Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12455 and BBR_RS12460 are separated by 161 nucleotides BBR_RS12460 and BBR_RS20700 are separated by 163 nucleotides BBR_RS20700 and BBR_RS12470 are separated by 281 nucleotides
BBR_RS12455: BBR_RS12455 - ribonuclease HII, at 543,739 to 544,596
_RS12455
BBR_RS12460: BBR_RS12460 - LacI family DNA-binding transcriptional regulator, at 544,758 to 545,873
_RS12460
BBR_RS20700: BBR_RS20700 - ribulose phosphate epimerase, at 546,037 to 546,156
_RS20700
BBR_RS12470: BBR_RS12470 - long-chain fatty acid--CoA ligase, at 546,438 to 548,261
_RS12470
Position (kb) 544
545
546 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 543.793 kb on - strand at 543.834 kb on - strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.982 kb on - strand, within BBR_RS12455 at 543.996 kb on + strand, within BBR_RS12455 at 543.996 kb on + strand, within BBR_RS12455 at 543.996 kb on + strand, within BBR_RS12455 at 544.065 kb on + strand, within BBR_RS12455 at 544.065 kb on + strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.082 kb on - strand, within BBR_RS12455 at 544.082 kb on - strand, within BBR_RS12455 at 544.082 kb on - strand, within BBR_RS12455 at 544.084 kb on + strand, within BBR_RS12455 at 544.084 kb on + strand, within BBR_RS12455 at 544.092 kb on - strand, within BBR_RS12455 at 544.092 kb on - strand, within BBR_RS12455 at 544.101 kb on + strand, within BBR_RS12455 at 544.101 kb on + strand, within BBR_RS12455 at 544.109 kb on - strand, within BBR_RS12455 at 544.136 kb on + strand, within BBR_RS12455 at 544.138 kb on - strand, within BBR_RS12455 at 544.150 kb on - strand, within BBR_RS12455 at 544.154 kb on - strand, within BBR_RS12455 at 544.164 kb on - strand, within BBR_RS12455 at 544.170 kb on + strand, within BBR_RS12455 at 544.176 kb on + strand, within BBR_RS12455 at 544.178 kb on - strand, within BBR_RS12455 at 544.181 kb on + strand, within BBR_RS12455 at 544.181 kb on + strand, within BBR_RS12455 at 544.187 kb on + strand, within BBR_RS12455 at 544.224 kb on - strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.254 kb on - strand, within BBR_RS12455 at 544.256 kb on - strand, within BBR_RS12455 at 544.256 kb on - strand, within BBR_RS12455 at 544.266 kb on + strand, within BBR_RS12455 at 544.272 kb on + strand, within BBR_RS12455 at 544.301 kb on + strand, within BBR_RS12455 at 544.324 kb on + strand, within BBR_RS12455 at 544.326 kb on - strand, within BBR_RS12455 at 544.334 kb on + strand, within BBR_RS12455 at 544.337 kb on - strand, within BBR_RS12455 at 544.380 kb on + strand, within BBR_RS12455 at 544.380 kb on + strand, within BBR_RS12455 at 544.380 kb on + strand, within BBR_RS12455 at 544.381 kb on + strand, within BBR_RS12455 at 544.388 kb on - strand, within BBR_RS12455 at 544.395 kb on + strand, within BBR_RS12455 at 544.398 kb on - strand, within BBR_RS12455 at 544.403 kb on - strand, within BBR_RS12455 at 544.415 kb on + strand, within BBR_RS12455 at 544.425 kb on - strand, within BBR_RS12455 at 544.448 kb on + strand, within BBR_RS12455 at 544.460 kb on + strand, within BBR_RS12455 at 544.460 kb on + strand, within BBR_RS12455 at 544.466 kb on - strand, within BBR_RS12455 at 544.499 kb on - strand, within BBR_RS12455 at 544.503 kb on - strand, within BBR_RS12455 at 544.506 kb on + strand, within BBR_RS12455 at 544.512 kb on + strand at 544.521 kb on - strand at 544.521 kb on - strand at 544.554 kb on - strand at 544.564 kb on + strand at 544.570 kb on - strand at 544.570 kb on - strand at 544.598 kb on - strand at 544.608 kb on + strand at 544.608 kb on + strand at 544.611 kb on - strand at 544.624 kb on + strand at 544.713 kb on - strand at 544.725 kb on + strand at 544.739 kb on + strand at 544.746 kb on + strand at 544.805 kb on + strand at 544.833 kb on + strand at 544.833 kb on + strand at 544.841 kb on - strand at 544.866 kb on + strand at 544.866 kb on - strand at 544.868 kb on + strand at 544.868 kb on + strand at 544.895 kb on - strand, within BBR_RS12460 at 544.900 kb on + strand, within BBR_RS12460 at 544.918 kb on - strand, within BBR_RS12460 at 544.918 kb on - strand, within BBR_RS12460 at 544.933 kb on - strand, within BBR_RS12460 at 544.955 kb on - strand, within BBR_RS12460 at 544.995 kb on - strand, within BBR_RS12460 at 545.044 kb on + strand, within BBR_RS12460 at 545.044 kb on + strand, within BBR_RS12460 at 545.052 kb on - strand, within BBR_RS12460 at 545.054 kb on + strand, within BBR_RS12460 at 545.083 kb on - strand, within BBR_RS12460 at 545.096 kb on + strand, within BBR_RS12460 at 545.096 kb on + strand, within BBR_RS12460 at 545.122 kb on + strand, within BBR_RS12460 at 545.132 kb on + strand, within BBR_RS12460 at 545.137 kb on + strand, within BBR_RS12460 at 545.138 kb on - strand, within BBR_RS12460 at 545.177 kb on - strand, within BBR_RS12460 at 545.177 kb on - strand, within BBR_RS12460 at 545.186 kb on + strand, within BBR_RS12460 at 545.192 kb on - strand, within BBR_RS12460 at 545.268 kb on - strand, within BBR_RS12460 at 545.291 kb on + strand, within BBR_RS12460 at 545.303 kb on + strand, within BBR_RS12460 at 545.313 kb on + strand, within BBR_RS12460 at 545.318 kb on + strand, within BBR_RS12460 at 545.320 kb on + strand, within BBR_RS12460 at 545.338 kb on - strand, within BBR_RS12460 at 545.345 kb on - strand, within BBR_RS12460 at 545.371 kb on - strand, within BBR_RS12460 at 545.371 kb on - strand, within BBR_RS12460 at 545.375 kb on + strand, within BBR_RS12460 at 545.378 kb on + strand, within BBR_RS12460 at 545.419 kb on + strand, within BBR_RS12460 at 545.420 kb on + strand, within BBR_RS12460 at 545.469 kb on + strand, within BBR_RS12460 at 545.477 kb on + strand, within BBR_RS12460 at 545.479 kb on + strand, within BBR_RS12460 at 545.483 kb on + strand, within BBR_RS12460 at 545.547 kb on + strand, within BBR_RS12460 at 545.563 kb on + strand, within BBR_RS12460 at 545.563 kb on + strand, within BBR_RS12460 at 545.624 kb on - strand, within