Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12165 and BBR_RS12170 are separated by 254 nucleotides BBR_RS12170 and BBR_RS12175 are separated by 27 nucleotides BBR_RS12175 and BBR_RS12180 are separated by 71 nucleotides
BBR_RS12165: BBR_RS12165 - ATP-binding protein, at 478,785 to 480,008
_RS12165
BBR_RS12170: BBR_RS12170 - IS256 family transposase, at 480,263 to 480,628
_RS12170
BBR_RS12175: BBR_RS12175 - TetR/AcrR family transcriptional regulator, at 480,656 to 481,321
_RS12175
BBR_RS12180: BBR_RS12180 - DUF2974 domain-containing protein, at 481,393 to 482,730
_RS12180
Position (kb) 480
481
482 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 479.700 kb on - strand, within BBR_RS12165 at 479.707 kb on + strand, within BBR_RS12165 at 479.742 kb on - strand, within BBR_RS12165 at 479.754 kb on + strand, within BBR_RS12165 at 479.767 kb on + strand, within BBR_RS12165 at 479.780 kb on - strand, within BBR_RS12165 at 479.794 kb on + strand, within BBR_RS12165 at 479.838 kb on + strand, within BBR_RS12165 at 479.890 kb on + strand at 479.924 kb on - strand at 479.926 kb on + strand at 479.936 kb on - strand at 479.943 kb on - strand at 479.950 kb on - strand at 479.960 kb on - strand at 479.960 kb on - strand at 480.113 kb on + strand at 480.155 kb on - strand at 480.159 kb on + strand at 480.159 kb on - strand at 480.159 kb on - strand at 480.162 kb on - strand at 480.162 kb on - strand at 480.167 kb on - strand at 480.173 kb on + strand at 480.194 kb on - strand at 480.261 kb on + strand at 480.274 kb on - strand at 480.281 kb on + strand at 480.281 kb on + strand at 480.289 kb on - strand at 480.292 kb on + strand at 480.292 kb on + strand at 480.299 kb on - strand at 480.301 kb on + strand, within BBR_RS12170 at 480.319 kb on - strand, within BBR_RS12170 at 480.357 kb on + strand, within BBR_RS12170 at 480.357 kb on - strand, within BBR_RS12170 at 480.359 kb on - strand, within BBR_RS12170 at 480.359 kb on - strand, within BBR_RS12170 at 480.398 kb on - strand, within BBR_RS12170 at 480.470 kb on - strand, within BBR_RS12170 at 480.483 kb on - strand, within BBR_RS12170 at 480.504 kb on - strand, within BBR_RS12170 at 480.504 kb on - strand, within BBR_RS12170 at 480.512 kb on + strand, within BBR_RS12170 at 480.552 kb on - strand, within BBR_RS12170 at 480.554 kb on + strand, within BBR_RS12170 at 480.602 kb on + strand at 480.616 kb on - strand at 480.635 kb on - strand at 480.643 kb on - strand at 480.647 kb on + strand at 480.650 kb on - strand at 480.650 kb on - strand at 480.654 kb on + strand at 480.654 kb on + strand at 480.654 kb on + strand at 480.654 kb on + strand at 480.654 kb on + strand at 480.660 kb on - strand at 480.662 kb on - strand at 480.678 kb on + strand at 480.689 kb on + strand at 480.703 kb on + strand at 480.711 kb on + strand at 480.723 kb on - strand, within BBR_RS12175 at 480.723 kb on - strand, within BBR_RS12175 at 480.732 kb on - strand, within BBR_RS12175 at 