Experiment: Polymyxin B sulfate 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pys and Synpcc7942_1985 are separated by 32 nucleotides Synpcc7942_1985 and pqqL overlap by 8 nucleotides pqqL and rpaC are separated by 165 nucleotides
Synpcc7942_1984: pys - phytoene synthase, at 2,054,486 to 2,055,412
pys
Synpcc7942_1985: Synpcc7942_1985 - hypothetical protein, at 2,055,445 to 2,056,338
_1985
Synpcc7942_1986: pqqL - processing protease, at 2,056,331 to 2,057,653
pqqL
Synpcc7942_1987: rpaC - hypothetical protein, at 2,057,819 to 2,058,133
rpaC
Position (kb)
2056
2057
2058 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2055.488 kb on - strand at 2055.493 kb on - strand at 2055.501 kb on - strand at 2055.547 kb on - strand, within Synpcc7942_1985 at 2055.633 kb on - strand, within Synpcc7942_1985 at 2055.670 kb on + strand, within Synpcc7942_1985 at 2055.745 kb on + strand, within Synpcc7942_1985 at 2055.755 kb on + strand, within Synpcc7942_1985 at 2055.763 kb on - strand, within Synpcc7942_1985 at 2055.763 kb on - strand, within Synpcc7942_1985 at 2055.763 kb on - strand, within Synpcc7942_1985 at 2055.801 kb on + strand, within Synpcc7942_1985 at 2055.801 kb on + strand, within Synpcc7942_1985 at 2055.801 kb on + strand, within Synpcc7942_1985 at 2055.801 kb on + strand, within Synpcc7942_1985 at 2055.809 kb on - strand, within Synpcc7942_1985 at 2055.809 kb on - strand, within Synpcc7942_1985 at 2055.816 kb on - strand, within Synpcc7942_1985 at 2055.827 kb on + strand at 2055.839 kb on + strand, within Synpcc7942_1985 at 2055.839 kb on + strand, within Synpcc7942_1985 at 2055.892 kb on - strand, within Synpcc7942_1985 at 2055.952 kb on + strand, within Synpcc7942_1985 at 2055.952 kb on + strand, within Synpcc7942_1985 at 2055.960 kb on - strand, within Synpcc7942_1985 at 2055.960 kb on - strand, within Synpcc7942_1985 at 2055.960 kb on - strand, within Synpcc7942_1985 at 2055.960 kb on - strand, within Synpcc7942_1985 at 2055.961 kb on - strand, within Synpcc7942_1985 at 2055.970 kb on - strand, within Synpcc7942_1985 at 2055.975 kb on - strand, within Synpcc7942_1985 at 2056.005 kb on + strand, within Synpcc7942_1985 at 2056.040 kb on - strand, within Synpcc7942_1985 at 2056.042 kb on + strand, within Synpcc7942_1985 at 2056.106 kb on - strand, within Synpcc7942_1985 at 2056.122 kb on - strand, within Synpcc7942_1985 at 2056.131 kb on + strand, within Synpcc7942_1985 at 2056.131 kb on + strand, within Synpcc7942_1985 at 2056.191 kb on + strand, within Synpcc7942_1985 at 2056.202 kb on + strand, within Synpcc7942_1985 at 2056.215 kb on + strand, within Synpcc7942_1985 at 2056.237 kb on + strand, within Synpcc7942_1985 at 2056.238 kb on + strand, within Synpcc7942_1985 at 2056.243 kb on + strand, within Synpcc7942_1985 at 2056.250 kb on + strand at 2056.276 kb on - strand at 2056.289 kb on + strand at 2056.289 kb on + strand at 2056.297 kb on - strand at 2056.297 kb on - strand at 2056.297 kb on - strand at 2056.297 kb on - strand at 2056.299 kb on - strand at 2056.383 kb on + strand at 2056.391 kb on - strand at 2056.391 kb on - strand at 2056.470 kb on - strand, within