BBR_RS12460 at 545.682 kb on + strand, within BBR_RS12460 at 545.686 kb on + strand, within BBR_RS12460 at 545.687 kb on + strand, within BBR_RS12460 at 545.695 kb on - strand, within BBR_RS12460 at 545.707 kb on - strand, within BBR_RS12460 at 545.742 kb on - strand, within BBR_RS12460 at 545.763 kb on - strand at 545.775 kb on - strand at 545.798 kb on - strand at 545.814 kb on + strand at 545.839 kb on - strand at 545.848 kb on + strand at 545.854 kb on + strand at 545.857 kb on - strand at 545.929 kb on + strand at 545.929 kb on + strand at 545.934 kb on + strand at 545.934 kb on + strand at 545.934 kb on + strand at 545.937 kb on + strand at 545.937 kb on - strand at 545.981 kb on + strand at 545.981 kb on + strand at 545.999 kb on - strand at 546.004 kb on - strand at 546.123 kb on - strand, within BBR_RS20700 at 546.131 kb on - strand, within BBR_RS20700 at 546.131 kb on - strand, within BBR_RS20700 at 546.134 kb on + strand, within BBR_RS20700 at 546.146 kb on + strand at 546.146 kb on + strand at 546.166 kb on + strand at 546.166 kb on + strand at 546.181 kb on + strand at 546.186 kb on - strand at 546.222 kb on + strand at 546.232 kb on + strand at 546.232 kb on + strand at 546.247 kb on + strand at 546.249 kb on - strand at 546.266 kb on + strand at 546.274 kb on - strand at 546.284 kb on + strand at 546.340 kb on + strand at 546.348 kb on + strand at 546.348 kb on + strand at 546.356 kb on - strand at 546.378 kb on - strand at 546.381 kb on - strand at 546.421 kb on + strand at 546.421 kb on + strand at 546.421 kb on + strand at 546.473 kb on + strand at 546.497 kb on - strand at 546.497 kb on - strand at 546.497 kb on - strand at 546.532 kb on + strand at 546.535 kb on - strand at 546.549 kb on - strand at 546.549 kb on - strand at 546.614 kb on - strand at 546.621 kb on - strand, within BBR_RS12470 at 546.646 kb on + strand, within BBR_RS12470 at 546.661 kb on + strand, within BBR_RS12470 at 546.671 kb on - strand, within BBR_RS12470 at 546.684 kb on - strand, within BBR_RS12470 at 546.694 kb on - strand, within BBR_RS12470 at 546.698 kb on - strand, within BBR_RS12470 at 546.709 kb on - strand, within BBR_RS12470 at 546.716 kb on + strand, within BBR_RS12470 at 546.727 kb on + strand, within BBR_RS12470 at 546.727 kb on + strand, within BBR_RS12470 at 546.727 kb on + strand, within BBR_RS12470 at 546.735 kb on - strand, within BBR_RS12470 at 546.735 kb on - strand, within BBR_RS12470 at 546.756 kb on - strand, within BBR_RS12470 at 546.767 kb on + strand, within BBR_RS12470 at 546.767 kb on - strand, within BBR_RS12470 at 546.767 kb on - strand, within BBR_RS12470 at 546.775 kb on - strand, within BBR_RS12470 at 546.787 kb on + strand, within BBR_RS12470 at 546.791 kb on + strand, within BBR_RS12470 at 546.795 kb on - strand, within BBR_RS12470 at 546.812 kb on - strand, within BBR_RS12470 at 546.817 kb on - strand, within BBR_RS12470 at 546.818 kb on + strand, within BBR_RS12470
