Experiment: R2A 23C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23515 and RR42_RS23520 are separated by 164 nucleotides RR42_RS23520 and RR42_RS23525 are separated by 20 nucleotides
RR42_RS23515: RR42_RS23515 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 601,469 to 604,054
_RS23515
RR42_RS23520: RR42_RS23520 - thioesterase, at 604,219 to 604,692
_RS23520
RR42_RS23525: RR42_RS23525 - glycosyl transferase, at 604,713 to 605,834
_RS23525
Position (kb) 604
605 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 603.327 kb on - strand, within RR42_RS23515 at 603.841 kb on + strand at 603.879 kb on - strand at 603.944 kb on + strand at 603.944 kb on + strand at 603.944 kb on + strand at 603.944 kb on + strand at 603.944 kb on + strand at 603.944 kb on + strand at 603.945 kb on - strand at 603.989 kb on + strand at 603.989 kb on + strand at 603.989 kb on + strand at 603.995 kb on + strand at 603.996 kb on - strand at 603.996 kb on - strand at 603.996 kb on - strand at 603.996 kb on - strand at 603.996 kb on - strand at 603.996 kb on - strand at 604.053 kb on - strand at 604.053 kb on - strand at 604.053 kb on - strand at 604.076 kb on + strand at 604.077 kb on - strand at 604.077 kb on - strand at 604.183 kb on - strand at 604.183 kb on - strand at 604.183 kb on - strand at 604.199 kb on + strand at 604.199 kb on + strand at 604.199 kb on + strand at 604.200 kb on - strand at 604.200 kb on - strand at 604.200 kb on - strand at 604.200 kb on - strand at 604.200 kb on - strand at 604.200 kb on - strand at 604.261 kb on + strand at 604.261 kb on + strand at 604.294 kb on - strand, within RR42_RS23520 at 604.445 kb on - strand, within RR42_RS23520 at 604.511 kb on - strand, within RR42_RS23520 at 604.598 kb on - strand, within RR42_RS23520 at 604.598 kb on - strand, within RR42_RS23520 at 604.690 kb on + strand at 604.691 kb on - strand at 604.714 kb on + strand at 604.714 kb on + strand at 604.714 kb on + strand at 604.714 kb on + strand at 604.715 kb on - strand at 604.715 kb on - strand at 604.724 kb on - strand at 604.727 kb on - strand at 604.727 kb on - strand at 604.727 kb on - strand at 604.829 kb on - strand, within RR42_RS23525 at 604.829 kb on - strand, within RR42_RS23525 at 604.829 kb on - strand, within RR42_RS23525 at 604.829 kb on - strand, within RR42_RS23525 at 604.829 kb on - strand, within RR42_RS23525 at 604.829 kb on - strand, within RR42_RS23525 at 604.831 kb on + strand, within RR42_RS23525 at 604.832 kb on - strand, within RR42_RS23525 at 604.870 kb on + strand, within RR42_RS23525 at 604.871 kb on - strand, within RR42_RS23525 at 604.871 kb on - strand, within RR42_RS23525 at 604.933 kb on + strand, within RR42_RS23525 at 604.934 kb on - strand, within RR42_RS23525 at 604.934 kb on - strand, within RR42_RS23525 at 604.934 kb on - strand, within RR42_RS23525 at 605.032 kb on + strand, within RR42_RS23525 at 605.059 kb on + strand, within RR42_RS23525 at 605.060 kb on - strand, within RR42_RS23525 at 605.060 kb on - strand, within RR42_RS23525 at 605.176 kb on + strand, within RR42_RS23525 at 605.177 kb on - strand, within RR42_RS23525 at 605.177 kb on - strand, within RR42_RS23525 at 605.230 kb on + strand, within