Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_81

Experiment: Nalidixic 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_80 and Pf1N1B4_81 are separated by 168 nucleotidesPf1N1B4_81 and Pf1N1B4_82 are separated by 441 nucleotides Pf1N1B4_80 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 82,561 to 83,844 _80 Pf1N1B4_81 - Putative sulfate permease, at 84,013 to 84,798 _81 Pf1N1B4_82 - Mobile element protein, at 85,240 to 86,181 _82 Position (kb) 84 85Strain fitness (log2 ratio) -2 -1 0 1 2at 83.040 kb on + strand, within Pf1N1B4_80at 83.041 kb on - strand, within Pf1N1B4_80at 83.165 kb on + strand, within Pf1N1B4_80at 83.166 kb on - strand, within Pf1N1B4_80at 83.364 kb on + strand, within Pf1N1B4_80at 83.364 kb on + strandat 83.365 kb on - strand, within Pf1N1B4_80at 83.365 kb on - strand, within Pf1N1B4_80at 83.366 kb on + strand, within Pf1N1B4_80at 83.423 kb on - strand, within Pf1N1B4_80at 83.423 kb on - strand, within Pf1N1B4_80at 83.450 kb on + strand, within Pf1N1B4_80at 83.544 kb on + strand, within Pf1N1B4_80at 83.545 kb on - strand, within Pf1N1B4_80at 83.717 kb on + strandat 83.717 kb on + strandat 83.718 kb on - strandat 83.840 kb on - strandat 83.894 kb on - strandat 83.905 kb on + strandat 83.950 kb on + strandat 83.999 kb on + strandat 83.999 kb on + strandat 83.999 kb on + strandat 83.999 kb on + strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.000 kb on - strandat 84.001 kb on + strandat 84.001 kb on + strandat 84.030 kb on - strandat 84.110 kb on + strand, within Pf1N1B4_81at 84.140 kb on + strand, within Pf1N1B4_81at 84.163 kb on - strand, within Pf1N1B4_81at 84.383 kb on + strand, within Pf1N1B4_81at 84.460 kb on + strand, within Pf1N1B4_81at 84.647 kb on + strand, within Pf1N1B4_81at 84.855 kb on + strandat 84.858 kb on - strandat 84.866 kb on - strandat 84.881 kb on - strandat 84.883 kb on - strandat 84.981 kb on + strandat 84.981 kb on + strandat 85.035 kb on - strandat 85.035 kb on - strandat 85.035 kb on - strandat 85.035 kb on - strandat 85.035 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml
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83,040 + Pf1N1B4_80 0.37 -1.2
83,041 - Pf1N1B4_80 0.37 -0.8
83,165 + Pf1N1B4_80 0.47 +0.5
83,166 - Pf1N1B4_80 0.47 +1.9
83,364 + Pf1N1B4_80 0.63 +0.8
83,364 + +0.2
83,365 - Pf1N1B4_80 0.63 -0.4
83,365 - Pf1N1B4_80 0.63 -0.6
83,366 + Pf1N1B4_80 0.63 +0.8
83,423 - Pf1N1B4_80 0.67 -0.2
83,423 - Pf1N1B4_80 0.67 +0.8
83,450 + Pf1N1B4_80 0.69 +0.5
83,544 + Pf1N1B4_80 0.77 +0.6
83,545 - Pf1N1B4_80 0.77 -0.6
83,717 + +0.5
83,717 + +0.1
83,718 - -0.3
83,840 - -0.0
83,894 - +0.2
83,905 + +0.8
83,950 + +0.9
83,999 + -0.5
83,999 + -0.6
83,999 + -0.6
83,999 + -2.2
84,000 - -1.2
84,000 - -0.2
84,000 - -0.2
84,000 - +0.8
84,000 - +0.3
84,000 - -0.8
84,000 - -0.2
84,000 - -0.2
84,000 - -0.5
84,000 - +2.2
84,000 - +0.8
84,000 - +0.6
84,000 - +0.8
84,000 - +0.3
84,000 - +0.0
84,000 - -0.2
84,001 + -0.8
84,001 + +0.2
84,030 - +2.1
84,110 + Pf1N1B4_81 0.12 +1.9
84,140 + Pf1N1B4_81 0.16 +0.8
84,163 - Pf1N1B4_81 0.19 +0.5
84,383 + Pf1N1B4_81 0.47 -0.0
84,460 + Pf1N1B4_81 0.57 +1.3
84,647 + Pf1N1B4_81 0.81 +0.8
84,855 + -0.2
84,858 - -0.8
84,866 - +1.8
84,881 - +0.8
84,883 - +1.2
84,981 + -2.1
84,981 + +0.9
85,035 - +1.2
85,035 - -0.2
85,035 - -0.5
85,035 - -1.7
85,035 - -0.6

Or see this region's nucleotide sequence