Experiment: Nalidixic 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS01410 and ABZR86_RS01415 are separated by 365 nucleotides ABZR86_RS01415 and ABZR86_RS01420 overlap by 8 nucleotides ABZR86_RS01420 and ABZR86_RS01425 are separated by 26 nucleotides
ABZR86_RS01410: ABZR86_RS01410 - hypothetical protein, at 302,569 to 303,219
_RS01410
ABZR86_RS01415: ABZR86_RS01415 - histidine kinase, at 303,585 to 304,829
_RS01415
ABZR86_RS01420: ABZR86_RS01420 - LytTR family DNA-binding domain-containing protein, at 304,822 to 305,589
_RS01420
ABZR86_RS01425: ABZR86_RS01425 - hypothetical protein, at 305,616 to 305,963
_RS01425
Position (kb) 303
304
305 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 302.878 kb on + strand, within ABZR86_RS01410 at 302.879 kb on - strand, within ABZR86_RS01410 at 302.936 kb on + strand, within ABZR86_RS01410 at 302.936 kb on + strand, within ABZR86_RS01410 at 302.937 kb on - strand, within ABZR86_RS01410 at 303.038 kb on + strand, within ABZR86_RS01410 at 303.057 kb on + strand, within ABZR86_RS01410 at 303.057 kb on + strand, within ABZR86_RS01410 at 303.277 kb on + strand at 303.278 kb on - strand at 303.278 kb on - strand at 303.292 kb on + strand at 303.293 kb on - strand at 303.293 kb on - strand at 303.293 kb on - strand at 303.293 kb on - strand at 303.293 kb on - strand at 303.293 kb on - strand at 303.299 kb on - strand at 303.398 kb on + strand at 303.398 kb on + strand at 303.398 kb on + strand at 303.399 kb on - strand at 303.399 kb on - strand at 303.399 kb on - strand at 303.399 kb on - strand at 303.402 kb on + strand at 303.402 kb on + strand at 303.403 kb on - strand at 303.403 kb on - strand at 303.403 kb on - strand at 303.403 kb on - strand at 303.403 kb on - strand at 303.403 kb on - strand at 303.403 kb on - strand at 303.403 kb on - strand at 303.403 kb on - strand at 303.403 kb on - strand at 303.404 kb on + strand at 303.404 kb on + strand at 303.546 kb on + strand at 303.546 kb on + strand at 303.547 kb on - strand at 303.679 kb on - strand at 303.679 kb on - strand at 304.311 kb on + strand, within ABZR86_RS01415 at 304.311 kb on + strand, within ABZR86_RS01415 at 304.311 kb on + strand, within ABZR86_RS01415 at 304.311 kb on + strand, within ABZR86_RS01415 at 304.312 kb on - strand, within ABZR86_RS01415 at 304.312 kb on - strand, within ABZR86_RS01415 at 304.312 kb on - strand, within ABZR86_RS01415 at 304.369 kb on - strand, within ABZR86_RS01415 at 304.369 kb on - strand, within ABZR86_RS01415 at 304.369 kb on - strand, within ABZR86_RS01415 at 304.444 kb on - strand, within ABZR86_RS01415 at 304.444 kb on - strand, within ABZR86_RS01415 at 304.734 kb on + strand at 304.827 kb on + strand at 304.827 kb on + strand at 304.827 kb on + strand at 304.827 kb on + strand at 304.827 kb on + strand at 304.827 kb on + strand at 304.828 kb on - strand at 304.828 kb on - strand at 304.828 kb on - strand at 304.828 kb on - strand at 304.828 kb on - strand at 305.083 kb on + strand, within ABZR86_RS01420 at 305.084 kb on - strand, within ABZR86_RS01420 at 