Experiment: copper (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1211 and SMa1213 are separated by 228 nucleotides SMa1213 and SMa1214 are separated by 3 nucleotides SMa1214 and SMa1216 are separated by 12 nucleotides SMa1216 and SMa1220 are separated by 13 nucleotides
SMa1211: SMa1211 - FixG iron sulfur membrane protein, at 665,557 to 667,131
SMa1211
SMa1213: SMa1213 - FixP1 di-heme cytochrome c, at 667,360 to 668,229
SMa1213
SMa1214: SMa1214 - FixQ1 nitrogen fixation protein, at 668,233 to 668,385
SMa1214
SMa1216: SMa1216 - cbb3-type cytochrome c oxidase subunit II, at 668,398 to 669,129
SMa1216
SMa1220: SMa1220 - cbb3-type cytochrome c oxidase subunit I, at 669,143 to 670,762
SMa1220
Position (kb) 667
668
669 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 666.369 kb on - strand, within SMa1211 at 666.394 kb on + strand at 666.394 kb on + strand, within SMa1211 at 666.395 kb on - strand, within SMa1211 at 666.429 kb on + strand, within SMa1211 at 666.429 kb on + strand, within SMa1211 at 666.429 kb on + strand, within SMa1211 at 666.429 kb on + strand, within SMa1211 at 666.429 kb on + strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.431 kb on + strand, within SMa1211 at 666.431 kb on + strand, within SMa1211 at 666.431 kb on + strand, within SMa1211 at 666.431 kb on + strand, within SMa1211 at 666.431 kb on + strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.470 kb on + strand, within SMa1211 at 666.471 kb on - strand, within SMa1211 at 666.533 kb on - strand, within SMa1211 at 666.566 kb on + strand, within SMa1211 at 666.567 kb on - strand, within SMa1211 at 666.567 kb on - strand at 666.567 kb on - strand, within SMa1211 at 666.637 kb on + strand, within SMa1211 at 666.870 kb on - strand, within SMa1211 at 666.953 kb on + strand, within SMa1211 at 666.953 kb on + strand, within SMa1211 at 666.956 kb on + strand, within SMa1211 at 667.151 kb on - strand at 667.361 kb on + strand at 667.653 kb on - strand, within SMa1213 at 667.653 kb on - strand, within SMa1213 at 667.758 kb on + strand, within SMa1213 at 667.767 kb on - strand, within SMa1213 at 667.767 kb on - strand, within SMa1213 at 667.767 kb on - strand, within SMa1213 at 668.085 kb on - strand, within SMa1213 at 668.112 kb on - strand at 668.120 kb on + strand, within SMa1213 at 668.146 kb on - strand at 668.375 kb on + strand at 668.376 kb on - strand at 668.376 kb on - strand at 668.423 kb on + strand at 668.621 kb on + strand, within SMa1216 at 668.621 kb on + strand, within SMa1216 at 668.702 kb on + strand, within SMa1216 at 668.703 kb on - strand, within SMa1216 at 668.703 kb on - strand, within SMa1216 at 668.774 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.841 kb on - strand, within SMa1216 at 668.865 kb on - strand, within SMa1216 at 668.924 kb on + strand, within SMa1216 at 668.925 kb on - strand, within SMa1216 at 