Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10985 and BBR_RS10990 are separated by 44 nucleotides BBR_RS10990 and BBR_RS10995 are separated by 120 nucleotides BBR_RS10995 and BBR_RS20570 overlap by 31 nucleotides BBR_RS20570 and BBR_RS11000 are separated by 0 nucleotides
BBR_RS10985: BBR_RS10985 - DNA polymerase III subunit gamma and tau, at 204,111 to 206,843
_RS10985
BBR_RS10990: BBR_RS10990 - recombination protein RecR, at 206,888 to 207,490
_RS10990
BBR_RS10995: BBR_RS10995 - ATP-binding protein, at 207,611 to 207,934
_RS10995
BBR_RS20570: BBR_RS20570 - hypothetical protein, at 207,904 to 208,176
_RS20570
BBR_RS11000: BBR_RS11000 - class C sortase, at 208,177 to 209,400
_RS11000
Position (kb) 206
207
208 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 206.769 kb on + strand at 206.810 kb on + strand at 206.810 kb on + strand at 206.844 kb on + strand at 206.844 kb on + strand at 206.845 kb on - strand at 206.845 kb on - strand at 206.847 kb on - strand at 206.850 kb on - strand at 206.850 kb on - strand at 206.874 kb on + strand at 206.874 kb on + strand at 206.885 kb on - strand at 206.891 kb on + strand at 206.897 kb on + strand at 206.904 kb on - strand at 206.905 kb on - strand at 206.905 kb on - strand at 206.910 kb on - strand at 206.910 kb on - strand at 206.923 kb on + strand at 206.923 kb on + strand at 206.930 kb on - strand at 206.931 kb on + strand at 206.952 kb on - strand, within BBR_RS10990 at 206.966 kb on + strand, within BBR_RS10990 at 206.979 kb on - strand, within BBR_RS10990 at 207.005 kb on + strand, within BBR_RS10990 at 207.030 kb on - strand, within BBR_RS10990 at 207.048 kb on - strand, within BBR_RS10990 at 207.108 kb on + strand, within BBR_RS10990 at 207.111 kb on - strand, within BBR_RS10990 at 207.116 kb on - strand, within BBR_RS10990 at 207.116 kb on - strand, within BBR_RS10990 at 207.161 kb on + strand, within BBR_RS10990 at 207.162 kb on - strand, within BBR_RS10990 at 207.162 kb on - strand, within BBR_RS10990 at 207.162 kb on - strand, within BBR_RS10990 at 207.162 kb on - strand, within BBR_RS10990 at 207.169 kb on - strand, within BBR_RS10990 at 207.173 kb on + strand, within BBR_RS10990 at 207.183 kb on + strand, within BBR_RS10990 at 207.192 kb on + strand, within BBR_RS10990 at 207.193 kb on - strand, within BBR_RS10990 at 207.198 kb on + strand, within BBR_RS10990 at 207.199 kb on + strand, within BBR_RS10990 at 207.202 kb on + strand, within BBR_RS10990 at 207.206 kb on - strand, within BBR_RS10990 at 207.211 kb on + strand, within BBR_RS10990 at 207.240 kb on + strand, within BBR_RS10990 at 207.248 kb on - strand, within BBR_RS10990 at 207.248 kb on - strand, within BBR_RS10990 at 207.272 kb on - strand, within BBR_RS10990 at 207.272 kb on - strand, within BBR_RS10990 at 207.272 kb on - strand, within BBR_RS10990 