Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS04815

Experiment: formate McAA folic dropout

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS04805 and MMJJ_RS04810 are separated by 133 nucleotidesMMJJ_RS04810 and MMJJ_RS04815 are separated by 168 nucleotidesMMJJ_RS04815 and MMJJ_RS04820 are separated by 33 nucleotidesMMJJ_RS04820 and MMJJ_RS04825 are separated by 8 nucleotides MMJJ_RS04805: MMJJ_RS04805 - N-acetyl-gamma-glutamyl-phosphate reductase, at 900,427 to 901,458 _RS04805 MMJJ_RS04810: MMJJ_RS04810 - nitroreductase family protein, at 901,592 to 902,113 _RS04810 MMJJ_RS04815: MMJJ_RS04815 - TIM barrel protein, at 902,282 to 902,938 _RS04815 MMJJ_RS04820: MMJJ_RS04820 - hypothetical protein, at 902,972 to 903,781 _RS04820 MMJJ_RS04825: MMJJ_RS04825 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 903,790 to 905,070 _RS04825 Position (kb) 902 903Strain fitness (log2 ratio) -2 -1 0 1 2at 901.293 kb on - strand, within MMJJ_RS04805at 901.499 kb on + strandat 901.526 kb on - strandat 901.527 kb on + strandat 901.533 kb on - strandat 901.563 kb on + strandat 901.599 kb on - strandat 901.620 kb on - strandat 901.625 kb on - strandat 901.686 kb on + strand, within MMJJ_RS04810at 901.754 kb on - strand, within MMJJ_RS04810at 901.755 kb on + strand, within MMJJ_RS04810at 901.761 kb on - strand, within MMJJ_RS04810at 901.762 kb on + strand, within MMJJ_RS04810at 901.776 kb on + strand, within MMJJ_RS04810at 901.805 kb on - strand, within MMJJ_RS04810at 901.805 kb on - strandat 901.805 kb on - strand, within MMJJ_RS04810at 901.805 kb on - strand, within MMJJ_RS04810at 901.808 kb on - strand, within MMJJ_RS04810at 901.809 kb on + strand, within MMJJ_RS04810at 901.811 kb on - strand, within MMJJ_RS04810at 901.811 kb on - strand, within MMJJ_RS04810at 901.889 kb on - strand, within MMJJ_RS04810at 901.889 kb on - strand, within MMJJ_RS04810at 901.889 kb on - strand, within MMJJ_RS04810at 901.895 kb on - strand, within MMJJ_RS04810at 901.896 kb on + strand, within MMJJ_RS04810at 901.896 kb on + strand, within MMJJ_RS04810at 901.977 kb on + strand, within MMJJ_RS04810at 902.048 kb on - strand, within MMJJ_RS04810at 902.076 kb on - strandat 902.076 kb on - strandat 902.077 kb on + strandat 902.102 kb on - strandat 902.113 kb on - strandat 902.113 kb on - strandat 902.114 kb on + strandat 902.114 kb on + strandat 902.115 kb on - strandat 902.115 kb on - strandat 902.143 kb on + strandat 902.147 kb on + strandat 902.170 kb on - strandat 902.171 kb on + strandat 902.171 kb on + strandat 902.172 kb on - strandat 902.172 kb on - strandat 902.172 kb on - strandat 902.196 kb on - strandat 902.198 kb on - strandat 902.198 kb on - strandat 902.199 kb on + strandat 902.199 kb on + strandat 902.199 kb on + strandat 902.199 kb on + strandat 902.247 kb on + strandat 902.281 kb on - strandat 902.282 kb on + strandat 902.287 kb on + strandat 902.332 kb on - strandat 902.413 kb on + strand, within