Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12495 and BBR_RS12500 are separated by 235 nucleotides BBR_RS12500 and BBR_RS12505 are separated by 52 nucleotides
BBR_RS12495: BBR_RS12495 - sugar ABC transporter substrate-binding protein, at 555,379 to 556,728
_RS12495
BBR_RS12500: BBR_RS12500 - sugar ABC transporter permease, at 556,964 to 557,911
_RS12500
BBR_RS12505: BBR_RS12505 - ABC transporter permease, at 557,964 to 558,962
_RS12505
Position (kb)
556
557
558 Strain fitness (log2 ratio)
-2
-1
0
1 at 555.969 kb on + strand, within BBR_RS12495 at 555.977 kb on + strand, within BBR_RS12495 at 555.984 kb on + strand, within BBR_RS12495 at 555.994 kb on + strand, within BBR_RS12495 at 555.999 kb on + strand, within BBR_RS12495 at 556.002 kb on - strand, within BBR_RS12495 at 556.002 kb on - strand, within BBR_RS12495 at 556.003 kb on + strand, within BBR_RS12495 at 556.003 kb on + strand, within BBR_RS12495 at 556.021 kb on - strand, within BBR_RS12495 at 556.021 kb on - strand, within BBR_RS12495 at 556.021 kb on - strand, within BBR_RS12495 at 556.028 kb on - strand, within BBR_RS12495 at 556.044 kb on + strand, within BBR_RS12495 at 556.065 kb on + strand, within BBR_RS12495 at 556.065 kb on + strand, within BBR_RS12495 at 556.065 kb on + strand, within BBR_RS12495 at 556.067 kb on - strand, within BBR_RS12495 at 556.067 kb on - strand, within BBR_RS12495 at 556.067 kb on - strand, within BBR_RS12495 at 556.105 kb on + strand, within BBR_RS12495 at 556.105 kb on + strand, within BBR_RS12495 at 556.118 kb on + strand, within BBR_RS12495 at 556.128 kb on + strand, within BBR_RS12495 at 556.128 kb on + strand, within BBR_RS12495 at 556.128 kb on - strand, within BBR_RS12495 at 556.135 kb on + strand, within BBR_RS12495 at 556.136 kb on - strand, within BBR_RS12495 at 556.145 kb on + strand, within BBR_RS12495 at 556.145 kb on + strand, within BBR_RS12495 at 556.145 kb on + strand, within BBR_RS12495 at 556.153 kb on + strand, within BBR_RS12495 at 556.153 kb on + strand, within BBR_RS12495 at 556.153 kb on - strand, within BBR_RS12495 at 556.153 kb on - strand, within BBR_RS12495 at 556.160 kb on + strand, within BBR_RS12495 at 556.189 kb on + strand, within BBR_RS12495 at 556.243 kb on - strand, within BBR_RS12495 at 556.243 kb on - strand, within BBR_RS12495 at 556.341 kb on + strand, within BBR_RS12495 at 556.355 kb on - strand, within BBR_RS12495 at 556.360 kb on + strand, within BBR_RS12495 at 556.377 kb on + strand, within BBR_RS12495 at 556.377 kb on + strand, within BBR_RS12495 at 556.377 kb on + strand, within BBR_RS12495 at 556.377 kb on + strand, within BBR_RS12495 at 556.377 kb on + strand, within BBR_RS12495 at 556.385 kb on - strand, within BBR_RS12495 at 556.389 kb on + strand, within BBR_RS12495 at 556.389 kb on + strand, within BBR_RS12495 at 556.397 kb on - strand, within BBR_RS12495 at 556.399 kb on + strand, within BBR_RS12495 