Experiment: Polymyxin B sulfate 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyA and Synpcc7942_0283 are separated by 196 nucleotides Synpcc7942_0283 and ccmK3 are separated by 77 nucleotides ccmK3 and ccmK4 are separated by 44 nucleotides ccmK4 and Synpcc7942_0286 are separated by 84 nucleotides
Synpcc7942_0282: glyA - serine hydroxymethyltransferase, at 278,139 to 279,422
glyA
Synpcc7942_0283: Synpcc7942_0283 - Dihydrouridine synthase TIM-barrel protein nifR3, at 279,619 to 280,623
_0283
Synpcc7942_0284: ccmK3 - carbon dioxide concentrating mechanism protein CcmK, at 280,701 to 281,009
ccmK3
Synpcc7942_0285: ccmK4 - carbon dioxide concentrating mechanism protein CcmK, at 281,054 to 281,395
ccmK4
Synpcc7942_0286: Synpcc7942_0286 - hypothetical protein, at 281,480 to 281,842
_0286
Position (kb)
279
280
281 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 279.441 kb on + strand at 279.534 kb on + strand at 279.534 kb on + strand at 279.591 kb on + strand at 279.599 kb on - strand at 279.599 kb on - strand at 279.599 kb on - strand at 279.615 kb on + strand at 279.615 kb on + strand at 279.623 kb on - strand at 279.627 kb on + strand at 279.635 kb on - strand at 279.635 kb on - strand at 279.635 kb on - strand at 279.635 kb on - strand at 279.635 kb on - strand at 279.650 kb on + strand at 279.662 kb on + strand at 279.668 kb on - strand at 279.672 kb on + strand at 279.688 kb on - strand at 279.702 kb on + strand at 279.702 kb on + strand at 279.702 kb on + strand at 279.710 kb on - strand at 279.710 kb on - strand at 279.710 kb on - strand at 279.710 kb on - strand at 279.736 kb on + strand, within Synpcc7942_0283 at 279.736 kb on + strand, within Synpcc7942_0283 at 279.738 kb on - strand, within Synpcc7942_0283 at 279.754 kb on + strand, within Synpcc7942_0283 at 279.773 kb on + strand, within Synpcc7942_0283 at 279.781 kb on - strand, within Synpcc7942_0283 at 279.782 kb on + strand, within Synpcc7942_0283 at 279.790 kb on - strand, within Synpcc7942_0283 at 279.790 kb on - strand, within Synpcc7942_0283 at 279.803 kb on + strand, within Synpcc7942_0283 at 279.812 kb on + strand, within Synpcc7942_0283 at 279.831 kb on + strand, within Synpcc7942_0283 at 279.839 kb on - strand, within Synpcc7942_0283 at 279.868 kb on + strand, within Synpcc7942_0283 at 279.893 kb on + strand, within Synpcc7942_0283 at 279.927 kb on - strand, within Synpcc7942_0283 at 279.950 kb on - strand, within Synpcc7942_0283 at 279.971 kb on - strand, within Synpcc7942_0283 at 279.976 kb on + strand, within Synpcc7942_0283 at 280.013 kb on + strand, within Synpcc7942_0283 at 280.021 kb on - strand, within Synpcc7942_0283 at 280.023 kb on + strand, within Synpcc7942_0283 at 280.023 kb on + strand, within Synpcc7942_0283 at 280.023 kb on + strand, within Synpcc7942_0283 at 280.028 kb on + strand, within Synpcc7942_0283 at 280.031 kb on - strand, within Synpcc7942_0283 at 280.041 kb on - strand, within Synpcc7942_0283 at 280.043 kb on + strand, within Synpcc7942_0283 at 280.052 kb on + strand, within Synpcc7942_0283 at 280.052 kb on + strand, within Synpcc7942_0283 at 280.052 kb on + strand, within Synpcc7942_0283 at 280.060 kb on - strand, within Synpcc7942_0283 at 280.064 kb on - strand, within Synpcc7942_0283 at 280.075 kb on + strand, within Synpcc7942_0283 at 280.075 kb on + strand, within Synpcc7942_0283 at 280.075 kb on + strand, within Synpcc7942_0283 at 280.077 kb on + strand at 280.092 kb on + strand, within Synpcc7942_0283 at 280.114 kb on - strand, within Synpcc7942_0283 at 280.116 kb on + strand, within Synpcc7942_0283 at 280.125 kb on + strand, within Synpcc7942_0283 at 280.134 kb on - strand, within Synpcc7942_0283 at 280.134 kb on - strand, within Synpcc7942_0283 at 280.134 kb on - strand, within Synpcc7942_0283 at 280.159 kb on + strand, within Synpcc7942_0283 at 280.159 kb on + strand, within Synpcc7942_0283 at 280.168 kb on - strand, within Synpcc7942_0283 at 280.205 kb on - strand, within Synpcc7942_0283 at 280.216 kb on + strand, within Synpcc7942_0283 at 280.216 kb on + strand, within Synpcc7942_0283 at 