MMJJ_RS04815at 902.471 kb on + strand, within MMJJ_RS04815at 902.479 kb on - strand, within MMJJ_RS04815at 902.494 kb on + strand, within MMJJ_RS04815at 902.498 kb on + strand, within MMJJ_RS04815at 902.567 kb on - strand, within MMJJ_RS04815at 902.641 kb on + strand, within MMJJ_RS04815at 902.686 kb on + strand, within MMJJ_RS04815at 902.759 kb on - strand, within MMJJ_RS04815at 902.759 kb on - strand, within MMJJ_RS04815at 902.839 kb on + strand, within MMJJ_RS04815at 902.872 kb on - strand, within MMJJ_RS04815at 902.914 kb on - strandat 902.915 kb on + strandat 902.915 kb on + strandat 902.918 kb on - strandat 902.936 kb on - strandat 902.937 kb on + strandat 902.946 kb on - strandat 902.946 kb on - strandat 902.947 kb on + strandat 902.947 kb on + strandat 902.947 kb on + strandat 902.953 kb on + strandat 902.957 kb on - strandat 902.957 kb on - strandat 902.961 kb on - strandat 902.961 kb on - strandat 902.961 kb on - strandat 902.962 kb on + strandat 902.962 kb on + strandat 902.962 kb on + strandat 903.006 kb on - strandat 903.109 kb on - strand, within MMJJ_RS04820at 903.213 kb on - strand, within MMJJ_RS04820at 903.214 kb on + strand, within MMJJ_RS04820at 903.221 kb on + strand, within MMJJ_RS04820at 903.248 kb on + strand, within MMJJ_RS04820at 903.313 kb on - strand, within MMJJ_RS04820at 903.344 kb on - strand, within MMJJ_RS04820at 903.371 kb on - strand, within MMJJ_RS04820at 903.371 kb on - strand, within MMJJ_RS04820at 903.385 kb on + strand, within MMJJ_RS04820at 903.421 kb on + strand, within MMJJ_RS04820at 903.439 kb on + strand, within MMJJ_RS04820at 903.491 kb on - strand, within MMJJ_RS04820at 903.491 kb on - strand, within MMJJ_RS04820at 903.491 kb on - strand, within MMJJ_RS04820at 903.492 kb on + strand, within MMJJ_RS04820at 903.492 kb on + strand, within MMJJ_RS04820at 903.494 kb on - strand, within MMJJ_RS04820at 903.496 kb on - strand, within MMJJ_RS04820at 903.496 kb on - strand, within MMJJ_RS04820at 903.496 kb on - strand, within MMJJ_RS04820at 903.519 kb on - strand, within MMJJ_RS04820at 903.520 kb on + strand, within MMJJ_RS04820at 903.687 kb on - strand, within MMJJ_RS04820at 903.697 kb on - strand, within MMJJ_RS04820at 903.697 kb on - strand, within MMJJ_RS04820at 903.699 kb on - strand, within MMJJ_RS04820at 903.699 kb on - strand, within MMJJ_RS04820at 903.700 kb on + strand, within MMJJ_RS04820at 903.700 kb on + strand, within MMJJ_RS04820at 903.702 kb on + strandat 903.734 kb on - strandat 903.736 kb on - strandat 903.736 kb on - strandat 903.737 kb on + strandat 903.737 kb on + strandat 903.740 kb on + strandat 903.748 kb on - strandat 903.748 kb on - strandat 903.749 kb on + strandat 903.752 kb on - strandat 903.752 kb on - strandat 903.806 kb on - strandat 903.810 kb on + strandat 903.813 kb on + strandat 903.852 kb on - strandat 903.887 kb on - strandat 903.933 kb on - strand, within MMJJ_RS04825at 903.934 kb on + strand, within MMJJ_RS04825