pqqL at 2056.474 kb on - strand, within pqqL at 2056.474 kb on - strand, within pqqL at 2056.487 kb on - strand, within pqqL at 2056.538 kb on + strand, within pqqL at 2056.570 kb on - strand, within pqqL at 2056.578 kb on - strand, within pqqL at 2056.578 kb on - strand, within pqqL at 2056.592 kb on - strand, within pqqL at 2056.607 kb on - strand, within pqqL at 2056.632 kb on + strand, within pqqL at 2056.640 kb on - strand, within pqqL at 2056.642 kb on + strand, within pqqL at 2056.652 kb on - strand, within pqqL at 2056.698 kb on - strand, within pqqL at 2056.724 kb on + strand, within pqqL at 2056.724 kb on + strand, within pqqL at 2056.732 kb on - strand, within pqqL at 2056.734 kb on + strand, within pqqL at 2056.735 kb on - strand, within pqqL at 2056.761 kb on - strand, within pqqL at 2056.780 kb on - strand, within pqqL at 2056.796 kb on - strand, within pqqL at 2056.800 kb on + strand, within pqqL at 2056.800 kb on + strand, within pqqL at 2056.806 kb on + strand, within pqqL at 2056.806 kb on + strand, within pqqL at 2056.808 kb on - strand, within pqqL at 2056.842 kb on + strand, within pqqL at 2056.848 kb on - strand, within pqqL at 2056.867 kb on + strand, within pqqL at 2056.875 kb on - strand, within pqqL at 2056.920 kb on - strand, within pqqL at 2056.926 kb on + strand, within pqqL at 2056.934 kb on - strand, within pqqL at 2056.936 kb on + strand, within pqqL at 2056.949 kb on - strand, within pqqL at 2056.971 kb on - strand, within pqqL at 2057.004 kb on + strand, within pqqL at 2057.009 kb on + strand, within pqqL at 2057.012 kb on - strand, within pqqL at 2057.033 kb on - strand, within pqqL at 2057.085 kb on - strand, within pqqL at 2057.101 kb on + strand, within pqqL at 2057.115 kb on - strand, within pqqL at 2057.144 kb on - strand, within pqqL at 2057.145 kb on - strand, within pqqL at 2057.150 kb on - strand, within pqqL at 2057.150 kb on - strand, within pqqL at 2057.294 kb on + strand, within pqqL at 2057.310 kb on - strand, within pqqL at 2057.310 kb on - strand, within pqqL at 2057.321 kb on - strand, within pqqL at 2057.325 kb on + strand, within pqqL at 2057.333 kb on - strand, within pqqL at 2057.338 kb on - strand, within pqqL at 2057.338 kb on - strand, within pqqL at 2057.346 kb on + strand, within pqqL at 2057.387 kb on - strand, within pqqL at 2057.420 kb on - strand, within pqqL at 2057.465 kb on - strand, within pqqL at 2057.494 kb on + strand, within pqqL at 2057.502 kb on - strand, within pqqL at 2057.502 kb on - strand, within pqqL at 2057.571 kb on + strand at 2057.625 kb on - strand at 2057.634 kb on - strand at 2057.639 kb on + strand at 2057.666 kb on - strand at 2057.687 kb on + strand at 2057.710 kb on + strand at 2057.742 kb on - strand at 2057.824 kb on - strand at 2057.919 kb on - strand, within rpaC at 2057.924 kb on + strand, within rpaC at 2057.932 kb on - strand, within rpaC at 2057.938 kb on - strand, within rpaC at 2057.940 kb on + strand, within rpaC at 2057.948 kb on - strand, within rpaC at 2057.948 kb on - strand, within rpaC at 2057.976 kb on + strand, within rpaC at 2058.039 kb on + strand, within rpaC