Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20525 and BBR_RS12805 are separated by 109 nucleotides BBR_RS12805 and BBR_RS12810 are separated by 166 nucleotides BBR_RS12810 and BBR_RS12815 are separated by 306 nucleotides
BBR_RS20525: BBR_RS20525 - DUF3800 domain-containing protein, at 621,501 to 622,313
_RS20525
BBR_RS12805: BBR_RS12805 - hypothetical protein, at 622,423 to 622,620
_RS12805
BBR_RS12810: BBR_RS12810 - hypothetical protein, at 622,787 to 624,178
_RS12810
BBR_RS12815: BBR_RS12815 - hypothetical protein, at 624,485 to 624,919
_RS12815
Position (kb)
622
623
624
625 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 621.899 kb on + strand, within BBR_RS20525 at 621.912 kb on - strand, within BBR_RS20525 at 621.930 kb on + strand, within BBR_RS20525 at 621.932 kb on + strand, within BBR_RS20525 at 621.938 kb on - strand, within BBR_RS20525 at 621.940 kb on - strand, within BBR_RS20525 at 621.950 kb on - strand, within BBR_RS20525 at 621.954 kb on - strand, within BBR_RS20525 at 622.045 kb on + strand, within BBR_RS20525 at 622.112 kb on + strand, within BBR_RS20525 at 622.174 kb on - strand, within BBR_RS20525 at 622.204 kb on - strand, within BBR_RS20525 at 622.269 kb on + strand at 622.269 kb on + strand at 622.277 kb on + strand at 622.289 kb on + strand at 622.372 kb on - strand at 622.410 kb on - strand at 622.437 kb on + strand at 622.468 kb on - strand, within BBR_RS12805 at 622.470 kb on + strand, within BBR_RS12805 at 622.563 kb on + strand, within BBR_RS12805 at 622.563 kb on + strand, within BBR_RS12805 at 622.607 kb on - strand at 622.632 kb on - strand at 622.749 kb on - strand at 622.759 kb on - strand at 622.771 kb on - strand at 622.786 kb on + strand at 622.793 kb on - strand at 622.802 kb on - strand at 622.827 kb on + strand at 622.839 kb on + strand at 622.882 kb on + strand at 622.884 kb on + strand at 622.890 kb on + strand at 622.892 kb on - strand at 622.914 kb on + strand at 622.914 kb on + strand at 622.936 kb on - strand, within BBR_RS12810 at 622.936 kb on - strand, within BBR_RS12810 at 622.965 kb on - strand, within BBR_RS12810 at 623.015 kb on + strand, within BBR_RS12810 at 623.021 kb on + strand, within BBR_RS12810 at 623.094 kb on + strand, within BBR_RS12810 at 623.103 kb on - strand, within BBR_RS12810 at 623.125 kb on - strand, within BBR_RS12810 at 623.125 kb on - strand, within BBR_RS12810 at 623.132 kb on - strand, within BBR_RS12810 at 623.136 kb on + strand, within BBR_RS12810 at 623.136 kb on + strand, within BBR_RS12810 at 623.136 kb on + strand, within BBR_RS12810 at 623.198 kb on + strand, within BBR_RS12810 at 623.206 kb on - strand, within BBR_RS12810 at 623.206 kb on - strand, within BBR_RS12810 at 623.224 kb on + strand, within BBR_RS12810 at 623.224 kb on + strand, within BBR_RS12810 at 623.242 kb