at 556.399 kb on + strand, within BBR_RS12495 at 556.412 kb on - strand, within BBR_RS12495 at 556.416 kb on + strand, within BBR_RS12495 at 556.426 kb on - strand, within BBR_RS12495 at 556.454 kb on - strand, within BBR_RS12495 at 556.503 kb on - strand, within BBR_RS12495 at 556.558 kb on + strand, within BBR_RS12495 at 556.586 kb on - strand, within BBR_RS12495 at 556.594 kb on + strand at 556.595 kb on - strand at 556.610 kb on + strand at 556.610 kb on + strand at 556.620 kb on + strand at 556.626 kb on + strand at 556.626 kb on + strand at 556.626 kb on - strand at 556.634 kb on - strand at 556.634 kb on - strand at 556.634 kb on - strand at 556.636 kb on + strand at 556.644 kb on - strand at 556.654 kb on - strand at 556.658 kb on - strand at 556.660 kb on + strand at 556.660 kb on + strand at 556.660 kb on + strand at 556.660 kb on + strand at 556.660 kb on + strand at 556.660 kb on + strand at 556.660 kb on + strand at 556.660 kb on + strand at 556.668 kb on - strand at 556.668 kb on - strand at 556.668 kb on - strand at 556.668 kb on - strand at 556.668 kb on - strand at 556.706 kb on + strand at 556.706 kb on - strand at 556.712 kb on - strand at 556.712 kb on - strand at 556.712 kb on - strand at 556.714 kb on - strand at 556.757 kb on - strand at 556.758 kb on + strand at 556.758 kb on + strand at 556.761 kb on - strand at 556.766 kb on - strand at 556.771 kb on + strand at 556.805 kb on - strand at 556.805 kb on - strand at 556.818 kb on + strand at 556.824 kb on - strand at 556.826 kb on - strand at 556.842 kb on - strand at 556.850 kb on - strand at 556.855 kb on + strand at 556.860 kb on + strand at 556.863 kb on - strand at 556.863 kb on - strand at 556.863 kb on - strand at 556.865 kb on + strand at 556.873 kb on - strand at 556.878 kb on - strand at 556.897 kb on + strand at 556.903 kb on - strand at 556.905 kb on - strand at 556.909 kb on - strand at 556.922 kb on - strand at 556.997 kb on - strand at 557.012 kb on + strand at 557.012 kb on + strand at 557.012 kb on + strand at 557.024 kb on + strand at 557.024 kb on + strand at 557.024 kb on + strand at 557.024 kb on + strand at 557.024 kb on + strand at 557.024 kb on + strand at 557.024 kb on + strand at 557.024 kb on + strand at 557.024 kb on + strand at 557.024 kb on + strand at 557.025 kb on - strand at 557.032 kb on - strand at 557.052 kb on - strand at 557.052 kb on - strand at 557.081 kb on - strand, within BBR_RS12500 at 558.799 kb on + strand, within BBR_RS12505 at 558.823 kb on + strand, within BBR_RS12505