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 543,793 - +0.7 543,834 - BBR_RS12455 0.11 +0.3 543,964 + BBR_RS12455 0.26 -0.2 543,964 + BBR_RS12455 0.26 -0.1 543,964 + BBR_RS12455 0.26 -0.1 543,964 + BBR_RS12455 0.26 -0.0 543,964 + BBR_RS12455 0.26 -1.1 543,982 - BBR_RS12455 0.28 -0.0 543,996 + BBR_RS12455 0.30 +0.7 543,996 + BBR_RS12455 0.30 -1.0 543,996 + BBR_RS12455 0.30 +0.4 544,065 + BBR_RS12455 0.38 +0.9 544,065 + BBR_RS12455 0.38 -0.0 544,073 - BBR_RS12455 0.39 +0.4 544,073 - BBR_RS12455 0.39 -0.1 544,073 - BBR_RS12455 0.39 +0.8 544,073 - BBR_RS12455 0.39 -0.7 544,082 - BBR_RS12455 0.40 +1.1 544,082 - BBR_RS12455 0.40 +0.5 544,082 - BBR_RS12455 0.40 +0.2 544,084 + BBR_RS12455 0.40 +0.0 544,084 + BBR_RS12455 0.40 +0.7 544,092 - BBR_RS12455 0.41 -0.4 544,092 - BBR_RS12455 0.41 -0.7 544,101 + BBR_RS12455 0.42 +0.1 544,101 + BBR_RS12455 0.42 -0.1 544,109 - BBR_RS12455 0.43 +0.6 544,136 + BBR_RS12455 0.46 -0.5 544,138 - BBR_RS12455 0.47 -0.1 544,150 - BBR_RS12455 0.48 +0.7 544,154 - BBR_RS12455 0.48 -0.7 544,164 - BBR_RS12455 0.50 +0.6 544,170 + BBR_RS12455 0.50 -0.5 544,176 + BBR_RS12455 0.51 -0.4 544,178 - BBR_RS12455 0.51 -0.6 544,181 + BBR_RS12455 0.52 +0.5 544,181 + BBR_RS12455 0.52 -0.1 544,187 + BBR_RS12455 0.52 -0.7 544,224 - BBR_RS12455 0.57 -0.3 544,232 + BBR_RS12455 0.57 -0.5 544,232 + BBR_RS12455 0.57 -0.0 544,232 + BBR_RS12455 0.57 -0.7 544,232 + BBR_RS12455 0.57 -0.4 544,232 + BBR_RS12455 0.57 -0.2 544,232 + BBR_RS12455 0.57 -0.3 544,254 - BBR_RS12455 0.60 -0.4 544,256 - BBR_RS12455 0.60 +0.7 544,256 - BBR_RS12455 0.60 -0.2 544,266 + BBR_RS12455 0.61 -0.3 544,272 + BBR_RS12455 0.62 -0.4 544,301 + BBR_RS12455 0.66 -0.7 544,324 + BBR_RS12455 0.68 -0.3 544,326 - BBR_RS12455 0.68 -0.6 544,334 + BBR_RS12455 0.69 +1.7 544,337 - BBR_RS12455 0.70 +1.3 544,380 + BBR_RS12455 0.75 -0.9 544,380 + BBR_RS12455 0.75 -0.2 544,380 + BBR_RS12455 0.75 +0.3 544,381 + BBR_RS12455 0.75 +0.1 544,388 - BBR_RS12455 0.76 -0.2 544,395 + BBR_RS12455 0.76 -0.6 544,398 - BBR_RS12455 0.77 -0.4 544,403 - BBR_RS12455 0.77 +0.4 544,415 + BBR_RS12455 0.79 -0.5 544,425 - BBR_RS12455 0.80 +1.8 544,448 + BBR_RS12455 0.83 -0.0 544,460 + BBR_RS12455 0.84 -1.2 544,460 + BBR_RS12455 0.84 -0.4 544,466 - BBR_RS12455 0.85 +1.6 544,499 - BBR_RS12455 0.89 -0.3 544,503 - BBR_RS12455 0.89 -0.9 544,506 + BBR_RS12455 0.89 +0.7 544,512 + +0.4 544,521 - +0.5 544,521 - -0.7 544,554 - -1.1 544,564 + +0.2 544,570 - +1.1 544,570 - -1.1 544,598 - +0.1 544,608 + -1.0 544,608 + +0.7 544,611 - -0.3 544,624 + -0.1 544,713 - +0.8 544,725 + +0.4 544,739 + -0.0 544,746 + -0.7 544,805 + -0.5 544,833 + -1.8 544,833 + -0.7 544,841 - -1.7 544,866 + +0.4 544,866 - -2.2 544,868 + -1.0 544,868 + -0.6 544,895 - BBR_RS12460 0.12 -1.9 544,900 + BBR_RS12460 0.13 -1.6 544,918 - BBR_RS12460 0.14 -0.9 544,918 - BBR_RS12460 0.14 +0.6 544,933 - BBR_RS12460 0.16 -1.7 544,955 - BBR_RS12460 0.18 +0.2 544,995 - BBR_RS12460 0.21 -0.8 545,044 + BBR_RS12460 0.26 -1.1 545,044 + BBR_RS12460 0.26 -1.6 545,052 - BBR_RS12460 0.26 -1.3 545,054 + BBR_RS12460 