on - strand, within BBR_RS12810 at 623.277 kb on - strand, within BBR_RS12810 at 623.319 kb on - strand, within BBR_RS12810 at 623.326 kb on + strand, within BBR_RS12810 at 623.326 kb on + strand, within BBR_RS12810 at 623.334 kb on - strand, within BBR_RS12810 at 623.350 kb on + strand, within BBR_RS12810 at 623.350 kb on + strand, within BBR_RS12810 at 623.357 kb on - strand, within BBR_RS12810 at 623.393 kb on + strand, within BBR_RS12810 at 623.409 kb on + strand, within BBR_RS12810 at 623.409 kb on + strand, within BBR_RS12810 at 623.414 kb on + strand, within BBR_RS12810 at 623.417 kb on - strand, within BBR_RS12810 at 623.441 kb on - strand, within BBR_RS12810 at 623.497 kb on + strand, within BBR_RS12810 at 623.572 kb on - strand, within BBR_RS12810 at 623.591 kb on + strand, within BBR_RS12810 at 623.597 kb on + strand, within BBR_RS12810 at 623.655 kb on + strand, within BBR_RS12810 at 623.673 kb on + strand, within BBR_RS12810 at 623.678 kb on - strand, within BBR_RS12810 at 623.705 kb on - strand, within BBR_RS12810 at 623.719 kb on - strand, within BBR_RS12810 at 623.731 kb on - strand, within BBR_RS12810 at 623.731 kb on - strand, within BBR_RS12810 at 623.762 kb on - strand, within BBR_RS12810 at 623.781 kb on - strand, within BBR_RS12810 at 623.788 kb on - strand, within BBR_RS12810 at 623.819 kb on + strand, within BBR_RS12810 at 623.843 kb on + strand, within BBR_RS12810 at 623.853 kb on + strand, within BBR_RS12810 at 623.861 kb on - strand, within BBR_RS12810 at 623.917 kb on + strand, within BBR_RS12810 at 623.934 kb on + strand, within BBR_RS12810 at 623.934 kb on + strand, within BBR_RS12810 at 623.934 kb on + strand, within BBR_RS12810 at 623.946 kb on + strand, within BBR_RS12810 at 623.981 kb on - strand, within BBR_RS12810 at 624.023 kb on - strand, within BBR_RS12810 at 624.035 kb on + strand, within BBR_RS12810 at 624.052 kb on + strand at 624.144 kb on - strand at 624.177 kb on - strand at 624.179 kb on + strand at 624.187 kb on - strand at 624.191 kb on + strand at 624.191 kb on + strand at 624.191 kb on + strand at 624.199 kb on - strand at 624.199 kb on - strand at 624.199 kb on - strand at 624.199 kb on - strand at 624.227 kb on + strand at 624.233 kb on - strand at 624.233 kb on - strand at 624.243 kb on - strand at 624.257 kb on - strand at 624.283 kb on - strand at 624.285 kb on + strand at 624.293 kb on - strand at 624.361 kb on + strand at 624.369 kb on - strand at 624.369 kb on - strand at 624.373 kb on + strand at 624.455 kb on + strand at 624.455 kb on + strand at 624.455 kb on - strand at 624.492 kb on + strand at 624.493 kb on - strand at 624.519 kb on - strand at 624.519 kb on - strand at 624.520 kb on - strand at 624.528 kb on - strand at 624.557 kb on + strand, within BBR_RS12815 at 624.571 kb on + strand, within BBR_RS12815 