Experiment: Recovery broth with Tiamulin 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10260 and BBR_RS10265 are separated by 24 nucleotides BBR_RS10265 and BBR_RS10270 overlap by 4 nucleotides BBR_RS10270 and BBR_RS10275 are separated by 182 nucleotides
BBR_RS10260: BBR_RS10260 - DNA polymerase III subunit beta, at 2,043 to 3,167
_RS10260
BBR_RS10265: BBR_RS10265 - DNA replication and repair protein RecF, at 3,192 to 4,349
_RS10265
BBR_RS10270: BBR_RS10270 - DUF721 domain-containing protein, at 4,346 to 4,816
_RS10270
BBR_RS10275: BBR_RS10275 - DNA topoisomerase (ATP-hydrolyzing) subunit B, at 4,999 to 7,089
_RS10275
Position (kb) 3
4
5 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3.176 kb on + strand at 3.176 kb on + strand at 3.178 kb on + strand at 3.178 kb on + strand at 3.233 kb on + strand at 3.235 kb on + strand at 3.239 kb on + strand at 3.241 kb on - strand at 3.294 kb on + strand at 3.308 kb on + strand, within BBR_RS10265 at 3.308 kb on + strand, within BBR_RS10265 at 3.308 kb on + strand, within BBR_RS10265 at 3.308 kb on + strand, within BBR_RS10265 at 3.326 kb on + strand, within BBR_RS10265 at 3.326 kb on + strand, within BBR_RS10265 at 3.337 kb on + strand, within BBR_RS10265 at 3.352 kb on + strand, within BBR_RS10265 at 3.421 kb on + strand, within BBR_RS10265 at 3.434 kb on - strand, within BBR_RS10265 at 3.439 kb on + strand, within BBR_RS10265 at 3.482 kb on + strand, within BBR_RS10265 at 3.503 kb on + strand, within BBR_RS10265 at 3.503 kb on + strand, within BBR_RS10265 at 3.542 kb on + strand, within BBR_RS10265 at 3.549 kb on + strand, within BBR_RS10265 at 3.557 kb on + strand, within BBR_RS10265 at 3.566 kb on + strand, within BBR_RS10265 at 3.579 kb on + strand, within BBR_RS10265 at 3.597 kb on + strand, within BBR_RS10265 at 3.608 kb on + strand, within BBR_RS10265 at 3.616 kb on - strand, within BBR_RS10265 at 3.620 kb on + strand, within BBR_RS10265 at 3.625 kb on + strand, within BBR_RS10265 at 3.628 kb on - strand, within BBR_RS10265 at 3.664 kb on + strand, within BBR_RS10265 at 3.684 kb on + strand, within BBR_RS10265 at 3.684 kb on + strand, within BBR_RS10265 at 3.693 kb on - strand, within BBR_RS10265 at 3.707 kb on + strand, within BBR_RS10265 at 3.707 kb on + strand, within BBR_RS10265 at 3.717 kb on - strand, within BBR_RS10265 at 3.725 kb on - strand, within BBR_RS10265 at 3.735 kb on + strand, within BBR_RS10265 at 3.766 kb on - strand, within BBR_RS10265 at 3.767 kb on + strand, within BBR_RS10265 at 3.775 kb on - strand, within BBR_RS10265 at 3.786 kb on + strand, within BBR_RS10265 at 3.786 kb on + strand, within BBR_RS10265 at 3.786 kb on + strand, within BBR_RS10265 at 3.788 kb on - strand, within BBR_RS10265 at 3.793 kb on + strand, within BBR_RS10265 at 3.794 kb on - strand, within BBR_RS10265 at 3.794 kb on - strand, within BBR_RS10265 at 3.824 kb on + strand, within BBR_RS10265 at 