668.925 kb on - strand, within SMa1216 at 668.925 kb on - strand, within SMa1216 at 668.942 kb on + strand, within SMa1216 at 668.942 kb on + strand, within SMa1216 at 668.943 kb on - strand, within SMa1216 at 668.943 kb on - strand, within SMa1216 at 668.975 kb on + strand, within SMa1216 at 668.975 kb on + strand, within SMa1216 at 668.975 kb on + strand, within SMa1216 at 668.976 kb on - strand, within SMa1216 at 669.017 kb on + strand, within SMa1216 at 669.073 kb on + strand at 669.074 kb on - strand at 669.135 kb on - strand at 669.135 kb on - strand at 669.135 kb on - strand at 669.144 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 1 mM remove 666,369 - SMa1211 0.52 +1.3 666,394 + +1.0 666,394 + SMa1211 0.53 -0.6 666,395 - SMa1211 0.53 +1.0 666,429 + SMa1211 0.55 +1.9 666,429 + SMa1211 0.55 -1.8 666,429 + SMa1211 0.55 -0.0 666,429 + SMa1211 0.55 -0.8 666,429 + SMa1211 0.55 +1.0 666,430 - SMa1211 0.55 -0.9 666,430 - SMa1211 0.55 -0.6 666,430 - SMa1211 0.55 -0.3 666,430 - SMa1211 0.55 -0.3 666,430 - SMa1211 0.55 -0.5 666,430 - SMa1211 0.55 +0.4 666,431 + SMa1211 0.55 +0.4 666,431 + SMa1211 0.55 +0.2 666,431 + SMa1211 0.55 -3.6 666,431 + SMa1211 0.55 -0.6 666,431 + SMa1211 0.55 +1.0 666,432 - SMa1211 0.56 +0.2 666,432 - SMa1211 0.56 +0.9 666,432 - SMa1211 0.56 +0.9 666,432 - SMa1211 0.56 -2.0 666,432 - SMa1211 0.56 +1.0 666,432 - SMa1211 0.56 -1.7 666,470 + SMa1211 0.58 +0.1 666,471 - SMa1211 0.58 -0.5 666,533 - SMa1211 0.62 +0.7 666,566 + SMa1211 0.64 -0.1 666,567 - SMa1211 0.64 -3.8 666,567 - +4.0 666,567 - SMa1211 0.64 +1.7 666,637 + SMa1211 0.69 -0.0 666,870 - SMa1211 0.83 -2.0 666,953 + SMa1211 0.89 +0.8 666,953 + SMa1211 0.89 -0.0 666,956 + SMa1211 0.89 -0.2 667,151 - -1.0 667,361 + -1.4 667,653 - SMa1213 0.34 +0.4 667,653 - SMa1213 0.34 -0.6 667,758 + SMa1213 0.46 -1.8 667,767 - SMa1213 0.47 +0.5 667,767 - SMa1213 0.47 -1.8 667,767 - SMa1213 0.47 -0.4 668,085 - SMa1213 0.83 +0.8 668,112 - +2.8 668,120 + SMa1213 0.87 -3.1 668,146 - +0.3 668,375 + +0.2 668,376 - +0.1 668,376 - -0.2 668,423 + -3.5 668,621 + SMa1216 0.30 -1.3 668,621 + SMa1216 0.30 +0.1 668,702 + SMa1216 0.42 +0.0 668,703 - SMa1216 0.42 -3.4 668,703 - SMa1216 0.42 +0.1 668,774 + SMa1216 0.51 -3.5 668,840 + SMa1216 0.60 +0.3 668,840 + SMa1216 0.60 -0.5 668,840 + SMa1216 0.60 -2.8 668,840 + SMa1216 0.60 +1.1 668,841 - SMa1216 0.61 -0.0 668,865 - SMa1216 0.64 -0.7 668,924 + SMa1216 0.72 +1.3 668,925 - SMa1216 0.72 -0.5 668,925 - SMa1216 0.72 +1.0 668,925 - SMa1216 0.72 -0.6 668,942 + SMa1216 0.74 -1.9 668,942 + SMa1216 0.74 -2.6 668,943 - SMa1216 0.74 -0.9 668,943 - SMa1216 0.74 -0.7 668,975 + SMa1216 0.79 +0.6 668,975 + SMa1216 0.79 +0.2 668,975 + SMa1216 0.79 +0.5 668,976 - SMa1216 0.79 +1.4 669,017 + SMa1216 0.85 -0.9 669,073 + -0.6 669,074 - -1.6 669,135 - +1.0 669,135 - +0.4 669,135 - +0.4 669,144 + -1.7
Or see this region's nucleotide sequence