Experiment: Nalidixic 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS01525 and ABZR86_RS01530 overlap by 1 nucleotides ABZR86_RS01530 and ABZR86_RS01535 are separated by 187 nucleotides ABZR86_RS01535 and ABZR86_RS01540 are separated by 89 nucleotides
ABZR86_RS01525: ABZR86_RS01525 - ATP-binding protein, at 332,793 to 334,202
_RS01525
ABZR86_RS01530: ABZR86_RS01530 - response regulator transcription factor, at 334,202 to 334,867
_RS01530
ABZR86_RS01535: ABZR86_RS01535 - (2Fe-2S)-binding protein, at 335,055 to 335,606
_RS01535
ABZR86_RS01540: ABZR86_RS01540 - MipA/OmpV family protein, at 335,696 to 336,451
_RS01540
Position (kb)
334
335 Strain fitness (log2 ratio)
-1
0
1 at 333.594 kb on - strand, within ABZR86_RS01525 at 334.104 kb on + strand at 334.188 kb on + strand at 334.287 kb on + strand, within ABZR86_RS01530 at 334.287 kb on + strand, within ABZR86_RS01530 at 334.287 kb on + strand, within ABZR86_RS01530 at 334.287 kb on + strand, within ABZR86_RS01530 at 334.288 kb on - strand, within ABZR86_RS01530 at 334.288 kb on - strand, within ABZR86_RS01530 at 334.288 kb on - strand, within ABZR86_RS01530 at 334.288 kb on - strand, within ABZR86_RS01530 at 334.572 kb on + strand, within ABZR86_RS01530 at 334.656 kb on + strand, within ABZR86_RS01530 at 334.732 kb on - strand, within ABZR86_RS01530 at 334.732 kb on - strand, within ABZR86_RS01530 at 334.732 kb on - strand, within ABZR86_RS01530 at 334.858 kb on + strand at 334.859 kb on - strand at 334.912 kb on + strand at 334.912 kb on + strand at 334.913 kb on - strand at 334.913 kb on - strand at 334.937 kb on + strand at 334.937 kb on + strand at 334.942 kb on - strand at 335.318 kb on - strand, within ABZR86_RS01535 at 335.319 kb on + strand, within ABZR86_RS01535 at 335.319 kb on + strand, within ABZR86_RS01535 at 335.319 kb on + strand, within ABZR86_RS01535 at 335.320 kb on - strand, within ABZR86_RS01535 at 335.320 kb on - strand, within ABZR86_RS01535 at 335.656 kb on + strand at 335.656 kb on + strand at 335.657 kb on - strand at 335.657 kb on - strand at 335.657 kb on - strand at 335.657 kb on - strand at 335.657 kb on - strand at 335.657 kb on - strand at 335.657 kb on - strand at 335.843 kb on + strand, within ABZR86_RS01540
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.04 mg/ml remove 333,594 - ABZR86_RS01525 0.57 +1.3 334,104 + -1.4 334,188 + -0.2 334,287 + ABZR86_RS01530 0.13 -0.0 334,287 + ABZR86_RS01530 0.13 +0.2 334,287 + ABZR86_RS01530 0.13 +0.4 334,287 + ABZR86_RS01530 0.13 -0.2 334,288 - ABZR86_RS01530 0.13 -0.0 334,288 - ABZR86_RS01530 0.13 +0.1 334,288 - ABZR86_RS01530 0.13 -0.8 334,288 - ABZR86_RS01530 0.13 +0.1 334,572 + ABZR86_RS01530 0.56 -0.3 334,656 + ABZR86_RS01530 0.68 -0.4 334,732 - ABZR86_RS01530 0.80 -0.2 334,732 - ABZR86_RS01530 0.80 -0.6 334,732 - ABZR86_RS01530 0.80 -0.6 334,858 + -1.4 334,859 - -0.4 334,912 + -1.4 334,912 + -0.2 334,913 - +0.5 334,913 - +0.4 334,937 + +0.8 334,937 + -0.2 334,942 - -1.1 335,318 - ABZR86_RS01535 0.48 -0.1 335,319 + ABZR86_RS01535 0.48 -0.8 335,319 + ABZR86_RS01535 0.48 +0.4 335,319 + ABZR86_RS01535 0.48 +0.3 335,320 - ABZR86_RS01535 0.48 +0.3 335,320 - ABZR86_RS01535 0.48 +0.2 335,656 + -0.2 335,656 + -0.1 335,657 - -0.4 335,657 - -0.5 335,657 - -1.0 335,657 - -1.1 335,657 - +0.1 335,657 - -0.0 335,657 - +0.2 335,843 + ABZR86_RS01540 0.19 +0.6
Or see this region's nucleotide sequence