3.850 kb on - strand, within BBR_RS10265 at 3.852 kb on + strand, within BBR_RS10265 at 3.852 kb on + strand, within BBR_RS10265 at 3.852 kb on + strand, within BBR_RS10265 at 3.852 kb on + strand, within BBR_RS10265 at 3.857 kb on + strand, within BBR_RS10265 at 3.866 kb on + strand, within BBR_RS10265 at 3.866 kb on + strand, within BBR_RS10265 at 3.870 kb on + strand, within BBR_RS10265 at 3.879 kb on + strand, within BBR_RS10265 at 3.886 kb on + strand, within BBR_RS10265 at 3.887 kb on - strand, within BBR_RS10265 at 3.887 kb on - strand, within BBR_RS10265 at 3.917 kb on - strand, within BBR_RS10265 at 3.917 kb on - strand, within BBR_RS10265 at 3.917 kb on - strand, within BBR_RS10265 at 3.938 kb on - strand, within BBR_RS10265 at 3.938 kb on - strand, within BBR_RS10265 at 3.959 kb on + strand, within BBR_RS10265 at 3.959 kb on + strand, within BBR_RS10265 at 3.964 kb on + strand, within BBR_RS10265 at 3.967 kb on - strand, within BBR_RS10265 at 3.973 kb on - strand, within BBR_RS10265 at 3.977 kb on + strand, within BBR_RS10265 at 4.070 kb on + strand, within BBR_RS10265 at 4.070 kb on + strand, within BBR_RS10265 at 4.172 kb on + strand, within BBR_RS10265 at 4.221 kb on + strand, within BBR_RS10265 at 4.224 kb on - strand, within BBR_RS10265 at 4.229 kb on + strand, within BBR_RS10265 at 4.229 kb on - strand, within BBR_RS10265 at 4.264 kb on + strand at 4.264 kb on + strand at 4.264 kb on + strand at 4.264 kb on + strand at 4.272 kb on - strand at 4.309 kb on + strand at 4.309 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.319 kb on + strand at 4.321 kb on + strand at 4.327 kb on - strand at 4.327 kb on - strand at 4.327 kb on - strand at 4.327 kb on - strand at 4.327 kb on - strand at 4.327 kb on - strand at 4.327 kb on - strand at 4.327 kb on - strand at 4.327 kb on - strand at 4.327 kb on - strand at 4.327 kb on - strand at 4.331 kb on + strand at 4.331 kb on + strand at 4.341 kb on - strand at 4.345 kb on + strand at 4.351 kb on - strand at 4.351 kb on - strand at 4.359 kb on - strand at 4.768 kb on + strand, within BBR_RS10270 at 4.817 kb on + strand at 4.817 kb on + strand at 4.819 kb on + strand at 4.819 kb on + strand at 4.819 kb on + strand at 4.819 kb on + strand at 4.819 kb on + strand at 4.827 kb on - strand at 4.827 kb on - strand at 4.827 kb on - strand at 4.869 kb on - strand at 4.968 kb on + strand at 5.028 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 2e-04 mM remove 3,176 + -0.3 3,176 + -0.7 3,178 + -0.6 3,178 + -0.2 3,233 + +0.7 3,235 + +0.7 3,239 + +0.1 3,241 - +1.5 3,294 + -0.5 3,308 + BBR_RS10265 0.10 -0.7 3,308 + BBR_RS10265 0.10 -1.2 3,308 + BBR_RS10265 0.10 +0.2 3,308 + BBR_RS10265 0.10 -0.1 3,326 + BBR_RS10265 0.12 -0.8 3,326 + BBR_RS10265 0.12 -0.8 3,337 + BBR_RS10265 0.13 +0.5 3,352 + BBR_RS10265 0.14 -0.5 3,421 + BBR_RS10265 0.20 -2.0 3,434 - BBR_RS10265 0.21 -0.5 3,439 + BBR_RS10265 0.21 -1.0 