at 207.272 kb on - strand, within BBR_RS10990 at 207.274 kb on + strand, within BBR_RS10990 at 207.274 kb on + strand, within BBR_RS10990 at 207.274 kb on + strand, within BBR_RS10990 at 207.274 kb on + strand, within BBR_RS10990 at 207.282 kb on - strand, within BBR_RS10990 at 207.306 kb on - strand, within BBR_RS10990 at 207.317 kb on + strand, within BBR_RS10990 at 207.352 kb on - strand, within BBR_RS10990 at 207.399 kb on - strand, within BBR_RS10990 at 207.447 kb on + strand at 207.485 kb on + strand at 207.486 kb on - strand at 207.498 kb on - strand at 207.504 kb on + strand at 207.510 kb on - strand at 207.510 kb on - strand at 207.510 kb on - strand at 207.510 kb on - strand at 207.512 kb on - strand at 207.569 kb on + strand at 207.572 kb on + strand at 207.574 kb on + strand at 207.577 kb on - strand at 207.580 kb on - strand at 207.595 kb on - strand at 207.595 kb on - strand at 207.595 kb on - strand at 207.641 kb on - strand at 207.642 kb on + strand at 207.642 kb on + strand at 207.642 kb on + strand at 207.650 kb on - strand, within BBR_RS10995 at 207.650 kb on - strand, within BBR_RS10995 at 207.650 kb on - strand, within BBR_RS10995 at 207.659 kb on + strand, within BBR_RS10995 at 207.694 kb on - strand, within BBR_RS10995 at 207.694 kb on - strand, within BBR_RS10995 at 207.698 kb on + strand, within BBR_RS10995 at 207.706 kb on - strand, within BBR_RS10995 at 207.706 kb on - strand, within BBR_RS10995 at 207.706 kb on - strand, within BBR_RS10995 at 207.708 kb on + strand, within BBR_RS10995 at 207.788 kb on + strand, within BBR_RS10995 at 207.803 kb on + strand, within BBR_RS10995 at 207.808 kb on - strand, within BBR_RS10995 at 207.810 kb on + strand, within BBR_RS10995 at 207.810 kb on + strand, within BBR_RS10995 at 207.811 kb on - strand, within BBR_RS10995 at 207.813 kb on - strand, within BBR_RS10995 at 207.818 kb on - strand, within BBR_RS10995 at 207.818 kb on - strand, within BBR_RS10995 at 207.818 kb on - strand, within BBR_RS10995 at 207.848 kb on + strand, within BBR_RS10995 at 207.882 kb on - strand, within BBR_RS10995 at 207.916 kb on - strand at 207.916 kb on - strand at 207.923 kb on + strand at 207.958 kb on + strand, within BBR_RS20570 at 207.958 kb on - strand, within BBR_RS20570 at 207.960 kb on + strand, within BBR_RS20570 at 207.960 kb on + strand, within BBR_RS20570 at 207.975 kb on + strand, within BBR_RS20570 at 207.983 kb on - strand, within BBR_RS20570 at 207.992 kb on + strand, within BBR_RS20570 at 208.002 kb on + strand, within BBR_RS20570 at 208.018 kb on - strand, within BBR_RS20570 at 208.036 kb on + strand, within BBR_RS20570 at 208.036 kb on + strand, within BBR_RS20570 at 208.056 kb on + strand, within BBR_RS20570 at 208.064 kb on + strand, within BBR_RS20570 at 208.064 kb on - strand, within BBR_RS20570 at 208.073 kb on - strand, within BBR_RS20570 at 208.107 kb on - strand, within BBR_RS20570 at 208.122 kb on - strand, within BBR_RS20570 at 208.133 kb on + strand, within BBR_RS20570 at 208.135 kb on + strand, within BBR_RS20570 at 208.153 kb on + strand at 208.205 kb on - strand at 208.213 kb on - strand at 208.225 kb on - strand at 208.230 kb on + strand at 208.230 kb on + strand at 208.234 kb on + strand at 208.234 kb on + strand at 208.244 kb on - strand at 208.281 kb on - strand at 208.292 kb on + strand at 208.292 kb on + strand at 208.302 kb on + strand, within BBR_RS11000 at 208.315 kb on - strand, within BBR_RS11000 at 208.315 kb on - strand, within BBR_RS11000 at 208.315 kb on - strand, within BBR_RS11000 at 208.315 kb on - strand, within BBR_RS11000 at 208.315 kb on - strand, within BBR_RS11000 at 208.331 kb on + strand, within BBR_RS11000 at 208.331 kb on + strand, within BBR_RS11000 at 208.331 kb on + strand, within BBR_RS11000 at 208.331 kb on + strand, within BBR_RS11000 at 208.339 kb on - strand, within BBR_RS11000 at 208.339 kb on - strand, within BBR_RS11000 at 208.341 kb on + strand, within BBR_RS11000 at 208.348 kb on - strand, within BBR_RS11000 at 208.360 kb on - strand, within BBR_RS11000 at 208.372 kb on + strand, within BBR_RS11000 at 208.380 kb on + strand, within BBR_RS11000 at 208.390 kb on + strand, within BBR_RS11000 at 208.398 kb on - strand, within BBR_RS11000 at 208.406 kb on - strand, within BBR_RS11000 at 208.407 kb on + strand, within BBR_RS11000 at 208.407 kb on + strand, within BBR_RS11000 at 208.407 kb on + strand, within BBR_RS11000 at 208.407 kb on + strand, within BBR_RS11000 at 208.407 kb on + strand, within BBR_RS11000 at 208.407 kb on + strand, within BBR_RS11000 at 208.407 kb on + strand, within BBR_RS11000 at 208.407 kb on + strand, within BBR_RS11000 at 208.407 kb on + strand, within BBR_RS11000 at 208.415 kb on - strand, within BBR_RS11000 at 208.415 kb on - strand, within BBR_RS11000 at 208.415 kb on - strand, within BBR_RS11000 at 208.415 kb on - strand, within BBR_RS11000 at 208.415 kb on - strand, within BBR_RS11000 at 208.418 kb on + strand, within BBR_RS11000 at 208.464 kb on + strand, within BBR_RS11000 at 208.465 kb on - strand, within BBR_RS11000 at 208.472 kb on + strand, within BBR_RS11000 at 208.472 kb on + strand, within BBR_RS11000 at 208.472 kb on + strand, within BBR_RS11000 at 208.476 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.479 kb on + strand, within BBR_RS11000 at 208.481 kb on + strand, within BBR_RS11000 at 208.484 kb on + strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.487 kb on - strand, within BBR_RS11000 at 208.489 kb on - strand, within BBR_RS11000
