Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_694 and Pf1N1B4_695 are separated by 218 nucleotides Pf1N1B4_695 and Pf1N1B4_696 overlap by 4 nucleotides
Pf1N1B4_694 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 792,211 to 793,314
_694
Pf1N1B4_695 - Aldehyde dehydrogenase (EC 1.2.1.3), at 793,533 to 794,981
_695
Pf1N1B4_696 - Acetolactate synthase, large subunit (EC 2.2.1.6), at 794,978 to 796,615
_696
Position (kb) 793
794
795 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 792.539 kb on + strand, within Pf1N1B4_694 at 792.540 kb on - strand, within Pf1N1B4_694 at 792.581 kb on + strand, within Pf1N1B4_694 at 792.581 kb on + strand, within Pf1N1B4_694 at 792.582 kb on - strand, within Pf1N1B4_694 at 792.666 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.819 kb on - strand, within Pf1N1B4_694 at 792.819 kb on - strand, within Pf1N1B4_694 at 792.855 kb on - strand, within Pf1N1B4_694 at 793.025 kb on + strand, within Pf1N1B4_694 at 793.109 kb on + strand, within Pf1N1B4_694 at 793.110 kb on - strand, within Pf1N1B4_694 at 793.110 kb on - strand, within Pf1N1B4_694 at 793.337 kb on - strand at 793.337 kb on - strand at 793.337 kb on - strand at 793.340 kb on + strand at 793.340 kb on + strand at 793.340 kb on + strand at 793.341 kb on - strand at 793.341 kb on - strand at 793.801 kb on - strand, within Pf1N1B4_695 at 793.914 kb on - strand, within Pf1N1B4_695 at 794.196 kb on + strand, within Pf1N1B4_695 at 794.450 kb on + strand, within Pf1N1B4_695 at 794.457 kb on + strand, within Pf1N1B4_695 at 794.641 kb on + strand at 794.642 kb on - strand, within Pf1N1B4_695 at 794.645 kb on - strand, within Pf1N1B4_695 at 794.645 kb on - strand, within Pf1N1B4_695 at 794.950 kb on + strand at 794.950 kb on + strand at 794.950 kb on + strand at 794.951 kb on - strand at 794.951 kb on - strand at 794.951 kb on - strand at 794.966 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.142 kb on - strand, within Pf1N1B4_696 at 795.142 kb on - strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.202 kb on - strand, within Pf1N1B4_696 at 795.453 kb on + strand, within Pf1N1B4_696 at 795.701 kb on - strand, within Pf1N1B4_696
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 792,539 + Pf1N1B4_694 0.30 -1.1 792,540 - Pf1N1B4_694 0.30 -0.1 792,581 + Pf1N1B4_694 0.34 +0.9 792,581 + Pf1N1B4_694 0.34 -0.3 792,582 - Pf1N1B4_694 0.34 +0.6 792,666 + Pf1N1B4_694 0.41 +1.7 792,818 + Pf1N1B4_694 0.55 -1.1 792,818 + Pf1N1B4_694 0.55 +1.5 792,818 + Pf1N1B4_694 0.55 +0.7 792,818 + Pf1N1B4_694 0.55 +0.3 792,819 - Pf1N1B4_694 0.55 -1.7 792,819 - Pf1N1B4_694 0.55 -2.0 792,855 - Pf1N1B4_694 0.58 -0.4 793,025 + Pf1N1B4_694 0.74 +0.9 793,109 + Pf1N1B4_694 0.81 +0.2 793,110 - Pf1N1B4_694 0.81 -1.9 793,110 - Pf1N1B4_694 0.81 -0.7 793,337 - +0.8 793,337 - +0.7 793,337 - +0.3 793,340 + +0.3 793,340 + +0.0 793,340 + +1.3 793,341 - -0.8 793,341 - +0.7 793,801 - Pf1N1B4_695 0.18 -0.5 793,914 - Pf1N1B4_695 0.26 -2.1 794,196 + Pf1N1B4_695 0.46 -1.6 794,450 + Pf1N1B4_695 0.63 -1.6 794,457 + Pf1N1B4_695 0.64 -0.6 794,641 + -0.6 794,642 - Pf1N1B4_695 0.77 -1.1 794,645 - Pf1N1B4_695 0.77 +0.9 794,645 - Pf1N1B4_695 0.77 -0.5 794,950 + -0.1 794,950 + +1.3 794,950 + -2.5 794,951 - -1.9 794,951 - +0.9 794,951 - -0.9 794,966 + -2.4 795,141 + -0.1 795,141 + +0.1 795,141 + -1.6 795,141 + +0.9 795,141 + +0.1 795,141 + +0.4 795,141 + -2.9 795,142 - Pf1N1B4_696 0.10 +0.4 795,142 - Pf1N1B4_696 0.10 -0.5 795,183 + Pf1N1B4_696 0.13 +0.9 795,183 + Pf1N1B4_696 0.13 -0.6 795,183 + Pf1N1B4_696 0.13 -0.1 795,183 + Pf1N1B4_696 0.13 -0.2 795,183 + Pf1N1B4_696 0.13 +2.3 795,183 + Pf1N1B4_696 0.13 -1.6 795,184 - Pf1N1B4_696 0.13 +0.9 795,184 - Pf1N1B4_696 0.13 -0.8 795,184 - Pf1N1B4_696 0.13 -0.2 795,184 - Pf1N1B4_696 0.13 +0.2 795,184 - Pf1N1B4_696 0.13 -0.6 795,184 - Pf1N1B4_696 0.13 -0.1 795,202 - Pf1N1B4_696 0.14 -1.4 795,453 + Pf1N1B4_696 0.29 -0.1 795,701 - Pf1N1B4_696 0.44 +0.6
Or see this region's nucleotide sequence