Experiment: Polymyxin B sulfate 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0816 and Synpcc7942_0817 are separated by 104 nucleotides Synpcc7942_0817 and purS are separated by 231 nucleotides purS and purL overlap by 4 nucleotides
Synpcc7942_0816: Synpcc7942_0816 - diguanylate cyclase/phosphodiesterase, at 810,181 to 812,226
_0816
Synpcc7942_0817: Synpcc7942_0817 - putative ferric uptake regulator, FUR family, at 812,331 to 812,723
_0817
Synpcc7942_0818: purS - phosphoribosylformylglycinamidine synthase subunit PurS, at 812,955 to 813,224
purS
Synpcc7942_0819: purL - phosphoribosylformylglycinamidine synthase subunit I, at 813,221 to 813,886
purL
Position (kb)
812
813 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 811.361 kb on + strand, within Synpcc7942_0816 at 811.387 kb on - strand, within Synpcc7942_0816 at 811.397 kb on + strand at 811.421 kb on - strand at 811.440 kb on - strand, within Synpcc7942_0816 at 811.445 kb on - strand, within Synpcc7942_0816 at 811.622 kb on + strand, within Synpcc7942_0816 at 811.644 kb on - strand, within Synpcc7942_0816 at 811.650 kb on - strand, within Synpcc7942_0816 at 811.670 kb on + strand, within Synpcc7942_0816 at 811.702 kb on - strand, within Synpcc7942_0816 at 811.702 kb on - strand, within Synpcc7942_0816 at 811.702 kb on - strand, within Synpcc7942_0816 at 811.758 kb on - strand, within Synpcc7942_0816 at 811.865 kb on + strand, within Synpcc7942_0816 at 811.871 kb on + strand, within Synpcc7942_0816 at 811.873 kb on - strand, within Synpcc7942_0816 at 812.156 kb on + strand at 812.217 kb on - strand at 812.227 kb on + strand at 812.375 kb on + strand, within Synpcc7942_0817 at 812.383 kb on - strand, within Synpcc7942_0817 at 812.409 kb on + strand, within Synpcc7942_0817 at 812.420 kb on + strand, within Synpcc7942_0817 at 812.433 kb on + strand, within Synpcc7942_0817 at 812.433 kb on + strand, within Synpcc7942_0817 at 812.438 kb on + strand, within Synpcc7942_0817 at 812.441 kb on - strand, within Synpcc7942_0817 at 812.441 kb on - strand, within Synpcc7942_0817 at 812.473 kb on - strand, within Synpcc7942_0817 at 812.475 kb on - strand, within Synpcc7942_0817 at 812.508 kb on + strand, within Synpcc7942_0817 at 812.515 kb on + strand, within Synpcc7942_0817 at 812.515 kb on + strand, within Synpcc7942_0817 at 812.515 kb on + strand, within Synpcc7942_0817 at 812.523 kb on - strand, within Synpcc7942_0817 at 812.523 kb on - strand, within Synpcc7942_0817 at 812.523 kb on - strand, within Synpcc7942_0817 at 812.528 kb on - strand, within Synpcc7942_0817 at 812.558 kb on + strand, within Synpcc7942_0817 at 812.566 kb on - strand, within Synpcc7942_0817 at 812.568 kb on + strand, within Synpcc7942_0817 at 812.594 kb on - strand, within Synpcc7942_0817 at 812.605 kb on + strand, within Synpcc7942_0817 at 812.621 kb on - strand, within Synpcc7942_0817 at 812.621 kb on - strand, within Synpcc7942_0817 at 812.621 kb on - strand, within Synpcc7942_0817 at 812.638 kb on - strand, within Synpcc7942_0817 at 812.643 kb on - strand, within Synpcc7942_0817 at 812.645 kb on - strand, within Synpcc7942_0817 at 812.651 kb on - strand, within Synpcc7942_0817 at 812.661 kb on - strand, within