at 2058.044 kb on - strand, within rpaC at 2058.172 kb on + strand at 2058.179 kb on - strand at 2058.200 kb on - strand at 2058.247 kb on + strand at 2058.277 kb on + strand at 2058.304 kb on - strand at 2058.368 kb on + strand at 2058.403 kb on + strand at 2058.420 kb on - strand at 2058.422 kb on + strand at 2058.456 kb on - strand at 2058.472 kb on - strand at 2058.472 kb on - strand at 2058.479 kb on - strand at 2058.486 kb on - strand at 2058.488 kb on + strand at 2058.496 kb on + strand at 2058.501 kb on + strand at 2058.504 kb on - strand at 2058.518 kb on + strand at 2058.610 kb on - strand at 2058.638 kb on + strand at 2058.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.2 mg/ml remove 2,055,488 - -0.3 2,055,493 - +0.5 2,055,501 - -1.3 2,055,547 - Synpcc7942_1985 0.11 +0.1 2,055,633 - Synpcc7942_1985 0.21 -0.9 2,055,670 + Synpcc7942_1985 0.25 -0.3 2,055,745 + Synpcc7942_1985 0.34 +0.8 2,055,755 + Synpcc7942_1985 0.35 +2.0 2,055,763 - Synpcc7942_1985 0.36 -0.8 2,055,763 - Synpcc7942_1985 0.36 -0.7 2,055,763 - Synpcc7942_1985 0.36 -1.3 2,055,801 + Synpcc7942_1985 0.40 -1.3 2,055,801 + Synpcc7942_1985 0.40 +0.0 2,055,801 + Synpcc7942_1985 0.40 +1.7 2,055,801 + Synpcc7942_1985 0.40 -2.3 2,055,809 - Synpcc7942_1985 0.41 +0.7 2,055,809 - Synpcc7942_1985 0.41 +0.5 2,055,816 - Synpcc7942_1985 0.41 +0.5 2,055,827 + -1.3 2,055,839 + Synpcc7942_1985 0.44 -1.3 2,055,839 + Synpcc7942_1985 0.44 -1.3 2,055,892 - Synpcc7942_1985 0.50 +2.8 2,055,952 + Synpcc7942_1985 0.57 +1.0 2,055,952 + Synpcc7942_1985 0.57 +0.3 2,055,960 - Synpcc7942_1985 0.58 +4.7 2,055,960 - Synpcc7942_1985 0.58 +1.0 2,055,960 - Synpcc7942_1985 0.58 -4.3 2,055,960 - Synpcc7942_1985 0.58 +1.1 2,055,961 - Synpcc7942_1985 0.58 -0.0 2,055,970 - Synpcc7942_1985 0.59 +2.1 2,055,975 - Synpcc7942_1985 0.59 -0.1 2,056,005 + Synpcc7942_1985 0.63 +1.0 2,056,040 - Synpcc7942_1985 0.67 +2.1 2,056,042 + Synpcc7942_1985 0.67 -1.0 2,056,106 - Synpcc7942_1985 0.74 -1.5 2,056,122 - Synpcc7942_1985 0.76 +0.5 2,056,131 + Synpcc7942_1985 0.77 +1.7 2,056,131 + Synpcc7942_1985 0.77 +0.0 2,056,191 + Synpcc7942_1985 0.83 +0.8 2,056,202 + Synpcc7942_1985 0.85 +0.3 2,056,215 + Synpcc7942_1985 0.86 -2.9 2,056,237 + Synpcc7942_1985 0.89 -1.3 2,056,238 + Synpcc7942_1985 0.89 -2.1 2,056,243 + Synpcc7942_1985 0.89 +0.0 2,056,250 + +2.1 2,056,276 - -0.9 2,056,289 + +1.3 2,056,289 + +0.2 2,056,297 - +2.7 2,056,297 - +1.1 2,056,297 - +1.0 2,056,297 - +0.0 2,056,299 - -1.3 2,056,383 + +2.8 2,056,391 - +2.3 2,056,391 - +2.4 2,056,470 - pqqL Synpcc7942_1986 0.11 +3.8 2,056,474 - pqqL Synpcc7942_1986 0.11 +0.8 2,056,474 - pqqL Synpcc7942_1986 0.11 +1.1 2,056,487 - pqqL Synpcc7942_1986 0.12 +1.9 2,056,538 + pqqL Synpcc7942_1986 0.16 +1.9 2,056,570 - pqqL Synpcc7942_1986 0.18 -1.9 2,056,578 - pqqL Synpcc7942_1986 0.19 +3.5 2,056,578 - pqqL Synpcc7942_1986 0.19 +2.4 2,056,592 - pqqL Synpcc7942_1986 0.20 +2.0 2,056,607 - pqqL Synpcc7942_1986 0.21 -0.5 2,056,632 + pqqL Synpcc7942_1986 0.23 +2.1 2,056,640 - pqqL Synpcc7942_1986 0.23 +3.4 2,056,642 + pqqL Synpcc7942_1986 0.24 +3.2 2,056,652 - pqqL Synpcc7942_1986 0.24 +1.8 2,056,698 - pqqL Synpcc7942_1986 