Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5857 and Pf1N1B4_5858 are separated by 340 nucleotides Pf1N1B4_5858 and Pf1N1B4_5859 are separated by 229 nucleotides
Pf1N1B4_5857 - Arginine N-succinyltransferase (EC 2.3.1.109), at 289,942 to 290,976
_5857
Pf1N1B4_5858 - carboxyphosphonoenolpyruvate phosphonomutase, putative, at 291,317 to 292,186
_5858
Pf1N1B4_5859 - Glutamate carboxypeptidase (EC 3.4.17.11), at 292,416 to 293,657
_5859
Position (kb) 291
292
293 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 290.340 kb on + strand, within Pf1N1B4_5857 at 290.681 kb on - strand, within Pf1N1B4_5857 at 291.153 kb on - strand at 291.153 kb on - strand at 291.247 kb on - strand at 291.302 kb on - strand at 291.317 kb on - strand at 291.350 kb on - strand at 291.383 kb on + strand at 291.931 kb on - strand, within Pf1N1B4_5858 at 291.950 kb on + strand, within Pf1N1B4_5858 at 291.951 kb on - strand, within Pf1N1B4_5858 at 292.028 kb on + strand, within Pf1N1B4_5858 at 292.029 kb on - strand, within Pf1N1B4_5858 at 292.053 kb on - strand, within Pf1N1B4_5858 at 292.053 kb on - strand, within Pf1N1B4_5858 at 292.123 kb on + strand at 292.123 kb on + strand at 292.123 kb on + strand at 292.123 kb on + strand at 292.123 kb on + strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.127 kb on + strand at 292.127 kb on + strand at 292.127 kb on + strand at 292.128 kb on - strand at 292.128 kb on - strand at 292.145 kb on + strand at 292.145 kb on + strand at 292.145 kb on + strand at 292.145 kb on + strand at 292.146 kb on - strand at 292.146 kb on - strand at 292.146 kb on - strand at 292.146 kb on - strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.167 kb on - strand at 292.168 kb on + strand at 292.168 kb on + strand at 292.168 kb on + strand at 292.168 kb on + strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.268 kb on + strand at 292.268 kb on + strand at 292.269 kb on - strand at 292.269 kb on - strand at 292.457 kb on - strand at 292.503 kb on - strand at 292.503 kb on - strand at 292.508 kb on + strand at 292.509 kb on - strand at 292.695 kb on - strand, within Pf1N1B4_5859 at 292.701 kb on - strand at 293.067 kb on + strand, within Pf1N1B4_5859 at 293.068 kb on - strand, within Pf1N1B4_5859
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 290,340 + Pf1N1B4_5857 0.38 -0.1 290,681 - Pf1N1B4_5857 0.71 -1.0 291,153 - +0.6 291,153 - -0.7 291,247 - -0.6 291,302 - -1.4 291,317 - -1.2 291,350 - -0.5 291,383 + +0.4 291,931 - Pf1N1B4_5858 0.71 +0.8 291,950 + Pf1N1B4_5858 0.73 +1.6 291,951 - Pf1N1B4_5858 0.73 +0.6 292,028 + Pf1N1B4_5858 0.82 +1.4 292,029 - Pf1N1B4_5858 0.82 -0.4 292,053 - Pf1N1B4_5858 0.85 +1.9 292,053 - Pf1N1B4_5858 0.85 +0.2 292,123 + -0.8 292,123 + +1.6 292,123 + -1.7 292,123 + +0.2 292,123 + +0.6 292,124 - +1.0 292,124 - -0.4 292,124 - -0.4 292,124 - +0.2 292,124 - +1.6 292,124 - +0.6 292,124 - -1.2 292,127 + +0.0 292,127 + +2.9 292,127 + +0.2 292,128 - +0.9 292,128 - +1.6 292,145 + -0.7 292,145 + +0.3 292,145 + -1.2 292,145 + +1.3 292,146 - +0.1 292,146 - +0.2 292,146 - +0.0 292,146 - -0.8 292,166 + -0.4 292,166 + -0.6 292,166 + -0.2 292,166 + +0.9 292,166 + -0.4 292,167 - +1.9 292,168 + +1.5 292,168 + +1.3 292,168 + +0.1 292,168 + +0.1 292,169 - +0.0 292,169 - +0.4 292,169 - +0.6 292,169 - +0.0 292,169 - +1.0 292,268 + -1.0 292,268 + +1.1 292,269 - -2.0 292,269 - -1.1 292,457 - -0.4 292,503 - +0.6 292,503 - +0.2 292,508 + +1.8 292,509 - -1.0 292,695 - Pf1N1B4_5859 0.22 -0.4 292,701 - +1.6 293,067 + Pf1N1B4_5859 0.52 +0.6 293,068 - Pf1N1B4_5859 0.52 +0.6
Or see this region's nucleotide sequence