at 624.571 kb on + strand, within BBR_RS12815 at 624.599 kb on + strand, within BBR_RS12815 at 624.632 kb on - strand, within BBR_RS12815 at 624.670 kb on + strand, within BBR_RS12815 at 624.672 kb on - strand, within BBR_RS12815 at 624.693 kb on + strand, within BBR_RS12815 at 624.696 kb on - strand, within BBR_RS12815 at 624.700 kb on - strand, within BBR_RS12815 at 624.701 kb on - strand, within BBR_RS12815 at 624.701 kb on - strand, within BBR_RS12815 at 624.701 kb on - strand, within BBR_RS12815 at 624.717 kb on + strand, within BBR_RS12815 at 624.725 kb on - strand, within BBR_RS12815 at 624.735 kb on - strand, within BBR_RS12815 at 624.761 kb on + strand, within BBR_RS12815 at 624.788 kb on - strand, within BBR_RS12815 at 624.852 kb on - strand, within BBR_RS12815 at 624.859 kb on - strand, within BBR_RS12815 at 624.873 kb on + strand, within BBR_RS12815 at 624.873 kb on + strand, within BBR_RS12815 at 624.873 kb on + strand, within BBR_RS12815 at 624.881 kb on - strand at 624.881 kb on - strand at 624.899 kb on - strand at 624.928 kb on + strand at 624.936 kb on - strand at 624.936 kb on - strand at 625.094 kb on + strand at 625.095 kb on - strand at 625.095 kb on - strand at 625.100 kb on - strand at 625.117 kb on - strand at 625.137 kb on + strand at 625.148 kb on + strand at 625.168 kb on + strand at 625.168 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 621,899 + BBR_RS20525 0.49 -0.4 621,912 - BBR_RS20525 0.51 +0.4 621,930 + BBR_RS20525 0.53 -0.8 621,932 + BBR_RS20525 0.53 -0.2 621,938 - BBR_RS20525 0.54 +2.2 621,940 - BBR_RS20525 0.54 +0.3 621,950 - BBR_RS20525 0.55 -1.2 621,954 - BBR_RS20525 0.56 -0.9 622,045 + BBR_RS20525 0.67 -0.6 622,112 + BBR_RS20525 0.75 +1.9 622,174 - BBR_RS20525 0.83 +1.1 622,204 - BBR_RS20525 0.86 +0.6 622,269 + -1.2 622,269 + -0.1 622,277 + -0.0 622,289 + -0.3 622,372 - +0.3 622,410 - +0.6 622,437 + -1.1 622,468 - BBR_RS12805 0.23 +0.6 622,470 + BBR_RS12805 0.24 -0.4 622,563 + BBR_RS12805 0.71 -0.4 622,563 + BBR_RS12805 0.71 -0.0 622,607 - +0.3 622,632 - +0.2 622,749 - +0.8 622,759 - +0.2 622,771 - -0.6 622,786 + +0.2 622,793 - -0.4 622,802 - -0.9 622,827 + -0.8 622,839 + -1.6 622,882 + -0.3 622,884 + -0.2 622,890 + -2.1 622,892 - -0.2 622,914 + -0.3 622,914 + -1.1 622,936 - BBR_RS12810 0.11 -0.6 622,936 - BBR_RS12810 0.11 -1.2 622,965 - BBR_RS12810 0.13 -0.9 623,015 + BBR_RS12810 0.16 +1.6 623,021 + BBR_RS12810 0.17 -1.0 623,094 + BBR_RS12810 0.22 +1.2 623,103 - BBR_RS12810 0.23 -0.6 623,125 - BBR_RS12810 0.24 +0.3 623,125 - BBR_RS12810 0.24 -2.3 623,132 - BBR_RS12810 0.25 +1.3 623,136 + BBR_RS12810 0.25 -0.5 623,136 + BBR_RS12810 0.25 -1.1 623,136 + BBR_RS12810 0.25 -0.7 623,198 + BBR_RS12810 0.30 -0.5 623,206 - BBR_RS12810 0.30 -2.8 623,206 - BBR_RS12810 0.30 -0.5 623,224 + BBR_RS12810 0.31 -0.4 623,224 + BBR_RS12810 