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 206,769 + +0.3 206,810 + -1.7 206,810 + +0.0 206,844 + -0.0 206,844 + +1.1 206,845 - +0.4 206,845 - -0.0 206,847 - +0.3 206,850 - +0.2 206,850 - +0.1 206,874 + +0.2 206,874 + +0.4 206,885 - -0.3 206,891 + -0.9 206,897 + -0.8 206,904 - +0.8 206,905 - +0.4 206,905 - -1.3 206,910 - -0.6 206,910 - +0.4 206,923 + -1.3 206,923 + +0.1 206,930 - +0.6 206,931 + +1.5 206,952 - BBR_RS10990 0.11 +1.2 206,966 + BBR_RS10990 0.13 +2.1 206,979 - BBR_RS10990 0.15 +0.5 207,005 + BBR_RS10990 0.19 -0.5 207,030 - BBR_RS10990 0.24 -0.9 207,048 - BBR_RS10990 0.27 -1.1 207,108 + BBR_RS10990 0.36 +1.4 207,111 - BBR_RS10990 0.37 -0.5 207,116 - BBR_RS10990 0.38 -0.6 207,116 - BBR_RS10990 0.38 +1.5 207,161 + BBR_RS10990 0.45 -1.8 207,162 - BBR_RS10990 0.45 -0.2 207,162 - BBR_RS10990 0.45 -0.6 207,162 - BBR_RS10990 0.45 -1.6 207,162 - BBR_RS10990 0.45 -1.1 207,169 - BBR_RS10990 0.47 -0.7 207,173 + BBR_RS10990 0.47 -1.5 207,183 + BBR_RS10990 0.49 +1.6 207,192 + BBR_RS10990 0.50 +0.9 207,193 - BBR_RS10990 0.51 -0.1 207,198 + BBR_RS10990 0.51 -0.6 207,199 + BBR_RS10990 0.52 -0.4 207,202 + BBR_RS10990 0.52 -0.3 207,206 - BBR_RS10990 0.53 +0.2 207,211 + BBR_RS10990 0.54 +0.4 207,240 + BBR_RS10990 0.58 -0.4 207,248 - BBR_RS10990 0.60 -0.2 207,248 - BBR_RS10990 0.60 +1.1 207,272 - BBR_RS10990 0.64 -1.1 207,272 - BBR_RS10990 0.64 -1.7 207,272 - BBR_RS10990 0.64 +1.1 207,272 - BBR_RS10990 0.64 -0.6 207,274 + BBR_RS10990 0.64 +1.5 207,274 + BBR_RS10990 0.64 +0.2 207,274 + BBR_RS10990 0.64 -0.5 207,274 + BBR_RS10990 0.64 +0.9 207,282 - BBR_RS10990 0.65 +0.2 207,306 - BBR_RS10990 0.69 -0.9 207,317 + BBR_RS10990 0.71 -0.8 207,352 - BBR_RS10990 0.77 -0.4 207,399 - BBR_RS10990 0.85 +2.1 207,447 + +0.9 207,485 + +0.2 207,486 - -0.9 207,498 - -0.1 207,504 + -0.2 207,510 - +0.5 207,510 - -0.2 207,510 - +0.3 207,510 - -0.4 207,512 - -0.1 207,569 + -0.1 207,572 + -0.2 207,574 + -1.2 207,577 - +3.8 207,580 - +0.3 207,595 - -0.8 207,595 - +0.7 207,595 - -1.2 207,641 - +1.1 207,642 + -0.5 207,642 + -0.4 207,642 + -0.1 207,650 - BBR_RS10995 0.12 +0.6 207,650 - BBR_RS10995 0.12 -0.1 207,650 - BBR_RS10995 0.12 +0.6 207,659 + BBR_RS10995 0.15 +0.1 207,694 - BBR_RS10995 0.26 +0.1 207,694 - BBR_RS10995 0.26 +0.4 207,698 + BBR_RS10995 0.27 -0.6 207,706 - BBR_RS10995 0.29 -0.8 207,706 - BBR_RS10995 0.29 +0.9 207,706 - BBR_RS10995 0.29 +0.9 207,708 + BBR_RS10995 0.30 +0.7 207,788 + BBR_RS10995 0.55 +0.6 207,803 + BBR_RS10995 0.59 -0.2 207,808 - BBR_RS10995 0.61 -0.4 207,810 + BBR_RS10995 0.61 +1.2 207,810 + BBR_RS10995 0.61 -0.0 207,811 - BBR_RS10995 0.62 -0.1 207,813 - BBR_RS10995 0.62 -0.2 207,818 - BBR_RS10995 0.64 -0.8 207,818 - BBR_RS10995 0.64 +0.5 207,818 - BBR_RS10995 0.64 +0.4 207,848 + BBR_RS10995 0.73 -1.4 207,882 - BBR_RS10995 0.84 +0.2 207,916 - +0.4 207,916 - -0.0 207,923 + +0.0 207,958 + BBR_RS20570 0.20 -0.1 207,958 - BBR_RS20570 0.20 +0.9 207,960 + BBR_RS20570 0.21 +0.3 207,960 + BBR_RS20570 0.21 -0.1 207,975 + BBR_RS20570 