0.27 +0.1 545,083 - BBR_RS12460 0.29 +0.6 545,096 + BBR_RS12460 0.30 -1.3 545,096 + BBR_RS12460 0.30 -1.9 545,122 + BBR_RS12460 0.33 -1.6 545,132 + BBR_RS12460 0.34 -0.0 545,137 + BBR_RS12460 0.34 -1.6 545,138 - BBR_RS12460 0.34 +1.6 545,177 - BBR_RS12460 0.38 -0.0 545,177 - BBR_RS12460 0.38 -0.0 545,186 + BBR_RS12460 0.38 +1.4 545,192 - BBR_RS12460 0.39 -1.3 545,268 - BBR_RS12460 0.46 -0.8 545,291 + BBR_RS12460 0.48 -2.4 545,303 + BBR_RS12460 0.49 -1.2 545,313 + BBR_RS12460 0.50 -0.9 545,318 + BBR_RS12460 0.50 -1.3 545,320 + BBR_RS12460 0.50 -0.8 545,338 - BBR_RS12460 0.52 -0.4 545,345 - BBR_RS12460 0.53 +0.4 545,371 - BBR_RS12460 0.55 -0.5 545,371 - BBR_RS12460 0.55 +0.2 545,375 + BBR_RS12460 0.55 -1.9 545,378 + BBR_RS12460 0.56 -1.2 545,419 + BBR_RS12460 0.59 -0.9 545,420 + BBR_RS12460 0.59 -0.4 545,469 + BBR_RS12460 0.64 -0.7 545,477 + BBR_RS12460 0.64 -0.6 545,479 + BBR_RS12460 0.65 -0.5 545,483 + BBR_RS12460 0.65 -1.7 545,547 + BBR_RS12460 0.71 +1.4 545,563 + BBR_RS12460 0.72 -0.2 545,563 + BBR_RS12460 0.72 -0.3 545,624 - BBR_RS12460 0.78 -2.9 545,682 + BBR_RS12460 0.83 +0.5 545,686 + BBR_RS12460 0.83 +2.0 545,687 + BBR_RS12460 0.83 -1.0 545,695 - BBR_RS12460 0.84 -1.7 545,707 - BBR_RS12460 0.85 -1.1 545,742 - BBR_RS12460 0.88 -2.5 545,763 - +0.4 545,775 - +0.9 545,798 - +1.0 545,814 + -0.4 545,839 - +2.0 545,848 + +1.4 545,854 + -0.1 545,857 - -2.1 545,929 + +0.6 545,929 + +1.0 545,934 + -1.1 545,934 + +0.0 545,934 + -0.0 545,937 + -0.5 545,937 - +0.9 545,981 + -0.3 545,981 + +0.2 545,999 - -0.1 546,004 - +0.1 546,123 - BBR_RS20700 0.72 -0.4 546,131 - BBR_RS20700 0.78 -0.3 546,131 - BBR_RS20700 0.78 +0.3 546,134 + BBR_RS20700 0.81 +0.4 546,146 + +0.7 546,146 + -0.3 546,166 + -0.6 546,166 + -1.5 546,181 + -0.2 546,186 - -0.1 546,222 + -0.4 546,232 + +0.1 546,232 + -0.1 546,247 + -0.1 546,249 - +0.9 546,266 + -0.4 546,274 - +0.2 546,284 + -0.3 546,340 + +0.4 546,348 + +0.0 546,348 + -0.9 546,356 - -0.5 546,378 - +0.3 546,381 - +0.8 546,421 + -0.3 546,421 + +0.7 546,421 + +0.9 546,473 + -1.7 546,497 - -1.2 546,497 - -0.2 546,497 - -1.1 546,532 + +1.4 546,535 - -1.7 546,549 - +0.1 546,549 - -0.6 546,614 - +0.5 546,621 - BBR_RS12470 0.10 -0.9 546,646 + BBR_RS12470 0.11 -0.1 546,661 + BBR_RS12470 0.12 -0.5 546,671 - BBR_RS12470 0.13 -0.6 546,684 - BBR_RS12470 0.13 -1.6 546,694 - BBR_RS12470 0.14 -1.6 546,698 - BBR_RS12470 0.14 -0.5 546,709 - BBR_RS12470 0.15 -0.4 546,716 + BBR_RS12470 0.15 -2.3 546,727 + BBR_RS12470 0.16 +0.6 546,727 + BBR_RS12470 0.16 -0.4 546,727 + BBR_RS12470 0.16 -1.2 546,735 - BBR_RS12470 0.16 -0.5 546,735 - BBR_RS12470 0.16 -0.3 546,756 - BBR_RS12470 0.17 -0.3 546,767 + BBR_RS12470 0.18 -0.1 546,767 - BBR_RS12470 0.18 +0.5 546,767 - BBR_RS12470 0.18 -0.3 546,775 - BBR_RS12470 0.18 -1.4 546,787 + BBR_RS12470 0.19 -1.1 546,791 + BBR_RS12470 0.19 +0.2 546,795 - BBR_RS12470 0.20 +1.6 546,812 - BBR_RS12470 0.21 -0.1 546,817 - BBR_RS12470 0.21 -0.9 546,818 + BBR_RS12470 0.21 +0.2
Or see this region's nucleotide sequence