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Per-strain Table

Position Strand Gene LocusTag Fraction formate McAA folic dropout
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901,293 - MMJJ_RS04805 0.84 -0.3
901,499 + -1.5
901,526 - -0.3
901,527 + +0.6
901,533 - -2.5
901,563 + -0.6
901,599 - +0.3
901,620 - +0.4
901,625 - -0.4
901,686 + MMJJ_RS04810 0.18 -0.2
901,754 - MMJJ_RS04810 0.31 -0.2
901,755 + MMJJ_RS04810 0.31 -1.2
901,761 - MMJJ_RS04810 0.32 -1.2
901,762 + MMJJ_RS04810 0.33 -0.4
901,776 + MMJJ_RS04810 0.35 -1.9
901,805 - MMJJ_RS04810 0.41 +0.5
901,805 - +0.5
901,805 - MMJJ_RS04810 0.41 +0.1
901,805 - MMJJ_RS04810 0.41 +0.4
901,808 - MMJJ_RS04810 0.41 -0.5
901,809 + MMJJ_RS04810 0.42 -0.1
901,811 - MMJJ_RS04810 0.42 +0.2
901,811 - MMJJ_RS04810 0.42 -0.7
901,889 - MMJJ_RS04810 0.57 -1.1
901,889 - MMJJ_RS04810 0.57 -1.8
901,889 - MMJJ_RS04810 0.57 +0.3
901,895 - MMJJ_RS04810 0.58 -0.0
901,896 + MMJJ_RS04810 0.58 -0.9
901,896 + MMJJ_RS04810 0.58 +1.0
901,977 + MMJJ_RS04810 0.74 +0.8
902,048 - MMJJ_RS04810 0.87 -0.8
902,076 - +0.4
902,076 - +0.0
902,077 + +1.3
902,102 - -0.8
902,113 - -1.2
902,113 - -0.0
902,114 + +0.9
902,114 + +0.6
902,115 - +0.8
902,115 - +0.9
902,143 + +1.5
902,147 + +0.4
902,170 - +0.0
902,171 + -0.5
902,171 + +0.3
902,172 - -0.4
902,172 - +0.5
902,172 - -0.5
902,196 - +0.4
902,198 - +1.1
902,198 - -0.3
902,199 + +0.4
902,199 + -1.0
902,199 + -0.3
902,199 + +0.4
902,247 + +0.2
902,281 - -0.6
902,282 + -0.8
902,287 + +0.3
902,332 - -0.5
902,413 + MMJJ_RS04815 0.20 +0.3
902,471 + MMJJ_RS04815 0.29 +0.5
902,479 - MMJJ_RS04815 0.30 -1.1
902,494 + MMJJ_RS04815 0.32 +0.5
902,498 + MMJJ_RS04815 0.33 +0.1
902,567 - MMJJ_RS04815 0.43 +0.4
902,641 + MMJJ_RS04815 0.55 +0.6
902,686 + MMJJ_RS04815 0.61 +1.2
902,759 - MMJJ_RS04815 0.73 +0.1
902,759 - MMJJ_RS04815 0.73 -0.4
902,839 + MMJJ_RS04815 0.85 +0.1
902,872 - MMJJ_RS04815 0.90 +1.5
902,914 - +1.5
902,915 + -0.0
902,915 + +0.4
902,918 - +0.7
902,936 - +0.5
902,937 + +0.8
902,946 - +0.0
902,946 - -0.5
902,947 + +0.4
902,947 + +0.0
902,947 + -0.1
902,953 + +0.3
902,957 - +1.1
902,957 - +0.2
902,961 - +0.2
902,961 - -0.1
902,961 - -0.2
902,962 + +0.3
902,962 + -0.5
902,962 + +0.1
903,006 - +2.4
903,109 - MMJJ_RS04820 0.17 +0.8
903,213 - MMJJ_RS04820 0.30 -0.9
903,214 + MMJJ_RS04820 0.30 +0.3
903,221 + MMJJ_RS04820 0.31 -0.8
903,248 + MMJJ_RS04820 0.34 +0.6
903,313 - MMJJ_RS04820 0.42 -0.3
903,344 - MMJJ_RS04820 0.46 +0.6
903,371 - MMJJ_RS04820 0.49 -0.1
903,371 - MMJJ_RS04820 0.49 -1.1
903,385 + MMJJ_RS04820 0.51 -0.3
903,421 + MMJJ_RS04820 0.55 -0.7
903,439 + MMJJ_RS04820 0.58 +0.7
903,491 - MMJJ_RS04820 0.64 +0.4
903,491 - MMJJ_RS04820 0.64 +0.4
903,491 - MMJJ_RS04820 0.64 +0.6
903,492 + MMJJ_RS04820 0.64 +0.1
903,492 + MMJJ_RS04820 0.64 -1.1
903,494 - MMJJ_RS04820 0.64 +0.1
903,496 - MMJJ_RS04820 0.65 -0.9
903,496 - MMJJ_RS04820 0.65 +0.3
903,496 - MMJJ_RS04820 0.65 +0.2
903,519 - MMJJ_RS04820 0.68 -0.4
903,520 + MMJJ_RS04820 0.68 -0.6
903,687 - MMJJ_RS04820 0.88 +0.7
903,697 - MMJJ_RS04820 0.90 +1.1
903,697 - MMJJ_RS04820 0.90 +0.8
903,699 - MMJJ_RS04820 0.90 +0.1
903,699 - MMJJ_RS04820 0.90 -0.9
903,700 + MMJJ_RS04820 0.90 +0.3
903,700 + MMJJ_RS04820 0.90 -0.3
903,702 + -1.5
903,734 - +0.7
903,736 - +0.5
903,736 - -1.0
903,737 + -0.4
903,737 + -0.6
903,740 + +1.0
903,748 - +0.1
903,748 - +0.2
903,749 + -1.1
903,752 - +0.0
903,752 - -0.1
903,806 - +0.3
903,810 + -0.1
903,813 + +0.7
903,852 - -0.3
903,887 - -0.5
903,933 - MMJJ_RS04825 0.11 +1.2
903,934 + MMJJ_RS04825 0.11 +0.2

Or see this region's nucleotide sequence