Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5174 and Pf1N1B4_628 are separated by 63 nucleotides Pf1N1B4_628 and Pf1N1B4_629 are separated by 13 nucleotides Pf1N1B4_629 and Pf1N1B4_630 are separated by 166 nucleotides
Pf1N1B4_5174 - tRNA-Lys-TTT, at 725,744 to 725,816
_5174
Pf1N1B4_628 - Queuosine Biosynthesis QueC ATPase, at 725,880 to 726,560
_628
Pf1N1B4_629 - Queuosine Biosynthesis QueE Radical SAM, at 726,574 to 727,221
_629
Pf1N1B4_630 - TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain, at 727,388 to 728,152
_630
Position (kb) 726
727
728 Strain fitness (log2 ratio) -2
-1
0
1
2 at 725.576 kb on - strand at 725.576 kb on - strand at 725.576 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.887 kb on + strand at 725.887 kb on + strand at 725.955 kb on - strand, within Pf1N1B4_628 at 726.096 kb on + strand, within Pf1N1B4_628 at 726.097 kb on - strand, within Pf1N1B4_628 at 726.097 kb on - strand, within Pf1N1B4_628 at 726.176 kb on - strand, within Pf1N1B4_628 at 726.176 kb on - strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.320 kb on + strand, within Pf1N1B4_628 at 726.320 kb on + strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.446 kb on - strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.464 kb on - strand, within Pf1N1B4_628 at 726.464 kb on - strand, within Pf1N1B4_628 at 726.602 kb on + strand at 726.602 kb on + strand at 726.602 kb on + strand at 726.603 kb on - strand at 726.603 kb on - strand at 726.603 kb on - strand at 726.603 kb on - strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.606 kb on + strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.607 kb on - strand at 726.761 kb on + strand, within Pf1N1B4_629 at 726.791 kb on - strand, within Pf1N1B4_629 at 727.019 kb on + strand, within Pf1N1B4_629 at 727.020 kb on - strand, within Pf1N1B4_629 at 727.221 kb on + strand at 727.221 kb on + strand at 727.243 kb on + strand at 727.243 kb on + strand at 727.243 kb on + strand at 727.244 kb on - strand at 727.244 kb on - strand at 727.318 kb on - strand at 727.389 kb on + strand at 727.389 kb on + strand at 727.389 kb on + strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.390 kb on - strand at 727.519 kb on - strand, within Pf1N1B4_630 at 727.740 kb on + strand at 727.740 kb on + strand, within Pf1N1B4_630 at 727.740 kb on + strand, within Pf1N1B4_630 at 727.740 kb on + strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.741 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.744 kb on - strand, within Pf1N1B4_630 at 727.867 kb on + strand, within Pf1N1B4_630 at 727.901 kb on + strand, within Pf1N1B4_630 at 727.902 kb on - strand, within Pf1N1B4_630 at 728.077 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.114 kb on - strand at 