Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_80 and Pf1N1B4_81 are separated by 168 nucleotides Pf1N1B4_81 and Pf1N1B4_82 are separated by 441 nucleotides
Pf1N1B4_80 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 82,561 to 83,844
_80
Pf1N1B4_81 - Putative sulfate permease, at 84,013 to 84,798
_81
Pf1N1B4_82 - Mobile element protein, at 85,240 to 86,181
_82
Position (kb)
84
85 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 83.040 kb on + strand, within Pf1N1B4_80 at 83.041 kb on - strand, within Pf1N1B4_80 at 83.165 kb on + strand, within Pf1N1B4_80 at 83.166 kb on - strand, within Pf1N1B4_80 at 83.364 kb on + strand, within Pf1N1B4_80 at 83.364 kb on + strand at 83.365 kb on - strand, within Pf1N1B4_80 at 83.365 kb on - strand, within Pf1N1B4_80 at 83.366 kb on + strand, within Pf1N1B4_80 at 83.423 kb on - strand, within Pf1N1B4_80 at 83.423 kb on - strand, within Pf1N1B4_80 at 83.450 kb on + strand, within Pf1N1B4_80 at 83.544 kb on + strand, within Pf1N1B4_80 at 83.545 kb on - strand, within Pf1N1B4_80 at 83.717 kb on + strand at 83.717 kb on + strand at 83.718 kb on - strand at 83.840 kb on - strand at 83.894 kb on - strand at 83.905 kb on + strand at 83.950 kb on + strand at 83.999 kb on + strand at 83.999 kb on + strand at 83.999 kb on + strand at 83.999 kb on + strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.000 kb on - strand at 84.001 kb on + strand at 84.001 kb on + strand at 84.030 kb on - strand at 84.110 kb on + strand, within Pf1N1B4_81 at 84.140 kb on + strand, within Pf1N1B4_81 at 84.163 kb on - strand, within Pf1N1B4_81 at 84.383 kb on + strand, within Pf1N1B4_81 at 84.460 kb on + strand, within Pf1N1B4_81 at 84.647 kb on + strand, within Pf1N1B4_81 at 84.855 kb on + strand at 84.858 kb on - strand at 84.866 kb on - strand at 84.881 kb on - strand at 84.883 kb on - strand at 84.981 kb on + strand at 84.981 kb on + strand at 85.035 kb on - strand at 85.035 kb on - strand at 85.035 kb on - strand at 85.035 kb on - strand at 85.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 83,040 + Pf1N1B4_80 0.37 -1.2 83,041 - Pf1N1B4_80 0.37 -0.8 83,165 + Pf1N1B4_80 0.47 +0.5 83,166 - Pf1N1B4_80 0.47 +1.9 83,364 + Pf1N1B4_80 0.63 +0.8 83,364 + +0.2 83,365 - Pf1N1B4_80 0.63 -0.4 83,365 - Pf1N1B4_80 0.63 -0.6 83,366 + Pf1N1B4_80 0.63 +0.8 83,423 - Pf1N1B4_80 0.67 -0.2 83,423 - Pf1N1B4_80 0.67 +0.8 83,450 + Pf1N1B4_80 0.69 +0.5 83,544 + Pf1N1B4_80 0.77 +0.6 83,545 - Pf1N1B4_80 0.77 -0.6 83,717 + +0.5 83,717 + +0.1 83,718 - -0.3 83,840 - -0.0 83,894 - +0.2 83,905 + +0.8 83,950 + +0.9 83,999 + -0.5 83,999 + -0.6 83,999 + -0.6 83,999 + -2.2 84,000 - -1.2 84,000 - -0.2 84,000 - -0.2 84,000 - +0.8 84,000 - +0.3 84,000 - -0.8 84,000 - -0.2 84,000 - -0.2 84,000 - -0.5 84,000 - +2.2 84,000 - +0.8 84,000 - +0.6 84,000 - +0.8 84,000 - +0.3 84,000 - +0.0 84,000 - -0.2 84,001 + -0.8 84,001 + +0.2 84,030 - +2.1 84,110 + Pf1N1B4_81 0.12 +1.9 84,140 + Pf1N1B4_81 0.16 +0.8 84,163 - Pf1N1B4_81 0.19 +0.5 84,383 + Pf1N1B4_81 0.47 -0.0 84,460 + Pf1N1B4_81 0.57 +1.3 84,647 + Pf1N1B4_81 0.81 +0.8 84,855 + -0.2 84,858 - -0.8 84,866 - +1.8 84,881 - +0.8 84,883 - +1.2 84,981 + -2.1 84,981 + +0.9 85,035 - +1.2 85,035 - -0.2 85,035 - -0.5 85,035 - -1.7 85,035 - -0.6
Or see this region's nucleotide sequence