Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS12045

Experiment: Recovery broth with Tiamulin 2e-04 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS12040 and BBR_RS12045 are separated by 101 nucleotidesBBR_RS12045 and BBR_RS12050 overlap by 1 nucleotides BBR_RS12040: BBR_RS12040 - peptidyl-prolyl cis-trans isomerase, at 450,815 to 451,768 _RS12040 BBR_RS12045: BBR_RS12045 - hypothetical protein, at 451,870 to 453,108 _RS12045 BBR_RS12050: BBR_RS12050 - hypothetical protein, at 453,108 to 454,112 _RS12050 Position (kb) 451 452 453 454Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 450.888 kb on + strandat 450.901 kb on - strandat 450.901 kb on - strandat 450.901 kb on - strandat 450.901 kb on - strandat 450.901 kb on - strandat 450.901 kb on - strandat 450.952 kb on - strand, within BBR_RS12040at 450.963 kb on - strand, within BBR_RS12040at 450.975 kb on - strand, within BBR_RS12040at 450.975 kb on - strand, within BBR_RS12040at 450.995 kb on - strand, within BBR_RS12040at 451.001 kb on + strand, within BBR_RS12040at 451.001 kb on + strand, within BBR_RS12040at 451.016 kb on + strand, within BBR_RS12040at 451.019 kb on - strand, within BBR_RS12040at 451.019 kb on - strand, within BBR_RS12040at 451.024 kb on + strand, within BBR_RS12040at 451.026 kb on + strand, within BBR_RS12040at 451.026 kb on + strand, within BBR_RS12040at 451.057 kb on + strand, within BBR_RS12040at 451.102 kb on - strand, within BBR_RS12040at 451.113 kb on + strand, within BBR_RS12040at 451.115 kb on + strand, within BBR_RS12040at 451.121 kb on - strand, within BBR_RS12040at 451.121 kb on - strand, within BBR_RS12040at 451.121 kb on - strand, within BBR_RS12040at 451.121 kb on - strand, within BBR_RS12040at 451.158 kb on - strand, within BBR_RS12040at 451.158 kb on - strand, within BBR_RS12040at 451.158 kb on - strand, within BBR_RS12040at 451.160 kb on + strand, within BBR_RS12040at 451.160 kb on + strand, within BBR_RS12040at 451.160 kb on + strand, within BBR_RS12040at 451.176 kb on + strand, within BBR_RS12040at 451.224 kb on + strand, within BBR_RS12040at 451.224 kb on + strand, within BBR_RS12040at 451.224 kb on + strand, within BBR_RS12040at 451.224 kb on + strand, within BBR_RS12040at 451.226 kb on + strand, within BBR_RS12040at 451.226 kb on + strand, within BBR_RS12040at 451.231 kb on - strand, within BBR_RS12040at 451.231 kb on - strand, within BBR_RS12040at 451.232 kb on - strand, within BBR_RS12040at 451.232 kb on - strand, within BBR_RS12040at 451.234 kb on + strand, within BBR_RS12040at 451.234 kb on + strand, within BBR_RS12040at 451.258 kb on + strand, within BBR_RS12040at 451.265 kb on - strand, within BBR_RS12040at 451.349 kb on - strand, within BBR_RS12040at 451.364 kb on - strand, within BBR_RS12040at 451.374 kb on - strand, within BBR_RS12040at 451.377 kb on + strand, within BBR_RS12040at 451.385 kb on - strand, within BBR_RS12040at 451.455 kb on - strand, within BBR_RS12040at 451.532 kb on - strand, within BBR_RS12040at 451.662 kb on - strand, within BBR_RS12040at 451.670 kb on - strand, within BBR_RS12040at 451.748 kb on - strandat 451.750 kb on + strandat 451.770 kb on + strandat 451.770 kb on + strandat 451.778 kb on - strandat 451.821 kb on - strandat 451.843 kb on + strandat 451.853 kb on - strandat 451.896 kb on + strandat 451.961 kb on + strandat 451.971 kb on + strandat 452.010 kb on + strand, within BBR_RS12045at 452.059 kb on + strand, within