Experiment: Nalidixic 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS22035 and ABZR86_RS22040 are separated by 59 nucleotides ABZR86_RS22040 and ABZR86_RS22045 overlap by 4 nucleotides ABZR86_RS22045 and ABZR86_RS22050 are separated by 106 nucleotides
ABZR86_RS22035: ABZR86_RS22035 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 368,681 to 370,063
_RS22035
ABZR86_RS22040: ABZR86_RS22040 - lipoprotein insertase outer membrane protein LolB, at 370,123 to 370,731
_RS22040
ABZR86_RS22045: ABZR86_RS22045 - tetratricopeptide repeat protein, at 370,728 to 372,464
_RS22045
ABZR86_RS22050: ABZR86_RS22050 - glutamyl-tRNA reductase, at 372,571 to 373,845
_RS22050
Position (kb) 370
371
372
373 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 369.958 kb on - strand at 370.026 kb on - strand at 370.057 kb on - strand at 370.057 kb on - strand at 370.107 kb on + strand at 370.124 kb on + strand at 370.124 kb on + strand at 370.125 kb on - strand at 370.933 kb on + strand, within ABZR86_RS22045 at 371.047 kb on + strand, within ABZR86_RS22045 at 371.047 kb on + strand, within ABZR86_RS22045 at 371.048 kb on - strand, within ABZR86_RS22045 at 371.048 kb on - strand, within ABZR86_RS22045 at 371.143 kb on + strand, within ABZR86_RS22045 at 371.143 kb on + strand, within ABZR86_RS22045 at 371.143 kb on + strand, within ABZR86_RS22045 at 371.161 kb on + strand, within ABZR86_RS22045 at 371.245 kb on + strand, within ABZR86_RS22045 at 371.245 kb on + strand, within ABZR86_RS22045 at 371.245 kb on + strand, within ABZR86_RS22045 at 371.245 kb on + strand, within ABZR86_RS22045 at 371.246 kb on - strand, within ABZR86_RS22045 at 371.246 kb on - strand, within ABZR86_RS22045 at 371.246 kb on - strand, within ABZR86_RS22045 at 371.246 kb on - strand, within ABZR86_RS22045 at 371.246 kb on - strand, within ABZR86_RS22045 at 371.260 kb on - strand, within ABZR86_RS22045 at 371.267 kb on - strand, within ABZR86_RS22045 at 371.267 kb on - strand, within ABZR86_RS22045 at 371.299 kb on + strand, within ABZR86_RS22045 at 371.423 kb on - strand, within ABZR86_RS22045 at 371.423 kb on - strand, within ABZR86_RS22045 at 371.527 kb on + strand, within ABZR86_RS22045 at 371.587 kb on + strand, within ABZR86_RS22045 at 371.587 kb on + strand, within ABZR86_RS22045 at 371.587 kb on + strand, within ABZR86_RS22045 at 371.588 kb on - strand, within ABZR86_RS22045 at 371.588 kb on - strand, within ABZR86_RS22045 at 371.638 kb on + strand, within ABZR86_RS22045 at 371.638 kb on + strand, within ABZR86_RS22045 at 371.639 kb on - strand, within ABZR86_RS22045 at 371.639 kb on - strand, within ABZR86_RS22045 at 371.639 kb on - strand, within ABZR86_RS22045 at 371.639 kb on - strand, within ABZR86_RS22045 at 371.668 kb on + strand, within ABZR86_RS22045 at 371.669 kb on - strand, within ABZR86_RS22045 at 371.755 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.779 kb on + strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.780 kb on - strand, within ABZR86_RS22045 at 371.834 kb on - strand, within ABZR86_RS22045 at 372.223 kb on + strand, within ABZR86_RS22045 at 372.223 kb on + strand, within ABZR86_RS22045 at 372.224 kb on - strand, within ABZR86_RS22045 at 372.467 