728.129 kb on - strand at 728.129 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand at 728.205 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 725,576 - +1.1 725,576 - +2.5 725,576 - +1.7 725,715 - +1.5 725,715 - +1.7 725,715 - -1.9 725,715 - +0.3 725,887 + -0.1 725,887 + -1.1 725,955 - Pf1N1B4_628 0.11 +2.5 726,096 + Pf1N1B4_628 0.32 -0.4 726,097 - Pf1N1B4_628 0.32 -1.6 726,097 - Pf1N1B4_628 0.32 +0.2 726,176 - Pf1N1B4_628 0.43 -0.6 726,176 - Pf1N1B4_628 0.43 -0.4 726,274 + Pf1N1B4_628 0.58 -0.9 726,274 + Pf1N1B4_628 0.58 +0.7 726,274 + Pf1N1B4_628 0.58 +1.1 726,274 + Pf1N1B4_628 0.58 +0.5 726,275 - Pf1N1B4_628 0.58 +1.2 726,275 - Pf1N1B4_628 0.58 +0.2 726,275 - Pf1N1B4_628 0.58 +0.5 726,320 + Pf1N1B4_628 0.65 +1.2 726,320 + Pf1N1B4_628 0.65 -0.3 726,321 - Pf1N1B4_628 0.65 -2.1 726,321 - Pf1N1B4_628 0.65 -0.1 726,321 - Pf1N1B4_628 0.65 +0.2 726,321 - Pf1N1B4_628 0.65 -1.9 726,321 - Pf1N1B4_628 0.65 +0.9 726,321 - Pf1N1B4_628 0.65 +1.5 726,446 - Pf1N1B4_628 0.83 +1.9 726,463 + Pf1N1B4_628 0.86 -1.2 726,463 + Pf1N1B4_628 0.86 -1.9 726,463 + Pf1N1B4_628 0.86 -0.1 726,464 - Pf1N1B4_628 0.86 -0.9 726,464 - Pf1N1B4_628 0.86 +1.1 726,602 + +0.4 726,602 + -0.6 726,602 + -2.1 726,603 - +0.4 726,603 - -1.4 726,603 - -0.1 726,603 - +0.4 726,606 + -1.6 726,606 + +0.1 726,606 + +0.8 726,606 + +0.9 726,606 + -0.5 726,606 + +0.2 726,607 - +0.4 726,607 - +1.5 726,607 - -1.4 726,607 - +1.3 726,607 - -0.1 726,607 - +0.2 726,607 - -2.2 726,607 - +0.9 726,607 - +0.9 726,607 - +0.9 726,607 - +0.9 726,761 + Pf1N1B4_629 0.29 -1.4 726,791 - Pf1N1B4_629 0.33 -1.4 727,019 + Pf1N1B4_629 0.69 -1.2 727,020 - Pf1N1B4_629 0.69 +0.9 727,221 + +0.9 727,221 + +1.8 727,243 + -0.8 727,243 + +0.4 727,243 + -0.3 727,244 - -0.4 727,244 - +0.5 727,318 - +0.9 727,389 + +0.5 727,389 + -0.3 727,389 + +0.5 727,390 - -1.9 727,390 - -0.3 727,390 - -0.2 727,390 - -0.6 727,390 - +0.0 727,390 - +1.8 727,390 - -1.6 727,390 - +0.9 727,519 - Pf1N1B4_630 0.17 -0.0 727,740 + +0.9 727,740 + Pf1N1B4_630 0.46 -0.5 727,740 + Pf1N1B4_630 0.46 -1.4 727,740 + Pf1N1B4_630 0.46 +1.9 727,741 - Pf1N1B4_630 0.46 -0.1 727,741 - Pf1N1B4_630 0.46 -1.6 727,741 - Pf1N1B4_630 0.46 +0.1 727,741 - Pf1N1B4_630 0.46 -0.1 727,741 - Pf1N1B4_630 0.46 -0.9 727,741 - Pf1N1B4_630 0.46 +1.8 727,744 - Pf1N1B4_630 0.47 -0.4 727,744 - Pf1N1B4_630 0.47 +0.2 727,744 - Pf1N1B4_630 0.47 +0.0 727,744 - Pf1N1B4_630 0.47 -0.5 727,867 + Pf1N1B4_630 0.63 +2.5 727,901 + Pf1N1B4_630 0.67 -1.1 727,902 - Pf1N1B4_630 0.67 +0.4 728,077 - +0.3 728,114 - -1.9 728,114 - -0.9 728,114 - -0.6 728,114 - -1.9 728,114 - -1.1 728,114 - -0.6 728,114 - +0.4 728,129 - -0.3 728,129 - -1.6 728,205 - +0.5 728,205 - -0.2 728,205 - -0.9 728,205 - -1.1 728,205 - +0.9 728,205 - -0.1
Or see this region's nucleotide sequence