Experiment: formate McAA folic dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00260 and MMJJ_RS00265 are separated by 22 nucleotides MMJJ_RS00265 and MMJJ_RS00270 are separated by 15 nucleotides MMJJ_RS00270 and MMJJ_RS00275 are separated by 53 nucleotides MMJJ_RS00275 and MMJJ_RS00280 are separated by 16 nucleotides
MMJJ_RS00260: MMJJ_RS00260 - energy-converting hydrogenase B subunit EhbP, at 51,501 to 51,788
_RS00260
MMJJ_RS00265: MMJJ_RS00265 - TIGR02253 family HAD-type hydrolase, at 51,811 to 52,488
_RS00265
MMJJ_RS00270: MMJJ_RS00270 - adenosylcobinamide-GDP ribazoletransferase, at 52,504 to 53,277
_RS00270
MMJJ_RS00275: MMJJ_RS00275 - coenzyme F420-0:L-glutamate ligase, at 53,331 to 54,077
_RS00275
MMJJ_RS00280: MMJJ_RS00280 - shikimate dehydrogenase, at 54,094 to 54,930
_RS00280
Position (kb) 52
53
54 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 51.576 kb on + strand, within MMJJ_RS00260 at 51.589 kb on - strand, within MMJJ_RS00260 at 51.791 kb on - strand at 51.791 kb on - strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.794 kb on + strand at 51.817 kb on + strand at 51.894 kb on + strand, within MMJJ_RS00265 at 51.925 kb on + strand, within MMJJ_RS00265 at 51.947 kb on - strand, within MMJJ_RS00265 at 52.027 kb on - strand, within MMJJ_RS00265 at 52.059 kb on - strand, within MMJJ_RS00265 at 52.105 kb on + strand, within MMJJ_RS00265 at 52.113 kb on - strand, within MMJJ_RS00265 at 52.118 kb on - strand, within MMJJ_RS00265 at 52.171 kb on + strand, within MMJJ_RS00265 at 52.230 kb on - strand, within MMJJ_RS00265 at 52.231 kb on + strand, within MMJJ_RS00265 at 52.231 kb on + strand, within MMJJ_RS00265 at 52.233 kb on + strand, within MMJJ_RS00265 at 52.283 kb on + strand, within MMJJ_RS00265 at 52.305 kb on + strand, within MMJJ_RS00265 at 52.427 kb on - strand at 52.428 kb on + strand at 52.452 kb on + strand at 52.482 kb on - strand at 52.654 kb on + strand, within MMJJ_RS00270 at 52.655 kb on - strand, within MMJJ_RS00270 at 52.719 kb on + strand, within MMJJ_RS00270 at 52.723 kb on + strand, within MMJJ_RS00270 at 52.810 kb on + strand, within MMJJ_RS00270 at 52.810 kb on + strand, within MMJJ_RS00270 at 52.928 kb on + strand, within MMJJ_RS00270 at 52.934 kb on - strand, within MMJJ_RS00270 at 53.082 kb on + strand at 53.257 kb on - strand at 53.269 kb on + strand at 53.279 kb on - strand at 53.300 kb on + strand at 53.305 kb on - strand at 53.309 kb on - strand at 53.561 kb on - strand, within MMJJ_RS00275 at 53.667 kb on + strand, within MMJJ_RS00275 at 53.838 kb on - strand, within MMJJ_RS00275 at 53.839 kb on + strand, within MMJJ_RS00275 at 53.981 kb on + strand, within MMJJ_RS00275 at 54.006 kb on + strand at 54.108 kb on - strand at 54.130 kb on - strand at 54.131 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA folic dropout remove 51,576 + MMJJ_RS00260 0.26 -3.1 51,589 - MMJJ_RS00260 0.31 -2.7 51,791 - -1.8 51,791 - -0.5 51,794 + -2.4 51,794 + -1.1 51,794 + -0.5 51,794 + -2.3 51,817 + -2.8 51,894 + MMJJ_RS00265 0.12 -1.9 51,925 + MMJJ_RS00265 0.17 -2.7 51,947 - MMJJ_RS00265 0.20 -2.1 52,027 - MMJJ_RS00265 0.32 -4.3 52,059 - MMJJ_RS00265 0.37 -2.7 52,105 + MMJJ_RS00265 0.43 -1.0 52,113 - MMJJ_RS00265 0.45 -2.5 52,118 - MMJJ_RS00265 0.45 -0.4 52,171 + MMJJ_RS00265 0.53 -1.7 52,230 - MMJJ_RS00265 0.62 -1.3 52,231 + MMJJ_RS00265 0.62 -1.4 52,231 + MMJJ_RS00265 0.62 -2.7 52,233 + MMJJ_RS00265 0.62 -1.7 52,283 + MMJJ_RS00265 0.70 -3.2 52,305 + MMJJ_RS00265 0.73 +0.0 52,427 - +0.7 52,428 + -3.0 52,452 + -2.2 52,482 - -1.9 52,654 + MMJJ_RS00270 0.19 -2.7 52,655 - MMJJ_RS00270 0.20 -3.3 52,719 + MMJJ_RS00270 0.28 -3.1 52,723 + MMJJ_RS00270 0.28 -2.3 52,810 + MMJJ_RS00270 0.40 -1.4 52,810 + MMJJ_RS00270 0.40 -3.6 52,928 + MMJJ_RS00270 0.55 -3.8 52,934 - MMJJ_RS00270 0.56 -2.2 53,082 + -1.0 53,257 - -1.0 53,269 + -1.1 53,279 - +0.1 53,300 + +0.6 53,305 - -1.7 53,309 - +0.6 53,561 - MMJJ_RS00275 0.31 -3.1 53,667 + MMJJ_RS00275 0.45 -4.0 53,838 - MMJJ_RS00275 0.68 -3.2 53,839 + MMJJ_RS00275 0.68 -2.0 53,981 + MMJJ_RS00275 0.87 -3.7 54,006 + -1.7 54,108 - -0.3 54,130 - -2.3 54,131 + -1.2
Or see this region's nucleotide sequence