kb on + strand at 372.467 kb on + strand at 372.468 kb on - strand at 372.468 kb on - strand at 372.468 kb on - strand at 372.468 kb on - strand at 372.487 kb on + strand at 372.487 kb on + strand at 372.487 kb on + strand at 372.487 kb on + strand at 372.527 kb on + strand at 372.527 kb on + strand at 372.527 kb on + strand at 372.527 kb on + strand at 372.528 kb on - strand at 372.532 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.04 mg/ml remove 369,958 - +0.3 370,026 - +0.1 370,057 - +0.2 370,057 - +2.2 370,107 + -0.6 370,124 + -0.6 370,124 + -0.5 370,125 - +1.4 370,933 + ABZR86_RS22045 0.12 -2.4 371,047 + ABZR86_RS22045 0.18 -1.5 371,047 + ABZR86_RS22045 0.18 -3.5 371,048 - ABZR86_RS22045 0.18 -0.7 371,048 - ABZR86_RS22045 0.18 -0.2 371,143 + ABZR86_RS22045 0.24 -1.6 371,143 + ABZR86_RS22045 0.24 -0.8 371,143 + ABZR86_RS22045 0.24 -2.0 371,161 + ABZR86_RS22045 0.25 -1.7 371,245 + ABZR86_RS22045 0.30 -1.9 371,245 + ABZR86_RS22045 0.30 -2.5 371,245 + ABZR86_RS22045 0.30 -2.5 371,245 + ABZR86_RS22045 0.30 -2.3 371,246 - ABZR86_RS22045 0.30 -0.6 371,246 - ABZR86_RS22045 0.30 -1.2 371,246 - ABZR86_RS22045 0.30 -2.3 371,246 - ABZR86_RS22045 0.30 -1.3 371,246 - ABZR86_RS22045 0.30 -0.7 371,260 - ABZR86_RS22045 0.31 -1.3 371,267 - ABZR86_RS22045 0.31 -1.6 371,267 - ABZR86_RS22045 0.31 -1.2 371,299 + ABZR86_RS22045 0.33 -1.2 371,423 - ABZR86_RS22045 0.40 -0.7 371,423 - ABZR86_RS22045 0.40 -0.9 371,527 + ABZR86_RS22045 0.46 -1.5 371,587 + ABZR86_RS22045 0.49 -1.6 371,587 + ABZR86_RS22045 0.49 -1.3 371,587 + ABZR86_RS22045 0.49 -1.6 371,588 - ABZR86_RS22045 0.50 -0.7 371,588 - ABZR86_RS22045 0.50 -1.0 371,638 + ABZR86_RS22045 0.52 -0.4 371,638 + ABZR86_RS22045 0.52 -1.4 371,639 - ABZR86_RS22045 0.52 -2.6 371,639 - ABZR86_RS22045 0.52 -0.3 371,639 - ABZR86_RS22045 0.52 -0.1 371,639 - ABZR86_RS22045 0.52 +0.5 371,668 + ABZR86_RS22045 0.54 -3.4 371,669 - ABZR86_RS22045 0.54 -2.2 371,755 + ABZR86_RS22045 0.59 -0.9 371,779 + ABZR86_RS22045 0.61 -3.5 371,779 + ABZR86_RS22045 0.61 -3.5 371,779 + ABZR86_RS22045 0.61 -3.1 371,779 + ABZR86_RS22045 0.61 -3.5 371,779 + ABZR86_RS22045 0.61 -1.3 371,779 + ABZR86_RS22045 0.61 -2.2 371,779 + ABZR86_RS22045 0.61 -2.4 371,779 + ABZR86_RS22045 0.61 -1.6 371,779 + ABZR86_RS22045 0.61 -1.4 371,779 + ABZR86_RS22045 0.61 -2.5 371,779 + ABZR86_RS22045 0.61 -2.0 371,780 - ABZR86_RS22045 0.61 -0.4 371,780 - ABZR86_RS22045 0.61 -0.7 371,780 - ABZR86_RS22045 0.61 +0.0 371,780 - ABZR86_RS22045 0.61 -0.4 371,780 - ABZR86_RS22045 0.61 -1.3 371,780 - ABZR86_RS22045 0.61 -1.2 371,780 - ABZR86_RS22045 0.61 -2.2 371,780 - ABZR86_RS22045 0.61 -0.8 371,780 - ABZR86_RS22045 0.61 -3.5 371,780 - ABZR86_RS22045 0.61 -1.5 371,780 - ABZR86_RS22045 0.61 -2.2 371,780 - ABZR86_RS22045 0.61 -0.8 371,780 - ABZR86_RS22045 0.61 -1.3 371,834 - ABZR86_RS22045 0.64 -0.5 372,223 + ABZR86_RS22045 0.86 -3.3 372,223 + ABZR86_RS22045 0.86 -1.4 372,224 - ABZR86_RS22045 0.86 -1.4 372,467 + -1.5 372,467 + -2.1 372,468 - -0.2 372,468 - -2.1 372,468 - +0.1 372,468 - +0.1 372,487 + -1.8 372,487 + -0.9 372,487 + +0.3 372,487 + -0.4 372,527 + -0.0 372,527 + +0.6 372,527 + -0.3 372,527 + +0.6 372,528 - +1.6 372,532 + +1.1
Or see this region's nucleotide sequence