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 555,969 + BBR_RS12495 0.44 -0.9 555,977 + BBR_RS12495 0.44 +0.6 555,984 + BBR_RS12495 0.45 +0.2 555,994 + BBR_RS12495 0.46 -1.8 555,999 + BBR_RS12495 0.46 -0.1 556,002 - BBR_RS12495 0.46 -0.8 556,002 - BBR_RS12495 0.46 -0.7 556,003 + BBR_RS12495 0.46 +0.4 556,003 + BBR_RS12495 0.46 -0.4 556,021 - BBR_RS12495 0.48 -0.6 556,021 - BBR_RS12495 0.48 -0.8 556,021 - BBR_RS12495 0.48 -0.5 556,028 - BBR_RS12495 0.48 +0.4 556,044 + BBR_RS12495 0.49 +0.7 556,065 + BBR_RS12495 0.51 +0.3 556,065 + BBR_RS12495 0.51 -0.4 556,065 + BBR_RS12495 0.51 +0.4 556,067 - BBR_RS12495 0.51 -2.0 556,067 - BBR_RS12495 0.51 +1.0 556,067 - BBR_RS12495 0.51 +0.1 556,105 + BBR_RS12495 0.54 +0.3 556,105 + BBR_RS12495 0.54 -0.1 556,118 + BBR_RS12495 0.55 -0.3 556,128 + BBR_RS12495 0.55 -0.8 556,128 + BBR_RS12495 0.55 -0.2 556,128 - BBR_RS12495 0.55 -0.4 556,135 + BBR_RS12495 0.56 +0.4 556,136 - BBR_RS12495 0.56 +1.0 556,145 + BBR_RS12495 0.57 +0.5 556,145 + BBR_RS12495 0.57 +0.6 556,145 + BBR_RS12495 0.57 -0.2 556,153 + BBR_RS12495 0.57 -1.2 556,153 + BBR_RS12495 0.57 +1.0 556,153 - BBR_RS12495 0.57 -1.0 556,153 - BBR_RS12495 0.57 -1.1 556,160 + BBR_RS12495 0.58 +1.0 556,189 + BBR_RS12495 0.60 +0.4 556,243 - BBR_RS12495 0.64 +0.2 556,243 - BBR_RS12495 0.64 +0.9 556,341 + BBR_RS12495 0.71 -0.6 556,355 - BBR_RS12495 0.72 +0.0 556,360 + BBR_RS12495 0.73 +0.1 556,377 + BBR_RS12495 0.74 -1.7 556,377 + BBR_RS12495 0.74 +0.6 556,377 + BBR_RS12495 0.74 -0.7 556,377 + BBR_RS12495 0.74 +0.1 556,377 + BBR_RS12495 0.74 +1.3 556,385 - BBR_RS12495 0.75 +0.3 556,389 + BBR_RS12495 0.75 +1.5 556,389 + BBR_RS12495 0.75 -1.1 556,397 - BBR_RS12495 0.75 +0.2 556,399 + BBR_RS12495 0.76 +0.2 556,399 + BBR_RS12495 0.76 -1.4 556,412 - BBR_RS12495 0.77 -0.1 556,416 + BBR_RS12495 0.77 -0.5 556,426 - BBR_RS12495 0.78 +0.3 556,454 - BBR_RS12495 0.80 +1.5 556,503 - BBR_RS12495 0.83 +0.3 556,558 + BBR_RS12495 0.87 +0.2 556,586 - BBR_RS12495 0.89 +0.5 556,594 + -1.1 556,595 - +0.7 556,610 + -1.2 556,610 + +0.4 556,620 + -1.3 556,626 + -2.6 556,626 + -0.3 556,626 - -2.0 556,634 - +0.0 556,634 - -0.9 556,634 - +0.3 556,636 + -0.2 556,644 - -1.8 556,654 - -1.0 556,658 - -0.4 556,660 + +0.7 556,660 + +0.7 556,660 + +0.2 556,660 + +0.1 556,660 + -0.2 556,660 + +0.2 556,660 + +0.2 556,660 + -0.3 556,668 - +0.5 556,668 - +0.5 556,668 - +1.2 556,668 - -0.5 556,668 - -0.3 556,706 + -1.2 556,706 - +0.4 556,712 - -0.2 556,712 - +0.2 556,712 - -0.5 556,714 - -2.4 556,757 - +0.4 556,758 + -0.0 556,758 + +0.6 556,761 - +0.5 556,766 - -0.1 556,771 + -0.2 556,805 - -0.5 556,805 - -1.2 556,818 + -0.1 556,824 - -0.3 556,826 - +0.0 556,842 - -0.1 556,850 - +0.3 556,855 + -0.3 556,860 + -0.7 556,863 - +0.1 556,863 - -0.7 556,863 - -0.1 556,865 + -0.2 556,873 - -1.0 556,878 - -0.0 556,897 + +1.1 556,903 - +0.2 556,905 - +1.2 556,909 - +0.2 556,922 - +0.6 556,997 - +0.8 557,012 + -1.8 557,012 + -1.4 557,012 + +1.4 557,024 + +0.5 557,024 + -0.6 557,024 + -0.5 557,024 + -0.7 557,024 + -0.5 557,024 + -0.1 557,024 + +0.1 557,024 + +0.1 557,024 + -0.2 557,024 + -0.4 557,025 - -0.1 557,032 - +0.1 557,052 - +0.2 557,052 - +0.8 557,081 - BBR_RS12500 0.12 -0.5 558,799 + BBR_RS12505 0.84 +0.1 558,823 + BBR_RS12505 0.86 -1.4
Or see this region's nucleotide sequence