480.770 kb on - strand, within BBR_RS12175 at 480.786 kb on + strand, within BBR_RS12175 at 480.789 kb on - strand, within BBR_RS12175 at 480.789 kb on - strand, within BBR_RS12175 at 480.794 kb on - strand, within BBR_RS12175 at 480.794 kb on - strand, within BBR_RS12175 at 480.794 kb on - strand, within BBR_RS12175 at 480.821 kb on - strand, within BBR_RS12175 at 480.823 kb on + strand, within BBR_RS12175 at 480.823 kb on + strand, within BBR_RS12175 at 480.823 kb on + strand, within BBR_RS12175 at 480.824 kb on - strand, within BBR_RS12175 at 480.831 kb on - strand, within BBR_RS12175 at 480.836 kb on - strand, within BBR_RS12175 at 480.885 kb on + strand, within BBR_RS12175 at 480.885 kb on + strand, within BBR_RS12175 at 480.886 kb on - strand, within BBR_RS12175 at 480.920 kb on - strand, within BBR_RS12175 at 480.957 kb on + strand, within BBR_RS12175 at 480.965 kb on - strand, within BBR_RS12175 at 480.965 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.983 kb on - strand, within BBR_RS12175 at 481.024 kb on + strand, within BBR_RS12175 at 481.053 kb on - strand, within BBR_RS12175 at 481.095 kb on - strand, within BBR_RS12175 at 481.095 kb on - strand, within BBR_RS12175 at 481.095 kb on - strand, within BBR_RS12175 at 481.095 kb on - strand, within BBR_RS12175 at 481.113 kb on + strand, within BBR_RS12175 at 481.252 kb on - strand, within BBR_RS12175 at 481.257 kb on - strand at 481.329 kb on + strand at 481.386 kb on - strand at 481.436 kb on + strand at 481.450 kb on - strand at 481.465 kb on + strand at 481.475 kb on + strand at 481.475 kb on + strand at 481.475 kb on + strand at 481.475 kb on + strand at 481.475 kb on + strand at 481.483 kb on - strand at 481.483 kb on - strand at 481.483 kb on - strand at 481.504 kb on - strand at 481.525 kb on - strand at 481.536 kb on - strand, within BBR_RS12180 at 481.536 kb on - strand, within BBR_RS12180 at 481.575 kb on - strand, within BBR_RS12180 at 481.579 kb on - strand, within BBR_RS12180 at 481.579 kb on - strand, within BBR_RS12180 at 481.590 kb on + strand, within BBR_RS12180 at 481.598 kb on - strand, within BBR_RS12180 at 481.598 kb on - strand, within BBR_RS12180 at 481.598 kb on - strand, within BBR_RS12180 at 481.598 kb on - strand, within BBR_RS12180 at 481.598 kb on - strand, within BBR_RS12180 at 481.599 kb on + strand, within BBR_RS12180 at 481.630 kb on + strand, within BBR_RS12180 at 481.646 kb on + strand, within BBR_RS12180 at 481.649 kb on + strand, within BBR_RS12180 at 481.654 kb on - strand, within BBR_RS12180 at 481.654 kb on - strand, within BBR_RS12180 at 481.663 kb on + strand, within BBR_RS12180 at 481.663 kb on + strand, within BBR_RS12180 at 481.671 kb on + strand, within BBR_RS12180 at 481.671 kb on - strand, within BBR_RS12180 at 481.672 kb on + strand, within BBR_RS12180 at 481.672 kb on + strand, within BBR_RS12180 at 481.672 kb on - strand, within BBR_RS12180 at 481.685 kb on + strand, within BBR_RS12180 at 