Experiment: R2A 23C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22985 and RR42_RS22990 are separated by 320 nucleotides RR42_RS22990 and RR42_RS22995 are separated by 113 nucleotides RR42_RS22995 and RR42_RS23000 are separated by 189 nucleotides
RR42_RS22985: RR42_RS22985 - 16S ribosomal RNA, at 489,212 to 490,785
_RS22985
RR42_RS22990: RR42_RS22990 - 23S ribosomal RNA, at 491,106 to 494,102
_RS22990
RR42_RS22995: RR42_RS22995 - 5S ribosomal RNA, at 494,216 to 494,328
_RS22995
RR42_RS23000: RR42_RS23000 - acylaldehyde oxidase, at 494,518 to 496,803
_RS23000
Position (kb)
491
492
493
494
495 Strain fitness (log2 ratio)
-1
0
1 at 490.798 kb on + strand at 492.248 kb on + strand, within RR42_RS22990 at 492.248 kb on + strand, within RR42_RS22990 at 492.286 kb on + strand, within RR42_RS22990 at 492.365 kb on + strand, within RR42_RS22990 at 494.357 kb on + strand at 494.714 kb on + strand at 494.715 kb on - strand at 494.924 kb on + strand, within RR42_RS23000 at 494.924 kb on + strand, within RR42_RS23000 at 494.925 kb on - strand, within RR42_RS23000 at 494.925 kb on - strand, within RR42_RS23000 at 494.925 kb on - strand, within RR42_RS23000 at 494.939 kb on + strand, within RR42_RS23000 at 494.939 kb on + strand, within RR42_RS23000 at 494.940 kb on - strand, within RR42_RS23000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A 23C remove 490,798 + +1.0 492,248 + RR42_RS22990 0.38 -0.7 492,248 + RR42_RS22990 0.38 -1.4 492,286 + RR42_RS22990 0.39 -0.0 492,365 + RR42_RS22990 0.42 -0.8 494,357 + -0.6 494,714 + +0.1 494,715 - +0.2 494,924 + RR42_RS23000 0.18 +0.6 494,924 + RR42_RS23000 0.18 -0.3 494,925 - RR42_RS23000 0.18 +0.0 494,925 - RR42_RS23000 0.18 +1.1 494,925 - RR42_RS23000 0.18 +0.3 494,939 + RR42_RS23000 0.18 +0.4 494,939 + RR42_RS23000 0.18 -0.7 494,940 - RR42_RS23000 0.18 -1.2
Or see this region's nucleotide sequence