Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12305 and BBR_RS12310 are separated by 251 nucleotides BBR_RS12310 and BBR_RS20690 are separated by 38 nucleotides BBR_RS20690 and BBR_RS12315 are separated by 115 nucleotides
BBR_RS12305: BBR_RS12305 - endonuclease/exonuclease/phosphatase family protein, at 510,658 to 511,989
_RS12305
BBR_RS12310: BBR_RS12310 - signal recognition particle protein, at 512,241 to 513,896
_RS12310
BBR_RS20690: BBR_RS20690 - hypothetical protein, at 513,935 to 514,135
_RS20690
BBR_RS12315: BBR_RS12315 - cation transporter, at 514,251 to 515,159
_RS12315
Position (kb) 512
513
514 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 511.242 kb on - strand, within BBR_RS12305 at 511.254 kb on - strand, within BBR_RS12305 at 511.300 kb on + strand, within BBR_RS12305 at 511.410 kb on + strand, within BBR_RS12305 at 511.436 kb on + strand, within BBR_RS12305 at 511.507 kb on - strand, within BBR_RS12305 at 511.547 kb on + strand, within BBR_RS12305 at 511.571 kb on - strand, within BBR_RS12305 at 511.573 kb on - strand, within BBR_RS12305 at 511.622 kb on + strand, within BBR_RS12305 at 511.622 kb on + strand, within BBR_RS12305 at 511.635 kb on - strand, within BBR_RS12305 at 511.644 kb on - strand, within BBR_RS12305 at 511.701 kb on + strand, within BBR_RS12305 at 511.701 kb on + strand, within BBR_RS12305 at 511.718 kb on + strand, within BBR_RS12305 at 511.744 kb on - strand, within BBR_RS12305 at 511.751 kb on + strand, within BBR_RS12305 at 511.758 kb on + strand, within BBR_RS12305 at 511.775 kb on + strand, within BBR_RS12305 at 511.777 kb on + strand, within BBR_RS12305 at 511.793 kb on + strand, within BBR_RS12305 at 511.852 kb on + strand, within BBR_RS12305 at 511.860 kb on - strand at 511.865 kb on + strand at 511.875 kb on - strand at 511.886 kb on + strand at 511.925 kb on - strand at 511.941 kb on - strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.943 kb on + strand at 511.951 kb on - strand at 511.951 kb on - strand at 511.965 kb on + strand at 511.970 kb on + strand at 511.970 kb on + strand at 511.978 kb on - strand at 512.039 kb on - strand at 512.051 kb on + strand at 512.057 kb on - strand at 512.062 kb on - strand at 512.062 kb on - strand at 512.063 kb on - strand at 512.087 kb on - strand at 512.112 kb on - strand at 512.159 kb on - strand at 512.188 kb on - strand at 512.235 kb on + strand at 512.258 kb on - strand at 512.285 kb on - strand at 512.288 kb on + strand at 512.296 kb on - strand at 512.321 kb on + strand at 512.329 kb on - strand at 512.329 kb on - strand at 512.329 kb on - strand at 512.341 kb on + strand at 512.422 kb on + strand, within BBR_RS12310 at 512.435 kb on - strand, within BBR_RS12310 at 512.559 kb on - strand, within BBR_RS12310 at 513.898 kb on - strand at 513.979 kb on - strand, within BBR_RS20690 at 513.979 kb on - strand, within BBR_RS20690 at 514.043 kb on + strand, within BBR_RS20690 at 514.090 kb on - strand, within BBR_RS20690 at 514.152 kb on + strand at 514.166 kb on + strand at 514.166 kb on + strand at 514.200 kb on + strand at 514.281 kb on + strand at 514.289 kb on - strand at 514.319 kb on - strand at 514.359 kb on + strand, within BBR_RS12315 at 514.437 kb on + strand, within BBR_RS12315 at 514.439 kb on + strand, within BBR_RS12315 at 514.439 kb on + strand, within BBR_RS12315 at 514.441 kb on + strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.452 kb on - strand, within BBR_RS12315 at 514.469 kb on + strand, within BBR_RS12315 at 514.482 kb on - strand, within BBR_RS12315 at 514.482 kb on - strand, within BBR_RS12315 at 514.496 kb on + strand, within BBR_RS12315 at 514.511 kb on - strand, within BBR_RS12315 at 514.534 kb on - strand, within BBR_RS12315 at 514.550 kb on - strand, within BBR_RS12315 at 514.557 kb on + strand, within BBR_RS12315 at 514.572 kb on + strand, within BBR_RS12315 at 514.592 kb on + strand, within BBR_RS12315 at 514.592 kb on + strand, within BBR_RS12315 at 514.600 kb on - strand, within BBR_RS12315 at 514.631 kb on - strand, within BBR_RS12315 at 514.642 kb on + strand, within BBR_RS12315 at 514.648 kb on + strand, within BBR_RS12315 at 514.650 kb on - strand, within BBR_RS12315 at 514.650 kb on - strand, within BBR_RS12315 at 514.650 kb on - strand, within BBR_RS12315 at 514.656 kb on - strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.699 kb on - strand, within BBR_RS12315 at 514.711 kb on - strand, within BBR_RS12315 at 514.758 kb on + strand, within BBR_RS12315 at 514.761 kb on - strand, within BBR_RS12315 at 514.768 kb on + strand, within BBR_RS12315 at 514.800 kb on + strand, within BBR_RS12315 at 514.822 kb on - strand, within BBR_RS12315 at 514.822 kb on - strand, within BBR_RS12315 at 514.827 kb on - strand, within BBR_RS12315 at 514.827 kb on - strand, within BBR_RS12315 at 514.847 kb on - strand, within BBR_RS12315 at 514.852 kb on - strand, within BBR_RS12315 at 514.864 kb on + strand, within BBR_RS12315 at 514.864 kb on + strand, within BBR_RS12315 at 514.872 kb on - strand, within BBR_RS12315
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 511,242 - BBR_RS12305 0.44 -0.7 511,254 - BBR_RS12305 0.45 -0.7 511,300 + BBR_RS12305 0.48 -1.2 511,410 + BBR_RS12305 0.56 -1.0 511,436 + BBR_RS12305 0.58 +0.0 511,507 - BBR_RS12305 0.64 +0.9 511,547 + BBR_RS12305 0.67 -1.3 511,571 - BBR_RS12305 0.69 -1.3 511,573 - BBR_RS12305 0.69 +0.1 511,622 + BBR_RS12305 0.72 -1.0 511,622 + BBR_RS12305 0.72 +0.8 511,635 - BBR_RS12305 0.73 +1.4 511,644 - BBR_RS12305 0.74 +0.6 511,701 + BBR_RS12305 0.78 -1.2 511,701 + BBR_RS12305 0.78 -2.1 511,718 + BBR_RS12305 0.80 -1.9 511,744 - BBR_RS12305 