RR42_RS23525 at 605.239 kb on + strand, within RR42_RS23525 at 605.240 kb on - strand, within RR42_RS23525 at 605.300 kb on - strand, within RR42_RS23525 at 605.300 kb on - strand, within RR42_RS23525 at 605.395 kb on + strand, within RR42_RS23525 at 605.396 kb on - strand, within RR42_RS23525 at 605.458 kb on + strand, within RR42_RS23525 at 605.459 kb on - strand, within RR42_RS23525 at 605.459 kb on - strand, within RR42_RS23525 at 605.565 kb on + strand, within RR42_RS23525 at 605.565 kb on + strand, within RR42_RS23525 at 605.565 kb on + strand, within RR42_RS23525 at 605.566 kb on - strand, within RR42_RS23525 at 605.566 kb on - strand, within RR42_RS23525 at 605.566 kb on - strand, within RR42_RS23525 at 605.569 kb on + strand, within RR42_RS23525 at 605.569 kb on + strand, within RR42_RS23525
Per-strain Table
Position Strand Gene LocusTag Fraction R2A 23C remove 603,327 - RR42_RS23515 0.72 +0.9 603,841 + +3.1 603,879 - +1.5 603,944 + -1.9 603,944 + -1.8 603,944 + +1.4 603,944 + -0.9 603,944 + -1.7 603,944 + -0.6 603,945 - -0.7 603,989 + -0.8 603,989 + -1.3 603,989 + +0.1 603,995 + +0.0 603,996 - -0.5 603,996 - -0.6 603,996 - -0.1 603,996 - +0.1 603,996 - -0.6 603,996 - -0.3 604,053 - +0.4 604,053 - -1.0 604,053 - +0.1 604,076 + -0.8 604,077 - -1.1 604,077 - +1.1 604,183 - -0.1 604,183 - +0.2 604,183 - +0.6 604,199 + -0.3 604,199 + -1.5 604,199 + -0.1 604,200 - +0.9 604,200 - +1.2 604,200 - -0.4 604,200 - -0.1 604,200 - +0.2 604,200 - -0.9 604,261 + +0.1 604,261 + -0.7 604,294 - RR42_RS23520 0.16 +1.5 604,445 - RR42_RS23520 0.48 +0.6 604,511 - RR42_RS23520 0.62 +0.1 604,598 - RR42_RS23520 0.80 -0.4 604,598 - RR42_RS23520 0.80 +1.1 604,690 + -0.5 604,691 - +0.2 604,714 + -1.6 604,714 + -1.5 604,714 + -0.4 604,714 + -1.1 604,715 - +0.7 604,715 - +0.7 604,724 - +0.4 604,727 - +0.2 604,727 - +0.3 604,727 - +0.0 604,829 - RR42_RS23525 0.10 -0.2 604,829 - RR42_RS23525 0.10 +0.2 604,829 - RR42_RS23525 0.10 -0.2 604,829 - RR42_RS23525 0.10 +0.4 604,829 - RR42_RS23525 0.10 -0.8 604,829 - RR42_RS23525 0.10 +0.0 604,831 + RR42_RS23525 0.11 +0.0 604,832 - RR42_RS23525 0.11 -1.3 604,870 + RR42_RS23525 0.14 -0.0 604,871 - RR42_RS23525 0.14 +0.7 604,871 - RR42_RS23525 0.14 +0.1 604,933 + RR42_RS23525 0.20 +0.1 604,934 - RR42_RS23525 0.20 +0.0 604,934 - RR42_RS23525 0.20 -0.6 604,934 - RR42_RS23525 0.20 -0.3 605,032 + RR42_RS23525 0.28 +0.4 605,059 + RR42_RS23525 0.31 +0.1 605,060 - RR42_RS23525 0.31 -0.0 605,060 - RR42_RS23525 0.31 +1.0 605,176 + RR42_RS23525 0.41 -0.4 605,177 - RR42_RS23525 0.41 -1.5 605,177 - RR42_RS23525 0.41 +0.9 605,230 + RR42_RS23525 0.46 +0.4 605,239 + RR42_RS23525 0.47 +3.7 605,240 - RR42_RS23525 0.47 +0.2 605,300 - RR42_RS23525 0.52 -0.0 605,300 - RR42_RS23525 0.52 +0.1 605,395 + RR42_RS23525 0.61 -0.8 605,396 - RR42_RS23525 0.61 +0.8 605,458 + RR42_RS23525 0.66 -1.1 605,459 - RR42_RS23525 0.66 +0.1 605,459 - RR42_RS23525 0.66 +2.5 605,565 + RR42_RS23525 0.76 +0.3 605,565 + RR42_RS23525 0.76 -0.7 605,565 + RR42_RS23525 0.76 -1.7 605,566 - RR42_RS23525 0.76 +0.7 605,566 - RR42_RS23525 0.76 +0.2 605,566 - RR42_RS23525 0.76 +1.3 605,569 + RR42_RS23525 0.76 -0.2 605,569 + RR42_RS23525 0.76 +0.5
Or see this region's nucleotide sequence