3,482 + BBR_RS10265 0.25 -0.8 3,503 + BBR_RS10265 0.27 -1.9 3,503 + BBR_RS10265 0.27 -0.1 3,542 + BBR_RS10265 0.30 -0.9 3,549 + BBR_RS10265 0.31 -0.4 3,557 + BBR_RS10265 0.32 -1.4 3,566 + BBR_RS10265 0.32 +0.3 3,579 + BBR_RS10265 0.33 -1.0 3,597 + BBR_RS10265 0.35 +0.2 3,608 + BBR_RS10265 0.36 +0.9 3,616 - BBR_RS10265 0.37 +0.7 3,620 + BBR_RS10265 0.37 -2.8 3,625 + BBR_RS10265 0.37 -0.6 3,628 - BBR_RS10265 0.38 -0.4 3,664 + BBR_RS10265 0.41 -0.8 3,684 + BBR_RS10265 0.42 +2.1 3,684 + BBR_RS10265 0.42 -0.8 3,693 - BBR_RS10265 0.43 +0.8 3,707 + BBR_RS10265 0.44 -0.5 3,707 + BBR_RS10265 0.44 +1.2 3,717 - BBR_RS10265 0.45 +0.2 3,725 - BBR_RS10265 0.46 +1.9 3,735 + BBR_RS10265 0.47 -0.8 3,766 - BBR_RS10265 0.50 -0.0 3,767 + BBR_RS10265 0.50 +0.2 3,775 - BBR_RS10265 0.50 -0.6 3,786 + BBR_RS10265 0.51 -1.2 3,786 + BBR_RS10265 0.51 -1.8 3,786 + BBR_RS10265 0.51 -0.3 3,788 - BBR_RS10265 0.51 +0.6 3,793 + BBR_RS10265 0.52 +0.2 3,794 - BBR_RS10265 0.52 -0.3 3,794 - BBR_RS10265 0.52 +0.1 3,824 + BBR_RS10265 0.55 +0.1 3,850 - BBR_RS10265 0.57 +1.5 3,852 + BBR_RS10265 0.57 -0.2 3,852 + BBR_RS10265 0.57 -0.0 3,852 + BBR_RS10265 0.57 -1.9 3,852 + BBR_RS10265 0.57 -2.4 3,857 + BBR_RS10265 0.57 -0.4 3,866 + BBR_RS10265 0.58 -0.6 3,866 + BBR_RS10265 0.58 -1.4 3,870 + BBR_RS10265 0.59 +0.1 3,879 + BBR_RS10265 0.59 +0.2 3,886 + BBR_RS10265 0.60 +3.1 3,887 - BBR_RS10265 0.60 -1.2 3,887 - BBR_RS10265 0.60 -0.6 3,917 - BBR_RS10265 0.63 +0.7 3,917 - BBR_RS10265 0.63 -0.0 3,917 - BBR_RS10265 0.63 +1.1 3,938 - BBR_RS10265 0.64 -1.6 3,938 - BBR_RS10265 0.64 -1.6 3,959 + BBR_RS10265 0.66 +1.0 3,959 + BBR_RS10265 0.66 -0.3 3,964 + BBR_RS10265 0.67 -0.4 3,967 - BBR_RS10265 0.67 +1.6 3,973 - BBR_RS10265 0.67 +0.4 3,977 + BBR_RS10265 0.68 +0.1 4,070 + BBR_RS10265 0.76 -0.7 4,070 + BBR_RS10265 0.76 -0.7 4,172 + BBR_RS10265 0.85 -2.2 4,221 + BBR_RS10265 0.89 -0.8 4,224 - BBR_RS10265 0.89 -0.3 4,229 + BBR_RS10265 0.90 +0.1 4,229 - BBR_RS10265 0.90 +0.2 4,264 + +0.1 4,264 + -0.4 4,264 + -0.5 4,264 + +0.2 4,272 - +0.6 4,309 + -0.7 4,309 + -1.0 4,319 + +1.0 4,319 + -0.2 4,319 + +0.2 4,319 + -0.0 4,319 + -0.1 4,319 + +1.3 4,319 + +1.0 4,319 + +0.1 4,319 + -1.8 4,319 + +0.4 4,319 + +0.7 4,319 + -0.7 4,319 + +0.2 4,319 + +1.2 4,319 + -0.2 4,319 + +0.6 4,319 + +0.5 4,319 + +0.3 4,319 + -1.2 4,319 + -0.5 4,319 + -1.0 4,319 + -0.2 4,319 + +0.3 4,319 + -1.3 4,319 + -0.4 4,319 + +0.4 4,319 + +3.1 4,319 + -0.6 4,319 + -0.5 4,321 + +0.4 4,327 - -2.0 4,327 - -1.4 4,327 - +1.0 4,327 - -0.8 4,327 - -0.2 4,327 - +0.6 4,327 - +0.1 4,327 - -0.2 4,327 - -0.7 4,327 - +0.6 4,327 - +0.5 4,331 + -0.7 4,331 + +0.4 4,341 - +0.7 4,345 + +0.7 4,351 - -0.6 4,351 - -1.5 4,359 - +0.1 4,768 + BBR_RS10270 0.90 +0.2 4,817 + +0.1 4,817 + +0.1 4,819 + +0.1 4,819 + -0.6 4,819 + -0.2 4,819 + +1.1 4,819 + -0.5 4,827 - +0.3 4,827 - -1.1 4,827 - -0.4 4,869 - -0.4 4,968 + -2.3 5,028 + -2.6
Or see this region's nucleotide sequence