Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6030 and Pf1N1B4_6031 overlap by 4 nucleotides Pf1N1B4_6031 and Pf1N1B4_6032 are separated by 56 nucleotides Pf1N1B4_6032 and Pf1N1B4_6033 are separated by 3 nucleotides Pf1N1B4_6033 and Pf1N1B4_6034 overlap by 4 nucleotides
Pf1N1B4_6030 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 494,561 to 494,965
_6030
Pf1N1B4_6031 - Ribokinase (EC 2.7.1.15) <small>(from data)</small>, at 494,962 to 495,879
_6031
Pf1N1B4_6032 - Ribose operon repressor, at 495,936 to 496,955
_6032
Pf1N1B4_6033 - D-ribose ABC transporter, permease component RbsC <small>(from data)</small>, at 496,959 to 497,936
_6033
Pf1N1B4_6034 - D-ribose ABC transporter, ATPase component RbsA <small>(from data)</small>, at 497,933 to 499,486
_6034
Position (kb) 495
496
497 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 494.974 kb on - strand at 494.983 kb on + strand at 494.983 kb on + strand at 494.983 kb on + strand at 494.984 kb on - strand at 494.984 kb on - strand at 494.984 kb on - strand at 495.493 kb on + strand, within Pf1N1B4_6031 at 495.494 kb on - strand, within Pf1N1B4_6031 at 495.681 kb on - strand, within Pf1N1B4_6031 at 495.857 kb on - strand at 495.857 kb on - strand at 495.857 kb on - strand at 495.860 kb on - strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.927 kb on - strand at 495.927 kb on - strand at 495.992 kb on + strand at 495.993 kb on - strand at 495.993 kb on - strand at 496.179 kb on - strand, within Pf1N1B4_6032 at 496.179 kb on - strand, within Pf1N1B4_6032 at 496.267 kb on - strand, within Pf1N1B4_6032 at 496.471 kb on + strand, within Pf1N1B4_6032 at 496.636 kb on + strand, within Pf1N1B4_6032 at 496.636 kb on + strand, within Pf1N1B4_6032 at 496.688 kb on - strand, within Pf1N1B4_6032 at 496.706 kb on - strand, within Pf1N1B4_6032 at 496.706 kb on - strand, within Pf1N1B4_6032 at 496.822 kb on + strand, within Pf1N1B4_6032 at 496.823 kb on - strand, within Pf1N1B4_6032 at 496.860 kb on + strand at 496.861 kb on - strand at 496.861 kb on - strand at 496.861 kb on - strand at 496.913 kb on - strand at 496.936 kb on - strand at 496.990 kb on + strand at 496.994 kb on + strand at 496.995 kb on - strand at 496.995 kb on - strand at 496.995 kb on - strand at 496.995 kb on - strand at 497.290 kb on + strand, within Pf1N1B4_6033 at 497.291 kb on - strand, within Pf1N1B4_6033 at 497.399 kb on + strand, within Pf1N1B4_6033 at 497.400 kb on - strand at 497.480 kb on - strand, within Pf1N1B4_6033 at 497.480 kb on - strand, within Pf1N1B4_6033 at 497.507 kb on - strand, within Pf1N1B4_6033 at 497.878 kb on + strand at 497.879 kb on - strand at 497.879 kb on - strand at 497.879 kb on - strand at 497.879 kb on - strand at 497.907 kb on - strand at 497.930 kb on + strand at 497.931 kb on - strand at 497.931 kb on - strand at 497.931 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 494,974 - -0.7 494,983 + -1.7 494,983 + -2.0 494,983 + +0.1 494,984 - +0.6 494,984 - -0.4 494,984 - +0.0 495,493 + Pf1N1B4_6031 0.58 +0.4 495,494 - Pf1N1B4_6031 0.58 +0.1 495,681 - Pf1N1B4_6031 0.78 -1.4 495,857 - -0.4 495,857 - -1.2 495,857 - +0.0 495,860 - -2.0 495,879 + -0.1 495,879 + +1.3 495,879 + +3.4 495,879 + +2.0 495,880 - +0.4 495,880 - +0.5 495,880 - +1.1 495,880 - -0.2 495,880 - -1.0 495,880 - -2.5 495,880 - -0.2 495,880 - -0.5 495,880 - -2.2 495,880 - -0.8 495,880 - +0.4 495,880 - -0.9 495,880 - +2.2 495,880 - -1.7 495,880 - +0.6 495,880 - -2.7 495,880 - -1.3 495,880 - -0.5 495,880 - -0.5 495,880 - +0.1 495,927 - -0.9 495,927 - -0.8 495,992 + -2.0 495,993 - +0.6 495,993 - -0.2 496,179 - Pf1N1B4_6032 0.24 -1.4 496,179 - Pf1N1B4_6032 0.24 -0.6 496,267 - Pf1N1B4_6032 0.32 +0.2 496,471 + Pf1N1B4_6032 0.52 +0.1 496,636 + Pf1N1B4_6032 0.69 -1.2 496,636 + Pf1N1B4_6032 0.69 -1.2 496,688 - Pf1N1B4_6032 0.74 -0.6 496,706 - Pf1N1B4_6032 0.75 +0.6 496,706 - Pf1N1B4_6032 0.75 -2.0 496,822 + Pf1N1B4_6032 0.87 +0.0 496,823 - Pf1N1B4_6032 0.87 -0.4 496,860 + -1.7 496,861 - +0.3 496,861 - -0.4 496,861 - +0.6 496,913 - -1.4 496,936 - +1.1 496,990 + +1.4 496,994 + -1.7 496,995 - -0.8 496,995 - -2.4 496,995 - -0.7 496,995 - -1.0 497,290 + Pf1N1B4_6033 0.34 +1.4 497,291 - Pf1N1B4_6033 0.34 +0.9 497,399 + Pf1N1B4_6033 0.45 -1.4 497,400 - +0.0 497,480 - Pf1N1B4_6033 0.53 +1.2 497,480 - Pf1N1B4_6033 0.53 -1.2 497,507 - Pf1N1B4_6033 0.56 +0.6 497,878 + -0.7 497,879 - +0.6 497,879 - +0.3 497,879 - +1.9 497,879 - +0.6 497,907 - -1.0 497,930 + -0.1 497,931 - -1.4 497,931 - -0.4 497,931 - +0.3
Or see this region's nucleotide sequence