Synpcc7942_0817 at 812.663 kb on + strand, within Synpcc7942_0817 at 812.673 kb on + strand, within Synpcc7942_0817 at 812.673 kb on + strand, within Synpcc7942_0817 at 812.681 kb on - strand, within Synpcc7942_0817 at 812.718 kb on + strand at 812.718 kb on + strand at 812.718 kb on + strand at 812.725 kb on + strand at 812.727 kb on - strand at 812.731 kb on + strand at 812.744 kb on + strand at 812.745 kb on + strand at 812.745 kb on + strand at 812.759 kb on - strand at 812.774 kb on - strand at 812.778 kb on + strand at 812.779 kb on + strand at 812.793 kb on - strand at 812.793 kb on - strand at 812.838 kb on + strand at 812.846 kb on + strand at 812.851 kb on + strand at 812.854 kb on - strand at 812.873 kb on - strand at 812.893 kb on + strand at 812.904 kb on - strand at 813.219 kb on - strand at 813.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.2 mg/ml remove 811,361 + Synpcc7942_0816 0.58 +1.2 811,387 - Synpcc7942_0816 0.59 +1.0 811,397 + +0.5 811,421 - -2.9 811,440 - Synpcc7942_0816 0.62 +0.7 811,445 - Synpcc7942_0816 0.62 -3.7 811,622 + Synpcc7942_0816 0.70 +1.5 811,644 - Synpcc7942_0816 0.72 +0.1 811,650 - Synpcc7942_0816 0.72 +0.7 811,670 + Synpcc7942_0816 0.73 -0.9 811,702 - Synpcc7942_0816 0.74 -1.3 811,702 - Synpcc7942_0816 0.74 -0.7 811,702 - Synpcc7942_0816 0.74 -0.0 811,758 - Synpcc7942_0816 0.77 +1.3 811,865 + Synpcc7942_0816 0.82 -2.3 811,871 + Synpcc7942_0816 0.83 +3.0 811,873 - Synpcc7942_0816 0.83 +1.3 812,156 + -1.3 812,217 - +0.8 812,227 + -0.0 812,375 + Synpcc7942_0817 0.11 -2.3 812,383 - Synpcc7942_0817 0.13 -0.3 812,409 + Synpcc7942_0817 0.20 -1.1 812,420 + Synpcc7942_0817 0.23 -1.1 812,433 + Synpcc7942_0817 0.26 -1.3 812,433 + Synpcc7942_0817 0.26 -1.8 812,438 + Synpcc7942_0817 0.27 -1.7 812,441 - Synpcc7942_0817 0.28 -0.7 812,441 - Synpcc7942_0817 0.28 -0.1 812,473 - Synpcc7942_0817 0.36 -3.7 812,475 - Synpcc7942_0817 0.37 -0.1 812,508 + Synpcc7942_0817 0.45 -3.3 812,515 + Synpcc7942_0817 0.47 -4.1 812,515 + Synpcc7942_0817 0.47 -2.3 812,515 + Synpcc7942_0817 0.47 -0.0 812,523 - Synpcc7942_0817 0.49 -2.3 812,523 - Synpcc7942_0817 0.49 -2.6 812,523 - Synpcc7942_0817 0.49 -0.7 812,528 - Synpcc7942_0817 0.50 -1.3 812,558 + Synpcc7942_0817 0.58 +2.3 812,566 - Synpcc7942_0817 0.60 -1.1 812,568 + Synpcc7942_0817 0.60 -2.3 812,594 - Synpcc7942_0817 0.67 -2.7 812,605 + Synpcc7942_0817 0.70 -2.5 812,621 - Synpcc7942_0817 0.74 -1.3 812,621 - Synpcc7942_0817 0.74 -2.7 812,621 - Synpcc7942_0817 0.74 -2.9 812,638 - Synpcc7942_0817 0.78 +2.0 812,643 - Synpcc7942_0817 0.79 -3.3 812,645 - Synpcc7942_0817 0.80 -1.7 812,651 - Synpcc7942_0817 0.81 -1.7 812,661 - Synpcc7942_0817 0.84 -1.3 812,663 + Synpcc7942_0817 0.84 -2.1 812,673 + Synpcc7942_0817 0.87 -2.3 812,673 + Synpcc7942_0817 0.87 -3.3 812,681 - Synpcc7942_0817 0.89 -0.9 812,718 + -0.0 812,718 + +0.5 812,718 + +0.8 812,725 + +0.7 812,727 - +1.1 812,731 + -0.0 812,744 + -2.3 812,745 + -1.3 812,745 + -1.3 812,759 - -3.3 812,774 - -2.3 812,778 + +0.8 812,779 + -2.4 812,793 - -1.3 812,793 - +0.4 812,838 + +1.2 812,846 + +0.7 812,851 + +0.6 812,854 - +3.2 812,873 - -1.9 812,893 + +0.1 812,904 - +0.7 813,219 - +1.3 813,219 - +0.3
Or see this region's nucleotide sequence