0.82 +0.3 511,751 + BBR_RS12305 0.82 -0.8 511,758 + BBR_RS12305 0.83 -1.8 511,775 + BBR_RS12305 0.84 +0.4 511,777 + BBR_RS12305 0.84 -2.5 511,793 + BBR_RS12305 0.85 -1.7 511,852 + BBR_RS12305 0.90 -0.3 511,860 - -0.3 511,865 + +0.5 511,875 - -0.6 511,886 + -2.4 511,925 - -1.9 511,941 - -0.4 511,943 + +0.5 511,943 + +0.7 511,943 + -2.5 511,943 + -2.7 511,943 + +0.3 511,943 + -0.1 511,943 + -0.9 511,943 + -1.6 511,951 - -2.5 511,951 - +1.0 511,965 + +0.2 511,970 + -0.1 511,970 + +0.4 511,978 - -0.2 512,039 - -0.8 512,051 + +0.3 512,057 - -0.3 512,062 - +0.3 512,062 - +0.2 512,063 - +0.2 512,087 - -0.7 512,112 - -0.2 512,159 - -0.4 512,188 - +0.4 512,235 + -0.7 512,258 - +0.3 512,285 - -0.3 512,288 + +0.7 512,296 - -0.5 512,321 + +0.7 512,329 - -0.5 512,329 - +0.1 512,329 - +0.0 512,341 + -0.5 512,422 + BBR_RS12310 0.11 -0.9 512,435 - BBR_RS12310 0.12 -0.6 512,559 - BBR_RS12310 0.19 -0.6 513,898 - +1.1 513,979 - BBR_RS20690 0.22 -2.4 513,979 - BBR_RS20690 0.22 -1.9 514,043 + BBR_RS20690 0.54 +1.2 514,090 - BBR_RS20690 0.77 +0.1 514,152 + -0.5 514,166 + -0.5 514,166 + -0.9 514,200 + -0.6 514,281 + -0.3 514,289 - +0.0 514,319 - +0.4 514,359 + BBR_RS12315 0.12 +2.5 514,437 + BBR_RS12315 0.20 +0.4 514,439 + BBR_RS12315 0.21 -0.0 514,439 + BBR_RS12315 0.21 -0.3 514,441 + BBR_RS12315 0.21 +0.9 514,445 - BBR_RS12315 0.21 +1.2 514,445 - BBR_RS12315 0.21 +0.6 514,445 - BBR_RS12315 0.21 +0.5 514,445 - BBR_RS12315 0.21 +0.9 514,445 - BBR_RS12315 0.21 -0.7 514,452 - BBR_RS12315 0.22 -0.1 514,469 + BBR_RS12315 0.24 +0.7 514,482 - BBR_RS12315 0.25 -0.2 514,482 - BBR_RS12315 0.25 -0.4 514,496 + BBR_RS12315 0.27 +0.6 514,511 - BBR_RS12315 0.29 +0.3 514,534 - BBR_RS12315 0.31 +2.7 514,550 - BBR_RS12315 0.33 +0.6 514,557 + BBR_RS12315 0.34 -0.1 514,572 + BBR_RS12315 0.35 +0.2 514,592 + BBR_RS12315 0.38 +0.7 514,592 + BBR_RS12315 0.38 +0.9 514,600 - BBR_RS12315 0.38 -0.2 514,631 - BBR_RS12315 0.42 +0.3 514,642 + BBR_RS12315 0.43 +0.2 514,648 + BBR_RS12315 0.44 +0.7 514,650 - BBR_RS12315 0.44 +1.6 514,650 - BBR_RS12315 0.44 +0.6 514,650 - BBR_RS12315 0.44 +0.5 514,656 - BBR_RS12315 0.45 +0.0 514,691 + BBR_RS12315 0.48 -2.5 514,691 + BBR_RS12315 0.48 +0.2 514,691 + BBR_RS12315 0.48 -0.0 514,691 + BBR_RS12315 0.48 +0.7 514,691 + BBR_RS12315 0.48 +0.7 514,691 + BBR_RS12315 0.48 +0.6 514,699 - BBR_RS12315 0.49 +0.1 514,711 - BBR_RS12315 0.51 +0.8 514,758 + BBR_RS12315 0.56 -1.5 514,761 - BBR_RS12315 0.56 +0.3 514,768 + BBR_RS12315 0.57 +0.9 514,800 + BBR_RS12315 0.60 +1.0 514,822 - BBR_RS12315 0.63 +3.3 514,822 - BBR_RS12315 0.63 +0.5 514,827 - BBR_RS12315 0.63 +2.0 514,827 - BBR_RS12315 0.63 +0.6 514,847 - BBR_RS12315 0.66 +1.3 514,852 - BBR_RS12315 0.66 +0.3 514,864 + BBR_RS12315 0.67 -0.7 514,864 + BBR_RS12315 0.67 +0.5 514,872 - BBR_RS12315 0.68 -0.1
Or see this region's nucleotide sequence