Experiment: R2A 23C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00290 and RR42_RS00295 are separated by 103 nucleotides RR42_RS00295 and RR42_RS00300 are separated by 64 nucleotides
RR42_RS00290: RR42_RS00290 - sialidase, at 83,329 to 84,483
_RS00290
RR42_RS00295: RR42_RS00295 - DEAD/DEAH box helicase, at 84,587 to 86,008
_RS00295
RR42_RS00300: RR42_RS00300 - kinase, at 86,073 to 86,921
_RS00300
Position (kb) 84
85
86
87 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 83.986 kb on + strand, within RR42_RS00290 at 83.986 kb on + strand, within RR42_RS00290 at 83.987 kb on - strand, within RR42_RS00290 at 83.987 kb on - strand, within RR42_RS00290 at 83.987 kb on - strand, within RR42_RS00290 at 84.322 kb on + strand, within RR42_RS00290 at 84.334 kb on + strand, within RR42_RS00290 at 84.334 kb on + strand, within RR42_RS00290 at 84.335 kb on - strand, within RR42_RS00290 at 84.463 kb on + strand at 84.463 kb on + strand at 84.464 kb on - strand at 84.505 kb on + strand at 84.506 kb on - strand at 84.576 kb on + strand at 84.997 kb on + strand, within RR42_RS00295 at 84.997 kb on + strand, within RR42_RS00295 at 84.998 kb on - strand, within RR42_RS00295 at 85.130 kb on + strand, within RR42_RS00295 at 85.130 kb on + strand, within RR42_RS00295 at 85.131 kb on - strand, within RR42_RS00295 at 85.131 kb on - strand, within RR42_RS00295 at 85.131 kb on - strand, within RR42_RS00295 at 85.598 kb on + strand, within RR42_RS00295 at 85.598 kb on + strand, within RR42_RS00295 at 85.598 kb on + strand, within RR42_RS00295 at 85.651 kb on + strand, within RR42_RS00295 at 85.691 kb on - strand, within RR42_RS00295 at 85.859 kb on + strand, within RR42_RS00295 at 85.907 kb on + strand at 85.907 kb on + strand at 85.907 kb on + strand at 85.908 kb on - strand at 86.166 kb on + strand, within RR42_RS00300 at 86.166 kb on + strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.167 kb on - strand, within RR42_RS00300 at 86.746 kb on - strand, within RR42_RS00300 at 86.769 kb on + strand, within RR42_RS00300 at 86.769 kb on + strand, within RR42_RS00300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A 23C remove 83,986 + RR42_RS00290 0.57 -1.5 83,986 + RR42_RS00290 0.57 +0.2 83,987 - RR42_RS00290 0.57 -1.0 83,987 - RR42_RS00290 0.57 -1.2 83,987 - RR42_RS00290 0.57 -0.0 84,322 + RR42_RS00290 0.86 -0.1 84,334 + RR42_RS00290 0.87 -0.2 84,334 + RR42_RS00290 0.87 -0.2 84,335 - RR42_RS00290 0.87 -0.3 84,463 + +0.4 84,463 + -0.0 84,464 - -0.9 84,505 + +0.5 84,506 - -1.1 84,576 + +1.6 84,997 + RR42_RS00295 0.29 -1.3 84,997 + RR42_RS00295 0.29 -0.0 84,998 - RR42_RS00295 0.29 -2.4 85,130 + RR42_RS00295 0.38 -0.6 85,130 + RR42_RS00295 0.38 -0.8 85,131 - RR42_RS00295 0.38 -1.3 85,131 - RR42_RS00295 0.38 -2.4 85,131 - RR42_RS00295 0.38 -0.7 85,598 + RR42_RS00295 0.71 -0.5 85,598 + RR42_RS00295 0.71 +0.3 85,598 + RR42_RS00295 0.71 -1.0 85,651 + RR42_RS00295 0.75 -0.3 85,691 - RR42_RS00295 0.78 -3.3 85,859 + RR42_RS00295 0.89 -1.9 85,907 + -1.4 85,907 + -1.2 85,907 + -0.6 85,908 - -0.9 86,166 + RR42_RS00300 0.11 -0.6 86,166 + RR42_RS00300 0.11 -0.0 86,167 - RR42_RS00300 0.11 +1.5 86,167 - RR42_RS00300 0.11 -0.5 86,167 - RR42_RS00300 0.11 -0.3 86,746 - RR42_RS00300 0.79 -0.6 86,769 + RR42_RS00300 0.82 -0.1 86,769 + RR42_RS00300 0.82 +1.0
Or see this region's nucleotide sequence