Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12050 and BBR_RS12055 are separated by 222 nucleotides BBR_RS12055 and BBR_RS12060 are separated by 138 nucleotides BBR_RS12060 and BBR_RS12065 are separated by 147 nucleotides
BBR_RS12050: BBR_RS12050 - hypothetical protein, at 453,108 to 454,112
_RS12050
BBR_RS12055: BBR_RS12055 - thioredoxin, at 454,335 to 455,306
_RS12055
BBR_RS12060: BBR_RS12060 - adenylate cyclase, at 455,445 to 456,071
_RS12060
BBR_RS12065: BBR_RS12065 - tRNA-Arg, at 456,219 to 456,294
_RS12065
Position (kb) 454
455
456 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 454.097 kb on + strand at 454.113 kb on + strand at 454.113 kb on + strand at 454.170 kb on + strand at 454.188 kb on - strand at 454.191 kb on + strand at 454.235 kb on + strand at 454.292 kb on + strand at 454.301 kb on + strand at 454.331 kb on + strand at 454.352 kb on + strand at 454.357 kb on + strand at 454.357 kb on + strand at 454.360 kb on - strand at 454.370 kb on - strand at 454.409 kb on + strand at 454.409 kb on + strand at 454.409 kb on + strand at 454.409 kb on + strand at 454.409 kb on + strand at 454.409 kb on + strand at 454.409 kb on + strand at 454.409 kb on + strand at 454.415 kb on + strand at 454.417 kb on - strand at 454.417 kb on - strand at 454.417 kb on - strand at 454.417 kb on - strand at 454.417 kb on - strand at 454.417 kb on - strand at 454.417 kb on - strand at 454.428 kb on - strand at 454.432 kb on - strand at 454.433 kb on - strand, within BBR_RS12055 at 454.449 kb on + strand, within BBR_RS12055 at 454.473 kb on + strand, within BBR_RS12055 at 454.483 kb on + strand, within BBR_RS12055 at 454.496 kb on + strand, within BBR_RS12055 at 454.497 kb on + strand, within BBR_RS12055 at 454.497 kb on + strand, within BBR_RS12055 at 454.497 kb on + strand, within BBR_RS12055 at 454.497 kb on + strand, within BBR_RS12055 at 454.497 kb on + strand, within BBR_RS12055 at 454.497 kb on + strand, within BBR_RS12055 at 454.497 kb on + strand, within BBR_RS12055 at 454.497 kb on + strand, within BBR_RS12055 at 454.497 kb on + strand, within BBR_RS12055 at 454.505 kb on - strand, within BBR_RS12055 at 454.505 kb on - strand, within BBR_RS12055 at 454.505 kb on - strand, within BBR_RS12055 at 454.529 kb on + strand, within BBR_RS12055 at 454.531 kb on + strand, within BBR_RS12055 at 454.582 kb on + strand, within BBR_RS12055 at 454.666 kb on - strand, within BBR_RS12055 at 454.705 kb on - strand, within BBR_RS12055 at 454.729 kb on - strand, within BBR_RS12055 at 454.735 kb on - strand, within BBR_RS12055 at 454.792 kb on + strand, within BBR_RS12055 at 454.792 kb on + strand, within BBR_RS12055 at 454.797 kb on + strand, within BBR_RS12055 at 454.802 kb on + strand, within BBR_RS12055 at 454.826 kb on + strand, within BBR_RS12055 at 454.861 kb on + strand, within BBR_RS12055 at 454.861 kb on + strand, within BBR_RS12055 at 454.864 kb on - strand, within BBR_RS12055 at 454.864 kb on - strand, within BBR_RS12055 at 454.887 kb on + strand, within BBR_RS12055 at 454.887 kb on + strand, within BBR_RS12055 at 454.907 kb on + strand, within