Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10615 and BBR_RS10620 overlap by 4 nucleotides BBR_RS10620 and BBR_RS10625 are separated by 4 nucleotides BBR_RS10625 and BBR_RS10630 are separated by 30 nucleotides
BBR_RS10615: BBR_RS10615 - FtsW/RodA/SpoVE family cell cycle protein, at 99,519 to 101,126
_RS10615
BBR_RS10620: BBR_RS10620 - phosphoprotein phosphatase, at 101,123 to 102,742
_RS10620
BBR_RS10625: BBR_RS10625 - FHA domain-containing protein, at 102,747 to 103,271
_RS10625
BBR_RS10630: BBR_RS10630 - DUF2662 domain-containing protein, at 103,302 to 104,003
_RS10630
Position (kb) 101
102
103 Strain fitness (log2 ratio) -2
-1
0
1
2 at 101.178 kb on + strand at 101.238 kb on - strand at 101.245 kb on - strand at 101.325 kb on - strand, within BBR_RS10620 at 101.325 kb on - strand, within BBR_RS10620 at 101.325 kb on - strand, within BBR_RS10620 at 101.325 kb on - strand, within BBR_RS10620 at 101.412 kb on - strand, within BBR_RS10620 at 101.424 kb on - strand, within BBR_RS10620 at 101.424 kb on - strand, within BBR_RS10620 at 101.439 kb on - strand, within BBR_RS10620 at 101.489 kb on - strand, within BBR_RS10620 at 101.490 kb on - strand, within BBR_RS10620 at 101.491 kb on - strand, within BBR_RS10620 at 101.523 kb on + strand, within BBR_RS10620 at 101.536 kb on - strand, within BBR_RS10620 at 102.763 kb on - strand at 102.808 kb on - strand, within BBR_RS10625 at 102.808 kb on - strand, within BBR_RS10625 at 102.810 kb on - strand, within BBR_RS10625 at 102.860 kb on - strand, within BBR_RS10625 at 103.275 kb on - strand at 103.275 kb on - strand at 103.275 kb on - strand at 103.275 kb on - strand at 103.275 kb on - strand at 103.275 kb on - strand at 103.275 kb on - strand at 103.307 kb on - strand at 103.307 kb on - strand at 103.307 kb on - strand at 103.312 kb on - strand at 103.332 kb on - strand at 103.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 101,178 + -1.6 101,238 - +0.0 101,245 - -0.3 101,325 - BBR_RS10620 0.12 -0.4 101,325 - BBR_RS10620 0.12 -0.2 101,325 - BBR_RS10620 0.12 +0.6 101,325 - BBR_RS10620 0.12 -0.6 101,412 - BBR_RS10620 0.18 -2.1 101,424 - BBR_RS10620 0.19 -2.0 101,424 - BBR_RS10620 0.19 -1.3 101,439 - BBR_RS10620 0.20 -1.3 101,489 - BBR_RS10620 0.23 +1.5 101,490 - BBR_RS10620 0.23 -1.6 101,491 - BBR_RS10620 0.23 +1.5 101,523 + BBR_RS10620 0.25 +2.1 101,536 - BBR_RS10620 0.25 -2.4 102,763 - -0.5 102,808 - BBR_RS10625 0.12 -1.5 102,808 - BBR_RS10625 0.12 -0.9 102,810 - BBR_RS10625 0.12 -1.4 102,860 - BBR_RS10625 0.22 +1.5 103,275 - +0.4 103,275 - -0.9 103,275 - -0.4 103,275 - -0.2 103,275 - +0.0 103,275 - +0.2 103,275 - -0.9 103,307 - -1.2 103,307 - -2.3 103,307 - -1.9 103,312 - -0.9 103,332 - -2.3 103,337 - -1.8
Or see this region's nucleotide sequence