0.28 +2.4 2,056,724 + pqqL Synpcc7942_1986 0.30 +1.7 2,056,724 + pqqL Synpcc7942_1986 0.30 +0.5 2,056,732 - pqqL Synpcc7942_1986 0.30 +3.3 2,056,734 + pqqL Synpcc7942_1986 0.30 +0.0 2,056,735 - pqqL Synpcc7942_1986 0.31 +2.9 2,056,761 - pqqL Synpcc7942_1986 0.33 +1.9 2,056,780 - pqqL Synpcc7942_1986 0.34 +1.7 2,056,796 - pqqL Synpcc7942_1986 0.35 +2.2 2,056,800 + pqqL Synpcc7942_1986 0.35 +2.7 2,056,800 + pqqL Synpcc7942_1986 0.35 +2.4 2,056,806 + pqqL Synpcc7942_1986 0.36 +3.3 2,056,806 + pqqL Synpcc7942_1986 0.36 +2.1 2,056,808 - pqqL Synpcc7942_1986 0.36 +2.0 2,056,842 + pqqL Synpcc7942_1986 0.39 +2.3 2,056,848 - pqqL Synpcc7942_1986 0.39 +0.3 2,056,867 + pqqL Synpcc7942_1986 0.41 +1.2 2,056,875 - pqqL Synpcc7942_1986 0.41 -2.3 2,056,920 - pqqL Synpcc7942_1986 0.45 +4.3 2,056,926 + pqqL Synpcc7942_1986 0.45 +3.7 2,056,934 - pqqL Synpcc7942_1986 0.46 +1.9 2,056,936 + pqqL Synpcc7942_1986 0.46 +2.9 2,056,949 - pqqL Synpcc7942_1986 0.47 +2.8 2,056,971 - pqqL Synpcc7942_1986 0.48 +3.4 2,057,004 + pqqL Synpcc7942_1986 0.51 +2.1 2,057,009 + pqqL Synpcc7942_1986 0.51 +1.7 2,057,012 - pqqL Synpcc7942_1986 0.51 +1.5 2,057,033 - pqqL Synpcc7942_1986 0.53 +3.5 2,057,085 - pqqL Synpcc7942_1986 0.57 +1.5 2,057,101 + pqqL Synpcc7942_1986 0.58 +3.3 2,057,115 - pqqL Synpcc7942_1986 0.59 +0.5 2,057,144 - pqqL Synpcc7942_1986 0.61 +1.9 2,057,145 - pqqL Synpcc7942_1986 0.62 +1.6 2,057,150 - pqqL Synpcc7942_1986 0.62 +1.5 2,057,150 - pqqL Synpcc7942_1986 0.62 +1.1 2,057,294 + pqqL Synpcc7942_1986 0.73 +2.9 2,057,310 - pqqL Synpcc7942_1986 0.74 +1.5 2,057,310 - pqqL Synpcc7942_1986 0.74 +4.3 2,057,321 - pqqL Synpcc7942_1986 0.75 +3.2 2,057,325 + pqqL Synpcc7942_1986 0.75 -0.7 2,057,333 - pqqL Synpcc7942_1986 0.76 +2.3 2,057,338 - pqqL Synpcc7942_1986 0.76 +2.7 2,057,338 - pqqL Synpcc7942_1986 0.76 +5.0 2,057,346 + pqqL Synpcc7942_1986 0.77 +2.6 2,057,387 - pqqL Synpcc7942_1986 0.80 +3.1 2,057,420 - pqqL Synpcc7942_1986 0.82 +1.5 2,057,465 - pqqL Synpcc7942_1986 0.86 +2.5 2,057,494 + pqqL Synpcc7942_1986 0.88 +0.9 2,057,502 - pqqL Synpcc7942_1986 0.89 +1.7 2,057,502 - pqqL Synpcc7942_1986 0.89 +0.3 2,057,571 + +2.7 2,057,625 - +0.1 2,057,634 - +1.8 2,057,639 + +0.7 2,057,666 - +0.4 2,057,687 + +0.7 2,057,710 + -1.1 2,057,742 - -0.3 2,057,824 - +0.7 2,057,919 - rpaC Synpcc7942_1987 0.32 +0.5 2,057,924 + rpaC Synpcc7942_1987 0.33 -0.3 2,057,932 - rpaC Synpcc7942_1987 0.36 -0.1 2,057,938 - rpaC Synpcc7942_1987 0.38 +0.5 2,057,940 + rpaC Synpcc7942_1987 0.38 +1.0 2,057,948 - rpaC Synpcc7942_1987 0.41 +2.9 2,057,948 - rpaC Synpcc7942_1987 0.41 +1.3 2,057,976 + rpaC Synpcc7942_1987 0.50 +2.1 2,058,039 + rpaC Synpcc7942_1987 0.70 +0.3 2,058,044 - rpaC Synpcc7942_1987 0.71 -1.3 2,058,172 + +1.0 2,058,179 - -1.9 2,058,200 - +0.4 2,058,247 + +1.5 2,058,277 + +0.3 2,058,304 - +1.3 2,058,368 + -2.3 2,058,403 + -0.1 2,058,420 - +1.3 2,058,422 + +1.0 2,058,456 - -0.6 2,058,472 - +3.1 2,058,472 - -2.9 2,058,479 - +2.5 2,058,486 - +0.0 2,058,488 + +1.7 2,058,496 + -2.3 2,058,501 + +0.4 2,058,504 - -3.3 2,058,518 + +2.3 2,058,610 - +1.3 2,058,638 + +1.9 2,058,644 - +1.7
Or see this region's nucleotide sequence