0.31 -0.9 623,242 - BBR_RS12810 0.33 -0.7 623,277 - BBR_RS12810 0.35 -2.8 623,319 - BBR_RS12810 0.38 -0.7 623,326 + BBR_RS12810 0.39 +0.4 623,326 + BBR_RS12810 0.39 +0.9 623,334 - BBR_RS12810 0.39 +0.1 623,350 + BBR_RS12810 0.40 -1.2 623,350 + BBR_RS12810 0.40 -0.4 623,357 - BBR_RS12810 0.41 +1.8 623,393 + BBR_RS12810 0.44 +0.6 623,409 + BBR_RS12810 0.45 +0.1 623,409 + BBR_RS12810 0.45 -0.6 623,414 + BBR_RS12810 0.45 +0.1 623,417 - BBR_RS12810 0.45 -0.4 623,441 - BBR_RS12810 0.47 -0.3 623,497 + BBR_RS12810 0.51 -1.3 623,572 - BBR_RS12810 0.56 -1.9 623,591 + BBR_RS12810 0.58 -1.1 623,597 + BBR_RS12810 0.58 +0.1 623,655 + BBR_RS12810 0.62 +0.3 623,673 + BBR_RS12810 0.64 -0.9 623,678 - BBR_RS12810 0.64 -1.4 623,705 - BBR_RS12810 0.66 -1.4 623,719 - BBR_RS12810 0.67 -0.1 623,731 - BBR_RS12810 0.68 -2.1 623,731 - BBR_RS12810 0.68 -0.5 623,762 - BBR_RS12810 0.70 -0.7 623,781 - BBR_RS12810 0.71 -1.2 623,788 - BBR_RS12810 0.72 -1.2 623,819 + BBR_RS12810 0.74 -0.6 623,843 + BBR_RS12810 0.76 -0.9 623,853 + BBR_RS12810 0.77 -0.5 623,861 - BBR_RS12810 0.77 -0.7 623,917 + BBR_RS12810 0.81 -0.7 623,934 + BBR_RS12810 0.82 -1.6 623,934 + BBR_RS12810 0.82 -0.8 623,934 + BBR_RS12810 0.82 +1.5 623,946 + BBR_RS12810 0.83 +0.3 623,981 - BBR_RS12810 0.86 -0.2 624,023 - BBR_RS12810 0.89 -1.2 624,035 + BBR_RS12810 0.90 +0.0 624,052 + +1.2 624,144 - -1.1 624,177 - -1.0 624,179 + -0.7 624,187 - +1.8 624,191 + -0.5 624,191 + -0.4 624,191 + -1.0 624,199 - +0.4 624,199 - +0.1 624,199 - +0.8 624,199 - +1.9 624,227 + -0.1 624,233 - +1.5 624,233 - -1.2 624,243 - +2.8 624,257 - -0.2 624,283 - +1.0 624,285 + +1.2 624,293 - +1.0 624,361 + -0.1 624,369 - -1.3 624,369 - -0.1 624,373 + -1.0 624,455 + +0.4 624,455 + +0.3 624,455 - -0.3 624,492 + +1.1 624,493 - -0.0 624,519 - +1.4 624,519 - +0.2 624,520 - -0.7 624,528 - -0.6 624,557 + BBR_RS12815 0.17 -0.5 624,571 + BBR_RS12815 0.20 +0.5 624,571 + BBR_RS12815 0.20 -0.2 624,599 + BBR_RS12815 0.26 +0.2 624,632 - BBR_RS12815 0.34 -0.6 624,670 + BBR_RS12815 0.43 +1.3 624,672 - BBR_RS12815 0.43 -0.3 624,693 + BBR_RS12815 0.48 +0.7 624,696 - BBR_RS12815 0.49 -0.2 624,700 - BBR_RS12815 0.49 -1.8 624,701 - BBR_RS12815 0.50 +0.1 624,701 - BBR_RS12815 0.50 +0.0 624,701 - BBR_RS12815 0.50 -0.6 624,717 + BBR_RS12815 0.53 +1.2 624,725 - BBR_RS12815 0.55 -0.7 624,735 - BBR_RS12815 0.57 -0.3 624,761 + BBR_RS12815 0.63 +1.2 624,788 - BBR_RS12815 0.70 -1.0 624,852 - BBR_RS12815 0.84 -1.3 624,859 - BBR_RS12815 0.86 +0.6 624,873 + BBR_RS12815 0.89 -0.4 624,873 + BBR_RS12815 0.89 -0.6 624,873 + BBR_RS12815 0.89 +0.0 624,881 - -0.2 624,881 - -0.2 624,899 - +1.0 624,928 + +0.7 624,936 - -1.2 624,936 - -0.0 625,094 + -1.3 625,095 - +1.9 625,095 - -0.5 625,100 - +0.0 625,117 - -0.3 625,137 + +0.2 625,148 + -0.0 625,168 + +2.9 625,168 + +0.7
Or see this region's nucleotide sequence