481.685 kb on + strand, within BBR_RS12180 at 481.685 kb on - strand, within BBR_RS12180 at 481.693 kb on + strand, within BBR_RS12180 at 481.693 kb on - strand, within BBR_RS12180 at 481.695 kb on + strand, within BBR_RS12180 at 481.695 kb on + strand, within BBR_RS12180 at 481.695 kb on + strand, within BBR_RS12180 at 481.703 kb on - strand, within BBR_RS12180 at 481.703 kb on - strand, within BBR_RS12180 at 481.723 kb on + strand, within BBR_RS12180 at 481.741 kb on - strand, within BBR_RS12180 at 481.743 kb on + strand, within BBR_RS12180 at 481.743 kb on + strand, within BBR_RS12180 at 481.743 kb on + strand, within BBR_RS12180 at 481.751 kb on - strand, within BBR_RS12180 at 481.751 kb on - strand, within BBR_RS12180 at 481.751 kb on - strand, within BBR_RS12180 at 481.751 kb on - strand, within BBR_RS12180 at 481.780 kb on - strand, within BBR_RS12180 at 481.780 kb on - strand, within BBR_RS12180 at 481.813 kb on + strand, within BBR_RS12180 at 481.818 kb on + strand, within BBR_RS12180 at 481.818 kb on + strand, within BBR_RS12180 at 481.821 kb on - strand, within BBR_RS12180 at 481.826 kb on - strand, within BBR_RS12180 at 481.826 kb on - strand, within BBR_RS12180 at 481.826 kb on - strand, within BBR_RS12180 at 481.831 kb on - strand, within BBR_RS12180 at 481.841 kb on - strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.922 kb on - strand, within BBR_RS12180 at 481.935 kb on + strand, within BBR_RS12180 at 481.965 kb on + strand, within BBR_RS12180 at 481.973 kb on - strand, within BBR_RS12180 at 481.973 kb on - strand, within BBR_RS12180 at 481.995 kb on - strand, within BBR_RS12180 at 482.010 kb on - strand, within BBR_RS12180 at 482.010 kb on - strand, within BBR_RS12180 at 482.054 kb on + strand, within BBR_RS12180 at 482.062 kb on - strand, within BBR_RS12180 at 482.076 kb on + strand, within BBR_RS12180 at 482.112 kb on + strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.169 kb on + strand, within BBR_RS12180 at 482.170 kb on - strand, within BBR_RS12180 at 482.202 kb on + strand, within BBR_RS12180 at 482.202 kb on + strand, within BBR_RS12180 at 482.214 kb on + strand, within BBR_RS12180 at 482.232 kb on + strand, within BBR_RS12180 at 482.237 kb on + strand, within BBR_RS12180 at 482.240 kb on - strand, within BBR_RS12180 at 482.246 kb on - strand, within BBR_RS12180 at 482.255 kb on - strand, within BBR_RS12180 at 482.303 kb on + strand, within BBR_RS12180 at 482.305 kb on + strand, within BBR_RS12180
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 479,700 - BBR_RS12165 0.75 -0.3 479,707 + BBR_RS12165 0.75 -0.2 479,742 - BBR_RS12165 0.78 -0.3 479,754 + BBR_RS12165 0.79 -1.8 479,767 + BBR_RS12165 0.80 +0.5 479,780 - BBR_RS12165 0.81 +0.7 479,794 + BBR_RS12165 0.82 +0.7 479,838 + BBR_RS12165 0.86 -0.1 479,890 + +0.6 479,924 - -0.9 479,926 + -0.8 479,936 - -0.8 479,943 - -0.6 479,950 - -0.1 479,960 - -0.4 479,960 - -0.7 480,113 + -1.5 480,155 - +0.3 480,159 + -0.9 480,159 - +1.3 480,159 - +0.5 480,162 - -0.9 480,162 - +0.4 480,167 - +0.4 480,173 + +0.0 480,194 - -1.3 480,261 + -0.2 480,274 - +0.2 480,281 + +1.0 480,281 + -1.7 480,289 - +2.3 480,292 + -1.6 480,292 + +0.1 480,299 - -0.1 480,301 + BBR_RS12170 0.10 -1.6 480,319 - BBR_RS12170 0.15 -0.1 480,357 + BBR_RS12170 0.26 -0.2 480,357 - BBR_RS12170 0.26 +1.1 480,359 - BBR_RS12170 0.26 +0.3 480,359 - BBR_RS12170 0.26 +0.2 480,398 - BBR_RS12170 0.37 -0.3 480,470 - BBR_RS12170 0.57 -0.6 480,483 - BBR_RS12170 0.60 +0.4 480,504 - BBR_RS12170 0.66 +0.3 480,504 - BBR_RS12170 0.66 +0.4 480,512 + BBR_RS12170 0.68 +1.2 480,552 - BBR_RS12170 0.79 -0.1 480,554 + BBR_RS12170 0.80 +0.1 480,602 + +0.0 480,616 - -0.1 480,635 - +2.1 480,643 - -0.6 480,647 + -2.2 480,650 - +1.1 480,650 - +0.1 480,654 + -0.3 480,654 + -1.5 480,654 + +0.5 480,654 + -1.1 480,654 + +1.2 480,660 - -1.0 480,662 - +0.1 480,678 + -0.3 480,689 + +1.2 480,703 + -0.5 480,711 + -0.2 480,723 - BBR_RS12175 0.10 +0.6 480,723 - BBR_RS12175 0.10 -0.1 480,732 - BBR_RS12175 0.11 +0.3 480,770 - BBR_RS12175 0.17 +0.2 480,786 + BBR_RS12175 0.20 -1.2 480,789 - BBR_RS12175 0.20 -0.8 480,789 - BBR_RS12175 0.20 -0.9 480,794 - BBR_RS12175 0.21 -0.8 480,794 - BBR_RS12175 0.21 -0.1 480,794 - BBR_RS12175 0.21 -0.8 480,821 - BBR_RS12175 0.25 -1.4 480,823 + BBR_RS12175 0.25 +0.7 480,823 + BBR_RS12175 0.25 -0.2 480,823 + BBR_RS12175 0.25 +1.4 480,824 - BBR_RS12175 0.25 -1.1 480,831 - BBR_RS12175 0.26 -1.6 480,836 - BBR_RS12175 0.27 -1.1 480,885 + BBR_RS12175 0.34 -3.1 480,885 + BBR_RS12175 0.34 -1.0 480,886 - BBR_RS12175 0.35 -1.6 480,920 - BBR_RS12175 0.40 -0.8 480,957 + BBR_RS12175 0.45 -1.2 480,965 - BBR_RS12175 0.46 -0.6 480,965 - BBR_RS12175 0.46 -0.4 480,975 - BBR_RS12175 0.48 -1.4 480,975 - BBR_RS12175 0.48 -0.6 480,975 - BBR_RS12175 0.48 -0.7 480,975 - BBR_RS12175 0.48 -0.0 480,975 - BBR_RS12175 0.48 -0.1 480,975 - BBR_RS12175 0.48 -1.7 480,975 - BBR_RS12175 0.48 +0.1 480,983 - BBR_RS12175 0.49 -1.0 481,024 + BBR_RS12175 0.55 -0.2 481,053 - BBR_RS12175 0.60 -0.6 481,095 - BBR_RS12175 0.66 -0.4 481,095 - BBR_RS12175 0.66 +0.2 481,095 - BBR_RS12175 0.66 -0.1 481,095 - BBR_RS12175 0.66 -0.6 481,113 + BBR_RS12175 0.69 -0.9 481,252 - BBR_RS12175 0.89 -0.4 481,257 - +2.0 481,329 + +0.5 481,386 - -0.6 481,436 + +2.0 481,450 - +0.4 481,465 + -1.1 481,475 + +0.6 481,475 + +0.6 481,475 + -0.0 481,475 + -1.1 481,475 + +0.4 481,483 - -1.3 481,483 - -0.7 481,483 - +0.6 481,504 - -2.4 481,525 - +0.5 481,536 - BBR_RS12180 0.11 -1.8 481,536 - BBR_RS12180 0.11 +0.3 481,575 - BBR_RS12180 0.14 -1.1 481,579 - BBR_RS12180 0.14 +0.5 481,579 - BBR_RS12180 0.14 -0.5 481,590 + BBR_RS12180 0.15 -0.2 481,598 - BBR_RS12180 0.15 -1.2 481,598 - BBR_RS12180 0.15 -0.5 481,598 - BBR_RS12180 0.15 +1.2 481,598 - BBR_RS12180 0.15 +0.4 481,598 - BBR_RS12180 0.15 +0.7 481,599 + BBR_RS12180 0.15 -1.8 481,630 + BBR_RS12180 0.18 -2.2 481,646 + BBR_RS12180 0.19 +0.4 481,649 + BBR_RS12180 0.19 +0.4 481,654 - BBR_RS12180 