Experiment: copper (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1207 and SMa1208 are separated by 73 nucleotides SMa1208 and SMa1209 overlap by 4 nucleotides SMa1209 and SMa1210 overlap by 4 nucleotides SMa1210 and SMa1211 overlap by 4 nucleotides
SMa1207: SMa1207 - Crp family transcriptional regulator, at 661,869 to 662,549
SMa1207
SMa1208: SMa1208 - FixS1 nitrogen fixation protein, at 662,623 to 662,790
SMa1208
SMa1209: SMa1209 - FixI1 ATPase, at 662,787 to 665,060
SMa1209
SMa1210: SMa1210 - nitrogen fixation protein FixH, at 665,057 to 665,560
SMa1210
SMa1211: SMa1211 - FixG iron sulfur membrane protein, at 665,557 to 667,131
SMa1211
Position (kb)
662
663
664
665
666 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 661.789 kb on + strand at 661.859 kb on + strand at 661.859 kb on + strand at 661.859 kb on + strand at 661.860 kb on - strand at 661.860 kb on - strand at 662.068 kb on + strand, within SMa1207 at 662.133 kb on - strand, within SMa1207 at 662.272 kb on + strand, within SMa1207 at 662.272 kb on + strand, within SMa1207 at 662.272 kb on + strand, within SMa1207 at 662.273 kb on - strand, within SMa1207 at 662.273 kb on - strand, within SMa1207 at 662.273 kb on - strand, within SMa1207 at 662.273 kb on - strand, within SMa1207 at 662.431 kb on + strand, within SMa1207 at 662.431 kb on + strand, within SMa1207 at 662.432 kb on - strand, within SMa1207 at 662.587 kb on + strand at 662.587 kb on + strand at 662.588 kb on - strand at 662.588 kb on - strand at 662.620 kb on + strand at 662.621 kb on - strand at 662.621 kb on - strand at 662.621 kb on - strand at 662.621 kb on - strand at 662.624 kb on + strand at 662.669 kb on - strand, within SMa1208 at 662.774 kb on + strand at 662.774 kb on + strand at 662.774 kb on + strand at 662.774 kb on + strand at 662.775 kb on - strand at 662.778 kb on + strand at 662.778 kb on + strand at 662.778 kb on + strand at 662.778 kb on + strand at 662.779 kb on - strand at 662.779 kb on - strand at 662.779 kb on - strand at 662.779 kb on - strand at 662.811 kb on + strand at 662.811 kb on + strand at 662.969 kb on - strand at 662.999 kb on + strand at 663.207 kb on + strand, within SMa1209 at 663.582 kb on + strand, within SMa1209 at 663.582 kb on + strand, within SMa1209 at 663.583 kb on - strand, within SMa1209 at 663.614 kb on + strand, within SMa1209 at 663.614 kb on + strand, within SMa1209 at 663.889 kb on - strand, within SMa1209 at 664.016 kb on + strand, within SMa1209 at 664.146 kb on + strand, within SMa1209 at 664.239 kb on + strand, within SMa1209 at 664.351 kb on + strand, within SMa1209 at 664.456 kb on + strand, within SMa1209 at 664.534 kb on + strand, within SMa1209 at 664.552 kb on + strand, within SMa1209 at 664.552 kb on + strand, within SMa1209 at 664.553 kb on - strand, within SMa1209 at 664.794 kb on - strand, within SMa1209 at 664.794 kb on - strand, within SMa1209 at 664.921 kb on + strand at 664.938 kb on + strand at 664.939 kb on - strand at 665.042 kb on + strand at 665.042 kb on + strand at 665.400 kb on + strand, within SMa1210 at 665.400 kb on + strand, within SMa1210 at 665.401 kb on - strand at 665.401 kb on - strand, within SMa1210 at 665.806 kb on - strand, within SMa1211 at 665.806 kb on - strand, within SMa1211 at 665.849 kb on + strand, within SMa1211 at 665.849 kb on + strand, within SMa1211 at 665.849 kb on + strand, within SMa1211 at 665.850 kb on - strand, within SMa1211
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 1 mM remove 661,789 + +0.5 661,859 + -0.1 661,859 + -0.5 661,859 + -1.6 661,860 - +0.1 661,860 - +0.9 662,068 + SMa1207 0.29 +0.5 662,133 - SMa1207 0.39 -0.4 662,272 + SMa1207 0.59 -3.3 662,272 + SMa1207 0.59 +0.7 662,272 + SMa1207 0.59 +0.5 662,273 - SMa1207 0.59 -3.4 662,273 - SMa1207 0.59 -0.4 662,273 - SMa1207 0.59 +1.3 662,273 - SMa1207 0.59 +0.3 662,431 + SMa1207 0.83 -0.4 662,431 + SMa1207 0.83 -0.0 662,432 - SMa1207 0.83 -0.6 662,587 + +1.8 662,587 + +0.9 662,588 - +1.0 662,588 - +1.3 662,620 + -0.2 662,621 - -1.5 662,621 - -0.0 662,621 - -2.2 662,621 - -3.0 662,624 + -3.2 662,669 - SMa1208 0.27 -1.4 662,774 + -1.9 662,774 + -1.2 662,774 + -1.5 662,774 + -0.1 662,775 - +0.8 662,778 + +1.0 662,778 + -2.7 662,778 + -0.6 662,778 + -0.0 662,779 - -2.5 662,779 - -1.4 662,779 - +0.3 662,779 - -3.1 662,811 + +1.5 662,811 + +2.0 662,969 - -1.4 662,999 + -0.8 663,207 + SMa1209 0.18 -2.4 663,582 + SMa1209 0.35 -2.5 663,582 + SMa1209 0.35 -0.8 663,583 - SMa1209 0.35 +0.1 663,614 + SMa1209 0.36 +1.2 663,614 + SMa1209 0.36 +1.3 663,889 - SMa1209 0.48 -1.6 664,016 + SMa1209 0.54 +0.2 664,146 + SMa1209 0.60 -0.9 664,239 + SMa1209 0.64 +0.0 664,351 + SMa1209 0.69 -0.3 664,456 + SMa1209 0.73 +0.6 664,534 + SMa1209 0.77 +0.3 664,552 + SMa1209 0.78 +2.4 664,552 + SMa1209 0.78 -0.1 664,553 - SMa1209 0.78 -2.1 664,794 - SMa1209 0.88 -1.1 664,794 - SMa1209 0.88 -0.5 664,921 + -1.6 664,938 + +1.4 664,939 - +0.1 665,042 + +3.0 665,042 + +0.7 665,400 + SMa1210 0.68 -0.7 665,400 + SMa1210 0.68 -0.7 665,401 - +2.2 665,401 - SMa1210 0.68 -1.8 665,806 - SMa1211 0.16 -2.6 665,806 - SMa1211 0.16 -1.9 665,849 + SMa1211 0.19 -2.7 665,849 + SMa1211 0.19 -0.8 665,849 + SMa1211 0.19 +0.5 665,850 - SMa1211 0.19 +0.7
Or see this region's nucleotide sequence