BBR_RS12055 at 454.915 kb on - strand, within BBR_RS12055 at 454.915 kb on - strand, within BBR_RS12055 at 454.915 kb on - strand, within BBR_RS12055 at 454.927 kb on - strand, within BBR_RS12055 at 454.927 kb on - strand, within BBR_RS12055 at 454.959 kb on + strand, within BBR_RS12055 at 454.961 kb on + strand, within BBR_RS12055 at 454.967 kb on - strand, within BBR_RS12055 at 454.978 kb on + strand, within BBR_RS12055 at 454.978 kb on + strand, within BBR_RS12055 at 454.978 kb on + strand, within BBR_RS12055 at 454.986 kb on - strand, within BBR_RS12055 at 454.990 kb on + strand, within BBR_RS12055 at 455.047 kb on + strand, within BBR_RS12055 at 455.047 kb on - strand, within BBR_RS12055 at 455.055 kb on - strand, within BBR_RS12055 at 455.081 kb on - strand, within BBR_RS12055 at 455.093 kb on + strand, within BBR_RS12055 at 455.144 kb on + strand, within BBR_RS12055 at 455.144 kb on + strand, within BBR_RS12055 at 455.154 kb on + strand, within BBR_RS12055 at 455.162 kb on - strand, within BBR_RS12055 at 455.162 kb on - strand, within BBR_RS12055 at 455.187 kb on + strand, within BBR_RS12055 at 455.187 kb on + strand, within BBR_RS12055 at 455.187 kb on + strand, within BBR_RS12055 at 455.189 kb on + strand, within BBR_RS12055 at 455.197 kb on - strand, within BBR_RS12055 at 455.226 kb on + strand at 455.234 kb on - strand at 455.234 kb on - strand at 455.242 kb on + strand at 455.276 kb on - strand at 455.292 kb on + strand at 455.300 kb on - strand at 455.300 kb on - strand at 455.345 kb on - strand at 455.359 kb on - strand at 455.361 kb on + strand at 455.361 kb on + strand at 455.364 kb on + strand at 455.364 kb on + strand at 455.364 kb on + strand at 455.371 kb on + strand at 455.372 kb on - strand at 455.372 kb on - strand at 455.372 kb on - strand at 455.408 kb on + strand at 455.417 kb on - strand at 455.417 kb on - strand at 455.417 kb on - strand at 455.421 kb on + strand at 455.450 kb on - strand at 455.490 kb on + strand at 455.490 kb on + strand at 455.490 kb on + strand at 455.491 kb on + strand at 455.497 kb on + strand at 455.515 kb on - strand, within BBR_RS12060 at 455.521 kb on + strand, within BBR_RS12060 at 455.524 kb on + strand, within BBR_RS12060 at 455.527 kb on + strand, within BBR_RS12060 at 455.590 kb on + strand, within BBR_RS12060 at 455.623 kb on + strand, within BBR_RS12060 at 455.623 kb on + strand, within BBR_RS12060 at 455.624 kb on - strand, within BBR_RS12060 at 455.632 kb on + strand, within BBR_RS12060 at 455.643 kb on + strand, within BBR_RS12060 at 455.654 kb on - strand, within BBR_RS12060 at 455.656 kb on - strand, within BBR_RS12060 at 455.686 kb on + strand, within BBR_RS12060 at 455.686 kb on + strand, within BBR_RS12060 at 455.686 kb on + strand, within BBR_RS12060 at 455.692 kb on - strand, within BBR_RS12060 at 455.699 kb on + strand, within BBR_RS12060 at 455.708 kb on + strand, within BBR_RS12060 at 455.721 kb on - strand, within BBR_RS12060 at 455.729 kb on - strand, within BBR_RS12060 at 455.746 kb on + strand, within BBR_RS12060 at 455.792 kb on + strand, within BBR_RS12060 at 455.797 kb on + strand, within BBR_RS12060 at 455.815 kb on + strand, within BBR_RS12060 at 455.842 kb on + strand, within BBR_RS12060 at 455.842 kb on + strand, within BBR_RS12060 at 455.867 kb on - strand, within BBR_RS12060 at 455.867 kb on - strand, within BBR_RS12060 at 455.894 kb on + strand, within BBR_RS12060 at 455.896 kb on - strand, within BBR_RS12060 at 455.902 kb on - strand, within BBR_RS12060 at 455.917 kb on + strand, within BBR_RS12060 at 455.922 kb on + strand, within BBR_RS12060 at 455.946 kb on + strand, within BBR_RS12060 at 455.947 kb on - strand, within BBR_RS12060 at 455.949 kb on - strand, within BBR_RS12060 at 455.965 kb on - strand, within BBR_RS12060 at 455.975 kb on - strand, within BBR_RS12060 at 455.975 kb on - strand, within BBR_RS12060 at 455.976 kb on + strand, within BBR_RS12060 at 455.981 kb on + strand, within BBR_RS12060 at 456.001 kb on + strand, within BBR_RS12060 at 456.003 kb on + strand, within BBR_RS12060 at 456.053 kb on + strand at 456.087 kb on - strand at 456.117 kb on + strand at 456.142 kb on + strand at 456.152 kb on + strand at 456.152 kb on + strand at 456.157 kb on + strand at 456.160 kb on - strand at 456.160 kb on - strand at 456.165 kb on + strand at 456.185 kb on + strand at 456.185 kb on + strand at 456.185 kb on + strand at 456.196 kb on + strand at 456.197 kb on + strand at 456.197 kb on + strand at 456.204 kb on + strand at 456.204 kb on + strand at 456.215 kb on + strand at 456.215 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 454,097 + -0.3 454,113 + -2.0 454,113 + +0.4 454,170 + -0.4 454,188 - +0.7 454,191 + +1.0 454,235 + +1.0 454,292 + -0.6 454,301 + -0.1 454,331 + -2.0 454,352 + +0.7 454,357 + -0.5 454,357 + +0.0 454,360 - -1.8 454,370 - +0.1 454,409 + -0.2 454,409 + +0.3 454,409 + -0.1 454,409 + -1.6 454,409 + +0.2 454,409 + -1.4 454,409 + -0.2 454,409 + +0.3 454,415 + +0.2 454,417 - -0.8 454,417 - +0.3 454,417 - +0.5 454,417 - +0.6 454,417 - +0.1 454,417 - -1.2 454,417 - -2.0 454,428 - -0.8 454,432 - +0.3 454,433 - BBR_RS12055 0.10 -0.2 454,449 + BBR_RS12055 0.12 -0.2 454,473 + BBR_RS12055 0.14 -0.9 454,483 + BBR_RS12055 0.15 -1.0 454,496 + BBR_RS12055 0.17 -1.2 454,497 + BBR_RS12055 0.17 -2.0 454,497 + BBR_RS12055 0.17 -1.3 454,497 + BBR_RS12055 0.17 -0.1 454,497 + BBR_RS12055 0.17 -2.1 454,497 + BBR_RS12055 0.17 +0.2 454,497 + BBR_RS12055 0.17 -1.2 454,497 + BBR_RS12055 0.17 -1.3 454,497 + BBR_RS12055 0.17 -0.9 454,497 + BBR_RS12055 0.17 +0.1 454,505 - BBR_RS12055 0.17 +0.5 454,505 - BBR_RS12055 0.17 +0.1 454,505 - BBR_RS12055 0.17 -0.9 454,529 + BBR_RS12055 0.20 -0.7 454,531 + BBR_RS12055 0.20 -0.3 454,582 + BBR_RS12055 0.25 -0.0 454,666 - BBR_RS12055 0.34 +0.0 454,705 - BBR_RS12055 0.38 -1.8 454,729 - BBR_RS12055 0.41 -0.8 454,735 - BBR_RS12055 0.41 +0.5 454,792 + BBR_RS12055 0.47 -2.0 454,792 + BBR_RS12055 0.47 -0.7 454,797 + BBR_RS12055 0.48 -0.2 454,802 + BBR_RS12055 0.48 -0.6 454,826 + BBR_RS12055 0.51 +1.2 454,861 + BBR_RS12055 0.54 +0.4 454,861 + BBR_RS12055 0.54 -0.7 454,864 - BBR_RS12055 0.54 -0.3 454,864 - BBR_RS12055 0.54 -0.6 454,887 + BBR_RS12055 0.57 +0.0 454,887 + BBR_RS12055 