Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13300 and BBR_RS13305 are separated by 8 nucleotides BBR_RS13305 and BBR_RS13310 are separated by 46 nucleotides BBR_RS13310 and BBR_RS13315 overlap by 4 nucleotides
BBR_RS13300: BBR_RS13300 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 759,094 to 760,272
_RS13300
BBR_RS13305: BBR_RS13305 - transcriptional repressor, at 760,281 to 760,709
_RS13305
BBR_RS13310: BBR_RS13310 - hypothetical protein, at 760,756 to 761,466
_RS13310
BBR_RS13315: BBR_RS13315 - hypothetical protein, at 761,463 to 761,882
_RS13315
Position (kb) 760
761 Strain fitness (log2 ratio) -2
-1
0
1 at 759.413 kb on + strand, within BBR_RS13300 at 759.498 kb on - strand, within BBR_RS13300 at 759.595 kb on - strand, within BBR_RS13300 at 760.274 kb on - strand at 760.281 kb on - strand at 760.337 kb on + strand, within BBR_RS13305 at 760.342 kb on + strand, within BBR_RS13305 at 760.350 kb on - strand, within BBR_RS13305 at 760.350 kb on - strand, within BBR_RS13305 at 760.384 kb on - strand, within BBR_RS13305 at 760.518 kb on + strand, within BBR_RS13305 at 760.602 kb on + strand, within BBR_RS13305 at 760.607 kb on + strand, within BBR_RS13305 at 760.630 kb on + strand, within BBR_RS13305 at 760.630 kb on + strand, within BBR_RS13305 at 760.632 kb on + strand, within BBR_RS13305 at 760.638 kb on - strand, within BBR_RS13305 at 760.638 kb on - strand, within BBR_RS13305 at 760.638 kb on - strand, within BBR_RS13305 at 760.638 kb on - strand, within BBR_RS13305 at 760.685 kb on - strand at 760.689 kb on + strand at 760.712 kb on - strand at 760.755 kb on + strand at 760.758 kb on + strand at 760.758 kb on + strand at 760.793 kb on + strand at 760.793 kb on + strand at 760.793 kb on + strand at 760.793 kb on + strand at 760.818 kb on + strand at 760.836 kb on + strand, within BBR_RS13310 at 760.907 kb on + strand, within BBR_RS13310 at 760.937 kb on + strand, within BBR_RS13310 at 760.945 kb on - strand, within BBR_RS13310 at 761.001 kb on - strand, within BBR_RS13310 at 761.006 kb on - strand, within BBR_RS13310 at 761.031 kb on + strand, within BBR_RS13310 at 761.046 kb on + strand, within BBR_RS13310 at 761.054 kb on - strand, within BBR_RS13310 at 761.082 kb on + strand, within BBR_RS13310 at 761.082 kb on + strand, within BBR_RS13310 at 761.082 kb on + strand, within BBR_RS13310 at 761.082 kb on + strand, within BBR_RS13310 at 761.090 kb on - strand, within BBR_RS13310 at 761.090 kb on - strand, within BBR_RS13310 at 761.109 kb on - strand, within BBR_RS13310 at 761.188 kb on + strand, within BBR_RS13310 at 761.196 kb on + strand, within BBR_RS13310 at 761.198 kb on - strand, within BBR_RS13310 at 761.198 kb on - strand, within BBR_RS13310 at 761.206 kb on + strand, within BBR_RS13310 at 761.207 kb on - strand, within BBR_RS13310 at 761.291 kb on + strand, within BBR_RS13310 at 761.336 kb on - strand, within BBR_RS13310 at 761.338 kb on - strand, within BBR_RS13310 at 761.363 kb on + strand, within BBR_RS13310 at 761.377 kb on + strand, within BBR_RS13310 at 761.421 kb on + strand at 761.453 kb on + strand at 761.458 kb on + strand at 761.459 kb on + strand at 761.461 kb on - strand at 761.468 kb on + strand at 761.509 kb on + strand, within BBR_RS13315 at 761.522 kb on - strand, within BBR_RS13315 at 761.527 kb on - strand, within BBR_RS13315 at 761.527 kb on - strand, within BBR_RS13315 at 761.532 kb on + strand, within BBR_RS13315 at 761.551 kb on + strand, within BBR_RS13315 at 761.569 kb on - strand, within BBR_RS13315 at 761.569 kb on - strand, within BBR_RS13315 at 761.668 kb on + strand, within BBR_RS13315 at 761.670 kb on - strand, within BBR_RS13315 at 761.674 kb on + strand, within BBR_RS13315 at 761.702 kb on - strand, within BBR_RS13315 at 761.702 kb on - strand, within BBR_RS13315
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 759,413 + BBR_RS13300 0.27 +0.5 759,498 - BBR_RS13300 0.34 +0.7 759,595 - BBR_RS13300 0.42 +0.2 760,274 - -0.2 760,281 - +0.4 760,337 + BBR_RS13305 0.13 +0.0 760,342 + BBR_RS13305 0.14 +0.4 760,350 - BBR_RS13305 0.16 -1.5 760,350 - BBR_RS13305 0.16 -1.3 760,384 - BBR_RS13305 0.24 -1.3 760,518 + BBR_RS13305 0.55 -1.5 760,602 + BBR_RS13305 0.75 -1.4 760,607 + BBR_RS13305 0.76 -2.0 760,630 + BBR_RS13305 0.81 -1.1 760,630 + BBR_RS13305 0.81 -0.6 760,632 + BBR_RS13305 0.82 -1.1 760,638 - BBR_RS13305 0.83 -0.1 760,638 - BBR_RS13305 0.83 +0.0 760,638 - BBR_RS13305 0.83 -0.4 760,638 - BBR_RS13305 0.83 -0.4 760,685 - -0.1 760,689 + -0.2 760,712 - -1.3 760,755 + -0.7 760,758 + +0.1 760,758 + -0.2 760,793 + -0.8 760,793 + -1.0 760,793 + -0.3 760,793 + -0.1 760,818 + -0.6 760,836 + BBR_RS13310 0.11 -0.6 760,907 + BBR_RS13310 0.21 +0.2 760,937 + BBR_RS13310 0.25 -0.3 760,945 - BBR_RS13310 0.27 -0.3 761,001 - BBR_RS13310 0.34 +0.4 761,006 - BBR_RS13310 0.35 -0.5 761,031 + BBR_RS13310 0.39 +0.4 761,046 + BBR_RS13310 0.41 -1.3 761,054 - BBR_RS13310 0.42 -0.2 761,082 + BBR_RS13310 0.46 -1.5 761,082 + BBR_RS13310 0.46 +0.2 761,082 + BBR_RS13310 0.46 +0.4 761,082 + BBR_RS13310 0.46 -0.6 761,090 - BBR_RS13310 0.47 -0.3 761,090 - BBR_RS13310 0.47 +0.8 761,109 - BBR_RS13310 0.50 -0.6 761,188 + BBR_RS13310 0.61 -0.0 761,196 + BBR_RS13310 0.62 +0.7 761,198 - BBR_RS13310 0.62 +0.3 761,198 - BBR_RS13310 0.62 +0.6 761,206 + BBR_RS13310 0.63 -1.0 761,207 - BBR_RS13310 0.63 -0.4 761,291 + BBR_RS13310 0.75 -0.1 761,336 - BBR_RS13310 0.82 +0.4 761,338 - BBR_RS13310 0.82 +0.3 761,363 + BBR_RS13310 0.85 +0.3 761,377 + BBR_RS13310 0.87 +0.7 761,421 + -1.4 761,453 + -0.8 761,458 + +0.6 761,459 + -0.8 761,461 - -1.2 761,468 + +0.2 761,509 + BBR_RS13315 0.11 -0.2 761,522 - BBR_RS13315 0.14 +0.5 761,527 - BBR_RS13315 0.15 +0.1 761,527 - BBR_RS13315 0.15 +1.0 761,532 + BBR_RS13315 0.16 -0.0 761,551 + BBR_RS13315 0.21 +1.1 761,569 - BBR_RS13315 0.25 +1.6 761,569 - BBR_RS13315 0.25 -0.6 761,668 + BBR_RS13315 0.49 +1.5 761,670 - BBR_RS13315 0.49 -0.7 761,674 + BBR_RS13315 0.50 -0.3 761,702 - BBR_RS13315 0.57 -0.8 761,702 - BBR_RS13315 0.57 -0.8
Or see this region's nucleotide sequence