Experiment: R2A 23C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06240 and RR42_RS06245 are separated by 14 nucleotides RR42_RS06245 and RR42_RS06250 are separated by 150 nucleotides RR42_RS06250 and RR42_RS06255 are separated by 45 nucleotides
RR42_RS06240: RR42_RS06240 - tryptophan--tRNA ligase, at 1,369,147 to 1,370,349
_RS06240
RR42_RS06245: RR42_RS06245 - SAM-dependent methyltransferase, at 1,370,364 to 1,370,942
_RS06245
RR42_RS06250: RR42_RS06250 - dihydrodipicolinate synthase, at 1,371,093 to 1,371,977
_RS06250
RR42_RS06255: RR42_RS06255 - lipoprotein, at 1,372,023 to 1,373,234
_RS06255
Position (kb)
1371
1372 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1370.646 kb on + strand, within RR42_RS06245 at 1370.647 kb on - strand, within RR42_RS06245 at 1370.824 kb on - strand, within RR42_RS06245 at 1370.870 kb on + strand, within RR42_RS06245 at 1370.891 kb on + strand at 1370.891 kb on + strand at 1370.892 kb on - strand at 1370.892 kb on - strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.894 kb on - strand at 1370.894 kb on - strand at 1370.894 kb on - strand at 1371.417 kb on + strand, within RR42_RS06250 at 1371.417 kb on + strand, within RR42_RS06250 at 1371.495 kb on + strand, within RR42_RS06250 at 1371.648 kb on + strand, within RR42_RS06250 at 1371.648 kb on + strand, within RR42_RS06250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A 23C remove 1,370,646 + RR42_RS06245 0.49 -1.1 1,370,647 - RR42_RS06245 0.49 -0.8 1,370,824 - RR42_RS06245 0.79 +0.5 1,370,870 + RR42_RS06245 0.87 +0.3 1,370,891 + -0.1 1,370,891 + +0.1 1,370,892 - +0.2 1,370,892 - -1.4 1,370,893 + +0.0 1,370,893 + -0.8 1,370,893 + +0.0 1,370,893 + -0.4 1,370,893 + +0.5 1,370,894 - -0.3 1,370,894 - -0.1 1,370,894 - +0.5 1,371,417 + RR42_RS06250 0.37 +1.9 1,371,417 + RR42_RS06250 0.37 +1.3 1,371,495 + RR42_RS06250 0.45 -0.1 1,371,648 + RR42_RS06250 0.63 +1.3 1,371,648 + RR42_RS06250 0.63 -3.2
Or see this region's nucleotide sequence