Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6025 and Pf1N1B4_6026 are separated by 72 nucleotides Pf1N1B4_6026 and Pf1N1B4_6027 are separated by 165 nucleotides Pf1N1B4_6027 and Pf1N1B4_6028 are separated by 131 nucleotides Pf1N1B4_6028 and Pf1N1B4_6029 are separated by 38 nucleotides
Pf1N1B4_6025 - Cold shock protein CspG, at 492,049 to 492,261
_6025
Pf1N1B4_6026 - lipoprotein, putative, at 492,334 to 492,651
_6026
Pf1N1B4_6027 - FIG00955358: hypothetical protein, at 492,817 to 493,122
_6027
Pf1N1B4_6028 - FIG00954052: hypothetical protein, at 493,254 to 493,460
_6028
Pf1N1B4_6029 - uridine nucleosidase (EC 3.2.2.3) (from data) , at 493,499 to 494,527
_6029
Position (kb)
493
494 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 492.259 kb on + strand at 492.260 kb on - strand at 492.586 kb on - strand, within Pf1N1B4_6026 at 492.586 kb on - strand, within Pf1N1B4_6026 at 492.586 kb on - strand, within Pf1N1B4_6026 at 492.671 kb on - strand at 492.674 kb on + strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.675 kb on - strand at 492.806 kb on - strand at 492.806 kb on - strand at 492.806 kb on - strand at 492.806 kb on - strand at 492.806 kb on - strand at 492.821 kb on + strand at 492.822 kb on - strand at 492.822 kb on - strand at 492.822 kb on - strand at 492.834 kb on - strand at 492.834 kb on - strand at 492.924 kb on - strand, within Pf1N1B4_6027 at 492.924 kb on - strand, within Pf1N1B4_6027 at 492.990 kb on + strand, within Pf1N1B4_6027 at 492.991 kb on - strand, within Pf1N1B4_6027 at 493.005 kb on - strand, within Pf1N1B4_6027 at 493.005 kb on - strand, within Pf1N1B4_6027 at 493.164 kb on - strand at 493.182 kb on - strand at 493.182 kb on - strand at 493.182 kb on - strand at 493.182 kb on - strand at 493.264 kb on + strand at 493.314 kb on + strand, within Pf1N1B4_6028 at 493.314 kb on + strand, within Pf1N1B4_6028 at 493.353 kb on - strand at 493.422 kb on + strand, within Pf1N1B4_6028 at 493.423 kb on - strand at 493.423 kb on - strand, within Pf1N1B4_6028 at 493.434 kb on + strand, within Pf1N1B4_6028 at 493.434 kb on + strand, within Pf1N1B4_6028 at 493.434 kb on + strand, within Pf1N1B4_6028 at 493.434 kb on + strand, within Pf1N1B4_6028 at 493.435 kb on - strand, within Pf1N1B4_6028 at 493.435 kb on - strand at 493.435 kb on - strand, within Pf1N1B4_6028 at 493.435 kb on - strand, within Pf1N1B4_6028 at 493.435 kb on - strand, within Pf1N1B4_6028 at 493.436 kb on + strand, within Pf1N1B4_6028 at 493.437 kb on - strand, within Pf1N1B4_6028 at 493.500 kb on + strand at 493.501 kb on - strand at 493.501 kb on - strand at 493.501 kb on - strand at 493.501 kb on - strand at 493.600 kb on - strand at 493.600 kb on - strand at 493.652 kb on + strand, within Pf1N1B4_6029 at 493.652 kb on + strand, within Pf1N1B4_6029 at 493.653 kb on - strand at 493.696 kb on - strand, within Pf1N1B4_6029 at 493.709 kb on - strand, within Pf1N1B4_6029 at 493.764 kb on + strand, within Pf1N1B4_6029 at 493.765 kb on - strand, within Pf1N1B4_6029 at 493.765 kb on - strand, within Pf1N1B4_6029 at 493.765 kb on - strand, within Pf1N1B4_6029 at 493.875 kb on + strand, within Pf1N1B4_6029 at 493.876 kb on - strand, within Pf1N1B4_6029 at 494.118 kb on + strand, within Pf1N1B4_6029 at 494.119 kb on - strand, within Pf1N1B4_6029 at 494.119 kb on - strand, within Pf1N1B4_6029 at 494.209 kb on - strand, within Pf1N1B4_6029 at 494.209 kb on - strand at 494.209 kb on - strand, within Pf1N1B4_6029 at 494.351 kb on - strand, within Pf1N1B4_6029
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 492,259 + -2.3 492,260 - +0.0 492,586 - Pf1N1B4_6026 0.79 -0.4 492,586 - Pf1N1B4_6026 0.79 +1.6 492,586 - Pf1N1B4_6026 0.79 -1.4 492,671 - +1.4 492,674 + -2.0 492,675 - -1.4 492,675 - -1.1 492,675 - -0.5 492,675 - -0.1 492,675 - -1.4 492,675 - -1.8 492,675 - +0.2 492,675 - +0.0 492,675 - -0.2 492,675 - -0.4 492,675 - +0.3 492,806 - -0.1 492,806 - -0.4 492,806 - -2.4 492,806 - +0.0 492,806 - +0.0 492,821 + +1.4 492,822 - -1.2 492,822 - -1.0 492,822 - +0.2 492,834 - -1.2 492,834 - -0.4 492,924 - Pf1N1B4_6027 0.35 +0.4 492,924 - Pf1N1B4_6027 0.35 -2.2 492,990 + Pf1N1B4_6027 0.57 +0.4 492,991 - Pf1N1B4_6027 0.57 +0.0 493,005 - Pf1N1B4_6027 0.61 -0.3 493,005 - Pf1N1B4_6027 0.61 +1.5 493,164 - -3.0 493,182 - +0.2 493,182 - +2.2 493,182 - +0.5 493,182 - +1.0 493,264 + +0.0 493,314 + Pf1N1B4_6028 0.29 -2.0 493,314 + Pf1N1B4_6028 0.29 -0.2 493,353 - -1.0 493,422 + Pf1N1B4_6028 0.81 -1.0 493,423 - +0.6 493,423 - Pf1N1B4_6028 0.82 -1.4 493,434 + Pf1N1B4_6028 0.87 -0.8 493,434 + Pf1N1B4_6028 0.87 -1.4 493,434 + Pf1N1B4_6028 0.87 +0.3 493,434 + Pf1N1B4_6028 0.87 -1.7 493,435 - Pf1N1B4_6028 0.87 -1.7 493,435 - -1.0 493,435 - Pf1N1B4_6028 0.87 -2.0 493,435 - Pf1N1B4_6028 0.87 -2.4 493,435 - Pf1N1B4_6028 0.87 +0.4 493,436 + Pf1N1B4_6028 0.88 -0.0 493,437 - Pf1N1B4_6028 0.88 -0.8 493,500 + +0.0 493,501 - -0.4 493,501 - -0.7 493,501 - -0.4 493,501 - +0.9 493,600 - +0.6 493,600 - -1.2 493,652 + Pf1N1B4_6029 0.15 +1.6 493,652 + Pf1N1B4_6029 0.15 -2.0 493,653 - +0.6 493,696 - Pf1N1B4_6029 0.19 +1.4 493,709 - Pf1N1B4_6029 0.20 +0.4 493,764 + Pf1N1B4_6029 0.26 +0.6 493,765 - Pf1N1B4_6029 0.26 +0.6 493,765 - Pf1N1B4_6029 0.26 -1.5 493,765 - Pf1N1B4_6029 0.26 +0.0 493,875 + Pf1N1B4_6029 0.37 -0.1 493,876 - Pf1N1B4_6029 0.37 -1.0 494,118 + Pf1N1B4_6029 0.60 -0.6 494,119 - Pf1N1B4_6029 0.60 +0.2 494,119 - Pf1N1B4_6029 0.60 +1.6 494,209 - Pf1N1B4_6029 0.69 +0.1 494,209 - +1.2 494,209 - Pf1N1B4_6029 0.69 -0.3 494,351 - Pf1N1B4_6029 0.83 +0.6
Or see this region's nucleotide sequence