0.57 -0.6 454,907 + BBR_RS12055 0.59 -0.2 454,915 - BBR_RS12055 0.60 -0.9 454,915 - BBR_RS12055 0.60 -0.4 454,915 - BBR_RS12055 0.60 -1.1 454,927 - BBR_RS12055 0.61 -1.9 454,927 - BBR_RS12055 0.61 -0.6 454,959 + BBR_RS12055 0.64 -0.8 454,961 + BBR_RS12055 0.64 -0.4 454,967 - BBR_RS12055 0.65 +0.3 454,978 + BBR_RS12055 0.66 -1.7 454,978 + BBR_RS12055 0.66 +0.2 454,978 + BBR_RS12055 0.66 -0.4 454,986 - BBR_RS12055 0.67 +0.3 454,990 + BBR_RS12055 0.67 -0.3 455,047 + BBR_RS12055 0.73 -0.8 455,047 - BBR_RS12055 0.73 -0.3 455,055 - BBR_RS12055 0.74 +0.7 455,081 - BBR_RS12055 0.77 -0.2 455,093 + BBR_RS12055 0.78 -1.2 455,144 + BBR_RS12055 0.83 -0.5 455,144 + BBR_RS12055 0.83 -0.7 455,154 + BBR_RS12055 0.84 +1.1 455,162 - BBR_RS12055 0.85 -0.9 455,162 - BBR_RS12055 0.85 +1.6 455,187 + BBR_RS12055 0.88 -1.9 455,187 + BBR_RS12055 0.88 -1.9 455,187 + BBR_RS12055 0.88 -1.9 455,189 + BBR_RS12055 0.88 -0.4 455,197 - BBR_RS12055 0.89 +0.1 455,226 + +1.4 455,234 - -1.1 455,234 - +0.7 455,242 + -0.5 455,276 - -0.9 455,292 + -0.5 455,300 - +0.6 455,300 - -0.0 455,345 - +1.8 455,359 - -1.5 455,361 + +0.5 455,361 + -0.3 455,364 + +0.9 455,364 + -0.3 455,364 + -0.2 455,371 + -1.9 455,372 - -0.6 455,372 - +1.4 455,372 - -1.3 455,408 + -0.3 455,417 - -0.4 455,417 - +0.3 455,417 - +1.0 455,421 + +2.0 455,450 - +0.4 455,490 + -0.2 455,490 + -0.1 455,490 + +0.0 455,491 + +0.8 455,497 + -1.7 455,515 - BBR_RS12060 0.11 -0.4 455,521 + BBR_RS12060 0.12 -0.1 455,524 + BBR_RS12060 0.13 +1.3 455,527 + BBR_RS12060 0.13 +0.8 455,590 + BBR_RS12060 0.23 -0.9 455,623 + BBR_RS12060 0.28 -0.1 455,623 + BBR_RS12060 0.28 +0.1 455,624 - BBR_RS12060 0.29 +0.2 455,632 + BBR_RS12060 0.30 -0.5 455,643 + BBR_RS12060 0.32 -1.3 455,654 - BBR_RS12060 0.33 -0.8 455,656 - BBR_RS12060 0.34 +0.0 455,686 + BBR_RS12060 0.38 -0.6 455,686 + BBR_RS12060 0.38 -0.6 455,686 + BBR_RS12060 0.38 +1.4 455,692 - BBR_RS12060 0.39 -0.4 455,699 + BBR_RS12060 0.41 -0.7 455,708 + BBR_RS12060 0.42 +0.5 455,721 - BBR_RS12060 0.44 +0.6 455,729 - BBR_RS12060 0.45 +0.1 455,746 + BBR_RS12060 0.48 -0.9 455,792 + BBR_RS12060 0.55 +2.2 455,797 + BBR_RS12060 0.56 -0.2 455,815 + BBR_RS12060 0.59 -0.0 455,842 + BBR_RS12060 0.63 -0.4 455,842 + BBR_RS12060 0.63 -0.4 455,867 - BBR_RS12060 0.67 +0.3 455,867 - BBR_RS12060 0.67 +1.0 455,894 + BBR_RS12060 0.72 -0.2 455,896 - BBR_RS12060 0.72 -0.7 455,902 - BBR_RS12060 0.73 -0.1 455,917 + BBR_RS12060 0.75 +0.6 455,922 + BBR_RS12060 0.76 -0.5 455,946 + BBR_RS12060 0.80 +0.3 455,947 - BBR_RS12060 0.80 -0.1 455,949 - BBR_RS12060 0.80 +0.1 455,965 - BBR_RS12060 0.83 -0.0 455,975 - BBR_RS12060 0.85 -0.2 455,975 - BBR_RS12060 0.85 +0.1 455,976 + BBR_RS12060 0.85 -0.0 455,981 + BBR_RS12060 0.85 +0.9 456,001 + BBR_RS12060 0.89 +0.6 456,003 + BBR_RS12060 0.89 +0.3 456,053 + +0.2 456,087 - -1.4 456,117 + -0.4 456,142 + -0.2 456,152 + +0.4 456,152 + -1.2 456,157 + +1.3 456,160 - +0.3 456,160 - -0.0 456,165 + +1.2 456,185 + -0.3 456,185 + -0.3 456,185 + -0.5 456,196 + -0.0 456,197 + -0.6 456,197 + -0.4 456,204 + -1.1 456,204 + +0.1 456,215 + +0.1 456,215 + +3.0
Or see this region's nucleotide sequence