_RS13375 and BBR_RS13380 are separated by 116 nucleotides
BBR_RS13370: BBR_RS13370 - phosphoribosylamine--glycine ligase, at 772,512 to 773,780
_RS13370
BBR_RS13375: BBR_RS13375 - phosphoribosylformylglycinamidine cyclo-ligase, at 773,807 to 774,844
_RS13375
BBR_RS13380: BBR_RS13380 - amidophosphoribosyltransferase, at 774,961 to 776,472
_RS13380
Position (kb)
773
774
775 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 773.309 kb on + strand, within BBR_RS13370 at 773.415 kb on - strand, within BBR_RS13370 at 773.415 kb on - strand, within BBR_RS13370 at 773.590 kb on - strand, within BBR_RS13370 at 773.594 kb on + strand, within BBR_RS13370 at 773.620 kb on - strand, within BBR_RS13370 at 773.644 kb on - strand, within BBR_RS13370 at 773.710 kb on + strand at 773.854 kb on - strand at 773.932 kb on - strand, within BBR_RS13375 at 773.932 kb on - strand, within BBR_RS13375 at 773.966 kb on - strand, within BBR_RS13375 at 774.101 kb on + strand, within BBR_RS13375 at 774.122 kb on - strand, within BBR_RS13375 at 774.124 kb on + strand, within BBR_RS13375 at 774.137 kb on - strand, within BBR_RS13375 at 774.137 kb on - strand, within BBR_RS13375 at 774.164 kb on - strand, within BBR_RS13375 at 774.166 kb on + strand, within BBR_RS13375 at 774.232 kb on - strand, within BBR_RS13375 at 774.256 kb on - strand, within BBR_RS13375 at 774.284 kb on - strand, within BBR_RS13375 at 774.284 kb on - strand, within BBR_RS13375 at 774.435 kb on + strand, within BBR_RS13375 at 774.453 kb on - strand, within BBR_RS13375 at 774.491 kb on - strand, within BBR_RS13375 at 774.558 kb on - strand, within BBR_RS13375 at 774.679 kb on - strand, within BBR_RS13375 at 774.884 kb on - strand at 774.935 kb on + strand at 774.968 kb on - strand at 774.983 kb on + strand at 774.991 kb on - strand at 774.991 kb on - strand at 774.991 kb on - strand at 774.991 kb on - strand at 774.991 kb on - strand at 774.991 kb on - strand at 774.991 kb on - strand at 774.993 kb on - strand at 775.010 kb on + strand at 775.018 kb on - strand at 775.018 kb on - strand at 775.018 kb on - strand at 775.018 kb on - strand at 775.033 kb on - strand at 775.035 kb on - strand at 775.043 kb on - strand at 775.053 kb on - strand at 775.053 kb on - strand at 775.053 kb on - strand at 775.053 kb on - strand at 775.061 kb on - strand at 775.143 kb on - strand, within BBR_RS13380 at 775.248 kb on - strand, within BBR_RS13380 at 775.326 kb on - strand, within BBR_RS13380 at 775.424 kb on + strand, within BBR_RS13380 at 775.426 kb on - strand, within BBR_RS13380 at 775.431 kb on - strand, within BBR_RS13380 at 775.564 kb on - strand, within BBR_RS13380 at 775.575 kb on - strand, within BBR_RS13380 at 775.599 kb on - strand, within BBR_RS13380 at 775.682 kb on - strand, within BBR_RS13380 at 775.717 kb on - strand, within BBR_RS13380 at 775.717 kb on - strand, within BBR_RS13380 at 775.750 kb on + strand, within BBR_RS13380 at 775.759 kb on + strand, within BBR_RS13380 at 775.814 kb on - strand, within BBR_RS13380 at 775.827 kb on - strand, within BBR_RS13380 at 775.832 kb on - strand, within BBR_RS13380 at 775.837 kb on - strand, within BBR_RS13380
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 773,309 + BBR_RS13370 0.63 +0.7 773,415 - BBR_RS13370 0.71 -2.3 773,415 - BBR_RS13370 0.71 -1.9 773,590 - BBR_RS13370 0.85 -0.7 773,594 + BBR_RS13370 0.85 -0.7 773,620 - BBR_RS13370 0.87 -0.7 773,644 - BBR_RS13370 0.89 +1.7 773,710 + +0.4 773,854 - -0.3 773,932 - BBR_RS13375 0.12 -0.2 773,932 - BBR_RS13375 0.12 +0.6 773,966 - BBR_RS13375 0.15 -0.5 774,101 + BBR_RS13375 0.28 +3.3 774,122 - BBR_RS13375 0.30 -0.4 774,124 + BBR_RS13375 0.31 +0.7 774,137 - BBR_RS13375 0.32 -2.5 774,137 - BBR_RS13375 0.32 -2.0 774,164 - BBR_RS13375 0.34 -0.2 774,166 + BBR_RS13375 0.35 -0.4 774,232 - BBR_RS13375 0.41 -1.7 774,256 - BBR_RS13375 0.43 -2.0 774,284 - BBR_RS13375 0.46 -1.4 774,284 - BBR_RS13375 0.46 -1.7 774,435 + BBR_RS13375 0.61 -0.7 774,453 - BBR_RS13375 0.62 +0.6 774,491 - BBR_RS13375 0.66 -1.0 774,558 - BBR_RS13375 0.72 -1.5 774,679 - BBR_RS13375 0.84 -0.3 774,884 - -0.2 774,935 + +1.7 774,968 - +0.3 774,983 + +1.4 774,991 - -1.7 774,991 - +0.0 774,991 - -0.3 774,991 - +0.2 774,991 - -0.6 774,991 - -0.6 774,991 - -0.9 774,993 - -0.5 775,010 + +0.7 775,018 - +1.3 775,018 - -0.4 775,018 - -1.0 775,018 - -0.6 775,033 - -1.3 775,035 - -0.4 775,043 - -1.5 775,053 - -0.4 775,053 - -1.2 775,053 - -3.1 775,053 - -0.6 775,061 - +0.4 775,143 - BBR_RS13380 0.12 -0.6 775,248 - BBR_RS13380 0.19 -0.9 775,326 - BBR_RS13380 0.24 -1.6 775,424 + BBR_RS13380 0.31 +1.7 775,426 - BBR_RS13380 0.31 +1.3 775,431 - BBR_RS13380 0.31 -1.3 775,564 - BBR_RS13380 0.40 +0.0 775,575 - BBR_RS13380 0.41 +0.7 775,599 - BBR_RS13380 0.42 -0.9 775,682 - BBR_RS13380 0.48 -0.8 775,717 - BBR_RS13380 0.50 -0.6 775,717 - BBR_RS13380 0.50 +0.1 775,750 + BBR_RS13380 0.52 -0.6 775,759 + BBR_RS13380 0.53 +1.7 775,814 - BBR_RS13380 0.56 +0.3 775,827 - BBR_RS13380 0.57 -1.0 775,832 - BBR_RS13380 0.58 +0.9 775,837 - BBR_RS13380 0.58 -1.6
Or see this region's nucleotide sequence