Experiment: R2A 23C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15805 and RR42_RS15810 overlap by 4 nucleotides RR42_RS15810 and RR42_RS15815 are separated by 33 nucleotides RR42_RS15815 and RR42_RS15820 are separated by 10 nucleotides
RR42_RS15805: RR42_RS15805 - spore coat protein, at 3,415,647 to 3,416,795
_RS15805
RR42_RS15810: RR42_RS15810 - UDP-N-acetylglucosamine 4,6-dehydratase, at 3,416,792 to 3,417,793
_RS15810
RR42_RS15815: RR42_RS15815 - NAD-dependent dehydratase, at 3,417,827 to 3,418,777
_RS15815
RR42_RS15820: RR42_RS15820 - UDP-glucose 6-dehydrogenase, at 3,418,788 to 3,420,149
_RS15820
Position (kb)
3416
3417
3418 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3416.348 kb on - strand, within RR42_RS15805 at 3416.866 kb on - strand at 3417.074 kb on + strand, within RR42_RS15810 at 3417.335 kb on + strand, within RR42_RS15810 at 3417.519 kb on + strand, within RR42_RS15810 at 3417.564 kb on + strand, within RR42_RS15810 at 3417.565 kb on - strand, within RR42_RS15810 at 3417.828 kb on + strand at 3417.829 kb on - strand at 3417.830 kb on + strand at 3417.830 kb on + strand at 3417.831 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A 23C remove 3,416,348 - RR42_RS15805 0.61 -1.0 3,416,866 - -1.8 3,417,074 + RR42_RS15810 0.28 -0.9 3,417,335 + RR42_RS15810 0.54 -2.8 3,417,519 + RR42_RS15810 0.73 -0.2 3,417,564 + RR42_RS15810 0.77 -1.6 3,417,565 - RR42_RS15810 0.77 -0.6 3,417,828 + +0.3 3,417,829 - -0.1 3,417,830 + -1.1 3,417,830 + -0.3 3,417,831 - +0.5
Or see this region's nucleotide sequence