305.119 kb on + strand, within ABZR86_RS01420 at 305.120 kb on - strand, within ABZR86_RS01420 at 305.120 kb on - strand, within ABZR86_RS01420 at 305.332 kb on + strand, within ABZR86_RS01420 at 305.332 kb on + strand, within ABZR86_RS01420 at 305.333 kb on - strand, within ABZR86_RS01420 at 305.333 kb on - strand, within ABZR86_RS01420 at 305.353 kb on + strand, within ABZR86_RS01420 at 305.353 kb on + strand, within ABZR86_RS01420 at 305.353 kb on + strand, within ABZR86_RS01420 at 305.353 kb on + strand, within ABZR86_RS01420 at 305.353 kb on + strand, within ABZR86_RS01420 at 305.354 kb on - strand, within ABZR86_RS01420 at 305.354 kb on - strand, within ABZR86_RS01420 at 305.354 kb on - strand, within ABZR86_RS01420 at 305.627 kb on - strand at 305.627 kb on - strand at 305.627 kb on - strand at 305.627 kb on - strand at 305.641 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.04 mg/ml remove 302,878 + ABZR86_RS01410 0.47 -0.3 302,879 - ABZR86_RS01410 0.48 -0.0 302,936 + ABZR86_RS01410 0.56 -0.0 302,936 + ABZR86_RS01410 0.56 +0.7 302,937 - ABZR86_RS01410 0.57 +0.8 303,038 + ABZR86_RS01410 0.72 +0.3 303,057 + ABZR86_RS01410 0.75 -0.2 303,057 + ABZR86_RS01410 0.75 +0.4 303,277 + -1.4 303,278 - -0.2 303,278 - -1.1 303,292 + +0.2 303,293 - +0.3 303,293 - -0.3 303,293 - +0.3 303,293 - -0.2 303,293 - -1.4 303,293 - -0.3 303,299 - +0.8 303,398 + -0.6 303,398 + -1.0 303,398 + -0.4 303,399 - -0.6 303,399 - -1.1 303,399 - -1.0 303,399 - -0.7 303,402 + -1.1 303,402 + -0.9 303,403 - +0.3 303,403 - -0.7 303,403 - -0.4 303,403 - -0.5 303,403 - -1.2 303,403 - +0.6 303,403 - -0.0 303,403 - -1.3 303,403 - -0.6 303,403 - -0.2 303,404 + +1.0 303,404 + +0.1 303,546 + +0.6 303,546 + +0.9 303,547 - -0.2 303,679 - +0.3 303,679 - -0.0 304,311 + ABZR86_RS01415 0.58 +1.0 304,311 + ABZR86_RS01415 0.58 +0.6 304,311 + ABZR86_RS01415 0.58 +0.1 304,311 + ABZR86_RS01415 0.58 +0.1 304,312 - ABZR86_RS01415 0.58 -0.7 304,312 - ABZR86_RS01415 0.58 +0.4 304,312 - ABZR86_RS01415 0.58 -0.7 304,369 - ABZR86_RS01415 0.63 +0.5 304,369 - ABZR86_RS01415 0.63 +1.9 304,369 - ABZR86_RS01415 0.63 +0.3 304,444 - ABZR86_RS01415 0.69 -0.6 304,444 - ABZR86_RS01415 0.69 -0.7 304,734 + -0.4 304,827 + -0.7 304,827 + +0.5 304,827 + -0.5 304,827 + -0.4 304,827 + -0.5 304,827 + +0.1 304,828 - +0.5 304,828 - -0.1 304,828 - +0.5 304,828 - +0.9 304,828 - -1.3 305,083 + ABZR86_RS01420 0.34 +0.0 305,084 - ABZR86_RS01420 0.34 -0.4 305,119 + ABZR86_RS01420 0.39 -0.0 305,120 - ABZR86_RS01420 0.39 -2.8 305,120 - ABZR86_RS01420 0.39 +0.1 305,332 + ABZR86_RS01420 0.66 +0.1 305,332 + ABZR86_RS01420 0.66 +0.4 305,333 - ABZR86_RS01420 0.67 +0.4 305,333 - ABZR86_RS01420 0.67 -0.7 305,353 + ABZR86_RS01420 0.69 -0.8 305,353 + ABZR86_RS01420 0.69 -0.3 305,353 + ABZR86_RS01420 0.69 -1.5 305,353 + ABZR86_RS01420 0.69 +1.7 305,353 + ABZR86_RS01420 0.69 +0.4 305,354 - ABZR86_RS01420 0.69 +0.4 305,354 - ABZR86_RS01420 0.69 -0.7 305,354 - ABZR86_RS01420 0.69 -0.9 305,627 - +0.3 305,627 - -0.2 305,627 - +0.4 305,627 - -0.2 305,641 - +0.5
Or see this region's nucleotide sequence