280.229 kb on - strand, within Synpcc7942_0283 at 280.266 kb on + strand, within Synpcc7942_0283 at 280.271 kb on - strand, within Synpcc7942_0283 at 280.276 kb on - strand, within Synpcc7942_0283 at 280.283 kb on + strand, within Synpcc7942_0283 at 280.283 kb on + strand, within Synpcc7942_0283 at 280.291 kb on - strand, within Synpcc7942_0283 at 280.295 kb on - strand, within Synpcc7942_0283 at 280.296 kb on - strand, within Synpcc7942_0283 at 280.296 kb on - strand, within Synpcc7942_0283 at 280.342 kb on - strand, within Synpcc7942_0283 at 280.363 kb on + strand, within Synpcc7942_0283 at 280.473 kb on - strand, within Synpcc7942_0283 at 280.541 kb on - strand at 280.541 kb on - strand at 280.556 kb on - strand at 280.566 kb on + strand at 280.576 kb on + strand at 280.600 kb on + strand at 280.968 kb on - strand, within ccmK3 at 281.396 kb on + strand at 281.396 kb on + strand at 281.449 kb on + strand at 281.506 kb on + strand at 281.567 kb on + strand, within Synpcc7942_0286 at 281.567 kb on + strand, within Synpcc7942_0286
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.2 mg/ml remove 279,441 + -5.6 279,534 + -2.3 279,534 + -4.1 279,591 + +0.3 279,599 - +1.5 279,599 - +1.9 279,599 - +0.5 279,615 + +0.3 279,615 + +0.6 279,623 - -1.3 279,627 + -0.1 279,635 - +0.3 279,635 - -1.3 279,635 - +1.0 279,635 - -1.3 279,635 - -0.5 279,650 + +0.4 279,662 + +2.4 279,668 - +0.1 279,672 + -1.3 279,688 - -2.3 279,702 + -1.6 279,702 + +0.3 279,702 + -0.3 279,710 - -0.3 279,710 - +0.9 279,710 - -0.2 279,710 - -0.3 279,736 + Synpcc7942_0283 0.12 +1.1 279,736 + Synpcc7942_0283 0.12 +1.0 279,738 - Synpcc7942_0283 0.12 +2.4 279,754 + Synpcc7942_0283 0.13 +0.3 279,773 + Synpcc7942_0283 0.15 -0.7 279,781 - Synpcc7942_0283 0.16 +0.4 279,782 + Synpcc7942_0283 0.16 +0.1 279,790 - Synpcc7942_0283 0.17 +2.3 279,790 - Synpcc7942_0283 0.17 -0.0 279,803 + Synpcc7942_0283 0.18 +0.7 279,812 + Synpcc7942_0283 0.19 +2.2 279,831 + Synpcc7942_0283 0.21 +1.5 279,839 - Synpcc7942_0283 0.22 +1.2 279,868 + Synpcc7942_0283 0.25 -1.6 279,893 + Synpcc7942_0283 0.27 +0.7 279,927 - Synpcc7942_0283 0.31 -0.3 279,950 - Synpcc7942_0283 0.33 +1.9 279,971 - Synpcc7942_0283 0.35 +1.0 279,976 + Synpcc7942_0283 0.36 +3.7 280,013 + Synpcc7942_0283 0.39 +2.0 280,021 - Synpcc7942_0283 0.40 +1.9 280,023 + Synpcc7942_0283 0.40 -0.5 280,023 + Synpcc7942_0283 0.40 -1.3 280,023 + Synpcc7942_0283 0.40 +1.1 280,028 + Synpcc7942_0283 0.41 +1.1 280,031 - Synpcc7942_0283 0.41 -1.1 280,041 - Synpcc7942_0283 0.42 -2.3 280,043 + Synpcc7942_0283 0.42 +0.8 280,052 + Synpcc7942_0283 0.43 -0.7 280,052 + Synpcc7942_0283 0.43 +2.6 280,052 + Synpcc7942_0283 0.43 +0.1 280,060 - Synpcc7942_0283 0.44 -0.3 280,064 - Synpcc7942_0283 0.44 +1.1 280,075 + Synpcc7942_0283 0.45 -2.9 280,075 + Synpcc7942_0283 0.45 +1.1 280,075 + Synpcc7942_0283 0.45 +2.7 280,077 + +1.3 280,092 + Synpcc7942_0283 0.47 -0.3 280,114 - Synpcc7942_0283 0.49 +2.1 280,116 + Synpcc7942_0283 0.49 -2.0 280,125 + Synpcc7942_0283 0.50 +1.5 280,134 - Synpcc7942_0283 0.51 +0.3 280,134 - Synpcc7942_0283 0.51 +0.5 280,134 - Synpcc7942_0283 0.51 +0.4 280,159 + Synpcc7942_0283 0.54 -0.9 280,159 + Synpcc7942_0283 0.54 +0.1 280,168 - Synpcc7942_0283 0.55 +0.3 280,205 - Synpcc7942_0283 0.58 -2.3 280,216 + Synpcc7942_0283 0.59 -1.3 280,216 + Synpcc7942_0283 0.59 +0.8 280,229 - Synpcc7942_0283 0.61 +1.3 280,266 + Synpcc7942_0283 0.64 +1.6 280,271 - Synpcc7942_0283 0.65 -1.9 280,276 - Synpcc7942_0283 0.65 -2.3 280,283 + Synpcc7942_0283 0.66 -1.0 280,283 + Synpcc7942_0283 0.66 +1.6 280,291 - Synpcc7942_0283 0.67 +0.1 280,295 - Synpcc7942_0283 0.67 +0.5 280,296 - Synpcc7942_0283 0.67 +2.1 280,296 - Synpcc7942_0283 0.67 -1.0 280,342 - Synpcc7942_0283 0.72 -0.3 280,363 + Synpcc7942_0283 0.74 +1.4 280,473 - Synpcc7942_0283 0.85 +1.0 280,541 - +0.5 280,541 - +1.1 280,556 - +1.9 280,566 + -1.0 280,576 + +1.7 280,600 + +1.3 280,968 - ccmK3 Synpcc7942_0284 0.86 -2.0 281,396 + -2.6 281,396 + +1.2 281,449 + -1.3 281,506 + +0.5 281,567 + Synpcc7942_0286 0.24 -0.5 281,567 + Synpcc7942_0286 0.24 +1.8
Or see this region's nucleotide sequence