BBR_RS12045at 452.071 kb on + strand, within BBR_RS12045at 452.857 kb on + strand, within BBR_RS12045at 452.932 kb on + strand, within BBR_RS12045at 452.932 kb on + strand, within BBR_RS12045at 452.948 kb on + strand, within BBR_RS12045at 452.948 kb on + strand, within BBR_RS12045at 452.950 kb on + strand, within BBR_RS12045at 452.950 kb on + strand, within BBR_RS12045at 452.953 kb on + strand, within BBR_RS12045at 452.958 kb on + strand, within BBR_RS12045at 452.966 kb on - strand, within BBR_RS12045at 453.021 kb on + strandat 453.021 kb on + strandat 453.021 kb on + strandat 453.051 kb on + strandat 453.051 kb on - strandat 453.051 kb on - strandat 453.066 kb on - strandat 453.093 kb on + strandat 453.097 kb on + strandat 453.107 kb on + strandat 453.110 kb on - strandat 454.097 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM
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450,888 + +0.3
450,901 - -0.5
450,901 - +0.1
450,901 - +0.2
450,901 - -0.7
450,901 - +0.5
450,901 - -0.2
450,952 - BBR_RS12040 0.14 -0.7
450,963 - BBR_RS12040 0.16 -1.1
450,975 - BBR_RS12040 0.17 +1.1
450,975 - BBR_RS12040 0.17 +0.6
450,995 - BBR_RS12040 0.19 -0.1
451,001 + BBR_RS12040 0.19 -2.1
451,001 + BBR_RS12040 0.19 -0.4
451,016 + BBR_RS12040 0.21 +0.1
451,019 - BBR_RS12040 0.21 +0.4
451,019 - BBR_RS12040 0.21 -0.5
451,024 + BBR_RS12040 0.22 +0.4
451,026 + BBR_RS12040 0.22 +0.4
451,026 + BBR_RS12040 0.22 +0.9
451,057 + BBR_RS12040 0.25 +0.3
451,102 - BBR_RS12040 0.30 -0.5
451,113 + BBR_RS12040 0.31 +0.1
451,115 + BBR_RS12040 0.31 +0.1
451,121 - BBR_RS12040 0.32 +1.3
451,121 - BBR_RS12040 0.32 +0.6
451,121 - BBR_RS12040 0.32 -0.6
451,121 - BBR_RS12040 0.32 +0.3
451,158 - BBR_RS12040 0.36 -0.1
451,158 - BBR_RS12040 0.36 +0.6
451,158 - BBR_RS12040 0.36 -0.0
451,160 + BBR_RS12040 0.36 +0.5
451,160 + BBR_RS12040 0.36 +0.6
451,160 + BBR_RS12040 0.36 -1.1
451,176 + BBR_RS12040 0.38 +0.2
451,224 + BBR_RS12040 0.43 -0.3
451,224 + BBR_RS12040 0.43 +1.0
451,224 + BBR_RS12040 0.43 +1.1
451,224 + BBR_RS12040 0.43 -0.3
451,226 + BBR_RS12040 0.43 -1.1
451,226 + BBR_RS12040 0.43 +0.9
451,231 - BBR_RS12040 0.44 -0.3
451,231 - BBR_RS12040 0.44 +0.6
451,232 - BBR_RS12040 0.44 +0.8
451,232 - BBR_RS12040 0.44 +0.2
451,234 + BBR_RS12040 0.44 +1.0
451,234 + BBR_RS12040 0.44 -0.2
451,258 + BBR_RS12040 0.46 -0.5
451,265 - BBR_RS12040 0.47 +0.8
451,349 - BBR_RS12040 0.56 +0.7
451,364 - BBR_RS12040 0.58 +3.0
451,374 - BBR_RS12040 0.59 +1.4
451,377 + BBR_RS12040 0.59 +2.0
451,385 - BBR_RS12040 0.60 +2.0
451,455 - BBR_RS12040 0.67 +1.4
451,532 - BBR_RS12040 0.75 -0.3
451,662 - BBR_RS12040 0.89 +3.0
451,670 - BBR_RS12040 0.90 +2.4
451,748 - -0.4
451,750 + +2.0
451,770 + -0.1
451,770 + -0.5
451,778 - -0.1
451,821 - +0.8
451,843 + -1.1
451,853 - -0.9
451,896 + +1.4
451,961 + +2.0
451,971 + +0.3
452,010 + BBR_RS12045 0.11 -1.5
452,059 + BBR_RS12045 0.15 -0.7
452,071 + BBR_RS12045 0.16 -0.1
452,857 + BBR_RS12045 0.80 -3.2
452,932 + BBR_RS12045 0.86 -2.8
452,932 + BBR_RS12045 0.86 -1.0
452,948 + BBR_RS12045 0.87 -0.6
452,948 + BBR_RS12045 0.87 -0.4
452,950 + BBR_RS12045 0.87 -1.1
452,950 + BBR_RS12045 0.87 -1.3
452,953 + BBR_RS12045 0.87 -1.0
452,958 + BBR_RS12045 0.88 -1.3
452,966 - BBR_RS12045 0.88 +1.4
453,021 + -0.9
453,021 + +0.6
453,021 + -0.3
453,051 + -0.9
453,051 - -1.9
453,051 - +0.4
453,066 - +0.0
453,093 + -0.4
453,097 + +0.5
453,107 + +0.0
453,110 - -1.0
454,097 + -0.3

Or see this region's nucleotide sequence