0.20 -0.4 481,654 - BBR_RS12180 0.20 -0.4 481,663 + BBR_RS12180 0.20 -0.0 481,663 + BBR_RS12180 0.20 -1.6 481,671 + BBR_RS12180 0.21 -0.5 481,671 - BBR_RS12180 0.21 -1.1 481,672 + BBR_RS12180 0.21 -0.0 481,672 + BBR_RS12180 0.21 +0.3 481,672 - BBR_RS12180 0.21 -0.8 481,685 + BBR_RS12180 0.22 -1.2 481,685 + BBR_RS12180 0.22 +0.8 481,685 - BBR_RS12180 0.22 +1.0 481,693 + BBR_RS12180 0.22 -3.6 481,693 - BBR_RS12180 0.22 -0.5 481,695 + BBR_RS12180 0.23 -0.4 481,695 + BBR_RS12180 0.23 +0.2 481,695 + BBR_RS12180 0.23 -1.4 481,703 - BBR_RS12180 0.23 +1.1 481,703 - BBR_RS12180 0.23 -0.4 481,723 + BBR_RS12180 0.25 -1.6 481,741 - BBR_RS12180 0.26 +0.1 481,743 + BBR_RS12180 0.26 -1.9 481,743 + BBR_RS12180 0.26 +0.9 481,743 + BBR_RS12180 0.26 +0.6 481,751 - BBR_RS12180 0.27 -0.8 481,751 - BBR_RS12180 0.27 -1.8 481,751 - BBR_RS12180 0.27 -0.4 481,751 - BBR_RS12180 0.27 +0.8 481,780 - BBR_RS12180 0.29 +0.0 481,780 - BBR_RS12180 0.29 -1.0 481,813 + BBR_RS12180 0.31 +0.0 481,818 + BBR_RS12180 0.32 +0.3 481,818 + BBR_RS12180 0.32 -0.4 481,821 - BBR_RS12180 0.32 +0.3 481,826 - BBR_RS12180 0.32 -0.1 481,826 - BBR_RS12180 0.32 -2.1 481,826 - BBR_RS12180 0.32 +1.1 481,831 - BBR_RS12180 0.33 +0.2 481,841 - BBR_RS12180 0.33 +1.2 481,857 + BBR_RS12180 0.35 -0.6 481,857 + BBR_RS12180 0.35 -0.5 481,857 + BBR_RS12180 0.35 -0.3 481,857 + BBR_RS12180 0.35 -0.4 481,857 + BBR_RS12180 0.35 -1.1 481,857 + BBR_RS12180 0.35 +0.6 481,857 + BBR_RS12180 0.35 -0.2 481,865 - BBR_RS12180 0.35 -0.3 481,865 - BBR_RS12180 0.35 -1.3 481,865 - BBR_RS12180 0.35 -1.0 481,865 - BBR_RS12180 0.35 -0.9 481,865 - BBR_RS12180 0.35 +0.4 481,865 - BBR_RS12180 0.35 +0.4 481,865 - BBR_RS12180 0.35 -0.5 481,865 - BBR_RS12180 0.35 -0.4 481,865 - BBR_RS12180 0.35 +0.1 481,865 - BBR_RS12180 0.35 -1.2 481,922 - BBR_RS12180 0.40 +1.3 481,935 + BBR_RS12180 0.41 -0.3 481,965 + BBR_RS12180 0.43 -1.3 481,973 - BBR_RS12180 0.43 -0.5 481,973 - BBR_RS12180 0.43 -0.5 481,995 - BBR_RS12180 0.45 +0.2 482,010 - BBR_RS12180 0.46 -0.5 482,010 - BBR_RS12180 0.46 -0.5 482,054 + BBR_RS12180 0.49 -1.3 482,062 - BBR_RS12180 0.50 +0.8 482,076 + BBR_RS12180 0.51 -0.9 482,112 + BBR_RS12180 0.54 -0.6 482,120 - BBR_RS12180 0.54 -0.7 482,120 - BBR_RS12180 0.54 -0.1 482,120 - BBR_RS12180 0.54 -0.2 482,139 + BBR_RS12180 0.56 -1.3 482,139 + BBR_RS12180 0.56 -1.9 482,139 + BBR_RS12180 0.56 +0.4 482,139 + BBR_RS12180 0.56 -0.8 482,147 - BBR_RS12180 0.56 +0.5 482,147 - BBR_RS12180 0.56 +0.1 482,147 - BBR_RS12180 0.56 +0.3 482,147 - BBR_RS12180 0.56 +1.3 482,147 - BBR_RS12180 0.56 -0.7 482,169 + BBR_RS12180 0.58 -0.9 482,170 - BBR_RS12180 0.58 -0.5 482,202 + BBR_RS12180 0.60 +0.1 482,202 + BBR_RS12180 0.60 -0.8 482,214 + BBR_RS12180 0.61 -0.9 482,232 + BBR_RS12180 0.63 +0.1 482,237 + BBR_RS12180 0.63 +0.1 482,240 - BBR_RS12180 0.63 -1.2 482,246 - BBR_RS12180 0.64 -0.0 482,255 - BBR_RS12180 0.64 -0.4 482,303 + BBR_RS12180 0.68 -0.3 482,305 + BBR_RS12180 0.68 +0.0
Or see this region's nucleotide sequence