0.26 +0.2 207,983 - BBR_RS20570 0.29 +2.2 207,992 + BBR_RS20570 0.32 -0.0 208,002 + BBR_RS20570 0.36 +1.7 208,018 - BBR_RS20570 0.42 -0.4 208,036 + BBR_RS20570 0.48 -0.4 208,036 + BBR_RS20570 0.48 -0.5 208,056 + BBR_RS20570 0.56 -1.7 208,064 + BBR_RS20570 0.59 -0.0 208,064 - BBR_RS20570 0.59 +0.2 208,073 - BBR_RS20570 0.62 -2.4 208,107 - BBR_RS20570 0.74 +0.9 208,122 - BBR_RS20570 0.80 -0.4 208,133 + BBR_RS20570 0.84 -0.5 208,135 + BBR_RS20570 0.85 +1.2 208,153 + +0.3 208,205 - +1.5 208,213 - -0.7 208,225 - +0.3 208,230 + -0.4 208,230 + -1.6 208,234 + +0.5 208,234 + -0.4 208,244 - -1.4 208,281 - +0.4 208,292 + +0.4 208,292 + -0.0 208,302 + BBR_RS11000 0.10 +0.4 208,315 - BBR_RS11000 0.11 -0.3 208,315 - BBR_RS11000 0.11 -0.2 208,315 - BBR_RS11000 0.11 +0.5 208,315 - BBR_RS11000 0.11 -0.8 208,315 - BBR_RS11000 0.11 -1.0 208,331 + BBR_RS11000 0.13 +0.5 208,331 + BBR_RS11000 0.13 -0.1 208,331 + BBR_RS11000 0.13 +0.1 208,331 + BBR_RS11000 0.13 -0.6 208,339 - BBR_RS11000 0.13 +0.3 208,339 - BBR_RS11000 0.13 +0.4 208,341 + BBR_RS11000 0.13 -1.5 208,348 - BBR_RS11000 0.14 +1.4 208,360 - BBR_RS11000 0.15 -0.1 208,372 + BBR_RS11000 0.16 -0.4 208,380 + BBR_RS11000 0.17 +0.5 208,390 + BBR_RS11000 0.17 +0.5 208,398 - BBR_RS11000 0.18 -1.1 208,406 - BBR_RS11000 0.19 -0.2 208,407 + BBR_RS11000 0.19 -1.2 208,407 + BBR_RS11000 0.19 -0.2 208,407 + BBR_RS11000 0.19 -0.9 208,407 + BBR_RS11000 0.19 -0.4 208,407 + BBR_RS11000 0.19 +0.5 208,407 + BBR_RS11000 0.19 +0.7 208,407 + BBR_RS11000 0.19 +0.9 208,407 + BBR_RS11000 0.19 -0.3 208,407 + BBR_RS11000 0.19 -0.7 208,415 - BBR_RS11000 0.19 +1.6 208,415 - BBR_RS11000 0.19 +0.1 208,415 - BBR_RS11000 0.19 -0.1 208,415 - BBR_RS11000 0.19 -0.0 208,415 - BBR_RS11000 0.19 -1.2 208,418 + BBR_RS11000 0.20 -1.8 208,464 + BBR_RS11000 0.23 -0.4 208,465 - BBR_RS11000 0.24 -0.3 208,472 + BBR_RS11000 0.24 -0.1 208,472 + BBR_RS11000 0.24 -0.6 208,472 + BBR_RS11000 0.24 +0.9 208,476 + BBR_RS11000 0.24 +0.4 208,479 + BBR_RS11000 0.25 -1.9 208,479 + BBR_RS11000 0.25 +0.0 208,479 + BBR_RS11000 0.25 -0.3 208,479 + BBR_RS11000 0.25 +0.5 208,479 + BBR_RS11000 0.25 -0.6 208,479 + BBR_RS11000 0.25 +0.1 208,479 + BBR_RS11000 0.25 +0.6 208,479 + BBR_RS11000 0.25 -0.2 208,479 + BBR_RS11000 0.25 +0.5 208,479 + BBR_RS11000 0.25 +0.1 208,479 + BBR_RS11000 0.25 -0.5 208,479 + BBR_RS11000 0.25 +1.0 208,481 + BBR_RS11000 0.25 -0.4 208,484 + BBR_RS11000 0.25 -0.0 208,487 - BBR_RS11000 0.25 +1.0 208,487 - BBR_RS11000 0.25 +0.5 208,487 - BBR_RS11000 0.25 +0.0 208,487 - BBR_RS11000 0.25 -0.5 208,487 - BBR_RS11000 0.25 -0.2 208,487 - BBR_RS11000 0.25 +2.4 208,487 - BBR_RS11000 0.25 -0.8 208,487 - BBR_RS11000 0.25 -0.3 208,487 - BBR_RS11000 0.25 +0.3 208,487 - BBR_RS11000 0.25 +0.2 208,487 - BBR_RS11000 0.25 +0.9 208,489 - BBR_RS11000 0.25 -0.4
Or see this region's nucleotide sequence